Tatlockia micdadei (Legionella micdadei)
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2989 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A098GF02|A0A098GF02_TATMI Integrating conjugative element protein OS=Tatlockia micdadei OX=451 GN=B6N58_11655 PE=4 SV=1
MM1 pKa = 7.26 SAINASPSSSSIFFSVSAADD21 pKa = 4.1 KK22 pKa = 9.87 VASLIAEE29 pKa = 4.14 EE30 pKa = 4.59 EE31 pKa = 4.21 NPNLNLRR38 pKa = 11.84 VFVTGGGCSGFQYY51 pKa = 11.02 GFTFDD56 pKa = 4.63 EE57 pKa = 5.59 KK58 pKa = 10.56 IQEE61 pKa = 4.56 DD62 pKa = 3.95 DD63 pKa = 3.73 TVIEE67 pKa = 4.27 QACSDD72 pKa = 3.62 GKK74 pKa = 11.25 SSVKK78 pKa = 10.59 LLVDD82 pKa = 3.58 SMSYY86 pKa = 10.49 QYY88 pKa = 11.17 LSEE91 pKa = 5.05 AEE93 pKa = 3.52 IDD95 pKa = 3.87 YY96 pKa = 11.35 VEE98 pKa = 4.39 NLQGAQFVIRR108 pKa = 11.84 NPNAKK113 pKa = 6.55 TTCGCGSSFSMGDD126 pKa = 3.23 DD127 pKa = 3.91 DD128 pKa = 5.54 EE129 pKa = 6.74 FIDD132 pKa = 5.29 GEE134 pKa = 4.35 EE135 pKa = 3.86 AA136 pKa = 3.63
Molecular weight: 14.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.755
IPC2_protein 3.821
IPC_protein 3.77
Toseland 3.579
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.668
Rodwell 3.605
Grimsley 3.49
Solomon 3.732
Lehninger 3.694
Nozaki 3.872
DTASelect 4.05
Thurlkill 3.63
EMBOSS 3.681
Sillero 3.884
Patrickios 1.863
IPC_peptide 3.732
IPC2_peptide 3.872
IPC2.peptide.svr19 3.804
Protein with the highest isoelectric point:
>tr|A0A098GK94|A0A098GK94_TATMI ATP-dependent DNA helicase RecG OS=Tatlockia micdadei OX=451 GN=recG PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLKK11 pKa = 10.18 RR12 pKa = 11.84 KK13 pKa = 8.83 RR14 pKa = 11.84 DD15 pKa = 3.29 HH16 pKa = 7.17 GFRR19 pKa = 11.84 QRR21 pKa = 11.84 MATRR25 pKa = 11.84 GGRR28 pKa = 11.84 LVLKK32 pKa = 10.32 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.84 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.442
IPC2_protein 10.95
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.093
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2989
0
2989
985229
37
4370
329.6
37.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.989 ± 0.047
1.189 ± 0.018
4.69 ± 0.027
6.122 ± 0.064
4.572 ± 0.038
6.088 ± 0.042
2.471 ± 0.024
7.304 ± 0.042
6.025 ± 0.045
11.18 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.282 ± 0.02
4.689 ± 0.033
4.238 ± 0.03
4.599 ± 0.036
4.539 ± 0.03
6.397 ± 0.033
5.186 ± 0.033
5.916 ± 0.038
1.137 ± 0.016
3.389 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here