Monosporascus sp. GIB2

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Xylariomycetidae; Xylariales; Xylariales incertae sedis; Monosporascus; unclassified Monosporascus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11773 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q4TPP1|A0A4Q4TPP1_9PEZI Uncharacterized protein OS=Monosporascus sp. GIB2 OX=2211647 GN=DL765_009128 PE=4 SV=1
MM1 pKa = 7.1NLCMAIAAPRR11 pKa = 11.84SQDD14 pKa = 3.08GFFSLPASRR23 pKa = 11.84ISKK26 pKa = 9.68PLTVLKK32 pKa = 10.33RR33 pKa = 11.84QEE35 pKa = 4.15DD36 pKa = 4.28TDD38 pKa = 3.84VPLYY42 pKa = 10.65DD43 pKa = 3.55VSEE46 pKa = 4.07VSYY49 pKa = 10.81LIEE52 pKa = 4.63LSIGTPGQSVKK63 pKa = 10.64VAIDD67 pKa = 3.45TGSSEE72 pKa = 4.36LWVDD76 pKa = 3.91PVCQNSQSLSEE87 pKa = 4.08VDD89 pKa = 3.2EE90 pKa = 5.26CIANGIYY97 pKa = 10.41DD98 pKa = 4.39PSQSTTFEE106 pKa = 4.63DD107 pKa = 3.97VNSTNTIPYY116 pKa = 8.48GIGVVRR122 pKa = 11.84IEE124 pKa = 3.94YY125 pKa = 10.39VRR127 pKa = 11.84DD128 pKa = 3.23NVAFPNSTINLTDD141 pKa = 3.25VQFGVATASRR151 pKa = 11.84QLNEE155 pKa = 4.58GIMGLSFGGGTPEE168 pKa = 5.07ADD170 pKa = 2.85LRR172 pKa = 11.84YY173 pKa = 10.62NNIVDD178 pKa = 3.99EE179 pKa = 5.43LYY181 pKa = 10.69LQNVTQSRR189 pKa = 11.84AFSVALGSSDD199 pKa = 3.6SDD201 pKa = 3.46NDD203 pKa = 3.72SVVIFGGIDD212 pKa = 3.13TSKK215 pKa = 10.49YY216 pKa = 10.15AGPLRR221 pKa = 11.84TLPILGPQNGEE232 pKa = 3.74TLNRR236 pKa = 11.84YY237 pKa = 7.23WVQLDD242 pKa = 4.19SISTTVDD249 pKa = 3.43GSSHH253 pKa = 6.46TYY255 pKa = 10.38DD256 pKa = 3.31SSSLPVVLDD265 pKa = 3.52TGSTFCSLPPAVVNGMMEE283 pKa = 4.22DD284 pKa = 4.0LGGQINNQGQVLVDD298 pKa = 4.02CSLVDD303 pKa = 3.75NEE305 pKa = 4.66GTFDD309 pKa = 4.06FDD311 pKa = 4.47FGNITIRR318 pKa = 11.84IPYY321 pKa = 8.46SQFVIQANSEE331 pKa = 4.39VCVLGATPDD340 pKa = 3.67DD341 pKa = 4.25QIALLGDD348 pKa = 3.49SFLRR352 pKa = 11.84SAYY355 pKa = 10.54VVFDD359 pKa = 3.77QNNMEE364 pKa = 4.36VAMAPYY370 pKa = 9.87ANCGQTEE377 pKa = 3.71QRR379 pKa = 11.84IPDD382 pKa = 3.87GGVSGVKK389 pKa = 10.04GAEE392 pKa = 3.86QTATDD397 pKa = 3.95IDD399 pKa = 4.16GDD401 pKa = 4.41DD402 pKa = 3.53NVMLGG407 pKa = 3.53

Molecular weight: 43.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q4TZB6|A0A4Q4TZB6_9PEZI Uncharacterized protein OS=Monosporascus sp. GIB2 OX=2211647 GN=DL765_007306 PE=4 SV=1
MM1 pKa = 7.61ASAGAWEE8 pKa = 4.62ARR10 pKa = 11.84TPPLAATSAMVLTAMLSEE28 pKa = 4.41SAMSVTGAVNATGAISTIIRR48 pKa = 11.84CGGYY52 pKa = 9.38SGEE55 pKa = 4.36VGCVPRR61 pKa = 11.84GAAVDD66 pKa = 4.04LGSAGRR72 pKa = 11.84IDD74 pKa = 3.18ITGAVIVPSVLLLLPPANKK93 pKa = 10.01SVGHH97 pKa = 5.23VRR99 pKa = 11.84GPQPDD104 pKa = 3.72VTHH107 pKa = 6.79KK108 pKa = 10.86LVLVSIASKK117 pKa = 9.9TPMAKK122 pKa = 9.47PARR125 pKa = 11.84RR126 pKa = 11.84RR127 pKa = 11.84TWSMMKK133 pKa = 9.91AQSDD137 pKa = 3.95AAPVRR142 pKa = 11.84LDD144 pKa = 3.75DD145 pKa = 4.16NVGKK149 pKa = 10.5GLEE152 pKa = 4.21PLHH155 pKa = 6.01HH156 pKa = 6.78HH157 pKa = 7.1RR158 pKa = 11.84LPVAYY163 pKa = 9.55FSALIDD169 pKa = 3.28RR170 pKa = 11.84LFARR174 pKa = 11.84RR175 pKa = 11.84RR176 pKa = 11.84CGMLCDD182 pKa = 4.13SRR184 pKa = 11.84FAMHH188 pKa = 7.05LSASLSSNPNALLHH202 pKa = 6.14EE203 pKa = 4.4MRR205 pKa = 11.84QRR207 pKa = 11.84HH208 pKa = 5.74AAGQPVGCSSTRR220 pKa = 11.84HH221 pKa = 4.91FGRR224 pKa = 11.84RR225 pKa = 11.84YY226 pKa = 9.83GLLVNDD232 pKa = 4.83RR233 pKa = 11.84AAMAALYY240 pKa = 10.15HH241 pKa = 6.11SRR243 pKa = 3.83

Molecular weight: 25.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11773

0

11773

5809346

66

14987

493.4

54.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.278 ± 0.021

1.189 ± 0.009

5.905 ± 0.015

6.318 ± 0.023

3.532 ± 0.012

7.344 ± 0.023

2.339 ± 0.009

4.522 ± 0.016

4.572 ± 0.021

8.761 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.097 ± 0.008

3.487 ± 0.012

6.211 ± 0.023

3.854 ± 0.015

6.627 ± 0.02

7.856 ± 0.021

5.733 ± 0.016

6.198 ± 0.018

1.469 ± 0.007

2.709 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski