Mycobacterium virus Littlee

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Omegavirus

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 229 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G1D424|G1D424_9CAUD Uncharacterized protein OS=Mycobacterium virus Littlee OX=1034111 GN=139 PE=4 SV=1
MM1 pKa = 7.85SYY3 pKa = 10.0PHH5 pKa = 6.73HH6 pKa = 7.23AEE8 pKa = 4.18DD9 pKa = 5.04GEE11 pKa = 4.3LGAIYY16 pKa = 9.08VTLTPNPVVKK26 pKa = 9.5TVSLDD31 pKa = 3.5DD32 pKa = 3.69GAINVDD38 pKa = 3.4YY39 pKa = 10.97DD40 pKa = 3.86ADD42 pKa = 3.95GNAVGVEE49 pKa = 3.85ILLL52 pKa = 4.13

Molecular weight: 5.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G1D4B2|G1D4B2_9CAUD Uncharacterized protein OS=Mycobacterium virus Littlee OX=1034111 GN=228 PE=4 SV=1
MM1 pKa = 7.46SNATITDD8 pKa = 3.56VSADD12 pKa = 3.43LHH14 pKa = 5.94DD15 pKa = 5.58AGWQTVATRR24 pKa = 11.84TRR26 pKa = 11.84GTQAVVEE33 pKa = 4.1YY34 pKa = 9.54AQRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 7.97VWALVDD46 pKa = 3.38TRR48 pKa = 11.84TNDD51 pKa = 3.52VIGGTAVRR59 pKa = 11.84EE60 pKa = 3.92VRR62 pKa = 11.84AAEE65 pKa = 3.88MVRR68 pKa = 11.84EE69 pKa = 3.91SEE71 pKa = 3.86YY72 pKa = 10.44RR73 pKa = 11.84KK74 pKa = 9.59RR75 pKa = 11.84VRR77 pKa = 11.84AYY79 pKa = 8.08RR80 pKa = 11.84TLNTDD85 pKa = 2.74ARR87 pKa = 11.84KK88 pKa = 8.58GTDD91 pKa = 2.73WEE93 pKa = 4.2RR94 pKa = 11.84RR95 pKa = 11.84RR96 pKa = 3.85

Molecular weight: 10.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

229

0

229

34227

22

1610

149.5

16.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.96 ± 0.295

1.405 ± 0.134

6.495 ± 0.14

7.003 ± 0.286

3.132 ± 0.111

8.683 ± 0.404

2.22 ± 0.12

4.905 ± 0.133

3.997 ± 0.158

8.111 ± 0.189

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.472 ± 0.095

3.258 ± 0.107

5.504 ± 0.158

2.957 ± 0.127

6.889 ± 0.252

5.446 ± 0.165

5.426 ± 0.183

6.825 ± 0.219

2.308 ± 0.114

3.003 ± 0.123
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski