Corynebacterium phage P1201
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A7IYG4|A7IYG4_9CAUD Gp97 OS=Corynebacterium phage P1201 OX=384848 PE=4 SV=1
MM1 pKa = 7.51 SIFHH5 pKa = 6.25 TVHH8 pKa = 7.08 DD9 pKa = 4.56 SSLTTIEE16 pKa = 4.41 CTTGEE21 pKa = 4.25 IVRR24 pKa = 11.84 VGYY27 pKa = 10.69 DD28 pKa = 3.64 EE29 pKa = 5.23 MPEE32 pKa = 4.56 CPLDD36 pKa = 3.31 WDD38 pKa = 3.94 VFGASACRR46 pKa = 11.84 RR47 pKa = 11.84 VDD49 pKa = 3.17 RR50 pKa = 11.84 HH51 pKa = 6.45 HH52 pKa = 7.63 DD53 pKa = 3.56 SDD55 pKa = 4.88 FLGDD59 pKa = 4.4 DD60 pKa = 4.08 DD61 pKa = 4.91 TVTAYY66 pKa = 10.29 RR67 pKa = 11.84 DD68 pKa = 3.43 HH69 pKa = 7.07 LADD72 pKa = 3.41 IDD74 pKa = 3.59 EE75 pKa = 4.62 WEE77 pKa = 4.41 NEE79 pKa = 3.94 VSEE82 pKa = 4.48 LANEE86 pKa = 4.16 KK87 pKa = 10.75 ADD89 pKa = 4.08 DD90 pKa = 4.0 EE91 pKa = 4.66 GVDD94 pKa = 4.08 VFDD97 pKa = 5.68 SNWNHH102 pKa = 5.47 TPEE105 pKa = 5.53 YY106 pKa = 9.34 MDD108 pKa = 2.99 IWQDD112 pKa = 3.31 VVLHH116 pKa = 5.32 YY117 pKa = 10.73 RR118 pKa = 11.84 PMPEE122 pKa = 3.87 CPVIVRR128 pKa = 11.84 RR129 pKa = 11.84 SGEE132 pKa = 3.89 CEE134 pKa = 3.82 AIVNKK139 pKa = 9.1 VQPDD143 pKa = 3.62 FMRR146 pKa = 11.84 GVVLADD152 pKa = 3.87 LAHH155 pKa = 6.97 DD156 pKa = 3.61 VLEE159 pKa = 4.79 TFEE162 pKa = 3.88 QWADD166 pKa = 3.39 GEE168 pKa = 4.75 VYY170 pKa = 9.81 MIEE173 pKa = 4.13 VEE175 pKa = 4.42 HH176 pKa = 6.94 PNGDD180 pKa = 3.27 TDD182 pKa = 4.38 VIGGIYY188 pKa = 10.39 GDD190 pKa = 4.21 MPTTEE195 pKa = 4.79 QDD197 pKa = 3.54 VQDD200 pKa = 4.05 YY201 pKa = 10.78 LL202 pKa = 3.85
Molecular weight: 23.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.761
IPC2_protein 3.923
IPC_protein 3.935
Toseland 3.719
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.834
Rodwell 3.757
Grimsley 3.63
Solomon 3.91
Lehninger 3.872
Nozaki 4.024
DTASelect 4.253
Thurlkill 3.77
EMBOSS 3.846
Sillero 4.05
Patrickios 1.914
IPC_peptide 3.91
IPC2_peptide 4.037
IPC2.peptide.svr19 3.934
Protein with the highest isoelectric point:
>tr|A7IY83|A7IY83_9CAUD Gp12 OS=Corynebacterium phage P1201 OX=384848 PE=4 SV=1
MM1 pKa = 7.59 CLAGVLSYY9 pKa = 10.37 PEE11 pKa = 4.2 SMRR14 pKa = 11.84 GSASSTLRR22 pKa = 11.84 IFKK25 pKa = 10.43 RR26 pKa = 11.84 SVWPSKK32 pKa = 10.4 RR33 pKa = 11.84 SRR35 pKa = 11.84 CPFDD39 pKa = 2.93 ISRR42 pKa = 11.84 PKK44 pKa = 10.39 RR45 pKa = 11.84 RR46 pKa = 11.84 FRR48 pKa = 11.84 PSTGILEE55 pKa = 4.44 GDD57 pKa = 4.03 LGHH60 pKa = 7.51 LSLDD64 pKa = 3.79 RR65 pKa = 11.84 QPSFAEE71 pKa = 3.82 VGFYY75 pKa = 10.48 SSKK78 pKa = 10.71 RR79 pKa = 11.84 PADD82 pKa = 3.27
Molecular weight: 9.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.619
IPC_protein 10.526
Toseland 10.716
ProMoST 10.467
Dawson 10.804
Bjellqvist 10.555
Wikipedia 11.038
Rodwell 10.935
Grimsley 10.847
Solomon 10.965
Lehninger 10.921
Nozaki 10.716
DTASelect 10.54
Thurlkill 10.716
EMBOSS 11.125
Sillero 10.745
Patrickios 10.716
IPC_peptide 10.965
IPC2_peptide 9.75
IPC2.peptide.svr19 8.577
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
95
0
95
21088
39
2127
222.0
24.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.502 ± 0.338
0.901 ± 0.142
6.217 ± 0.207
7.905 ± 0.3
3.874 ± 0.231
7.431 ± 0.412
1.683 ± 0.14
5.695 ± 0.16
5.828 ± 0.242
7.952 ± 0.21
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.442 ± 0.176
3.865 ± 0.163
4.472 ± 0.242
3.286 ± 0.167
5.856 ± 0.238
7.341 ± 0.249
5.539 ± 0.237
6.497 ± 0.199
1.674 ± 0.099
3.04 ± 0.245
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here