Pompano iridovirus
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 121 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3Q9EFX1|A0A3Q9EFX1_ISKNV Uncharacterized protein OS=Pompano iridovirus OX=2494350 GN=ORF17 PE=4 SV=1
MM1 pKa = 7.9 SSEE4 pKa = 3.94 LTLWRR9 pKa = 11.84 SVADD13 pKa = 3.62 VCGATYY19 pKa = 10.25 PLVRR23 pKa = 11.84 SYY25 pKa = 11.16 VAALEE30 pKa = 4.43 SGCDD34 pKa = 2.93 SGTTFRR40 pKa = 11.84 NYY42 pKa = 7.96 WSMRR46 pKa = 11.84 RR47 pKa = 11.84 AHH49 pKa = 6.78 IMAGNPFEE57 pKa = 5.94 DD58 pKa = 3.94 DD59 pKa = 4.82 DD60 pKa = 4.52 YY61 pKa = 11.49 FEE63 pKa = 4.54 TMSGVVISMATVAEE77 pKa = 4.62 HH78 pKa = 7.14 CDD80 pKa = 3.65 SLPDD84 pKa = 4.66 LLATYY89 pKa = 9.95 NAAAQTAPVFDD100 pKa = 4.61 CPMLQGFLDD109 pKa = 3.58 TVMQSEE115 pKa = 4.91 LLQDD119 pKa = 3.07 IHH121 pKa = 8.59 RR122 pKa = 11.84 DD123 pKa = 3.31 VAAGGNSANLLNIISHH139 pKa = 7.03 NIGKK143 pKa = 9.36 HH144 pKa = 5.79 DD145 pKa = 4.35 DD146 pKa = 4.28 FIKK149 pKa = 10.68 HH150 pKa = 5.84 FSGDD154 pKa = 3.14 IIGYY158 pKa = 9.46 LEE160 pKa = 4.14 TMPVDD165 pKa = 4.11 EE166 pKa = 5.35 LAQMMEE172 pKa = 4.67 VIKK175 pKa = 10.77 EE176 pKa = 3.96 MTGQGGDD183 pKa = 3.79 EE184 pKa = 4.86 LIQAIMAHH192 pKa = 6.14 APVV195 pKa = 3.65
Molecular weight: 21.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.006
IPC2_protein 4.291
IPC_protein 4.253
Toseland 4.075
ProMoST 4.393
Dawson 4.228
Bjellqvist 4.38
Wikipedia 4.139
Rodwell 4.088
Grimsley 3.973
Solomon 4.228
Lehninger 4.177
Nozaki 4.342
DTASelect 4.558
Thurlkill 4.101
EMBOSS 4.164
Sillero 4.38
Patrickios 1.99
IPC_peptide 4.228
IPC2_peptide 4.355
IPC2.peptide.svr19 4.261
Protein with the highest isoelectric point:
>tr|A0A3S9LM02|A0A3S9LM02_ISKNV Uncharacterized protein OS=Pompano iridovirus OX=2494350 GN=ORF4 PE=4 SV=1
MM1 pKa = 7.21 PQPIIFKK8 pKa = 9.06 CTASPSALRR17 pKa = 11.84 RR18 pKa = 11.84 YY19 pKa = 6.56 TASPRR24 pKa = 11.84 KK25 pKa = 9.33 RR26 pKa = 11.84 KK27 pKa = 10.14 SPAKK31 pKa = 9.65 RR32 pKa = 11.84 RR33 pKa = 11.84 SPAKK37 pKa = 9.51 RR38 pKa = 11.84 RR39 pKa = 11.84 SPVKK43 pKa = 9.77 RR44 pKa = 11.84 RR45 pKa = 11.84 SPVKK49 pKa = 9.81 RR50 pKa = 11.84 RR51 pKa = 11.84 SPVKK55 pKa = 9.81 RR56 pKa = 11.84 RR57 pKa = 11.84 SPVKK61 pKa = 9.65 RR62 pKa = 11.84 RR63 pKa = 11.84 SPARR67 pKa = 11.84 RR68 pKa = 11.84 RR69 pKa = 11.84 SPARR73 pKa = 11.84 RR74 pKa = 11.84 RR75 pKa = 3.46
Molecular weight: 8.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.483
IPC2_protein 11.169
IPC_protein 12.647
Toseland 12.808
ProMoST 13.305
Dawson 12.808
Bjellqvist 12.808
Wikipedia 13.29
Rodwell 12.471
Grimsley 12.852
Solomon 13.305
Lehninger 13.203
Nozaki 12.808
DTASelect 12.808
Thurlkill 12.808
EMBOSS 13.305
Sillero 12.808
Patrickios 12.179
IPC_peptide 13.305
IPC2_peptide 12.296
IPC2.peptide.svr19 9.119
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
121
0
121
36172
44
1309
298.9
33.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.612 ± 0.27
3.044 ± 0.231
6.088 ± 0.171
3.973 ± 0.187
3.022 ± 0.126
5.554 ± 0.181
3.677 ± 0.142
4.584 ± 0.141
3.417 ± 0.16
8.153 ± 0.216
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
4.136 ± 0.132
3.718 ± 0.122
5.504 ± 0.251
3.848 ± 0.14
6.312 ± 0.23
5.631 ± 0.171
7.083 ± 0.182
8.617 ± 0.263
1.111 ± 0.066
3.917 ± 0.118
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here