Acinetobacter indicus CIP 110367
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2967 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V2VME2|V2VME2_9GAMM Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C OS=Acinetobacter indicus CIP 110367 OX=1341679 GN=gatC PE=3 SV=1
MM1 pKa = 7.7 KK2 pKa = 10.11 KK3 pKa = 9.69 YY4 pKa = 10.65 QCIVCGWIYY13 pKa = 11.09 DD14 pKa = 4.04 EE15 pKa = 5.55 AEE17 pKa = 3.9 GWPQDD22 pKa = 3.98 GIVAGTKK29 pKa = 9.43 WEE31 pKa = 5.47 DD32 pKa = 3.71 IPDD35 pKa = 3.75 DD36 pKa = 4.13 WTCPDD41 pKa = 4.02 CGVSKK46 pKa = 11.14 VDD48 pKa = 3.99 FEE50 pKa = 4.42 MVEE53 pKa = 3.84 II54 pKa = 4.69
Molecular weight: 6.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.76
IPC2_protein 3.884
IPC_protein 3.783
Toseland 3.592
ProMoST 3.973
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.745
Rodwell 3.63
Grimsley 3.503
Solomon 3.757
Lehninger 3.719
Nozaki 3.923
DTASelect 4.113
Thurlkill 3.668
EMBOSS 3.745
Sillero 3.91
Patrickios 0.693
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.821
Protein with the highest isoelectric point:
>tr|V2U6Y2|V2U6Y2_9GAMM Resolvase/invertase-type recombinase catalytic domain-containing protein OS=Acinetobacter indicus CIP 110367 OX=1341679 GN=P253_03044 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.55 RR3 pKa = 11.84 TFQPSEE9 pKa = 3.97 LKK11 pKa = 10.13 RR12 pKa = 11.84 KK13 pKa = 8.98 RR14 pKa = 11.84 VHH16 pKa = 6.36 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 AGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.18 GRR39 pKa = 11.84 HH40 pKa = 5.1 SLTVV44 pKa = 3.06
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.402
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2967
0
2967
905149
27
1526
305.1
34.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.088 ± 0.053
0.976 ± 0.015
5.125 ± 0.036
5.968 ± 0.039
4.135 ± 0.035
6.454 ± 0.042
2.488 ± 0.028
6.452 ± 0.038
5.182 ± 0.039
10.745 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.56 ± 0.021
4.057 ± 0.037
4.114 ± 0.03
5.918 ± 0.062
4.709 ± 0.037
5.906 ± 0.031
5.044 ± 0.033
6.603 ± 0.038
1.272 ± 0.019
3.204 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here