Saguaro cactus virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Alphacarmovirus

Average proteome isoelectric point is 8.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|P90338|P90338_9TOMB RNA-directed RNA polymerase OS=Saguaro cactus virus OX=52274 GN=SCVP57 PE=4 SV=1
MM1 pKa = 7.72GILSEE6 pKa = 4.4LLVGTTLTGGLVAVGLAGLQLRR28 pKa = 11.84IALKK32 pKa = 8.74TVEE35 pKa = 4.12VSVDD39 pKa = 3.65LVRR42 pKa = 11.84DD43 pKa = 3.58AYY45 pKa = 10.56EE46 pKa = 3.82FVVDD50 pKa = 3.8HH51 pKa = 6.54TKK53 pKa = 9.49TRR55 pKa = 11.84PPLPVRR61 pKa = 11.84EE62 pKa = 4.23HH63 pKa = 6.0VLSITRR69 pKa = 11.84EE70 pKa = 3.93DD71 pKa = 3.83DD72 pKa = 3.62PAPEE76 pKa = 5.32LEE78 pKa = 5.41LADD81 pKa = 4.19CLEE84 pKa = 4.49KK85 pKa = 10.54IEE87 pKa = 5.48DD88 pKa = 4.06KK89 pKa = 10.61EE90 pKa = 4.11TDD92 pKa = 3.4EE93 pKa = 4.21EE94 pKa = 5.05GKK96 pKa = 8.88TKK98 pKa = 10.08VVRR101 pKa = 11.84TRR103 pKa = 11.84TTNRR107 pKa = 11.84HH108 pKa = 4.61ARR110 pKa = 11.84GSFLRR115 pKa = 11.84RR116 pKa = 11.84LIAEE120 pKa = 4.81AKK122 pKa = 9.08CHH124 pKa = 6.53FGGTPTATRR133 pKa = 11.84ANEE136 pKa = 3.67LAAMKK141 pKa = 10.02FLVGKK146 pKa = 10.27CRR148 pKa = 11.84DD149 pKa = 3.08HH150 pKa = 7.45HH151 pKa = 6.95LVVLHH156 pKa = 5.78TKK158 pKa = 7.78EE159 pKa = 3.96VCHH162 pKa = 6.28AAMVGLFTPDD172 pKa = 3.54DD173 pKa = 4.13DD174 pKa = 5.64EE175 pKa = 5.1IAAMAQLNSHH185 pKa = 6.83AAYY188 pKa = 8.99VARR191 pKa = 11.84VKK193 pKa = 10.52LHH195 pKa = 5.97SAVTVDD201 pKa = 3.33PWWVKK206 pKa = 9.79ILRR209 pKa = 11.84SGEE212 pKa = 4.08RR213 pKa = 11.84TAGWQQLWWVLWGAPAQRR231 pKa = 11.84GFTYY235 pKa = 10.41HH236 pKa = 7.22KK237 pKa = 10.8

Molecular weight: 26.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|P89112|P89112_9TOMB Isoform of P89111 Uncharacterized protein SCVCP OS=Saguaro cactus virus OX=52274 GN=SCVCP PE=4 SV=1
MM1 pKa = 7.12GRR3 pKa = 11.84IRR5 pKa = 11.84AWLLLLLLVQLGQCVFLIGVGLRR28 pKa = 11.84SPRR31 pKa = 11.84NRR33 pKa = 11.84SAPLDD38 pKa = 3.88LCWGCRR44 pKa = 11.84ISLMLSCLYY53 pKa = 10.85DD54 pKa = 3.63LVLLLVSPGPLGRR67 pKa = 11.84VPGLRR72 pKa = 11.84GNPP75 pKa = 3.43

Molecular weight: 8.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

2

8

2104

53

755

263.0

29.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.175 ± 0.673

2.519 ± 0.237

5.276 ± 0.329

4.943 ± 0.832

3.612 ± 0.333

6.749 ± 0.266

2.804 ± 0.51

4.8 ± 0.393

4.99 ± 0.414

10.029 ± 1.158

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.901 ± 0.259

3.089 ± 0.338

4.753 ± 0.43

3.327 ± 0.328

7.557 ± 0.539

5.466 ± 1.115

5.941 ± 1.079

8.745 ± 0.432

2.139 ± 0.099

3.089 ± 0.374
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski