Gordonia phage Dogfish

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nyceiraevirus; unclassified Nyceiraevirus

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A481VUV5|A0A481VUV5_9CAUD Minor tail protein OS=Gordonia phage Dogfish OX=2530117 GN=20 PE=4 SV=1
MM1 pKa = 6.71STAIATAAVSAVLTLTACGGGSDD24 pKa = 4.19TPKK27 pKa = 10.39EE28 pKa = 3.92DD29 pKa = 3.53AFTGSGWVSTIITPLVPTSTGVAEE53 pKa = 4.94DD54 pKa = 4.36CDD56 pKa = 3.85HH57 pKa = 6.02VTDD60 pKa = 3.87EE61 pKa = 4.39GRR63 pKa = 11.84IKK65 pKa = 10.88GGVPVTISVGEE76 pKa = 4.14EE77 pKa = 4.09TVGSAILGVGKK88 pKa = 9.99FYY90 pKa = 10.98SDD92 pKa = 4.73SYY94 pKa = 11.31TSGCKK99 pKa = 7.71WQFSAEE105 pKa = 3.9ISRR108 pKa = 11.84TADD111 pKa = 3.07EE112 pKa = 4.44YY113 pKa = 10.88TVQIGDD119 pKa = 3.96SATSTASLDD128 pKa = 3.59EE129 pKa = 4.42LKK131 pKa = 10.92AGSFQFGTQVTAIYY145 pKa = 10.64

Molecular weight: 14.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A481VW16|A0A481VW16_9CAUD Minor tail protein OS=Gordonia phage Dogfish OX=2530117 GN=22 PE=4 SV=1
MM1 pKa = 7.45LRR3 pKa = 11.84FPEE6 pKa = 4.09RR7 pKa = 11.84WQIRR11 pKa = 11.84VPRR14 pKa = 11.84TTRR17 pKa = 11.84TDD19 pKa = 3.35DD20 pKa = 3.31TTGNQVPGPRR30 pKa = 11.84PTPVEE35 pKa = 4.08VNGSLEE41 pKa = 3.88QRR43 pKa = 11.84FPDD46 pKa = 3.32SDD48 pKa = 3.4QRR50 pKa = 11.84EE51 pKa = 4.26VGEE54 pKa = 4.73AIADD58 pKa = 3.63EE59 pKa = 4.31RR60 pKa = 11.84TLVLEE65 pKa = 4.56PAAHH69 pKa = 6.33AKK71 pKa = 9.28VAGGITKK78 pKa = 8.74QHH80 pKa = 5.45QAIGPDD86 pKa = 3.5GTVWSIVRR94 pKa = 11.84VPVPRR99 pKa = 11.84KK100 pKa = 9.22RR101 pKa = 11.84RR102 pKa = 11.84RR103 pKa = 11.84PSAPIRR109 pKa = 11.84YY110 pKa = 8.11LALVVRR116 pKa = 11.84RR117 pKa = 11.84STDD120 pKa = 2.64IKK122 pKa = 11.19EE123 pKa = 3.96NN124 pKa = 3.58

Molecular weight: 13.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

13492

47

1833

245.3

26.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.97 ± 0.659

0.734 ± 0.139

7.108 ± 0.198

5.396 ± 0.306

2.446 ± 0.176

9.094 ± 0.641

1.957 ± 0.242

4.291 ± 0.189

3.054 ± 0.229

8.057 ± 0.352

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.068 ± 0.179

2.742 ± 0.251

6.263 ± 0.419

3.773 ± 0.348

7.619 ± 0.466

5.455 ± 0.326

6.589 ± 0.246

7.056 ± 0.337

2.194 ± 0.271

2.135 ± 0.184
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski