Nocardioides sp. Root79

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; unclassified Nocardioides

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4673 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q8VQS4|A0A0Q8VQS4_9ACTN Uncharacterized protein OS=Nocardioides sp. Root79 OX=1736600 GN=ASE19_01695 PE=4 SV=1
MM1 pKa = 6.6VTTPAAQAAGQSISTTTNVGAPTGNGHH28 pKa = 6.65CLNPNTTSDD37 pKa = 3.65PTNCNAYY44 pKa = 10.1ASKK47 pKa = 11.25ADD49 pKa = 3.34VWLSNLPQGLPEE61 pKa = 4.52GDD63 pKa = 3.71YY64 pKa = 11.03FFAVSDD70 pKa = 4.62PGSQSDD76 pKa = 4.42PNDD79 pKa = 3.63GSDD82 pKa = 3.55GLLSAGTHH90 pKa = 5.21TDD92 pKa = 2.64RR93 pKa = 11.84SFRR96 pKa = 11.84IEE98 pKa = 4.13NGHH101 pKa = 4.31VTILGDD107 pKa = 3.57HH108 pKa = 6.88LKK110 pKa = 10.52FADD113 pKa = 3.8RR114 pKa = 11.84VQVFPFADD122 pKa = 3.62TTNGGGVYY130 pKa = 10.21NLSVCSLAGGYY141 pKa = 9.31PVVSSDD147 pKa = 3.54CTHH150 pKa = 7.58DD151 pKa = 3.64SFKK154 pKa = 11.29VGAAPVEE161 pKa = 4.31AKK163 pKa = 10.33PLTIVKK169 pKa = 9.78DD170 pKa = 3.41AAGTYY175 pKa = 10.34DD176 pKa = 4.25RR177 pKa = 11.84TYY179 pKa = 10.29TWDD182 pKa = 2.98IDD184 pKa = 3.82KK185 pKa = 11.3AVDD188 pKa = 3.79DD189 pKa = 4.29DD190 pKa = 4.12TLEE193 pKa = 4.23GRR195 pKa = 11.84EE196 pKa = 4.47GTSVTANYY204 pKa = 7.94TVTVEE209 pKa = 4.0HH210 pKa = 7.54DD211 pKa = 3.2GGEE214 pKa = 3.98VDD216 pKa = 4.51NIGVTGDD223 pKa = 2.94ITVFNPNGAAVTADD237 pKa = 3.48VTDD240 pKa = 3.69VLSDD244 pKa = 3.63GTVCTVDD251 pKa = 3.06GGTDD255 pKa = 3.3AVIPAMGDD263 pKa = 3.23TVLGYY268 pKa = 8.62TCDD271 pKa = 3.78LTSLPQGQLDD281 pKa = 3.69NTATVTWEE289 pKa = 3.85EE290 pKa = 3.98QTLGNTDD297 pKa = 3.19HH298 pKa = 7.21LAAGDD303 pKa = 3.72EE304 pKa = 4.45SFTYY308 pKa = 10.96DD309 pKa = 4.23DD310 pKa = 3.74VTFTGNDD317 pKa = 2.76IDD319 pKa = 3.89EE320 pKa = 5.74CIDD323 pKa = 3.33VDD325 pKa = 4.0DD326 pKa = 4.47TVEE329 pKa = 4.2GTLGTVCVGDD339 pKa = 4.43DD340 pKa = 3.62NPTEE344 pKa = 3.93LSYY347 pKa = 11.7DD348 pKa = 3.68RR349 pKa = 11.84TWNLDD354 pKa = 3.36TPGCQDD360 pKa = 3.36YY361 pKa = 11.5DD362 pKa = 3.54NTASFEE368 pKa = 4.45TNDD371 pKa = 3.65TGSTDD376 pKa = 3.64DD377 pKa = 4.03ASQSVEE383 pKa = 3.72VCTIDD388 pKa = 4.14VLDD391 pKa = 4.55ALTVSKK397 pKa = 10.26EE398 pKa = 3.94AEE400 pKa = 3.83GDD402 pKa = 3.6YY403 pKa = 8.0TTTYY407 pKa = 9.12KK408 pKa = 10.37WSVDD412 pKa = 3.21KK413 pKa = 11.47SVDD416 pKa = 3.45DD417 pKa = 3.83ATLSGTSGDD426 pKa = 3.67VTAHH430 pKa = 4.49YY431 pKa = 8.01TVKK434 pKa = 10.25VSHH437 pKa = 7.33DD438 pKa = 3.44AGTITGTEE446 pKa = 3.69VHH448 pKa = 5.81GTITVTNPNSVAVVADD464 pKa = 3.37IADD467 pKa = 4.0TFSDD471 pKa = 4.01GAVTCEE477 pKa = 3.7VDD479 pKa = 3.2GDD481 pKa = 4.29GTGVTVQPGDD491 pKa = 3.52TEE493 pKa = 4.67LSYY496 pKa = 11.1TCDD499 pKa = 3.79LPDD502 pKa = 3.94GSVPASDD509 pKa = 3.69LTNTVDD515 pKa = 4.44VTWADD520 pKa = 3.44QDD522 pKa = 4.04PAPLTVLDD530 pKa = 5.03AGNATDD536 pKa = 3.69TTDD539 pKa = 5.43AIDD542 pKa = 3.69FTEE545 pKa = 4.2NAVDD549 pKa = 3.59EE550 pKa = 4.85CADD553 pKa = 3.54VTDD556 pKa = 5.09TYY558 pKa = 11.48AGDD561 pKa = 4.16LGQVCVGDD569 pKa = 4.29ANPTTFDD576 pKa = 3.68YY577 pKa = 11.33SRR579 pKa = 11.84TWTLSNPGCATYY591 pKa = 11.13GNTATATAVDD601 pKa = 4.25SGTKK605 pKa = 10.26SSDD608 pKa = 3.43SQDD611 pKa = 3.35VQVCKK616 pKa = 8.92TTVDD620 pKa = 3.66TGAHH624 pKa = 6.22TIGFWTNNNGQALIKK639 pKa = 10.41AGPSTGGVCNAATTLRR655 pKa = 11.84TYY657 pKa = 11.45NPFKK661 pKa = 10.67DD662 pKa = 4.33LSATATCTQLAKK674 pKa = 10.67YY675 pKa = 9.16VADD678 pKa = 4.3VISKK682 pKa = 10.78AKK684 pKa = 10.43ASDD687 pKa = 3.39MTTMLKK693 pKa = 10.66AQMLGTALSVIHH705 pKa = 6.69LNPALANLNVDD716 pKa = 4.7LAHH719 pKa = 6.91MDD721 pKa = 3.59GKK723 pKa = 10.17DD724 pKa = 3.26VRR726 pKa = 11.84ASFGGASSMTVGNALTFVSNKK747 pKa = 10.01LVGSSWYY754 pKa = 10.35GGSKK758 pKa = 10.1VLQEE762 pKa = 4.04GAKK765 pKa = 10.5NLFDD769 pKa = 6.01AINNSRR775 pKa = 11.84GIYY778 pKa = 9.05TPP780 pKa = 3.7

Molecular weight: 80.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q8V7Z7|A0A0Q8V7Z7_9ACTN Cell division protein OS=Nocardioides sp. Root79 OX=1736600 GN=ASE19_13350 PE=4 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.93RR4 pKa = 11.84TYY6 pKa = 10.0QPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 4.97KK15 pKa = 10.04VHH17 pKa = 6.59GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSNRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.77GRR40 pKa = 11.84SSLAVV45 pKa = 3.16

Molecular weight: 5.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4673

0

4673

1534373

29

2215

328.3

35.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.352 ± 0.051

0.715 ± 0.008

6.573 ± 0.03

5.64 ± 0.04

2.829 ± 0.021

9.252 ± 0.032

2.1 ± 0.018

3.565 ± 0.027

2.093 ± 0.027

10.179 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.756 ± 0.016

1.864 ± 0.02

5.544 ± 0.029

2.752 ± 0.016

7.371 ± 0.036

5.195 ± 0.027

6.235 ± 0.039

9.479 ± 0.03

1.516 ± 0.016

1.987 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski