Brenneria sp. L3-3HA

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Pectobacteriaceae; Affinibrenneria

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4605 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J5FSH9|A0A5J5FSH9_9GAMM Zinc metalloprotease OS=Brenneria sp. L3-3HA OX=2590031 GN=rseP PE=3 SV=1
LL1 pKa = 6.98PAGAVAGDD9 pKa = 3.96TLSVTDD15 pKa = 5.26GATQQNVVLTAEE27 pKa = 4.46QISNGYY33 pKa = 9.76VDD35 pKa = 3.72TAFANPGEE43 pKa = 4.35GQTIQVDD50 pKa = 3.83ATLHH54 pKa = 5.34DD55 pKa = 3.58QYY57 pKa = 11.79GNVSDD62 pKa = 4.41TGSDD66 pKa = 3.29SARR69 pKa = 11.84IDD71 pKa = 3.79TLSGADD77 pKa = 3.21NSAPVVTITEE87 pKa = 4.14DD88 pKa = 3.3ANNDD92 pKa = 3.37GVISQSEE99 pKa = 4.27LQGQIDD105 pKa = 3.92VRR107 pKa = 11.84VGLPAGAVAGDD118 pKa = 3.96TLSVTDD124 pKa = 5.26GATQQNVVLTAEE136 pKa = 4.46QISNGYY142 pKa = 9.59VDD144 pKa = 4.04TAFASPGEE152 pKa = 4.15GQTIKK157 pKa = 10.71VDD159 pKa = 3.55ATLHH163 pKa = 5.39DD164 pKa = 3.57QYY166 pKa = 11.79GNVSDD171 pKa = 4.41TGSDD175 pKa = 3.4SARR178 pKa = 11.84VDD180 pKa = 3.76TLSGADD186 pKa = 3.21NSAPVVTITEE196 pKa = 4.14DD197 pKa = 3.3ANNDD201 pKa = 3.3GVISQTEE208 pKa = 4.04LQGQIDD214 pKa = 4.06VRR216 pKa = 11.84VGLPAGAVAGDD227 pKa = 3.96TLSVTDD233 pKa = 5.26GATQQNVVLTAEE245 pKa = 4.46QISNGYY251 pKa = 9.59VDD253 pKa = 3.96TAFASPAEE261 pKa = 4.16GQTIKK266 pKa = 10.74VDD268 pKa = 3.55ATLHH272 pKa = 5.39DD273 pKa = 3.57QYY275 pKa = 11.79GNVSDD280 pKa = 4.3TGSDD284 pKa = 3.34SALLDD289 pKa = 3.48TTPPDD294 pKa = 3.61TTITTVSGAEE304 pKa = 4.03DD305 pKa = 3.9TPLTINWSDD314 pKa = 3.8LGVTADD320 pKa = 3.46TATVVINSLPDD331 pKa = 3.25AGIGVLQYY339 pKa = 10.8NGQAVVQGQTFTAADD354 pKa = 3.81LVDD357 pKa = 4.51GKK359 pKa = 10.93LQFIPQEE366 pKa = 4.04NVAGSEE372 pKa = 4.01PTPAITFQPVDD383 pKa = 3.2AVGNSGGVSTLNVDD397 pKa = 3.9IAPVADD403 pKa = 3.73TPVVNVGVTNGDD415 pKa = 3.35TTEE418 pKa = 4.23SVSEE422 pKa = 4.14IIKK425 pKa = 10.55VNGGSEE431 pKa = 4.43SVGGFDD437 pKa = 3.87VQDD440 pKa = 3.54GKK442 pKa = 10.69IVKK445 pKa = 9.8IGDD448 pKa = 3.81GVRR451 pKa = 11.84VWLTEE456 pKa = 3.83GDD458 pKa = 4.28SVPEE462 pKa = 3.93SSNPAEE468 pKa = 3.96QIKK471 pKa = 10.79YY472 pKa = 10.27YY473 pKa = 10.76SQTNNSGSTDD483 pKa = 3.48YY484 pKa = 11.92ADD486 pKa = 3.11IFVVHH491 pKa = 6.25QDD493 pKa = 2.16SGYY496 pKa = 10.95YY497 pKa = 9.4YY498 pKa = 10.3RR499 pKa = 11.84QSDD502 pKa = 3.02WSEE505 pKa = 3.82DD506 pKa = 3.54QKK508 pKa = 10.89QLRR511 pKa = 11.84TLDD514 pKa = 3.8SVSGNSHH521 pKa = 5.11SQSTNSGKK529 pKa = 10.45DD530 pKa = 3.32YY531 pKa = 11.11IFVQQEE537 pKa = 3.71DD538 pKa = 5.27GYY540 pKa = 10.45SYY542 pKa = 11.37SVSNSTNNRR551 pKa = 11.84NNNFNTLDD559 pKa = 3.56GVTVKK564 pKa = 9.88STDD567 pKa = 3.35SNGNSVSLINQVSNQLEE584 pKa = 4.15GVIFGDD590 pKa = 3.84GTSYY594 pKa = 9.9TADD597 pKa = 3.17SKK599 pKa = 10.73ATSIEE604 pKa = 4.37TVAGAPGYY612 pKa = 8.74QEE614 pKa = 5.09HH615 pKa = 6.98VINVAASLTDD625 pKa = 3.44TDD627 pKa = 4.21GSEE630 pKa = 4.15SLSGITLSGLPEE642 pKa = 3.95GTEE645 pKa = 3.89IVSNGQVVQTLGAGDD660 pKa = 4.03TYY662 pKa = 11.49YY663 pKa = 10.29IANSSDD669 pKa = 3.66SQSLSAQVTVRR680 pKa = 11.84VPVAAGKK687 pKa = 8.23FTVTAQADD695 pKa = 4.14SLEE698 pKa = 4.44HH699 pKa = 6.82ANGSHH704 pKa = 5.94ATGIDD709 pKa = 3.53TQTVEE714 pKa = 4.11QFSVIVGTSGDD725 pKa = 4.06DD726 pKa = 4.01NIHH729 pKa = 5.73GTPSNDD735 pKa = 2.4IVVSDD740 pKa = 3.64VTGLQMVEE748 pKa = 3.79GQNYY752 pKa = 9.72NIAFMVDD759 pKa = 3.15TSGSLSNADD768 pKa = 3.1IASTQASLTKK778 pKa = 10.41VFNSLISSANGANSGTVNVFLADD801 pKa = 3.88FDD803 pKa = 4.35TQVGKK808 pKa = 8.2TVSVNLADD816 pKa = 4.54SDD818 pKa = 4.32ALSKK822 pKa = 10.22LTTVLNSMIGGQNGGGTNYY841 pKa = 10.56EE842 pKa = 4.31DD843 pKa = 4.21VFKK846 pKa = 9.15TTANWFHH853 pKa = 7.25SDD855 pKa = 3.31TVTSNPGTNLTYY867 pKa = 10.44FITDD871 pKa = 4.09GQPTFYY877 pKa = 9.46QTNEE881 pKa = 4.06SNSVSVSDD889 pKa = 3.88WGQSLNIDD897 pKa = 3.72TLNYY901 pKa = 10.33KK902 pKa = 10.08PGQSYY907 pKa = 11.13SMNVRR912 pKa = 11.84GEE914 pKa = 3.95WKK916 pKa = 10.31EE917 pKa = 4.15VIDD920 pKa = 4.33SAGNVYY926 pKa = 10.28RR927 pKa = 11.84YY928 pKa = 9.29SWYY931 pKa = 10.57GKK933 pKa = 7.43EE934 pKa = 4.24TIGKK938 pKa = 9.54VNAQGDD944 pKa = 4.02GTYY947 pKa = 10.04EE948 pKa = 3.64ISKK951 pKa = 10.68LGGIGNNSSYY961 pKa = 10.75WNGRR965 pKa = 11.84TWVDD969 pKa = 3.52GAANAAASHH978 pKa = 6.28SNAGQAFTLLAALSLVEE995 pKa = 4.91SIGIGQDD1002 pKa = 3.37LNAADD1007 pKa = 4.47LKK1009 pKa = 11.29NYY1011 pKa = 10.49DD1012 pKa = 3.21SDD1014 pKa = 4.62GVVQSHH1020 pKa = 7.21IDD1022 pKa = 3.6PSDD1025 pKa = 3.33LTEE1028 pKa = 5.73AILGSNTLLPAGSDD1042 pKa = 3.27HH1043 pKa = 5.56VTGGVGDD1050 pKa = 5.47DD1051 pKa = 3.46ILFGDD1056 pKa = 4.37SVTFDD1061 pKa = 4.39GISSNGYY1068 pKa = 6.84TALHH1072 pKa = 6.49TYY1074 pKa = 10.05VAGQMGLQEE1083 pKa = 4.57SDD1085 pKa = 3.15VTAQQVHH1092 pKa = 5.32QYY1094 pKa = 9.97IKK1096 pKa = 10.53EE1097 pKa = 3.85NSADD1101 pKa = 3.78FSHH1104 pKa = 7.26SSSNDD1109 pKa = 3.32GNDD1112 pKa = 3.17TLYY1115 pKa = 11.26GGDD1118 pKa = 4.51GDD1120 pKa = 5.85DD1121 pKa = 3.67ILYY1124 pKa = 10.12GQGGDD1129 pKa = 3.41DD1130 pKa = 3.67TLYY1133 pKa = 11.16GGAGNDD1139 pKa = 3.08ILYY1142 pKa = 10.54GGSGNDD1148 pKa = 3.37TLYY1151 pKa = 11.44GDD1153 pKa = 5.14DD1154 pKa = 5.71GNDD1157 pKa = 3.32LLYY1160 pKa = 11.13GEE1162 pKa = 5.19EE1163 pKa = 4.83GDD1165 pKa = 5.69DD1166 pKa = 3.88ILHH1169 pKa = 6.64GGAGNDD1175 pKa = 3.5TLYY1178 pKa = 11.1GGSGNDD1184 pKa = 3.28TLYY1187 pKa = 11.32GDD1189 pKa = 4.42EE1190 pKa = 5.42GNDD1193 pKa = 3.32TLYY1196 pKa = 11.18GGEE1199 pKa = 4.85GDD1201 pKa = 3.89DD1202 pKa = 4.67KK1203 pKa = 11.58LYY1205 pKa = 11.36GGAGNDD1211 pKa = 3.47TLYY1214 pKa = 11.21GGTGNDD1220 pKa = 3.51ILNGGDD1226 pKa = 4.04GDD1228 pKa = 5.29DD1229 pKa = 4.29ILIGGPGDD1237 pKa = 3.48NTLTGGAGADD1247 pKa = 3.53TFKK1250 pKa = 10.48WQAGDD1255 pKa = 3.41VGHH1258 pKa = 7.43DD1259 pKa = 3.51VIKK1262 pKa = 10.77DD1263 pKa = 3.67FNASEE1268 pKa = 4.21GDD1270 pKa = 3.97KK1271 pKa = 10.52IDD1273 pKa = 4.77LSDD1276 pKa = 3.8LVGEE1280 pKa = 4.89LEE1282 pKa = 4.27HH1283 pKa = 6.44GTDD1286 pKa = 2.86ISHH1289 pKa = 7.58YY1290 pKa = 10.16IRR1292 pKa = 11.84IVDD1295 pKa = 3.89DD1296 pKa = 3.25QGSPVIEE1303 pKa = 4.38VNTAGQFGTPNATADD1318 pKa = 3.4VTITLEE1324 pKa = 4.46HH1325 pKa = 6.58YY1326 pKa = 10.56SGSLPSLDD1334 pKa = 3.8SLISKK1339 pKa = 9.64PEE1341 pKa = 3.54QSTII1345 pKa = 3.41

Molecular weight: 139.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J5FY41|A0A5J5FY41_9GAMM Autoinducer 2 ABC transporter substrate-binding protein OS=Brenneria sp. L3-3HA OX=2590031 GN=FJU30_16070 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.36GRR39 pKa = 11.84TRR41 pKa = 11.84LTVSKK46 pKa = 10.95

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4605

0

4605

1471549

14

2871

319.6

35.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.431 ± 0.042

1.113 ± 0.014

5.276 ± 0.03

5.2 ± 0.035

3.758 ± 0.026

7.512 ± 0.034

2.208 ± 0.017

5.735 ± 0.031

3.497 ± 0.033

11.364 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.503 ± 0.016

3.403 ± 0.025

4.646 ± 0.026

4.779 ± 0.031

6.554 ± 0.035

6.023 ± 0.028

4.957 ± 0.023

6.726 ± 0.025

1.49 ± 0.015

2.827 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski