Ochrobactrum phage POA1180

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A219VHA5|A0A219VHA5_9CAUD Uncharacterized protein OS=Ochrobactrum phage POA1180 OX=1897640 GN=POA1180_20 PE=4 SV=1
MM1 pKa = 7.4ALSVFVAGSRR11 pKa = 11.84VAFARR16 pKa = 11.84YY17 pKa = 9.51LMQAPLFVAVGSGDD31 pKa = 3.95AAWDD35 pKa = 3.78SLPDD39 pKa = 3.61PTPEE43 pKa = 5.04EE44 pKa = 4.1EE45 pKa = 4.42TQQLAALSQEE55 pKa = 4.36SALTNCIGVTRR66 pKa = 11.84VRR68 pKa = 11.84ARR70 pKa = 11.84SYY72 pKa = 10.63MKK74 pKa = 10.48ADD76 pKa = 3.71PNGDD80 pKa = 3.82IILSDD85 pKa = 3.72GSAWSVSSTEE95 pKa = 4.02TNTIRR100 pKa = 11.84LEE102 pKa = 4.19AKK104 pKa = 10.35LDD106 pKa = 3.49IGDD109 pKa = 3.72ATGDD113 pKa = 3.79SVRR116 pKa = 11.84EE117 pKa = 3.81TGVYY121 pKa = 10.29LDD123 pKa = 3.9TKK125 pKa = 10.33ISEE128 pKa = 4.48SVPAGQMFIPLADD141 pKa = 3.67VTSLGTLLQLHH152 pKa = 6.86RR153 pKa = 11.84FPPIVRR159 pKa = 11.84DD160 pKa = 3.81GTISQAINPYY170 pKa = 10.66LEE172 pKa = 4.09LL173 pKa = 5.78

Molecular weight: 18.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A219VHA3|A0A219VHA3_9CAUD Baseplate assembly protein OS=Ochrobactrum phage POA1180 OX=1897640 GN=POA1180_12 PE=4 SV=1
MM1 pKa = 7.54SEE3 pKa = 4.06SLDD6 pKa = 3.43RR7 pKa = 11.84RR8 pKa = 11.84PEE10 pKa = 3.75LRR12 pKa = 11.84PAIEE16 pKa = 4.25ALHH19 pKa = 6.25AFKK22 pKa = 10.7FNPPDD27 pKa = 3.24QIVPYY32 pKa = 10.29LVAEE36 pKa = 4.19YY37 pKa = 10.45GLNEE41 pKa = 4.03IADD44 pKa = 4.2YY45 pKa = 11.18LPDD48 pKa = 3.34LRR50 pKa = 11.84AVLRR54 pKa = 11.84EE55 pKa = 3.97GVSWQRR61 pKa = 11.84LIGTPAAVHH70 pKa = 6.49KK71 pKa = 10.09ALRR74 pKa = 11.84WINHH78 pKa = 6.74DD79 pKa = 3.23GDD81 pKa = 3.95IEE83 pKa = 4.12EE84 pKa = 4.85FPAKK88 pKa = 9.22KK89 pKa = 10.19RR90 pKa = 11.84KK91 pKa = 7.6WWWFQVHH98 pKa = 6.43LPFEE102 pKa = 4.35VRR104 pKa = 11.84NTDD107 pKa = 3.61FVRR110 pKa = 11.84PMTHH114 pKa = 6.44LVKK117 pKa = 10.59ASKK120 pKa = 10.02PLRR123 pKa = 11.84SEE125 pKa = 4.49FARR128 pKa = 11.84VTAGWDD134 pKa = 3.2VRR136 pKa = 11.84AFQLNKK142 pKa = 9.89HH143 pKa = 6.02RR144 pKa = 11.84LNGDD148 pKa = 3.18AFLNNWSGVRR158 pKa = 11.84RR159 pKa = 11.84SPGEE163 pKa = 3.61PVLSLRR169 pKa = 11.84VNQNVRR175 pKa = 11.84TKK177 pKa = 9.72FWPAPFVRR185 pKa = 11.84VRR187 pKa = 11.84THH189 pKa = 5.01TVITSFVRR197 pKa = 11.84ATYY200 pKa = 9.8QLPLRR205 pKa = 11.84QRR207 pKa = 11.84IAIFVASAGVLARR220 pKa = 11.84YY221 pKa = 9.35SDD223 pKa = 3.98PAIQTFQNAPFVHH236 pKa = 6.94DD237 pKa = 4.42RR238 pKa = 11.84FGKK241 pKa = 8.0PASRR245 pKa = 11.84AHH247 pKa = 6.46IGFSS251 pKa = 3.25

Molecular weight: 28.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

12853

50

1104

221.6

24.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.445 ± 0.606

0.646 ± 0.092

5.991 ± 0.252

6.691 ± 0.268

3.875 ± 0.224

7.734 ± 0.349

1.657 ± 0.129

6.263 ± 0.22

4.964 ± 0.321

8.356 ± 0.304

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.108 ± 0.167

3.4 ± 0.211

4.8 ± 0.261

3.563 ± 0.186

7.072 ± 0.327

5.648 ± 0.254

5.376 ± 0.307

6.73 ± 0.335

1.486 ± 0.129

2.194 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski