Beluga whale coronavirus SW1 
Average proteome isoelectric point is 6.49 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|B2BW42|B2BW42_9GAMC ORF 9 OS=Beluga whale coronavirus SW1 OX=694015 PE=4 SV=1 
MM1 pKa = 6.99  YY2 pKa = 10.45  VVLLALFLYY11 pKa = 10.85  LLVNEE16 pKa = 4.45  EE17 pKa = 4.1  TPAIINGLSIIVFILLLEE35 pKa = 4.84  EE36 pKa = 4.44  CDD38 pKa = 3.83  KK39 pKa = 10.72  MHH41 pKa = 7.63  PLYY44 pKa = 10.31  CYY46 pKa = 10.36  CRR48 pKa = 11.84  MIEE51 pKa = 3.97  IPGDD55 pKa = 3.52  GFLNN59 pKa = 3.77   
 Molecular weight: 6.79 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  4.246 
IPC2_protein 4.406 
IPC_protein 4.139 
Toseland    3.999 
ProMoST     4.266 
Dawson      4.088 
Bjellqvist  4.24 
Wikipedia   3.973 
Rodwell     3.986 
Grimsley    3.923 
Solomon     4.062 
Lehninger   4.012 
Nozaki      4.228 
DTASelect   4.291 
Thurlkill   4.037 
EMBOSS      3.986 
Sillero     4.253 
Patrickios  0.006 
IPC_peptide 4.062 
IPC2_peptide  4.24 
IPC2.peptide.svr19  4.176 
 Protein with the highest isoelectric point: 
>tr|B2BW38|B2BW38_9GAMC ORF 5c OS=Beluga whale coronavirus SW1 OX=694015 PE=4 SV=1 
MM1 pKa = 7.67  GNFLSNIFTVQVSTNHH17 pKa = 5.89  NAHH20 pKa = 5.91  VVSAGHH26 pKa = 6.76  DD27 pKa = 3.29  ASLAASASNMQMHH40 pKa = 6.65  FSVTLVITSMLMFVIIIVMFLMLCSIRR67 pKa = 11.84  RR68 pKa = 11.84  HH69 pKa = 5.47  LMPNRR74 pKa = 11.84  GRR76 pKa = 11.84  SLIGRR81 pKa = 11.84  ATRR84 pKa = 11.84  AIRR87 pKa = 11.84  RR88 pKa = 11.84  TFKK91 pKa = 10.57  SRR93 pKa = 11.84  KK94 pKa = 9.5  GGGKK98 pKa = 10.0  DD99 pKa = 3.34  MEE101 pKa = 4.42  EE102 pKa = 3.89  LQPLNNQIKK111 pKa = 9.65  HH112 pKa = 5.79  SSVEE116 pKa = 4.1  FKK118 pKa = 11.13  KK119 pKa = 10.86  NLLTMINEE127 pKa = 3.76  VDD129 pKa = 3.62  KK130 pKa = 11.44  IEE132 pKa = 4.37  ASRR135 pKa = 11.84  KK136 pKa = 8.58  EE137 pKa = 3.84  EE138 pKa = 3.9  PFYY141 pKa = 11.39  EE142 pKa = 4.47  EE143 pKa = 4.71  IKK145 pKa = 10.69  LKK147 pKa = 10.64  PSTKK151 pKa = 9.74  KK152 pKa = 10.41  YY153 pKa = 10.01  RR154 pKa = 11.84  APQPPKK160 pKa = 10.03  ISSTPIYY167 pKa = 9.59  INHH170 pKa = 6.13  VGG172 pKa = 3.18   
 Molecular weight: 19.44 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.185 
IPC2_protein 9.487 
IPC_protein 9.589 
Toseland    10.511 
ProMoST     10.028 
Dawson      10.613 
Bjellqvist  10.204 
Wikipedia   10.73 
Rodwell     11.14 
Grimsley    10.643 
Solomon     10.643 
Lehninger   10.628 
Nozaki      10.482 
DTASelect   10.204 
Thurlkill   10.496 
EMBOSS      10.891 
Sillero     10.526 
Patrickios  10.877 
IPC_peptide 10.657 
IPC2_peptide  8.726 
IPC2.peptide.svr19  8.597 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        14 
 
        
        0
 
        
        14 
         
        14128
 
        59
 
        6663
 
        1009.1
 
        112.58
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        7.106 ± 0.333
2.803 ± 0.184
 
        5.514 ± 0.211
5.627 ± 0.307
 
        4.891 ± 0.327
6.689 ± 0.219
 
        1.925 ± 0.211
5.21 ± 0.401
       
        6.264 ± 0.366
8.175 ± 0.194
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        2.492 ± 0.117
5.153 ± 0.369
 
        3.928 ± 0.201
3.157 ± 0.237
 
        3.539 ± 0.348
7.106 ± 0.199
 
        5.33 ± 0.24
9.11 ± 0.602
       
        1.097 ± 0.103
4.884 ± 0.177
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here