Dysgonamonadaceae bacterium

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Dysgonomonadaceae; unclassified Dysgonamonadaceae

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2317 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N4SBL9|A0A2N4SBL9_9BACT Serine hydroxymethyltransferase OS=Dysgonamonadaceae bacterium OX=2065189 GN=glyA PE=3 SV=1
MM1 pKa = 7.74KK2 pKa = 9.92IDD4 pKa = 3.4TNKK7 pKa = 10.35YY8 pKa = 9.1VVLSYY13 pKa = 11.07DD14 pKa = 3.64LNVGEE19 pKa = 4.68EE20 pKa = 4.47GEE22 pKa = 4.16EE23 pKa = 4.2LEE25 pKa = 5.55LMEE28 pKa = 5.18CATAEE33 pKa = 4.23KK34 pKa = 9.89PLEE37 pKa = 4.55FIFGTNSMLPAFEE50 pKa = 4.93KK51 pKa = 10.9NLEE54 pKa = 4.18GLDD57 pKa = 3.64QGDD60 pKa = 3.55NFQFTLTSDD69 pKa = 3.63EE70 pKa = 4.84AYY72 pKa = 10.6GEE74 pKa = 4.0YY75 pKa = 10.53DD76 pKa = 3.33EE77 pKa = 5.69NKK79 pKa = 10.19IIEE82 pKa = 4.22LPKK85 pKa = 10.82NIFQIDD91 pKa = 3.61GKK93 pKa = 10.12IDD95 pKa = 3.5EE96 pKa = 5.4EE97 pKa = 4.45ILFEE101 pKa = 4.77GNTVPMMDD109 pKa = 3.57SEE111 pKa = 4.72GNRR114 pKa = 11.84LTGSIVSIGDD124 pKa = 4.13DD125 pKa = 2.97IVVMDD130 pKa = 5.12FNHH133 pKa = 6.82PLAGEE138 pKa = 4.14TMHH141 pKa = 7.3FKK143 pKa = 10.14GTVLNVRR150 pKa = 11.84DD151 pKa = 4.02ASAEE155 pKa = 3.91EE156 pKa = 3.8IAALFSAGSCSCGSDD171 pKa = 3.07SCGCGGEE178 pKa = 4.8SEE180 pKa = 4.86GCGCGSSGCNCC191 pKa = 4.2

Molecular weight: 20.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N4S6T6|A0A2N4S6T6_9BACT Carboxylic ester hydrolase OS=Dysgonamonadaceae bacterium OX=2065189 GN=C0T31_08980 PE=3 SV=1
RR1 pKa = 7.52QKK3 pKa = 9.76TSCLSRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.5AVSYY15 pKa = 10.94LSFSEE20 pKa = 4.14QAAIGSMRR28 pKa = 11.84RR29 pKa = 11.84CSLDD33 pKa = 2.96LFAYY37 pKa = 9.12FFASRR42 pKa = 11.84QKK44 pKa = 11.35SMWGLGQRR52 pKa = 11.84PKK54 pKa = 10.72NQWIMHH60 pKa = 5.58NVQLIDD66 pKa = 3.49LMIYY70 pKa = 10.35RR71 pKa = 11.84FTDD74 pKa = 3.14EE75 pKa = 4.26FRR77 pKa = 5.2

Molecular weight: 9.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2317

0

2317

801358

31

2492

345.9

39.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.617 ± 0.042

0.935 ± 0.02

5.697 ± 0.038

6.625 ± 0.049

4.96 ± 0.036

6.501 ± 0.048

1.849 ± 0.022

7.714 ± 0.041

6.913 ± 0.049

8.989 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.537 ± 0.022

5.447 ± 0.043

3.883 ± 0.028

3.563 ± 0.026

4.6 ± 0.033

6.413 ± 0.041

5.267 ± 0.034

6.176 ± 0.042

1.175 ± 0.022

4.141 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski