Amino acid dipepetide frequency for Monosporascus sp. GIB2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.959AlaAla: 10.959 ± 0.064	1.099AlaCys: 1.099 ± 0.015	4.868AlaAsp: 4.868 ± 0.033	5.793AlaGlu: 5.793 ± 0.044	3.166AlaPhe: 3.166 ± 0.026	6.673AlaGly: 6.673 ± 0.045	1.878AlaHis: 1.878 ± 0.018	4.14AlaIle: 4.14 ± 0.033	4.138AlaLys: 4.138 ± 0.034	8.109AlaLeu: 8.109 ± 0.047	2.042AlaMet: 2.042 ± 0.021	3.032AlaAsn: 3.032 ± 0.023	5.221AlaPro: 5.221 ± 0.046	3.438AlaGln: 3.438 ± 0.031	5.797AlaArg: 5.797 ± 0.035	7.435AlaSer: 7.435 ± 0.041	5.54AlaThr: 5.54 ± 0.033	5.936AlaVal: 5.936 ± 0.036	1.226AlaTrp: 1.226 ± 0.02	2.288AlaTyr: 2.288 ± 0.019	0.0AlaXaa: 0.0 ± 0.0
Cys	0.972CysAla: 0.972 ± 0.015	0.224CysCys: 0.224 ± 0.007	0.671CysAsp: 0.671 ± 0.011	0.605CysGlu: 0.605 ± 0.011	0.515CysPhe: 0.515 ± 0.012	0.967CysGly: 0.967 ± 0.015	0.316CysHis: 0.316 ± 0.008	0.651CysIle: 0.651 ± 0.01	0.471CysLys: 0.471 ± 0.009	1.169CysLeu: 1.169 ± 0.013	0.243CysMet: 0.243 ± 0.007	0.409CysAsn: 0.409 ± 0.009	0.66CysPro: 0.66 ± 0.013	0.401CysGln: 0.401 ± 0.008	0.793CysArg: 0.793 ± 0.013	0.843CysSer: 0.843 ± 0.012	0.64CysThr: 0.64 ± 0.012	0.785CysVal: 0.785 ± 0.012	0.205CysTrp: 0.205 ± 0.006	0.347CysTyr: 0.347 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	5.343AspAla: 5.343 ± 0.033	0.639AspCys: 0.639 ± 0.013	4.599AspAsp: 4.599 ± 0.046	4.652AspGlu: 4.652 ± 0.035	2.194AspPhe: 2.194 ± 0.021	4.663AspGly: 4.663 ± 0.034	1.226AspHis: 1.226 ± 0.015	2.995AspIle: 2.995 ± 0.023	2.333AspLys: 2.333 ± 0.023	5.054AspLeu: 5.054 ± 0.034	1.307AspMet: 1.307 ± 0.014	1.876AspAsn: 1.876 ± 0.016	3.615AspPro: 3.615 ± 0.028	1.818AspGln: 1.818 ± 0.02	3.364AspArg: 3.364 ± 0.03	4.034AspSer: 4.034 ± 0.027	2.847AspThr: 2.847 ± 0.022	3.893AspVal: 3.893 ± 0.03	0.926AspTrp: 0.926 ± 0.013	1.67AspTyr: 1.67 ± 0.017	0.0AspXaa: 0.0 ± 0.0
Glu	5.995GluAla: 5.995 ± 0.045	0.655GluCys: 0.655 ± 0.012	4.342GluAsp: 4.342 ± 0.03	5.471GluGlu: 5.471 ± 0.053	1.972GluPhe: 1.972 ± 0.019	4.104GluGly: 4.104 ± 0.028	1.404GluHis: 1.404 ± 0.016	2.914GluIle: 2.914 ± 0.025	3.541GluLys: 3.541 ± 0.035	5.422GluLeu: 5.422 ± 0.034	1.407GluMet: 1.407 ± 0.017	2.236GluAsn: 2.236 ± 0.02	3.061GluPro: 3.061 ± 0.039	2.455GluGln: 2.455 ± 0.025	4.277GluArg: 4.277 ± 0.034	4.151GluSer: 4.151 ± 0.032	3.485GluThr: 3.485 ± 0.031	3.725GluVal: 3.725 ± 0.027	0.899GluTrp: 0.899 ± 0.013	1.659GluTyr: 1.659 ± 0.016	0.0GluXaa: 0.0 ± 0.0
Phe	3.042PheAla: 3.042 ± 0.026	0.517PheCys: 0.517 ± 0.01	2.236PheAsp: 2.236 ± 0.022	2.198PheGlu: 2.198 ± 0.02	1.462PhePhe: 1.462 ± 0.017	2.836PheGly: 2.836 ± 0.022	0.826PheHis: 0.826 ± 0.013	1.576PheIle: 1.576 ± 0.019	1.439PheLys: 1.439 ± 0.017	3.196PheLeu: 3.196 ± 0.029	0.735PheMet: 0.735 ± 0.011	1.296PheAsn: 1.296 ± 0.015	1.854PhePro: 1.854 ± 0.021	1.277PheGln: 1.277 ± 0.015	2.1PheArg: 2.1 ± 0.022	2.714PheSer: 2.714 ± 0.025	1.988PheThr: 1.988 ± 0.018	2.359PheVal: 2.359 ± 0.024	0.62PheTrp: 0.62 ± 0.011	1.052PheTyr: 1.052 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	6.261GlyAla: 6.261 ± 0.042	0.895GlyCys: 0.895 ± 0.014	4.272GlyAsp: 4.272 ± 0.032	4.021GlyGlu: 4.021 ± 0.032	2.75GlyPhe: 2.75 ± 0.024	6.917GlyGly: 6.917 ± 0.065	1.837GlyHis: 1.837 ± 0.022	3.431GlyIle: 3.431 ± 0.028	3.418GlyLys: 3.418 ± 0.027	6.143GlyLeu: 6.143 ± 0.033	1.558GlyMet: 1.558 ± 0.018	2.651GlyAsn: 2.651 ± 0.026	3.786GlyPro: 3.786 ± 0.03	2.569GlyGln: 2.569 ± 0.02	4.897GlyArg: 4.897 ± 0.029	5.944GlySer: 5.944 ± 0.04	4.132GlyThr: 4.132 ± 0.026	4.604GlyVal: 4.604 ± 0.032	1.196GlyTrp: 1.196 ± 0.016	2.162GlyTyr: 2.162 ± 0.024	0.0GlyXaa: 0.0 ± 0.0
His	1.918HisAla: 1.918 ± 0.02	0.301HisCys: 0.301 ± 0.008	1.317HisAsp: 1.317 ± 0.018	1.347HisGlu: 1.347 ± 0.015	0.858HisPhe: 0.858 ± 0.013	1.824HisGly: 1.824 ± 0.018	0.889HisHis: 0.889 ± 0.018	1.135HisIle: 1.135 ± 0.016	0.863HisLys: 0.863 ± 0.013	2.127HisLeu: 2.127 ± 0.023	0.495HisMet: 0.495 ± 0.009	0.808HisAsn: 0.808 ± 0.012	1.642HisPro: 1.642 ± 0.019	0.976HisGln: 0.976 ± 0.014	1.61HisArg: 1.61 ± 0.019	1.663HisSer: 1.663 ± 0.018	1.151HisThr: 1.151 ± 0.014	1.454HisVal: 1.454 ± 0.017	0.333HisTrp: 0.333 ± 0.008	0.679HisTyr: 0.679 ± 0.012	0.0HisXaa: 0.0 ± 0.0
Ile	4.005IleAla: 4.005 ± 0.03	0.692IleCys: 0.692 ± 0.012	2.742IleAsp: 2.742 ± 0.021	2.76IleGlu: 2.76 ± 0.021	1.74IlePhe: 1.74 ± 0.017	2.998IleGly: 2.998 ± 0.024	1.084IleHis: 1.084 ± 0.014	2.164IleIle: 2.164 ± 0.023	1.987IleLys: 1.987 ± 0.021	4.14IleLeu: 4.14 ± 0.028	0.946IleMet: 0.946 ± 0.013	1.614IleAsn: 1.614 ± 0.018	2.804IlePro: 2.804 ± 0.026	1.742IleGln: 1.742 ± 0.022	2.879IleArg: 2.879 ± 0.022	3.37IleSer: 3.37 ± 0.027	2.613IleThr: 2.613 ± 0.022	2.961IleVal: 2.961 ± 0.022	0.685IleTrp: 0.685 ± 0.013	1.291IleTyr: 1.291 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	4.279LysAla: 4.279 ± 0.032	0.434LysCys: 0.434 ± 0.009	2.663LysAsp: 2.663 ± 0.021	3.16LysGlu: 3.16 ± 0.031	1.37LysPhe: 1.37 ± 0.017	3.022LysGly: 3.022 ± 0.029	1.033LysHis: 1.033 ± 0.014	2.018LysIle: 2.018 ± 0.019	3.056LysLys: 3.056 ± 0.037	3.914LysLeu: 3.914 ± 0.028	0.929LysMet: 0.929 ± 0.014	1.551LysAsn: 1.551 ± 0.016	2.735LysPro: 2.735 ± 0.03	1.754LysGln: 1.754 ± 0.022	3.312LysArg: 3.312 ± 0.027	3.232LysSer: 3.232 ± 0.024	2.647LysThr: 2.647 ± 0.024	2.719LysVal: 2.719 ± 0.022	0.633LysTrp: 0.633 ± 0.011	1.262LysTyr: 1.262 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	8.247LeuAla: 8.247 ± 0.046	1.171LeuCys: 1.171 ± 0.014	5.255LeuAsp: 5.255 ± 0.031	5.626LeuGlu: 5.626 ± 0.047	3.079LeuPhe: 3.079 ± 0.027	6.117LeuGly: 6.117 ± 0.038	2.082LeuHis: 2.082 ± 0.021	3.53LeuIle: 3.53 ± 0.028	3.976LeuLys: 3.976 ± 0.029	8.265LeuLeu: 8.265 ± 0.055	1.737LeuMet: 1.737 ± 0.017	2.896LeuAsn: 2.896 ± 0.021	5.389LeuPro: 5.389 ± 0.035	3.616LeuGln: 3.616 ± 0.029	6.285LeuArg: 6.285 ± 0.038	6.87LeuSer: 6.87 ± 0.043	4.523LeuThr: 4.523 ± 0.036	5.489LeuVal: 5.489 ± 0.034	1.249LeuTrp: 1.249 ± 0.015	2.209LeuTyr: 2.209 ± 0.019	0.0LeuXaa: 0.0 ± 0.0
Met	2.298MetAla: 2.298 ± 0.022	0.23MetCys: 0.23 ± 0.006	1.227MetAsp: 1.227 ± 0.014	1.253MetGlu: 1.253 ± 0.015	0.679MetPhe: 0.679 ± 0.012	1.42MetGly: 1.42 ± 0.018	0.463MetHis: 0.463 ± 0.008	0.896MetIle: 0.896 ± 0.012	0.938MetLys: 0.938 ± 0.015	1.796MetLeu: 1.796 ± 0.018	0.539MetMet: 0.539 ± 0.011	0.743MetAsn: 0.743 ± 0.011	1.281MetPro: 1.281 ± 0.016	0.806MetGln: 0.806 ± 0.014	1.366MetArg: 1.366 ± 0.015	1.708MetSer: 1.708 ± 0.016	1.27MetThr: 1.27 ± 0.014	1.303MetVal: 1.303 ± 0.017	0.262MetTrp: 0.262 ± 0.006	0.496MetTyr: 0.496 ± 0.009	0.0MetXaa: 0.0 ± 0.0
Asn	3.148AsnAla: 3.148 ± 0.027	0.417AsnCys: 0.417 ± 0.009	1.871AsnAsp: 1.871 ± 0.018	1.91AsnGlu: 1.91 ± 0.02	1.267AsnPhe: 1.267 ± 0.015	3.042AsnGly: 3.042 ± 0.029	0.802AsnHis: 0.802 ± 0.011	1.83AsnIle: 1.83 ± 0.02	1.436AsnLys: 1.436 ± 0.016	3.047AsnLeu: 3.047 ± 0.023	0.771AsnMet: 0.771 ± 0.014	1.446AsnAsn: 1.446 ± 0.02	2.423AsnPro: 2.423 ± 0.023	1.214AsnGln: 1.214 ± 0.016	2.002AsnArg: 2.002 ± 0.018	2.573AsnSer: 2.573 ± 0.022	1.971AsnThr: 1.971 ± 0.018	2.168AsnVal: 2.168 ± 0.021	0.539AsnTrp: 0.539 ± 0.009	0.989AsnTyr: 0.989 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	5.686ProAla: 5.686 ± 0.043	0.519ProCys: 0.519 ± 0.01	3.507ProAsp: 3.507 ± 0.027	4.125ProGlu: 4.125 ± 0.032	1.969ProPhe: 1.969 ± 0.021	4.519ProGly: 4.519 ± 0.036	1.327ProHis: 1.327 ± 0.016	2.298ProIle: 2.298 ± 0.021	2.6ProLys: 2.6 ± 0.023	4.812ProLeu: 4.812 ± 0.034	1.073ProMet: 1.073 ± 0.014	2.155ProAsn: 2.155 ± 0.02	5.585ProPro: 5.585 ± 0.067	2.475ProGln: 2.475 ± 0.025	3.853ProArg: 3.853 ± 0.032	5.793ProSer: 5.793 ± 0.041	3.935ProThr: 3.935 ± 0.039	3.583ProVal: 3.583 ± 0.03	0.774ProTrp: 0.774 ± 0.012	1.521ProTyr: 1.521 ± 0.018	0.0ProXaa: 0.0 ± 0.0
Gln	3.51GlnAla: 3.51 ± 0.03	0.407GlnCys: 0.407 ± 0.01	2.019GlnAsp: 2.019 ± 0.021	2.326GlnGlu: 2.326 ± 0.024	1.218GlnPhe: 1.218 ± 0.018	2.441GlnGly: 2.441 ± 0.02	1.051GlnHis: 1.051 ± 0.017	1.687GlnIle: 1.687 ± 0.017	1.829GlnLys: 1.829 ± 0.022	3.341GlnLeu: 3.341 ± 0.031	0.808GlnMet: 0.808 ± 0.013	1.379GlnAsn: 1.379 ± 0.016	2.616GlnPro: 2.616 ± 0.028	2.516GlnGln: 2.516 ± 0.042	2.692GlnArg: 2.692 ± 0.026	2.834GlnSer: 2.834 ± 0.026	2.106GlnThr: 2.106 ± 0.021	2.103GlnVal: 2.103 ± 0.022	0.548GlnTrp: 0.548 ± 0.009	1.105GlnTyr: 1.105 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	5.53ArgAla: 5.53 ± 0.039	0.753ArgCys: 0.753 ± 0.012	3.989ArgAsp: 3.989 ± 0.035	4.252ArgGlu: 4.252 ± 0.028	2.214ArgPhe: 2.214 ± 0.018	4.456ArgGly: 4.456 ± 0.033	1.735ArgHis: 1.735 ± 0.017	2.926ArgIle: 2.926 ± 0.023	3.332ArgLys: 3.332 ± 0.026	5.972ArgLeu: 5.972 ± 0.038	1.376ArgMet: 1.376 ± 0.016	2.377ArgAsn: 2.377 ± 0.02	3.923ArgPro: 3.923 ± 0.033	2.75ArgGln: 2.75 ± 0.025	5.928ArgArg: 5.928 ± 0.049	4.863ArgSer: 4.863 ± 0.038	3.444ArgThr: 3.444 ± 0.027	3.681ArgVal: 3.681 ± 0.026	1.013ArgTrp: 1.013 ± 0.016	1.757ArgTyr: 1.757 ± 0.019	0.0ArgXaa: 0.0 ± 0.0
Ser	6.846SerAla: 6.846 ± 0.039	0.816SerCys: 0.816 ± 0.012	4.115SerAsp: 4.115 ± 0.031	4.111SerGlu: 4.111 ± 0.031	2.776SerPhe: 2.776 ± 0.027	5.854SerGly: 5.854 ± 0.037	1.795SerHis: 1.795 ± 0.021	3.487SerIle: 3.487 ± 0.023	3.374SerLys: 3.374 ± 0.028	6.684SerLeu: 6.684 ± 0.041	1.61SerMet: 1.61 ± 0.017	2.703SerAsn: 2.703 ± 0.026	5.417SerPro: 5.417 ± 0.044	3.014SerGln: 3.014 ± 0.027	5.207SerArg: 5.207 ± 0.041	8.181SerSer: 8.181 ± 0.062	5.051SerThr: 5.051 ± 0.036	4.398SerVal: 4.398 ± 0.032	1.121SerTrp: 1.121 ± 0.016	1.998SerTyr: 1.998 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	5.452ThrAla: 5.452 ± 0.034	0.714ThrCys: 0.714 ± 0.011	2.772ThrAsp: 2.772 ± 0.023	3.082ThrGlu: 3.082 ± 0.027	2.052ThrPhe: 2.052 ± 0.019	4.266ThrGly: 4.266 ± 0.032	1.172ThrHis: 1.172 ± 0.013	2.779ThrIle: 2.779 ± 0.027	2.41ThrLys: 2.41 ± 0.025	4.858ThrLeu: 4.858 ± 0.033	1.148ThrMet: 1.148 ± 0.014	1.905ThrAsn: 1.905 ± 0.017	4.276ThrPro: 4.276 ± 0.038	1.908ThrGln: 1.908 ± 0.021	3.298ThrArg: 3.298 ± 0.025	4.98ThrSer: 4.98 ± 0.038	4.136ThrThr: 4.136 ± 0.044	3.729ThrVal: 3.729 ± 0.032	0.848ThrTrp: 0.848 ± 0.012	1.548ThrTyr: 1.548 ± 0.02	0.0ThrXaa: 0.0 ± 0.0
Val	5.762ValAla: 5.762 ± 0.036	0.799ValCys: 0.799 ± 0.013	3.883ValAsp: 3.883 ± 0.026	4.008ValGlu: 4.008 ± 0.03	2.378ValPhe: 2.378 ± 0.025	4.186ValGly: 4.186 ± 0.03	1.375ValHis: 1.375 ± 0.014	2.784ValIle: 2.784 ± 0.021	2.804ValLys: 2.804 ± 0.026	5.653ValLeu: 5.653 ± 0.037	1.291ValMet: 1.291 ± 0.014	2.159ValAsn: 2.159 ± 0.02	3.708ValPro: 3.708 ± 0.031	2.257ValGln: 2.257 ± 0.022	3.851ValArg: 3.851 ± 0.026	4.47ValSer: 4.47 ± 0.027	3.512ValThr: 3.512 ± 0.027	4.496ValVal: 4.496 ± 0.035	0.886ValTrp: 0.886 ± 0.013	1.719ValTyr: 1.719 ± 0.018	0.0ValXaa: 0.0 ± 0.0
Trp	1.272TrpAla: 1.272 ± 0.019	0.192TrpCys: 0.192 ± 0.005	0.986TrpAsp: 0.986 ± 0.014	0.886TrpGlu: 0.886 ± 0.014	0.545TrpPhe: 0.545 ± 0.009	0.975TrpGly: 0.975 ± 0.014	0.352TrpHis: 0.352 ± 0.008	0.731TrpIle: 0.731 ± 0.012	0.752TrpLys: 0.752 ± 0.012	1.354TrpLeu: 1.354 ± 0.016	0.349TrpMet: 0.349 ± 0.007	0.604TrpAsn: 0.604 ± 0.01	0.623TrpPro: 0.623 ± 0.012	0.54TrpGln: 0.54 ± 0.01	1.045TrpArg: 1.045 ± 0.015	0.983TrpSer: 0.983 ± 0.013	0.883TrpThr: 0.883 ± 0.013	0.926TrpVal: 0.926 ± 0.012	0.274TrpTrp: 0.274 ± 0.008	0.415TrpTyr: 0.415 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.251TyrAla: 2.251 ± 0.023	0.412TyrCys: 0.412 ± 0.009	1.715TyrAsp: 1.715 ± 0.018	1.594TyrGlu: 1.594 ± 0.016	1.12TyrPhe: 1.12 ± 0.014	2.165TyrGly: 2.165 ± 0.023	0.742TyrHis: 0.742 ± 0.012	1.244TyrIle: 1.244 ± 0.016	1.026TyrLys: 1.026 ± 0.014	2.558TyrLeu: 2.558 ± 0.023	0.607TyrMet: 0.607 ± 0.01	1.027TyrAsn: 1.027 ± 0.014	1.489TyrPro: 1.489 ± 0.017	1.012TyrGln: 1.012 ± 0.014	1.714TyrArg: 1.714 ± 0.018	1.914TyrSer: 1.914 ± 0.02	1.461TyrThr: 1.461 ± 0.018	1.667TyrVal: 1.667 ± 0.018	0.448TyrTrp: 0.448 ± 0.008	0.921TyrTyr: 0.921 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.006XaaXaa: 0.006 ± 0.005

Statistics based on 11773 proteins (5809346 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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