Amino acid dipepetide frequency for Gordonia phage Dogfish

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.531AlaAla: 18.531 ± 2.24
0.593AlaCys: 0.593 ± 0.197
9.043AlaAsp: 9.043 ± 0.741
7.264AlaGlu: 7.264 ± 0.949
2.15AlaPhe: 2.15 ± 0.44
11.415AlaGly: 11.415 ± 1.444
1.927AlaHis: 1.927 ± 0.406
5.782AlaIle: 5.782 ± 0.661
3.261AlaLys: 3.261 ± 0.443
11.193AlaLeu: 11.193 ± 1.151
2.965AlaMet: 2.965 ± 0.52
3.632AlaAsn: 3.632 ± 0.625
4.522AlaPro: 4.522 ± 0.742
4.299AlaGln: 4.299 ± 0.572
8.672AlaArg: 8.672 ± 0.764
4.966AlaSer: 4.966 ± 0.777
7.264AlaThr: 7.264 ± 0.747
7.338AlaVal: 7.338 ± 0.675
1.927AlaTrp: 1.927 ± 0.407
2.891AlaTyr: 2.891 ± 0.531
0.0AlaXaa: 0.0 ± 0.0
Cys
0.741CysAla: 0.741 ± 0.266
0.148CysCys: 0.148 ± 0.11
0.815CysAsp: 0.815 ± 0.319
0.519CysGlu: 0.519 ± 0.211
0.0CysPhe: 0.0 ± 0.0
1.112CysGly: 1.112 ± 0.289
0.074CysHis: 0.074 ± 0.072
0.371CysIle: 0.371 ± 0.17
0.222CysLys: 0.222 ± 0.131
0.148CysLeu: 0.148 ± 0.113
0.074CysMet: 0.074 ± 0.057
0.222CysAsn: 0.222 ± 0.114
0.371CysPro: 0.371 ± 0.171
0.222CysGln: 0.222 ± 0.119
0.593CysArg: 0.593 ± 0.296
0.296CysSer: 0.296 ± 0.115
0.593CysThr: 0.593 ± 0.243
0.593CysVal: 0.593 ± 0.206
0.074CysTrp: 0.074 ± 0.077
0.148CysTyr: 0.148 ± 0.088
0.0CysXaa: 0.0 ± 0.0
Asp
8.895AspAla: 8.895 ± 0.735
0.741AspCys: 0.741 ± 0.229
4.892AspAsp: 4.892 ± 0.65
4.892AspGlu: 4.892 ± 0.565
1.334AspPhe: 1.334 ± 0.302
8.228AspGly: 8.228 ± 0.633
2.075AspHis: 2.075 ± 0.544
2.743AspIle: 2.743 ± 0.321
1.779AspLys: 1.779 ± 0.396
5.559AspLeu: 5.559 ± 0.641
1.112AspMet: 1.112 ± 0.238
1.112AspAsn: 1.112 ± 0.253
6.449AspPro: 6.449 ± 0.74
2.817AspGln: 2.817 ± 0.345
4.744AspArg: 4.744 ± 0.614
3.706AspSer: 3.706 ± 0.432
3.187AspThr: 3.187 ± 0.492
4.299AspVal: 4.299 ± 0.574
1.631AspTrp: 1.631 ± 0.429
0.889AspTyr: 0.889 ± 0.223
0.0AspXaa: 0.0 ± 0.0
Glu
6.004GluAla: 6.004 ± 0.8
0.667GluCys: 0.667 ± 0.214
3.632GluAsp: 3.632 ± 0.628
2.372GluGlu: 2.372 ± 0.432
1.927GluPhe: 1.927 ± 0.398
2.965GluGly: 2.965 ± 0.319
1.557GluHis: 1.557 ± 0.335
3.558GluIle: 3.558 ± 0.521
1.853GluLys: 1.853 ± 0.35
5.485GluLeu: 5.485 ± 0.529
1.482GluMet: 1.482 ± 0.267
1.853GluAsn: 1.853 ± 0.359
1.779GluPro: 1.779 ± 0.397
2.446GluGln: 2.446 ± 0.357
4.225GluArg: 4.225 ± 0.499
2.668GluSer: 2.668 ± 0.418
2.594GluThr: 2.594 ± 0.51
3.336GluVal: 3.336 ± 0.589
2.075GluTrp: 2.075 ± 0.341
1.482GluTyr: 1.482 ± 0.303
0.0GluXaa: 0.0 ± 0.0
Phe
2.817PheAla: 2.817 ± 0.478
0.074PheCys: 0.074 ± 0.097
1.927PheAsp: 1.927 ± 0.346
1.26PheGlu: 1.26 ± 0.362
0.593PhePhe: 0.593 ± 0.212
2.668PheGly: 2.668 ± 0.392
0.296PheHis: 0.296 ± 0.165
0.815PheIle: 0.815 ± 0.203
0.815PheLys: 0.815 ± 0.305
0.741PheLeu: 0.741 ± 0.199
0.296PheMet: 0.296 ± 0.112
1.26PheAsn: 1.26 ± 0.345
1.705PhePro: 1.705 ± 0.376
0.593PheGln: 0.593 ± 0.187
1.779PheArg: 1.779 ± 0.335
1.853PheSer: 1.853 ± 0.297
2.075PheThr: 2.075 ± 0.389
1.408PheVal: 1.408 ± 0.358
0.741PheTrp: 0.741 ± 0.287
0.741PheTyr: 0.741 ± 0.241
0.0PheXaa: 0.0 ± 0.0
Gly
9.858GlyAla: 9.858 ± 1.814
0.667GlyCys: 0.667 ± 0.243
6.226GlyAsp: 6.226 ± 0.728
4.373GlyGlu: 4.373 ± 0.644
2.446GlyPhe: 2.446 ± 0.406
9.71GlyGly: 9.71 ± 1.793
1.631GlyHis: 1.631 ± 0.264
2.817GlyIle: 2.817 ± 0.68
2.817GlyLys: 2.817 ± 0.334
7.931GlyLeu: 7.931 ± 0.612
1.853GlyMet: 1.853 ± 0.435
2.224GlyAsn: 2.224 ± 0.388
4.373GlyPro: 4.373 ± 0.622
4.077GlyGln: 4.077 ± 0.766
6.819GlyArg: 6.819 ± 0.886
6.523GlySer: 6.523 ± 0.739
6.375GlyThr: 6.375 ± 0.655
6.226GlyVal: 6.226 ± 0.788
2.298GlyTrp: 2.298 ± 0.346
1.705GlyTyr: 1.705 ± 0.355
0.0GlyXaa: 0.0 ± 0.0
His
1.853HisAla: 1.853 ± 0.316
0.222HisCys: 0.222 ± 0.138
1.334HisAsp: 1.334 ± 0.3
1.038HisGlu: 1.038 ± 0.224
0.445HisPhe: 0.445 ± 0.211
1.853HisGly: 1.853 ± 0.376
0.296HisHis: 0.296 ± 0.168
0.815HisIle: 0.815 ± 0.263
0.593HisLys: 0.593 ± 0.174
1.408HisLeu: 1.408 ± 0.304
0.371HisMet: 0.371 ± 0.176
0.222HisAsn: 0.222 ± 0.132
2.075HisPro: 2.075 ± 0.425
0.519HisGln: 0.519 ± 0.175
1.779HisArg: 1.779 ± 0.38
0.815HisSer: 0.815 ± 0.251
0.889HisThr: 0.889 ± 0.173
1.779HisVal: 1.779 ± 0.328
0.593HisTrp: 0.593 ± 0.202
0.667HisTyr: 0.667 ± 0.242
0.0HisXaa: 0.0 ± 0.0
Ile
5.633IleAla: 5.633 ± 0.692
0.445IleCys: 0.445 ± 0.19
3.336IleAsp: 3.336 ± 0.434
3.336IleGlu: 3.336 ± 0.458
0.964IlePhe: 0.964 ± 0.241
3.78IleGly: 3.78 ± 0.526
0.815IleHis: 0.815 ± 0.241
1.186IleIle: 1.186 ± 0.243
0.964IleLys: 0.964 ± 0.255
2.594IleLeu: 2.594 ± 0.407
0.519IleMet: 0.519 ± 0.198
0.964IleAsn: 0.964 ± 0.27
2.668IlePro: 2.668 ± 0.457
1.112IleGln: 1.112 ± 0.213
3.261IleArg: 3.261 ± 0.45
2.743IleSer: 2.743 ± 0.38
2.668IleThr: 2.668 ± 0.298
3.78IleVal: 3.78 ± 0.61
0.889IleTrp: 0.889 ± 0.263
1.26IleTyr: 1.26 ± 0.258
0.0IleXaa: 0.0 ± 0.0
Lys
3.929LysAla: 3.929 ± 0.665
0.148LysCys: 0.148 ± 0.108
1.26LysAsp: 1.26 ± 0.228
0.964LysGlu: 0.964 ± 0.285
0.889LysPhe: 0.889 ± 0.239
2.075LysGly: 2.075 ± 0.517
0.296LysHis: 0.296 ± 0.18
1.26LysIle: 1.26 ± 0.315
1.112LysLys: 1.112 ± 0.341
2.743LysLeu: 2.743 ± 0.474
0.667LysMet: 0.667 ± 0.279
0.445LysAsn: 0.445 ± 0.2
2.668LysPro: 2.668 ± 0.406
1.853LysGln: 1.853 ± 0.538
1.853LysArg: 1.853 ± 0.443
2.15LysSer: 2.15 ± 0.435
2.001LysThr: 2.001 ± 0.308
2.446LysVal: 2.446 ± 0.475
1.038LysTrp: 1.038 ± 0.368
0.741LysTyr: 0.741 ± 0.244
0.0LysXaa: 0.0 ± 0.0
Leu
10.451LeuAla: 10.451 ± 0.946
0.296LeuCys: 0.296 ± 0.156
5.856LeuAsp: 5.856 ± 0.676
4.447LeuGlu: 4.447 ± 0.511
2.15LeuPhe: 2.15 ± 0.303
5.782LeuGly: 5.782 ± 0.733
1.705LeuHis: 1.705 ± 0.356
3.113LeuIle: 3.113 ± 0.532
3.187LeuLys: 3.187 ± 0.451
6.152LeuLeu: 6.152 ± 0.666
1.927LeuMet: 1.927 ± 0.312
2.15LeuAsn: 2.15 ± 0.458
6.3LeuPro: 6.3 ± 0.742
2.52LeuGln: 2.52 ± 0.343
6.078LeuArg: 6.078 ± 0.524
4.151LeuSer: 4.151 ± 0.482
5.856LeuThr: 5.856 ± 0.664
5.856LeuVal: 5.856 ± 0.663
1.112LeuTrp: 1.112 ± 0.325
1.482LeuTyr: 1.482 ± 0.378
0.0LeuXaa: 0.0 ± 0.0
Met
2.372MetAla: 2.372 ± 0.402
0.148MetCys: 0.148 ± 0.093
1.186MetAsp: 1.186 ± 0.287
0.445MetGlu: 0.445 ± 0.181
0.222MetPhe: 0.222 ± 0.126
1.334MetGly: 1.334 ± 0.359
0.371MetHis: 0.371 ± 0.149
0.815MetIle: 0.815 ± 0.286
0.445MetLys: 0.445 ± 0.192
1.779MetLeu: 1.779 ± 0.36
0.296MetMet: 0.296 ± 0.151
0.964MetAsn: 0.964 ± 0.281
1.482MetPro: 1.482 ± 0.367
1.038MetGln: 1.038 ± 0.387
1.557MetArg: 1.557 ± 0.397
1.779MetSer: 1.779 ± 0.286
2.224MetThr: 2.224 ± 0.501
1.482MetVal: 1.482 ± 0.273
0.519MetTrp: 0.519 ± 0.158
0.222MetTyr: 0.222 ± 0.158
0.0MetXaa: 0.0 ± 0.0
Asn
3.113AsnAla: 3.113 ± 0.577
0.148AsnCys: 0.148 ± 0.092
2.15AsnAsp: 2.15 ± 0.339
1.26AsnGlu: 1.26 ± 0.272
0.593AsnPhe: 0.593 ± 0.164
3.484AsnGly: 3.484 ± 0.556
0.445AsnHis: 0.445 ± 0.191
0.667AsnIle: 0.667 ± 0.225
0.519AsnLys: 0.519 ± 0.188
1.927AsnLeu: 1.927 ± 0.41
0.741AsnMet: 0.741 ± 0.226
0.667AsnAsn: 0.667 ± 0.289
1.853AsnPro: 1.853 ± 0.325
1.26AsnGln: 1.26 ± 0.298
2.817AsnArg: 2.817 ± 0.4
1.334AsnSer: 1.334 ± 0.261
2.075AsnThr: 2.075 ± 0.574
1.26AsnVal: 1.26 ± 0.283
0.371AsnTrp: 0.371 ± 0.126
0.741AsnTyr: 0.741 ± 0.192
0.0AsnXaa: 0.0 ± 0.0
Pro
6.893ProAla: 6.893 ± 0.852
0.519ProCys: 0.519 ± 0.203
5.782ProAsp: 5.782 ± 0.61
3.929ProGlu: 3.929 ± 0.509
1.408ProPhe: 1.408 ± 0.296
5.782ProGly: 5.782 ± 0.535
1.26ProHis: 1.26 ± 0.352
2.52ProIle: 2.52 ± 0.36
2.075ProLys: 2.075 ± 0.43
4.151ProLeu: 4.151 ± 0.559
1.482ProMet: 1.482 ± 0.437
1.705ProAsn: 1.705 ± 0.283
5.485ProPro: 5.485 ± 1.027
2.52ProGln: 2.52 ± 0.338
3.41ProArg: 3.41 ± 0.545
3.187ProSer: 3.187 ± 0.703
4.225ProThr: 4.225 ± 0.731
3.78ProVal: 3.78 ± 0.506
1.26ProTrp: 1.26 ± 0.342
1.26ProTyr: 1.26 ± 0.364
0.0ProXaa: 0.0 ± 0.0
Gln
3.929GlnAla: 3.929 ± 0.654
0.074GlnCys: 0.074 ± 0.07
1.557GlnAsp: 1.557 ± 0.36
1.557GlnGlu: 1.557 ± 0.31
1.26GlnPhe: 1.26 ± 0.281
2.446GlnGly: 2.446 ± 0.473
0.815GlnHis: 0.815 ± 0.242
2.594GlnIle: 2.594 ± 0.411
1.334GlnLys: 1.334 ± 0.334
3.113GlnLeu: 3.113 ± 0.45
0.889GlnMet: 0.889 ± 0.249
1.334GlnAsn: 1.334 ± 0.25
2.372GlnPro: 2.372 ± 0.451
1.557GlnGln: 1.557 ± 0.387
3.484GlnArg: 3.484 ± 0.433
1.927GlnSer: 1.927 ± 0.423
2.668GlnThr: 2.668 ± 0.453
2.965GlnVal: 2.965 ± 0.464
0.815GlnTrp: 0.815 ± 0.232
1.038GlnTyr: 1.038 ± 0.26
0.0GlnXaa: 0.0 ± 0.0
Arg
7.042ArgAla: 7.042 ± 0.738
0.445ArgCys: 0.445 ± 0.18
4.522ArgAsp: 4.522 ± 0.557
4.225ArgGlu: 4.225 ± 0.567
2.446ArgPhe: 2.446 ± 0.452
6.004ArgGly: 6.004 ± 0.719
2.075ArgHis: 2.075 ± 0.439
4.003ArgIle: 4.003 ± 0.452
2.52ArgLys: 2.52 ± 0.348
6.523ArgLeu: 6.523 ± 0.741
2.001ArgMet: 2.001 ± 0.381
1.853ArgAsn: 1.853 ± 0.402
3.558ArgPro: 3.558 ± 0.58
2.52ArgGln: 2.52 ± 0.436
8.079ArgArg: 8.079 ± 0.863
4.077ArgSer: 4.077 ± 0.728
4.522ArgThr: 4.522 ± 0.549
6.152ArgVal: 6.152 ± 0.811
1.779ArgTrp: 1.779 ± 0.426
1.853ArgTyr: 1.853 ± 0.312
0.0ArgXaa: 0.0 ± 0.0
Ser
6.152SerAla: 6.152 ± 0.654
0.296SerCys: 0.296 ± 0.165
4.225SerAsp: 4.225 ± 0.564
2.668SerGlu: 2.668 ± 0.466
1.26SerPhe: 1.26 ± 0.293
5.633SerGly: 5.633 ± 0.689
0.519SerHis: 0.519 ± 0.169
2.594SerIle: 2.594 ± 0.485
2.075SerLys: 2.075 ± 0.316
4.003SerLeu: 4.003 ± 0.491
1.186SerMet: 1.186 ± 0.366
1.927SerAsn: 1.927 ± 0.358
2.743SerPro: 2.743 ± 0.452
2.001SerGln: 2.001 ± 0.349
3.41SerArg: 3.41 ± 0.526
3.039SerSer: 3.039 ± 0.537
5.04SerThr: 5.04 ± 0.612
4.299SerVal: 4.299 ± 0.448
0.741SerTrp: 0.741 ± 0.202
0.741SerTyr: 0.741 ± 0.185
0.0SerXaa: 0.0 ± 0.0
Thr
9.636ThrAla: 9.636 ± 0.871
0.296ThrCys: 0.296 ± 0.173
5.115ThrAsp: 5.115 ± 0.437
3.113ThrGlu: 3.113 ± 0.414
1.482ThrPhe: 1.482 ± 0.297
5.93ThrGly: 5.93 ± 0.551
0.964ThrHis: 0.964 ± 0.269
2.817ThrIle: 2.817 ± 0.451
2.001ThrLys: 2.001 ± 0.453
4.596ThrLeu: 4.596 ± 0.558
0.964ThrMet: 0.964 ± 0.25
1.927ThrAsn: 1.927 ± 0.381
5.93ThrPro: 5.93 ± 0.78
1.779ThrGln: 1.779 ± 0.328
4.225ThrArg: 4.225 ± 0.636
2.965ThrSer: 2.965 ± 0.433
4.744ThrThr: 4.744 ± 0.558
4.966ThrVal: 4.966 ± 0.562
1.482ThrTrp: 1.482 ± 0.338
0.964ThrTyr: 0.964 ± 0.284
0.0ThrXaa: 0.0 ± 0.0
Val
8.079ValAla: 8.079 ± 0.907
0.741ValCys: 0.741 ± 0.265
5.559ValAsp: 5.559 ± 0.641
4.077ValGlu: 4.077 ± 0.609
1.334ValPhe: 1.334 ± 0.298
7.042ValGly: 7.042 ± 0.608
1.408ValHis: 1.408 ± 0.317
2.372ValIle: 2.372 ± 0.443
2.001ValLys: 2.001 ± 0.347
6.078ValLeu: 6.078 ± 0.656
1.26ValMet: 1.26 ± 0.314
1.705ValAsn: 1.705 ± 0.314
4.596ValPro: 4.596 ± 0.679
2.594ValGln: 2.594 ± 0.394
5.856ValArg: 5.856 ± 0.72
3.632ValSer: 3.632 ± 0.411
4.151ValThr: 4.151 ± 0.791
5.04ValVal: 5.04 ± 0.567
1.631ValTrp: 1.631 ± 0.399
1.408ValTyr: 1.408 ± 0.398
0.0ValXaa: 0.0 ± 0.0
Trp
1.408TrpAla: 1.408 ± 0.316
0.519TrpCys: 0.519 ± 0.216
1.112TrpAsp: 1.112 ± 0.336
1.112TrpGlu: 1.112 ± 0.304
0.667TrpPhe: 0.667 ± 0.283
1.853TrpGly: 1.853 ± 0.321
0.667TrpHis: 0.667 ± 0.241
0.964TrpIle: 0.964 ± 0.233
0.371TrpLys: 0.371 ± 0.212
2.001TrpLeu: 2.001 ± 0.401
0.296TrpMet: 0.296 ± 0.144
0.741TrpAsn: 0.741 ± 0.247
1.038TrpPro: 1.038 ± 0.287
1.26TrpGln: 1.26 ± 0.21
1.853TrpArg: 1.853 ± 0.378
1.408TrpSer: 1.408 ± 0.313
1.408TrpThr: 1.408 ± 0.354
1.927TrpVal: 1.927 ± 0.372
0.667TrpTrp: 0.667 ± 0.209
0.667TrpTyr: 0.667 ± 0.217
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.372TyrAla: 2.372 ± 0.473
0.148TyrCys: 0.148 ± 0.116
1.557TyrAsp: 1.557 ± 0.319
1.112TyrGlu: 1.112 ± 0.271
0.889TyrPhe: 0.889 ± 0.286
1.853TyrGly: 1.853 ± 0.517
0.371TyrHis: 0.371 ± 0.147
1.112TyrIle: 1.112 ± 0.315
0.593TyrLys: 0.593 ± 0.28
2.446TyrLeu: 2.446 ± 0.346
0.222TyrMet: 0.222 ± 0.13
0.519TyrAsn: 0.519 ± 0.189
0.667TyrPro: 0.667 ± 0.242
0.741TyrGln: 0.741 ± 0.224
1.705TyrArg: 1.705 ± 0.288
1.334TyrSer: 1.334 ± 0.326
1.334TyrThr: 1.334 ± 0.309
1.631TyrVal: 1.631 ± 0.339
0.296TyrTrp: 0.296 ± 0.15
0.445TyrTyr: 0.445 ± 0.196
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (13492 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski