Landjia virus
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D3R1U5|A0A0D3R1U5_9RHAB Uncharacterized protein OS=Landjia virus OX=1272947 PE=4 SV=1
MM1 pKa = 7.16 EE2 pKa = 4.69 QEE4 pKa = 3.97 QQINCPYY11 pKa = 10.89 DD12 pKa = 3.28 WDD14 pKa = 4.09 AMSNSLKK21 pKa = 10.48 QADD24 pKa = 3.9 NEE26 pKa = 4.45 EE27 pKa = 4.04 EE28 pKa = 5.32 DD29 pKa = 4.5 EE30 pKa = 4.59 LCTAPSKK37 pKa = 10.76 PSILPTSDD45 pKa = 3.74 SDD47 pKa = 3.42 WAEE50 pKa = 3.96 SVMSPQEE57 pKa = 3.92 PCPEE61 pKa = 4.36 KK62 pKa = 10.83 EE63 pKa = 4.83 DD64 pKa = 4.07 DD65 pKa = 5.55 QSDD68 pKa = 3.65 DD69 pKa = 4.36 DD70 pKa = 4.48 YY71 pKa = 11.85 NEE73 pKa = 3.99 PVEE76 pKa = 4.48 DD77 pKa = 3.88 VRR79 pKa = 11.84 SSLDD83 pKa = 3.53 TLVHH87 pKa = 6.1 VPDD90 pKa = 4.1 HH91 pKa = 6.05 LSIRR95 pKa = 11.84 EE96 pKa = 3.93 EE97 pKa = 3.76 VRR99 pKa = 11.84 LIGDD103 pKa = 4.07 IQQLVYY109 pKa = 11.18 VLTNGQKK116 pKa = 10.83 KK117 pKa = 8.75 MDD119 pKa = 3.49 VMTKK123 pKa = 9.27 EE124 pKa = 4.09 GKK126 pKa = 10.21 KK127 pKa = 9.88 ILILNDD133 pKa = 2.81 INTPSTKK140 pKa = 10.37 SKK142 pKa = 10.51 VPIRR146 pKa = 11.84 KK147 pKa = 8.41 EE148 pKa = 3.58 RR149 pKa = 11.84 ALDD152 pKa = 3.74 DD153 pKa = 4.42 AGCIHH158 pKa = 7.78 LARR161 pKa = 11.84 VVQDD165 pKa = 3.5 LGKK168 pKa = 10.38 GIKK171 pKa = 9.38 VNKK174 pKa = 9.46 ILGGVSTLTWRR185 pKa = 11.84 SLLIDD190 pKa = 4.65 PDD192 pKa = 5.33 DD193 pKa = 4.01 IDD195 pKa = 3.64 RR196 pKa = 11.84 SKK198 pKa = 11.25 AYY200 pKa = 9.43 TSAASAYY207 pKa = 9.34 KK208 pKa = 10.3 AIIKK212 pKa = 9.42 LSPNYY217 pKa = 10.35 SIIKK221 pKa = 9.87 ARR223 pKa = 11.84 YY224 pKa = 6.86 KK225 pKa = 10.62 CPYY228 pKa = 9.19
Molecular weight: 25.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.723
IPC2_protein 4.711
IPC_protein 4.647
Toseland 4.482
ProMoST 4.774
Dawson 4.622
Bjellqvist 4.762
Wikipedia 4.507
Rodwell 4.495
Grimsley 4.393
Solomon 4.609
Lehninger 4.571
Nozaki 4.724
DTASelect 4.927
Thurlkill 4.495
EMBOSS 4.533
Sillero 4.774
Patrickios 4.012
IPC_peptide 4.622
IPC2_peptide 4.762
IPC2.peptide.svr19 4.743
Protein with the highest isoelectric point:
>tr|A0A0D3R1F1|A0A0D3R1F1_9RHAB Matrix OS=Landjia virus OX=1272947 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 10.54 KK3 pKa = 10.44 KK4 pKa = 9.62 MNSVQHH10 pKa = 5.85 HH11 pKa = 5.78 RR12 pKa = 11.84 NHH14 pKa = 6.55 RR15 pKa = 11.84 FFRR18 pKa = 11.84 HH19 pKa = 5.49 LTPIGQSLLCLHH31 pKa = 7.06 RR32 pKa = 11.84 NHH34 pKa = 6.46 AQRR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 MISQMMITMNPWRR52 pKa = 11.84 MLEE55 pKa = 3.99 ALLTLWFMCLIIYY68 pKa = 9.09 QLEE71 pKa = 4.31 RR72 pKa = 11.84 KK73 pKa = 9.65 SDD75 pKa = 3.23
Molecular weight: 9.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.184
IPC2_protein 10.028
IPC_protein 11.359
Toseland 11.535
ProMoST 11.959
Dawson 11.55
Bjellqvist 11.506
Wikipedia 11.989
Rodwell 11.433
Grimsley 11.594
Solomon 11.989
Lehninger 11.901
Nozaki 11.535
DTASelect 11.491
Thurlkill 11.535
EMBOSS 12.018
Sillero 11.535
Patrickios 11.199
IPC_peptide 12.003
IPC2_peptide 10.965
IPC2.peptide.svr19 9.351
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11
0
11
4482
75
2109
407.5
46.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.771 ± 0.356
1.606 ± 0.178
5.756 ± 0.416
5.466 ± 0.274
4.306 ± 0.456
5.221 ± 0.295
2.923 ± 0.348
8.501 ± 0.412
6.961 ± 0.493
9.705 ± 0.932
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.633 ± 0.461
6.158 ± 0.38
4.395 ± 0.398
3.525 ± 0.252
4.462 ± 0.35
8.099 ± 0.442
4.953 ± 0.379
5.109 ± 0.26
2.164 ± 0.198
4.284 ± 0.404
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here