Fusobacterium mortiferum ATCC 9817
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2532 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C3WFT2|C3WFT2_FUSMR 30S ribosomal protein S6 OS=Fusobacterium mortiferum ATCC 9817 OX=469616 GN=rpsF PE=3 SV=1
MM1 pKa = 7.59 KK2 pKa = 10.23 KK3 pKa = 10.36 YY4 pKa = 9.22 ICKK7 pKa = 9.46 VCGYY11 pKa = 9.51 EE12 pKa = 3.84 YY13 pKa = 10.89 DD14 pKa = 3.92 PAVGDD19 pKa = 3.55 VDD21 pKa = 3.84 NGVAAGTAWEE31 pKa = 4.84 DD32 pKa = 3.73 VPEE35 pKa = 4.74 DD36 pKa = 4.08 WLCPLCGVGKK46 pKa = 10.4 DD47 pKa = 3.48 EE48 pKa = 5.58 FEE50 pKa = 4.12 PAEE53 pKa = 4.01
Molecular weight: 5.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.84
IPC2_protein 3.961
IPC_protein 3.834
Toseland 3.643
ProMoST 3.935
Dawson 3.821
Bjellqvist 4.062
Wikipedia 3.757
Rodwell 3.668
Grimsley 3.567
Solomon 3.795
Lehninger 3.745
Nozaki 3.948
DTASelect 4.113
Thurlkill 3.706
EMBOSS 3.757
Sillero 3.948
Patrickios 0.401
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.94
Protein with the highest isoelectric point:
>tr|C3WBM1|C3WBM1_FUSMR Ribosomal protein S12 methylthiotransferase RimO OS=Fusobacterium mortiferum ATCC 9817 OX=469616 GN=rimO PE=3 SV=1
MM1 pKa = 7.77 IIKK4 pKa = 10.26 KK5 pKa = 9.73 ILSFLFFIIFFIGCSSIGIDD25 pKa = 3.56 KK26 pKa = 10.88 NEE28 pKa = 4.61 KK29 pKa = 7.42 ISKK32 pKa = 8.47 YY33 pKa = 8.38 FTMGEE38 pKa = 3.86 ATYY41 pKa = 10.81 SSTAIRR47 pKa = 11.84 RR48 pKa = 11.84 GIKK51 pKa = 8.22 NTPSWSEE58 pKa = 3.77 RR59 pKa = 11.84 TNIRR63 pKa = 11.84 YY64 pKa = 6.62 TARR67 pKa = 11.84 RR68 pKa = 11.84 LDD70 pKa = 3.71 EE71 pKa = 3.81 VRR73 pKa = 11.84 EE74 pKa = 3.92 ILGRR78 pKa = 11.84 PVVVSSWFRR87 pKa = 11.84 SARR90 pKa = 11.84 LNRR93 pKa = 11.84 AVGGSSSSGHH103 pKa = 5.74 RR104 pKa = 11.84 KK105 pKa = 9.42 GMAVDD110 pKa = 4.57 IILKK114 pKa = 10.16 KK115 pKa = 10.71 GSAGKK120 pKa = 10.17 KK121 pKa = 8.03 EE122 pKa = 3.85 YY123 pKa = 10.67 EE124 pKa = 3.9 RR125 pKa = 11.84 VKK127 pKa = 11.05 NRR129 pKa = 11.84 LEE131 pKa = 4.43 SFDD134 pKa = 3.44 QLIYY138 pKa = 10.04 YY139 pKa = 7.71 PRR141 pKa = 11.84 RR142 pKa = 11.84 GHH144 pKa = 5.81 LHH146 pKa = 6.53 IGFKK150 pKa = 9.74 QYY152 pKa = 10.66 RR153 pKa = 11.84 FQEE156 pKa = 4.35 RR157 pKa = 11.84 KK158 pKa = 8.52 QVMVRR163 pKa = 11.84 YY164 pKa = 9.05 NN165 pKa = 2.95
Molecular weight: 19.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 9.911
IPC_protein 10.672
Toseland 10.774
ProMoST 10.452
Dawson 10.877
Bjellqvist 10.57
Wikipedia 11.082
Rodwell 11.169
Grimsley 10.921
Solomon 10.965
Lehninger 10.935
Nozaki 10.745
DTASelect 10.57
Thurlkill 10.774
EMBOSS 11.169
Sillero 10.804
Patrickios 10.877
IPC_peptide 10.979
IPC2_peptide 9.414
IPC2.peptide.svr19 8.467
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2532
0
2532
778824
30
1602
307.6
34.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.456 ± 0.059
0.941 ± 0.02
5.127 ± 0.041
8.386 ± 0.067
4.75 ± 0.04
6.83 ± 0.049
1.234 ± 0.019
9.759 ± 0.052
9.485 ± 0.057
9.35 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.572 ± 0.022
5.741 ± 0.051
2.779 ± 0.029
2.208 ± 0.02
3.585 ± 0.036
5.653 ± 0.037
4.826 ± 0.035
6.341 ± 0.042
0.691 ± 0.014
4.286 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here