Actinomadura madurae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Thermomonosporaceae; Actinomadura

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9393 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I5QSA7|A0A1I5QSA7_9ACTN Acetolactate synthase-1/2/3 large subunit OS=Actinomadura madurae OX=1993 GN=SAMN04489713_114236 PE=3 SV=1
MM1 pKa = 8.1RR2 pKa = 11.84KK3 pKa = 8.26LTRR6 pKa = 11.84NALAATAVMVTGLGLMSTPAYY27 pKa = 10.15AADD30 pKa = 3.91PWTVSPGGAISGTNLGPLTAIDD52 pKa = 4.16MTTGAAIVCDD62 pKa = 3.74SSTASGSAKK71 pKa = 10.3SGSNLPGAGIAQLDD85 pKa = 4.07SVSFSTPGNPNNWCTGPVGIVVQVTATGTPWQFNAQDD122 pKa = 3.59YY123 pKa = 11.05DD124 pKa = 3.95GAGVTTGTLTGVQASIHH141 pKa = 6.27GSDD144 pKa = 3.42EE145 pKa = 3.8CDD147 pKa = 3.01ATVAGPGSDD156 pKa = 2.88TGTIDD161 pKa = 3.33GTYY164 pKa = 10.56DD165 pKa = 3.37NSDD168 pKa = 2.89GTLRR172 pKa = 11.84VSGGNLEE179 pKa = 4.44VKK181 pKa = 9.53TVDD184 pKa = 3.65ADD186 pKa = 4.05CDD188 pKa = 3.64PSLINAGDD196 pKa = 3.69QVLLDD201 pKa = 3.45GAYY204 pKa = 9.5QVSPLQMITHH214 pKa = 7.4PP215 pKa = 4.17

Molecular weight: 21.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I5X8D2|A0A1I5X8D2_9ACTN NAD(P)-dependent dehydrogenase short-chain alcohol dehydrogenase family OS=Actinomadura madurae OX=1993 GN=SAMN04489713_12614 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 5.34KK15 pKa = 10.47KK16 pKa = 8.84HH17 pKa = 5.5GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSSRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.14GRR40 pKa = 11.84ARR42 pKa = 11.84IAVV45 pKa = 3.5

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9393

0

9393

3074860

29

7594

327.4

35.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.653 ± 0.041

0.779 ± 0.007

6.095 ± 0.02

5.69 ± 0.024

2.815 ± 0.014

9.728 ± 0.023

2.212 ± 0.011

3.398 ± 0.018

1.924 ± 0.019

10.326 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.883 ± 0.01

1.678 ± 0.012

6.274 ± 0.025

2.421 ± 0.014

8.714 ± 0.029

4.723 ± 0.017

5.633 ± 0.02

8.545 ± 0.021

1.502 ± 0.011

2.008 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski