Amino acid dipepetide frequency for Actinomadura madurae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.086AlaAla: 22.086 ± 0.138	1.098AlaCys: 1.098 ± 0.021	8.082AlaAsp: 8.082 ± 0.052	8.812AlaGlu: 8.812 ± 0.065	3.816AlaPhe: 3.816 ± 0.035	14.394AlaGly: 14.394 ± 0.086	2.664AlaHis: 2.664 ± 0.029	4.057AlaIle: 4.057 ± 0.037	2.569AlaLys: 2.569 ± 0.038	14.284AlaLeu: 14.284 ± 0.101	2.658AlaMet: 2.658 ± 0.032	1.827AlaAsn: 1.827 ± 0.025	7.007AlaPro: 7.007 ± 0.067	3.186AlaGln: 3.186 ± 0.036	11.257AlaArg: 11.257 ± 0.076	5.82AlaSer: 5.82 ± 0.046	6.342AlaThr: 6.342 ± 0.043	12.003AlaVal: 12.003 ± 0.075	1.946AlaTrp: 1.946 ± 0.026	2.619AlaTyr: 2.619 ± 0.029	0.0AlaXaa: 0.0 ± 0.0
Cys	1.029CysAla: 1.029 ± 0.022	0.109CysCys: 0.109 ± 0.006	0.51CysAsp: 0.51 ± 0.012	0.421CysGlu: 0.421 ± 0.012	0.224CysPhe: 0.224 ± 0.009	0.975CysGly: 0.975 ± 0.019	0.2CysHis: 0.2 ± 0.009	0.145CysIle: 0.145 ± 0.006	0.106CysLys: 0.106 ± 0.007	0.708CysLeu: 0.708 ± 0.015	0.129CysMet: 0.129 ± 0.007	0.131CysAsn: 0.131 ± 0.006	0.498CysPro: 0.498 ± 0.014	0.167CysGln: 0.167 ± 0.007	0.642CysArg: 0.642 ± 0.016	0.378CysSer: 0.378 ± 0.012	0.448CysThr: 0.448 ± 0.012	0.676CysVal: 0.676 ± 0.016	0.124CysTrp: 0.124 ± 0.006	0.167CysTyr: 0.167 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.832AspAla: 7.832 ± 0.055	0.38AspCys: 0.38 ± 0.011	3.933AspAsp: 3.933 ± 0.047	4.104AspGlu: 4.104 ± 0.036	1.535AspPhe: 1.535 ± 0.022	6.639AspGly: 6.639 ± 0.061	1.427AspHis: 1.427 ± 0.021	1.85AspIle: 1.85 ± 0.024	0.972AspLys: 0.972 ± 0.022	6.894AspLeu: 6.894 ± 0.051	0.919AspMet: 0.919 ± 0.019	0.791AspAsn: 0.791 ± 0.017	4.756AspPro: 4.756 ± 0.043	1.487AspGln: 1.487 ± 0.025	5.445AspArg: 5.445 ± 0.046	1.963AspSer: 1.963 ± 0.026	2.656AspThr: 2.656 ± 0.031	5.313AspVal: 5.313 ± 0.048	0.911AspTrp: 0.911 ± 0.019	1.146AspTyr: 1.146 ± 0.021	0.0AspXaa: 0.0 ± 0.0
Glu	7.246GluAla: 7.246 ± 0.062	0.366GluCys: 0.366 ± 0.011	2.932GluAsp: 2.932 ± 0.034	3.404GluGlu: 3.404 ± 0.042	1.511GluPhe: 1.511 ± 0.024	4.154GluGly: 4.154 ± 0.039	1.611GluHis: 1.611 ± 0.023	2.512GluIle: 2.512 ± 0.029	1.267GluLys: 1.267 ± 0.022	6.457GluLeu: 6.457 ± 0.056	1.041GluMet: 1.041 ± 0.017	1.04GluAsn: 1.04 ± 0.017	3.697GluPro: 3.697 ± 0.042	1.904GluGln: 1.904 ± 0.027	6.048GluArg: 6.048 ± 0.056	2.401GluSer: 2.401 ± 0.031	2.92GluThr: 2.92 ± 0.033	4.488GluVal: 4.488 ± 0.047	0.795GluTrp: 0.795 ± 0.017	1.103GluTyr: 1.103 ± 0.02	0.0GluXaa: 0.0 ± 0.0
Phe	3.907PheAla: 3.907 ± 0.041	0.285PheCys: 0.285 ± 0.01	2.024PheAsp: 2.024 ± 0.03	1.49PheGlu: 1.49 ± 0.025	0.878PhePhe: 0.878 ± 0.019	3.26PheGly: 3.26 ± 0.041	0.62PheHis: 0.62 ± 0.016	0.744PheIle: 0.744 ± 0.015	0.473PheLys: 0.473 ± 0.014	2.642PheLeu: 2.642 ± 0.028	0.446PheMet: 0.446 ± 0.012	0.53PheAsn: 0.53 ± 0.013	1.433PhePro: 1.433 ± 0.024	0.716PheGln: 0.716 ± 0.017	1.952PheArg: 1.952 ± 0.025	1.381PheSer: 1.381 ± 0.021	1.968PheThr: 1.968 ± 0.023	2.358PheVal: 2.358 ± 0.03	0.448PheTrp: 0.448 ± 0.014	0.591PheTyr: 0.591 ± 0.014	0.0PheXaa: 0.0 ± 0.0
Gly	11.334GlyAla: 11.334 ± 0.085	0.819GlyCys: 0.819 ± 0.018	5.818GlyAsp: 5.818 ± 0.045	5.378GlyGlu: 5.378 ± 0.05	2.967GlyPhe: 2.967 ± 0.036	9.565GlyGly: 9.565 ± 0.081	2.321GlyHis: 2.321 ± 0.031	3.426GlyIle: 3.426 ± 0.035	2.24GlyLys: 2.24 ± 0.032	9.822GlyLeu: 9.822 ± 0.062	2.146GlyMet: 2.146 ± 0.026	1.624GlyAsn: 1.624 ± 0.026	5.587GlyPro: 5.587 ± 0.049	2.46GlyGln: 2.46 ± 0.039	9.035GlyArg: 9.035 ± 0.068	4.811GlySer: 4.811 ± 0.045	6.107GlyThr: 6.107 ± 0.051	7.778GlyVal: 7.778 ± 0.064	1.759GlyTrp: 1.759 ± 0.026	2.281GlyTyr: 2.281 ± 0.03	0.0GlyXaa: 0.0 ± 0.0
His	2.701HisAla: 2.701 ± 0.031	0.203HisCys: 0.203 ± 0.008	1.4HisAsp: 1.4 ± 0.022	1.189HisGlu: 1.189 ± 0.022	0.598HisPhe: 0.598 ± 0.013	2.376HisGly: 2.376 ± 0.026	0.655HisHis: 0.655 ± 0.016	0.673HisIle: 0.673 ± 0.014	0.292HisLys: 0.292 ± 0.009	2.373HisLeu: 2.373 ± 0.032	0.381HisMet: 0.381 ± 0.013	0.341HisAsn: 0.341 ± 0.01	1.698HisPro: 1.698 ± 0.027	0.535HisGln: 0.535 ± 0.013	2.156HisArg: 2.156 ± 0.03	0.868HisSer: 0.868 ± 0.017	1.045HisThr: 1.045 ± 0.019	1.798HisVal: 1.798 ± 0.023	0.358HisTrp: 0.358 ± 0.012	0.483HisTyr: 0.483 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	5.217IleAla: 5.217 ± 0.044	0.297IleCys: 0.297 ± 0.01	2.402IleAsp: 2.402 ± 0.03	2.22IleGlu: 2.22 ± 0.032	0.808IlePhe: 0.808 ± 0.019	3.831IleGly: 3.831 ± 0.036	0.609IleHis: 0.609 ± 0.016	1.024IleIle: 1.024 ± 0.019	0.705IleLys: 0.705 ± 0.017	2.648IleLeu: 2.648 ± 0.031	0.546IleMet: 0.546 ± 0.016	0.676IleAsn: 0.676 ± 0.015	1.815IlePro: 1.815 ± 0.026	0.685IleGln: 0.685 ± 0.015	2.42IleArg: 2.42 ± 0.029	1.653IleSer: 1.653 ± 0.025	2.213IleThr: 2.213 ± 0.025	3.241IleVal: 3.241 ± 0.037	0.411IleTrp: 0.411 ± 0.012	0.559IleTyr: 0.559 ± 0.013	0.0IleXaa: 0.0 ± 0.0
Lys	2.686LysAla: 2.686 ± 0.033	0.107LysCys: 0.107 ± 0.006	1.149LysAsp: 1.149 ± 0.021	1.025LysGlu: 1.025 ± 0.017	0.405LysPhe: 0.405 ± 0.011	1.656LysGly: 1.656 ± 0.025	0.391LysHis: 0.391 ± 0.013	0.899LysIle: 0.899 ± 0.019	0.669LysLys: 0.669 ± 0.019	1.694LysLeu: 1.694 ± 0.027	0.351LysMet: 0.351 ± 0.012	0.448LysAsn: 0.448 ± 0.012	1.238LysPro: 1.238 ± 0.024	0.553LysGln: 0.553 ± 0.014	1.434LysArg: 1.434 ± 0.026	0.95LysSer: 0.95 ± 0.021	1.156LysThr: 1.156 ± 0.023	1.78LysVal: 1.78 ± 0.03	0.247LysTrp: 0.247 ± 0.009	0.402LysTyr: 0.402 ± 0.013	0.0LysXaa: 0.0 ± 0.0
Leu	15.601LeuAla: 15.601 ± 0.093	0.803LeuCys: 0.803 ± 0.018	7.08LeuAsp: 7.08 ± 0.053	4.856LeuGlu: 4.856 ± 0.048	2.683LeuPhe: 2.683 ± 0.035	9.58LeuGly: 9.58 ± 0.057	2.142LeuHis: 2.142 ± 0.029	3.455LeuIle: 3.455 ± 0.035	1.819LeuLys: 1.819 ± 0.03	10.889LeuLeu: 10.889 ± 0.081	1.692LeuMet: 1.692 ± 0.025	1.732LeuAsn: 1.732 ± 0.027	6.412LeuPro: 6.412 ± 0.049	2.025LeuGln: 2.025 ± 0.027	9.221LeuArg: 9.221 ± 0.066	5.107LeuSer: 5.107 ± 0.041	6.309LeuThr: 6.309 ± 0.048	8.789LeuVal: 8.789 ± 0.06	1.287LeuTrp: 1.287 ± 0.022	1.78LeuTyr: 1.78 ± 0.028	0.0LeuXaa: 0.0 ± 0.0
Met	2.459MetAla: 2.459 ± 0.034	0.14MetCys: 0.14 ± 0.007	0.967MetAsp: 0.967 ± 0.018	0.805MetGlu: 0.805 ± 0.015	0.527MetPhe: 0.527 ± 0.014	1.385MetGly: 1.385 ± 0.025	0.36MetHis: 0.36 ± 0.009	0.795MetIle: 0.795 ± 0.016	0.413MetLys: 0.413 ± 0.012	1.931MetLeu: 1.931 ± 0.026	0.338MetMet: 0.338 ± 0.012	0.446MetAsn: 0.446 ± 0.012	1.228MetPro: 1.228 ± 0.02	0.414MetGln: 0.414 ± 0.012	1.697MetArg: 1.697 ± 0.023	1.321MetSer: 1.321 ± 0.021	1.626MetThr: 1.626 ± 0.025	1.424MetVal: 1.424 ± 0.022	0.23MetTrp: 0.23 ± 0.008	0.322MetTyr: 0.322 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.149AsnAla: 2.149 ± 0.024	0.166AsnCys: 0.166 ± 0.007	0.927AsnAsp: 0.927 ± 0.02	0.823AsnGlu: 0.823 ± 0.017	0.456AsnPhe: 0.456 ± 0.013	1.822AsnGly: 1.822 ± 0.026	0.361AsnHis: 0.361 ± 0.011	0.621AsnIle: 0.621 ± 0.015	0.322AsnLys: 0.322 ± 0.011	1.676AsnLeu: 1.676 ± 0.023	0.3AsnMet: 0.3 ± 0.01	0.334AsnAsn: 0.334 ± 0.011	1.297AsnPro: 1.297 ± 0.023	0.434AsnGln: 0.434 ± 0.014	1.296AsnArg: 1.296 ± 0.02	0.709AsnSer: 0.709 ± 0.017	0.978AsnThr: 0.978 ± 0.021	1.459AsnVal: 1.459 ± 0.023	0.258AsnTrp: 0.258 ± 0.01	0.387AsnTyr: 0.387 ± 0.012	0.0AsnXaa: 0.0 ± 0.0
Pro	8.901ProAla: 8.901 ± 0.074	0.36ProCys: 0.36 ± 0.012	4.765ProAsp: 4.765 ± 0.044	4.375ProGlu: 4.375 ± 0.041	1.658ProPhe: 1.658 ± 0.027	7.268ProGly: 7.268 ± 0.059	1.287ProHis: 1.287 ± 0.02	1.595ProIle: 1.595 ± 0.023	1.152ProLys: 1.152 ± 0.022	5.285ProLeu: 5.285 ± 0.045	1.122ProMet: 1.122 ± 0.021	0.918ProAsn: 0.918 ± 0.02	4.436ProPro: 4.436 ± 0.065	1.443ProGln: 1.443 ± 0.028	4.4ProArg: 4.4 ± 0.042	3.402ProSer: 3.402 ± 0.04	2.703ProThr: 2.703 ± 0.031	5.439ProVal: 5.439 ± 0.046	0.92ProTrp: 0.92 ± 0.016	1.318ProTyr: 1.318 ± 0.024	0.0ProXaa: 0.0 ± 0.0
Gln	3.376GlnAla: 3.376 ± 0.034	0.161GlnCys: 0.161 ± 0.008	1.293GlnAsp: 1.293 ± 0.02	1.263GlnGlu: 1.263 ± 0.02	0.623GlnPhe: 0.623 ± 0.017	2.085GlnGly: 2.085 ± 0.03	0.507GlnHis: 0.507 ± 0.013	1.111GlnIle: 1.111 ± 0.02	0.536GlnLys: 0.536 ± 0.015	2.331GlnLeu: 2.331 ± 0.028	0.484GlnMet: 0.484 ± 0.012	0.491GlnAsn: 0.491 ± 0.013	1.387GlnPro: 1.387 ± 0.033	0.907GlnGln: 0.907 ± 0.028	2.197GlnArg: 2.197 ± 0.028	1.064GlnSer: 1.064 ± 0.019	1.181GlnThr: 1.181 ± 0.02	2.337GlnVal: 2.337 ± 0.03	0.37GlnTrp: 0.37 ± 0.01	0.506GlnTyr: 0.506 ± 0.014	0.0GlnXaa: 0.0 ± 0.0
Arg	10.66ArgAla: 10.66 ± 0.064	0.635ArgCys: 0.635 ± 0.016	5.137ArgAsp: 5.137 ± 0.045	5.014ArgGlu: 5.014 ± 0.042	2.659ArgPhe: 2.659 ± 0.031	6.33ArgGly: 6.33 ± 0.046	2.251ArgHis: 2.251 ± 0.029	3.387ArgIle: 3.387 ± 0.031	1.553ArgLys: 1.553 ± 0.025	9.974ArgLeu: 9.974 ± 0.07	1.982ArgMet: 1.982 ± 0.027	1.376ArgAsn: 1.376 ± 0.022	5.791ArgPro: 5.791 ± 0.054	2.149ArgGln: 2.149 ± 0.03	9.1ArgArg: 9.1 ± 0.079	4.061ArgSer: 4.061 ± 0.041	5.151ArgThr: 5.151 ± 0.038	6.552ArgVal: 6.552 ± 0.049	1.489ArgTrp: 1.489 ± 0.024	1.891ArgTyr: 1.891 ± 0.028	0.0ArgXaa: 0.0 ± 0.0
Ser	6.061SerAla: 6.061 ± 0.049	0.379SerCys: 0.379 ± 0.011	2.486SerAsp: 2.486 ± 0.028	2.268SerGlu: 2.268 ± 0.028	1.459SerPhe: 1.459 ± 0.021	5.876SerGly: 5.876 ± 0.048	0.92SerHis: 0.92 ± 0.017	1.518SerIle: 1.518 ± 0.025	0.882SerLys: 0.882 ± 0.021	4.57SerLeu: 4.57 ± 0.041	1.118SerMet: 1.118 ± 0.019	0.748SerAsn: 0.748 ± 0.016	3.174SerPro: 3.174 ± 0.037	1.051SerGln: 1.051 ± 0.019	3.796SerArg: 3.796 ± 0.033	2.507SerSer: 2.507 ± 0.04	2.612SerThr: 2.612 ± 0.029	3.925SerVal: 3.925 ± 0.041	0.814SerTrp: 0.814 ± 0.016	1.06SerTyr: 1.06 ± 0.017	0.0SerXaa: 0.0 ± 0.0
Thr	7.951ThrAla: 7.951 ± 0.059	0.449ThrCys: 0.449 ± 0.013	2.976ThrAsp: 2.976 ± 0.033	2.876ThrGlu: 2.876 ± 0.031	1.68ThrPhe: 1.68 ± 0.023	6.501ThrGly: 6.501 ± 0.052	1.036ThrHis: 1.036 ± 0.019	1.957ThrIle: 1.957 ± 0.027	0.959ThrLys: 0.959 ± 0.02	5.494ThrLeu: 5.494 ± 0.041	1.059ThrMet: 1.059 ± 0.021	0.864ThrAsn: 0.864 ± 0.018	3.735ThrPro: 3.735 ± 0.038	1.063ThrGln: 1.063 ± 0.019	4.126ThrArg: 4.126 ± 0.039	2.838ThrSer: 2.838 ± 0.028	3.221ThrThr: 3.221 ± 0.039	5.554ThrVal: 5.554 ± 0.048	0.853ThrTrp: 0.853 ± 0.015	1.139ThrTyr: 1.139 ± 0.022	0.0ThrXaa: 0.0 ± 0.0
Val	11.158ValAla: 11.158 ± 0.066	0.714ValCys: 0.714 ± 0.016	4.865ValAsp: 4.865 ± 0.046	4.663ValGlu: 4.663 ± 0.048	2.541ValPhe: 2.541 ± 0.029	6.389ValGly: 6.389 ± 0.058	1.989ValHis: 1.989 ± 0.025	3.103ValIle: 3.103 ± 0.037	1.607ValLys: 1.607 ± 0.028	9.716ValLeu: 9.716 ± 0.068	1.508ValMet: 1.508 ± 0.025	1.71ValAsn: 1.71 ± 0.026	5.625ValPro: 5.625 ± 0.05	2.035ValGln: 2.035 ± 0.026	7.532ValArg: 7.532 ± 0.054	4.218ValSer: 4.218 ± 0.039	5.523ValThr: 5.523 ± 0.044	7.906ValVal: 7.906 ± 0.067	1.109ValTrp: 1.109 ± 0.018	1.538ValTyr: 1.538 ± 0.024	0.0ValXaa: 0.0 ± 0.0
Trp	1.642TrpAla: 1.642 ± 0.025	0.136TrpCys: 0.136 ± 0.007	0.879TrpAsp: 0.879 ± 0.017	0.71TrpGlu: 0.71 ± 0.016	0.508TrpPhe: 0.508 ± 0.012	1.073TrpGly: 1.073 ± 0.017	0.378TrpHis: 0.378 ± 0.013	0.555TrpIle: 0.555 ± 0.013	0.327TrpLys: 0.327 ± 0.011	1.697TrpLeu: 1.697 ± 0.024	0.312TrpMet: 0.312 ± 0.009	0.378TrpAsn: 0.378 ± 0.011	0.863TrpPro: 0.863 ± 0.017	0.468TrpGln: 0.468 ± 0.013	1.516TrpArg: 1.516 ± 0.025	0.868TrpSer: 0.868 ± 0.018	1.08TrpThr: 1.08 ± 0.02	0.949TrpVal: 0.949 ± 0.017	0.344TrpTrp: 0.344 ± 0.011	0.337TrpTyr: 0.337 ± 0.011	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.529TyrAla: 2.529 ± 0.03	0.182TyrCys: 0.182 ± 0.008	1.327TyrAsp: 1.327 ± 0.025	1.201TyrGlu: 1.201 ± 0.021	0.608TyrPhe: 0.608 ± 0.014	2.119TyrGly: 2.119 ± 0.028	0.394TyrHis: 0.394 ± 0.011	0.554TyrIle: 0.554 ± 0.015	0.376TyrLys: 0.376 ± 0.012	2.177TyrLeu: 2.177 ± 0.027	0.296TyrMet: 0.296 ± 0.01	0.368TyrAsn: 0.368 ± 0.01	1.067TyrPro: 1.067 ± 0.022	0.529TyrGln: 0.529 ± 0.013	1.876TyrArg: 1.876 ± 0.029	0.906TyrSer: 0.906 ± 0.02	1.092TyrThr: 1.092 ± 0.019	1.678TyrVal: 1.678 ± 0.026	0.349TyrTrp: 0.349 ± 0.012	0.455TyrTyr: 0.455 ± 0.012	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 9393 proteins (3074860 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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