Moorella humiferrea

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Thermoanaerobacterales; Thermoanaerobacteraceae; Moorella group; Moorella

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2665 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T0AMQ6|A0A2T0AMQ6_9THEO Transport permease protein OS=Moorella humiferrea OX=676965 GN=yadH PE=3 SV=1
MM1 pKa = 7.08MSDD4 pKa = 3.12VRR6 pKa = 11.84KK7 pKa = 10.01KK8 pKa = 9.87VAYY11 pKa = 10.37LKK13 pKa = 11.03GLVDD17 pKa = 5.37GMDD20 pKa = 4.25LNPEE24 pKa = 4.04NKK26 pKa = 7.64EE27 pKa = 3.88TRR29 pKa = 11.84LLKK32 pKa = 10.61EE33 pKa = 3.62IVEE36 pKa = 4.41VLEE39 pKa = 4.01QMAEE43 pKa = 4.22AIHH46 pKa = 6.92DD47 pKa = 3.66LHH49 pKa = 7.08EE50 pKa = 5.26DD51 pKa = 3.7YY52 pKa = 11.44EE53 pKa = 4.3EE54 pKa = 5.05LEE56 pKa = 4.64EE57 pKa = 3.99YY58 pKa = 10.82LEE60 pKa = 5.6SIDD63 pKa = 4.07EE64 pKa = 4.25DD65 pKa = 4.73LYY67 pKa = 11.43DD68 pKa = 6.06LEE70 pKa = 5.75DD71 pKa = 3.28EE72 pKa = 4.94VYY74 pKa = 10.86AGDD77 pKa = 3.53EE78 pKa = 4.13DD79 pKa = 5.01EE80 pKa = 4.9YY81 pKa = 11.57EE82 pKa = 4.01EE83 pKa = 6.49DD84 pKa = 3.64EE85 pKa = 6.21DD86 pKa = 4.72EE87 pKa = 5.35DD88 pKa = 4.46EE89 pKa = 4.35EE90 pKa = 5.43DD91 pKa = 3.73EE92 pKa = 5.12EE93 pKa = 4.57EE94 pKa = 4.76EE95 pKa = 4.29EE96 pKa = 5.62DD97 pKa = 3.36EE98 pKa = 4.22GDD100 pKa = 4.08YY101 pKa = 11.23IEE103 pKa = 5.03VEE105 pKa = 4.25CPKK108 pKa = 10.26CHH110 pKa = 7.23EE111 pKa = 4.47IVCFDD116 pKa = 4.5ADD118 pKa = 3.77VLDD121 pKa = 5.19DD122 pKa = 4.1EE123 pKa = 5.78DD124 pKa = 4.42VVEE127 pKa = 4.73VTCPNCEE134 pKa = 3.64TVVFVNDD141 pKa = 3.42EE142 pKa = 4.21DD143 pKa = 4.21EE144 pKa = 4.24KK145 pKa = 11.48ASAIEE150 pKa = 4.44EE151 pKa = 4.26EE152 pKa = 4.63PPDD155 pKa = 4.71PKK157 pKa = 10.79DD158 pKa = 3.27TDD160 pKa = 4.41DD161 pKa = 3.35II162 pKa = 5.94

Molecular weight: 18.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T0AJT5|A0A2T0AJT5_9THEO Ferredoxin-1 OS=Moorella humiferrea OX=676965 GN=MOHU_27140 PE=4 SV=1
MM1 pKa = 7.24KK2 pKa = 10.37RR3 pKa = 11.84MKK5 pKa = 10.15RR6 pKa = 11.84KK7 pKa = 9.41HH8 pKa = 6.26LLQHH12 pKa = 5.54WRR14 pKa = 11.84LVVQILAFLLFVYY27 pKa = 10.41LLISGKK33 pKa = 10.12NKK35 pKa = 9.91LWMLIMMAFLLITPFWGRR53 pKa = 11.84LYY55 pKa = 10.82CGWLCPLNTLLRR67 pKa = 11.84PVNWLVSKK75 pKa = 10.53KK76 pKa = 10.26ARR78 pKa = 11.84RR79 pKa = 11.84LAKK82 pKa = 9.96DD83 pKa = 2.78IPLIGKK89 pKa = 7.99SPWLRR94 pKa = 11.84AVIFILFLLVFLMTLRR110 pKa = 11.84GIIKK114 pKa = 10.02INLIVLLAPFAILTTIFFTGAAWHH138 pKa = 7.09RR139 pKa = 11.84YY140 pKa = 7.6LCPFGVLFSLPAGVSRR156 pKa = 11.84WRR158 pKa = 11.84FHH160 pKa = 7.04INPDD164 pKa = 2.99RR165 pKa = 11.84CLACGRR171 pKa = 11.84CRR173 pKa = 11.84RR174 pKa = 11.84VCPAGAIAVNEE185 pKa = 4.16APEE188 pKa = 3.81KK189 pKa = 10.44SYY191 pKa = 11.01RR192 pKa = 11.84ILPRR196 pKa = 11.84YY197 pKa = 9.89CLFCFACQEE206 pKa = 4.04VCPRR210 pKa = 11.84SSIEE214 pKa = 3.68LTAARR219 pKa = 11.84PASSRR224 pKa = 11.84EE225 pKa = 3.6KK226 pKa = 8.64MTRR229 pKa = 11.84NLL231 pKa = 3.45

Molecular weight: 26.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2665

0

2665

780899

29

2008

293.0

32.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.138 ± 0.062

1.122 ± 0.023

4.461 ± 0.031

7.123 ± 0.059

3.569 ± 0.031

8.423 ± 0.045

1.742 ± 0.021

6.283 ± 0.04

4.7 ± 0.036

11.047 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.324 ± 0.022

3.102 ± 0.026

4.874 ± 0.03

3.26 ± 0.031

6.7 ± 0.048

4.22 ± 0.028

4.913 ± 0.033

7.769 ± 0.042

1.136 ± 0.021

3.095 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski