Beluga whale coronavirus SW1
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B2BW42|B2BW42_9GAMC ORF 9 OS=Beluga whale coronavirus SW1 OX=694015 PE=4 SV=1
MM1 pKa = 6.99 YY2 pKa = 10.45 VVLLALFLYY11 pKa = 10.85 LLVNEE16 pKa = 4.45 EE17 pKa = 4.1 TPAIINGLSIIVFILLLEE35 pKa = 4.84 EE36 pKa = 4.44 CDD38 pKa = 3.83 KK39 pKa = 10.72 MHH41 pKa = 7.63 PLYY44 pKa = 10.31 CYY46 pKa = 10.36 CRR48 pKa = 11.84 MIEE51 pKa = 3.97 IPGDD55 pKa = 3.52 GFLNN59 pKa = 3.77
Molecular weight: 6.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.246
IPC2_protein 4.406
IPC_protein 4.139
Toseland 3.999
ProMoST 4.266
Dawson 4.088
Bjellqvist 4.24
Wikipedia 3.973
Rodwell 3.986
Grimsley 3.923
Solomon 4.062
Lehninger 4.012
Nozaki 4.228
DTASelect 4.291
Thurlkill 4.037
EMBOSS 3.986
Sillero 4.253
Patrickios 0.006
IPC_peptide 4.062
IPC2_peptide 4.24
IPC2.peptide.svr19 4.176
Protein with the highest isoelectric point:
>tr|B2BW38|B2BW38_9GAMC ORF 5c OS=Beluga whale coronavirus SW1 OX=694015 PE=4 SV=1
MM1 pKa = 7.67 GNFLSNIFTVQVSTNHH17 pKa = 5.89 NAHH20 pKa = 5.91 VVSAGHH26 pKa = 6.76 DD27 pKa = 3.29 ASLAASASNMQMHH40 pKa = 6.65 FSVTLVITSMLMFVIIIVMFLMLCSIRR67 pKa = 11.84 RR68 pKa = 11.84 HH69 pKa = 5.47 LMPNRR74 pKa = 11.84 GRR76 pKa = 11.84 SLIGRR81 pKa = 11.84 ATRR84 pKa = 11.84 AIRR87 pKa = 11.84 RR88 pKa = 11.84 TFKK91 pKa = 10.57 SRR93 pKa = 11.84 KK94 pKa = 9.5 GGGKK98 pKa = 10.0 DD99 pKa = 3.34 MEE101 pKa = 4.42 EE102 pKa = 3.89 LQPLNNQIKK111 pKa = 9.65 HH112 pKa = 5.79 SSVEE116 pKa = 4.1 FKK118 pKa = 11.13 KK119 pKa = 10.86 NLLTMINEE127 pKa = 3.76 VDD129 pKa = 3.62 KK130 pKa = 11.44 IEE132 pKa = 4.37 ASRR135 pKa = 11.84 KK136 pKa = 8.58 EE137 pKa = 3.84 EE138 pKa = 3.9 PFYY141 pKa = 11.39 EE142 pKa = 4.47 EE143 pKa = 4.71 IKK145 pKa = 10.69 LKK147 pKa = 10.64 PSTKK151 pKa = 9.74 KK152 pKa = 10.41 YY153 pKa = 10.01 RR154 pKa = 11.84 APQPPKK160 pKa = 10.03 ISSTPIYY167 pKa = 9.59 INHH170 pKa = 6.13 VGG172 pKa = 3.18
Molecular weight: 19.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.185
IPC2_protein 9.487
IPC_protein 9.589
Toseland 10.511
ProMoST 10.028
Dawson 10.613
Bjellqvist 10.204
Wikipedia 10.73
Rodwell 11.14
Grimsley 10.643
Solomon 10.643
Lehninger 10.628
Nozaki 10.482
DTASelect 10.204
Thurlkill 10.496
EMBOSS 10.891
Sillero 10.526
Patrickios 10.877
IPC_peptide 10.657
IPC2_peptide 8.726
IPC2.peptide.svr19 8.597
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14
0
14
14128
59
6663
1009.1
112.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.106 ± 0.333
2.803 ± 0.184
5.514 ± 0.211
5.627 ± 0.307
4.891 ± 0.327
6.689 ± 0.219
1.925 ± 0.211
5.21 ± 0.401
6.264 ± 0.366
8.175 ± 0.194
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.492 ± 0.117
5.153 ± 0.369
3.928 ± 0.201
3.157 ± 0.237
3.539 ± 0.348
7.106 ± 0.199
5.33 ± 0.24
9.11 ± 0.602
1.097 ± 0.103
4.884 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here