Micromonospora rhizosphaerae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Micromonospora

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6395 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C6RN58|A0A1C6RN58_9ACTN 50S ribosomal protein L25 OS=Micromonospora rhizosphaerae OX=568872 GN=rplY PE=3 SV=1
MM1 pKa = 6.87VTTTYY6 pKa = 10.36QVQGMTCGHH15 pKa = 6.54CVNSVSAEE23 pKa = 3.9VGALPGVSDD32 pKa = 3.9VQVDD36 pKa = 4.18LASGRR41 pKa = 11.84VTVTSEE47 pKa = 4.19SPLDD51 pKa = 3.66TDD53 pKa = 3.8TVRR56 pKa = 11.84AAVDD60 pKa = 3.1EE61 pKa = 4.62AGYY64 pKa = 11.0DD65 pKa = 3.52LVGAA69 pKa = 4.97

Molecular weight: 7.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C6S2E1|A0A1C6S2E1_9ACTN Uncharacterized protein OS=Micromonospora rhizosphaerae OX=568872 GN=GA0070624_2764 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.28VRR4 pKa = 11.84SSLRR8 pKa = 11.84GLKK11 pKa = 10.15RR12 pKa = 11.84KK13 pKa = 8.37MNSMVVRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84GKK24 pKa = 9.95VFVINKK30 pKa = 9.17ANPRR34 pKa = 11.84QKK36 pKa = 10.8ARR38 pKa = 11.84QGG40 pKa = 3.26

Molecular weight: 4.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6395

0

6395

2039900

39

3401

319.0

34.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.932 ± 0.053

0.745 ± 0.008

5.93 ± 0.028

5.231 ± 0.031

2.713 ± 0.02

9.274 ± 0.034

2.116 ± 0.014

3.374 ± 0.021

1.784 ± 0.022

10.571 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.674 ± 0.012

1.787 ± 0.017

6.333 ± 0.029

2.775 ± 0.016

8.531 ± 0.035

4.695 ± 0.021

5.887 ± 0.026

8.954 ± 0.027

1.609 ± 0.014

2.085 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski