Macaque stool associated virus 11
Average proteome isoelectric point is 7.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345MRS2|A0A345MRS2_9VIRU Rep protein OS=Macaque stool associated virus 11 OX=2499233 PE=4 SV=1
MM1 pKa = 7.17 VFVKK5 pKa = 10.66 VSEE8 pKa = 4.44 TYY10 pKa = 10.76 DD11 pKa = 3.42 LSTVPNKK18 pKa = 9.86 MGVIGVHH25 pKa = 5.69 TPDD28 pKa = 4.46 ASLISANWPGLLMNCKK44 pKa = 9.29 KK45 pKa = 10.21 FRR47 pKa = 11.84 YY48 pKa = 9.11 VEE50 pKa = 4.35 CNVTLACASMLPADD64 pKa = 4.41 PLQIGTEE71 pKa = 4.1 SGDD74 pKa = 3.41 IAPQDD79 pKa = 3.6 MFNPILYY86 pKa = 9.8 RR87 pKa = 11.84 AVSNQTMDD95 pKa = 3.89 SIEE98 pKa = 4.18 HH99 pKa = 6.69 RR100 pKa = 11.84 LADD103 pKa = 3.31 MMYY106 pKa = 10.28 AGEE109 pKa = 4.17 GSYY112 pKa = 10.74 NVNGPSVLEE121 pKa = 4.17 SQDD124 pKa = 3.7 GILPLTKK131 pKa = 10.4 NHH133 pKa = 6.13 WDD135 pKa = 3.24 VYY137 pKa = 11.03 YY138 pKa = 11.11 GLLSNRR144 pKa = 11.84 HH145 pKa = 4.76 GWRR148 pKa = 11.84 TAMPQQGLSMNGLAPLVHH166 pKa = 6.42 EE167 pKa = 4.54 RR168 pKa = 11.84 LYY170 pKa = 11.0 AYY172 pKa = 9.61 GVQNAIGTSNNGAVVDD188 pKa = 4.0 VLSNGTNDD196 pKa = 3.62 SGQTTIASFTNPPMVGRR213 pKa = 11.84 AHH215 pKa = 7.36 PMPSFPTVALAVRR228 pKa = 11.84 DD229 pKa = 3.89 NGVQQMNAWEE239 pKa = 4.27 NLPGDD244 pKa = 3.99 TAVSSTFSKK253 pKa = 10.44 IQIDD257 pKa = 4.13 QTSMPYY263 pKa = 9.66 IPPVFVACIIMPPAKK278 pKa = 10.26 LNRR281 pKa = 11.84 LYY283 pKa = 11.39 YY284 pKa = 10.36 RR285 pKa = 11.84 MNVEE289 pKa = 3.62 WTVEE293 pKa = 4.05 FTEE296 pKa = 4.16 IRR298 pKa = 11.84 PISEE302 pKa = 3.47 IMGFSALEE310 pKa = 3.78 EE311 pKa = 4.29 LGKK314 pKa = 8.99 FTYY317 pKa = 8.64 FTDD320 pKa = 3.81 YY321 pKa = 11.02 VQAKK325 pKa = 9.86 SLSTTMNTVDD335 pKa = 3.79 VASMDD340 pKa = 3.33 IEE342 pKa = 4.19 KK343 pKa = 10.85 VMM345 pKa = 4.58
Molecular weight: 37.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.752
IPC2_protein 4.902
IPC_protein 4.838
Toseland 4.736
ProMoST 4.965
Dawson 4.813
Bjellqvist 4.952
Wikipedia 4.698
Rodwell 4.711
Grimsley 4.647
Solomon 4.813
Lehninger 4.774
Nozaki 4.927
DTASelect 5.105
Thurlkill 4.736
EMBOSS 4.736
Sillero 4.991
Patrickios 3.338
IPC_peptide 4.825
IPC2_peptide 4.991
IPC2.peptide.svr19 4.922
Protein with the highest isoelectric point:
>tr|A0A345MRS1|A0A345MRS1_9VIRU Putative capsid protein OS=Macaque stool associated virus 11 OX=2499233 PE=4 SV=1
MM1 pKa = 7.73 SYY3 pKa = 10.78 NADD6 pKa = 3.23 VLKK9 pKa = 10.58 RR10 pKa = 11.84 YY11 pKa = 9.5 ISSRR15 pKa = 11.84 LGAYY19 pKa = 7.6 WDD21 pKa = 3.69 YY22 pKa = 8.57 WTEE25 pKa = 4.33 VPTTWGQFKK34 pKa = 10.31 AQSVLGGVFPVAQALYY50 pKa = 10.22 KK51 pKa = 10.76 VRR53 pKa = 11.84 DD54 pKa = 3.87 TEE56 pKa = 4.33 NKK58 pKa = 9.7 YY59 pKa = 10.89 NDD61 pKa = 3.6 YY62 pKa = 11.13 YY63 pKa = 11.46 KK64 pKa = 10.01 NQPYY68 pKa = 10.56 AGFPRR73 pKa = 11.84 YY74 pKa = 9.07 PSLTDD79 pKa = 3.23 YY80 pKa = 11.08 AQVYY84 pKa = 9.51 SYY86 pKa = 11.65 ASSSVMNFVSDD97 pKa = 3.94 NIGRR101 pKa = 11.84 LYY103 pKa = 10.47 RR104 pKa = 11.84 SKK106 pKa = 11.38
Molecular weight: 12.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.673
IPC2_protein 9.107
IPC_protein 9.121
Toseland 9.063
ProMoST 9.209
Dawson 9.443
Bjellqvist 9.253
Wikipedia 9.736
Rodwell 9.487
Grimsley 9.604
Solomon 9.531
Lehninger 9.458
Nozaki 9.004
DTASelect 9.253
Thurlkill 9.209
EMBOSS 9.502
Sillero 9.355
Patrickios 1.113
IPC_peptide 9.502
IPC2_peptide 8.024
IPC2.peptide.svr19 7.973
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
712
106
345
237.3
27.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.039 ± 1.052
1.264 ± 0.356
5.478 ± 0.362
4.916 ± 0.996
3.511 ± 0.203
6.461 ± 0.191
1.966 ± 0.58
6.32 ± 1.597
5.758 ± 1.981
6.601 ± 0.584
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
4.073 ± 1.075
5.337 ± 0.954
5.337 ± 0.861
3.792 ± 0.227
4.635 ± 0.917
6.882 ± 1.3
6.32 ± 0.452
7.303 ± 1.12
2.669 ± 0.823
5.337 ± 1.796
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here