Sphingomonas oligophenolica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2977 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A502C3S4|A0A502C3S4_9SPHN Uncharacterized protein OS=Sphingomonas oligophenolica OX=301154 GN=EAH84_14385 PE=4 SV=1
MM1 pKa = 6.26QTYY4 pKa = 10.07LVRR7 pKa = 11.84LNEE10 pKa = 4.37AQHH13 pKa = 6.16HH14 pKa = 5.48PRR16 pKa = 11.84EE17 pKa = 4.17IVGIFAASSDD27 pKa = 3.48EE28 pKa = 4.3HH29 pKa = 7.47LADD32 pKa = 5.82MIDD35 pKa = 3.44EE36 pKa = 4.72CCDD39 pKa = 3.09VDD41 pKa = 5.09SIEE44 pKa = 4.82IADD47 pKa = 4.24LGAGGLYY54 pKa = 9.53WSGPVEE60 pKa = 4.09VTLPLTYY67 pKa = 10.5DD68 pKa = 3.5SEE70 pKa = 4.72TGDD73 pKa = 4.19GLRR76 pKa = 11.84LPPDD80 pKa = 3.7PSISEE85 pKa = 4.05DD86 pKa = 2.68WHH88 pKa = 7.32QIFYY92 pKa = 10.96DD93 pKa = 4.26ADD95 pKa = 4.02TFWRR99 pKa = 11.84ALEE102 pKa = 4.09PVV104 pKa = 3.29

Molecular weight: 11.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A502CIY0|A0A502CIY0_9SPHN Thymidylate synthase OS=Sphingomonas oligophenolica OX=301154 GN=thyA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.58GFRR19 pKa = 11.84SRR21 pKa = 11.84MATVGGRR28 pKa = 11.84SVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.0KK41 pKa = 10.58LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2977

0

2977

903567

29

1795

303.5

32.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.13 ± 0.069

0.729 ± 0.014

6.401 ± 0.043

4.727 ± 0.043

3.384 ± 0.029

8.866 ± 0.043

2.004 ± 0.022

5.219 ± 0.029

2.846 ± 0.038

9.579 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.404 ± 0.022

2.362 ± 0.03

5.333 ± 0.035

2.886 ± 0.025

7.407 ± 0.049

4.881 ± 0.034

5.818 ± 0.032

7.492 ± 0.034

1.365 ± 0.019

2.166 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski