Amino acid dipepetide frequency for Brenneria sp. L3-3HA

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.92AlaAla: 11.92 ± 0.129	1.244AlaCys: 1.244 ± 0.036	5.534AlaAsp: 5.534 ± 0.067	5.484AlaGlu: 5.484 ± 0.08	3.682AlaPhe: 3.682 ± 0.053	9.022AlaGly: 9.022 ± 0.087	1.91AlaHis: 1.91 ± 0.037	5.973AlaIle: 5.973 ± 0.075	2.899AlaLys: 2.899 ± 0.056	13.618AlaLeu: 13.618 ± 0.124	2.769AlaMet: 2.769 ± 0.049	2.685AlaAsn: 2.685 ± 0.046	4.391AlaPro: 4.391 ± 0.062	5.164AlaGln: 5.164 ± 0.068	7.127AlaArg: 7.127 ± 0.087	5.943AlaSer: 5.943 ± 0.061	4.497AlaThr: 4.497 ± 0.057	6.75AlaVal: 6.75 ± 0.068	1.687AlaTrp: 1.687 ± 0.033	2.003AlaTyr: 2.003 ± 0.037	0.001AlaXaa: 0.001 ± 0.001
Cys	1.157CysAla: 1.157 ± 0.03	0.188CysCys: 0.188 ± 0.013	0.572CysAsp: 0.572 ± 0.021	0.562CysGlu: 0.562 ± 0.021	0.436CysPhe: 0.436 ± 0.021	1.112CysGly: 1.112 ± 0.03	0.301CysHis: 0.301 ± 0.016	0.55CysIle: 0.55 ± 0.024	0.27CysLys: 0.27 ± 0.015	1.203CysLeu: 1.203 ± 0.029	0.236CysMet: 0.236 ± 0.014	0.311CysAsn: 0.311 ± 0.015	0.504CysPro: 0.504 ± 0.019	0.447CysGln: 0.447 ± 0.018	0.871CysArg: 0.871 ± 0.029	0.712CysSer: 0.712 ± 0.021	0.432CysThr: 0.432 ± 0.018	0.733CysVal: 0.733 ± 0.022	0.179CysTrp: 0.179 ± 0.009	0.354CysTyr: 0.354 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	5.41AspAla: 5.41 ± 0.06	0.55AspCys: 0.55 ± 0.018	3.18AspAsp: 3.18 ± 0.065	3.603AspGlu: 3.603 ± 0.055	2.172AspPhe: 2.172 ± 0.039	4.206AspGly: 4.206 ± 0.055	0.941AspHis: 0.941 ± 0.025	3.582AspIle: 3.582 ± 0.05	2.154AspLys: 2.154 ± 0.044	4.815AspLeu: 4.815 ± 0.056	1.286AspMet: 1.286 ± 0.031	2.04AspAsn: 2.04 ± 0.036	2.252AspPro: 2.252 ± 0.04	1.645AspGln: 1.645 ± 0.034	3.16AspArg: 3.16 ± 0.047	2.949AspSer: 2.949 ± 0.044	2.364AspThr: 2.364 ± 0.045	3.602AspVal: 3.602 ± 0.049	0.953AspTrp: 0.953 ± 0.021	1.901AspTyr: 1.901 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	4.959GluAla: 4.959 ± 0.079	0.429GluCys: 0.429 ± 0.019	2.095GluAsp: 2.095 ± 0.039	2.775GluGlu: 2.775 ± 0.051	1.652GluPhe: 1.652 ± 0.035	3.03GluGly: 3.03 ± 0.046	1.268GluHis: 1.268 ± 0.029	3.159GluIle: 3.159 ± 0.057	2.688GluLys: 2.688 ± 0.047	5.721GluLeu: 5.721 ± 0.078	1.541GluMet: 1.541 ± 0.035	2.128GluAsn: 2.128 ± 0.042	2.038GluPro: 2.038 ± 0.044	3.618GluGln: 3.618 ± 0.054	4.143GluArg: 4.143 ± 0.06	2.695GluSer: 2.695 ± 0.042	2.698GluThr: 2.698 ± 0.042	3.309GluVal: 3.309 ± 0.05	0.673GluTrp: 0.673 ± 0.021	1.376GluTyr: 1.376 ± 0.032	0.0GluXaa: 0.0 ± 0.0
Phe	3.725PheAla: 3.725 ± 0.056	0.55PheCys: 0.55 ± 0.019	2.304PheAsp: 2.304 ± 0.042	1.574PheGlu: 1.574 ± 0.033	1.68PhePhe: 1.68 ± 0.04	3.078PheGly: 3.078 ± 0.046	0.841PheHis: 0.841 ± 0.025	2.626PheIle: 2.626 ± 0.042	1.058PheLys: 1.058 ± 0.026	3.337PheLeu: 3.337 ± 0.051	0.926PheMet: 0.926 ± 0.026	1.639PheAsn: 1.639 ± 0.036	1.569PhePro: 1.569 ± 0.032	1.11PheGln: 1.11 ± 0.029	1.969PheArg: 1.969 ± 0.035	3.234PheSer: 3.234 ± 0.046	2.124PheThr: 2.124 ± 0.041	2.394PheVal: 2.394 ± 0.044	0.574PheTrp: 0.574 ± 0.022	1.269PheTyr: 1.269 ± 0.033	0.0PheXaa: 0.0 ± 0.0
Gly	7.043GlyAla: 7.043 ± 0.083	1.049GlyCys: 1.049 ± 0.026	3.846GlyAsp: 3.846 ± 0.059	4.481GlyGlu: 4.481 ± 0.063	3.156GlyPhe: 3.156 ± 0.054	5.652GlyGly: 5.652 ± 0.073	1.599GlyHis: 1.599 ± 0.04	4.804GlyIle: 4.804 ± 0.067	3.415GlyLys: 3.415 ± 0.049	7.967GlyLeu: 7.967 ± 0.078	2.216GlyMet: 2.216 ± 0.039	2.526GlyAsn: 2.526 ± 0.044	2.143GlyPro: 2.143 ± 0.033	3.301GlyGln: 3.301 ± 0.052	4.63GlyArg: 4.63 ± 0.056	4.203GlySer: 4.203 ± 0.051	3.314GlyThr: 3.314 ± 0.052	5.748GlyVal: 5.748 ± 0.067	1.335GlyTrp: 1.335 ± 0.035	2.688GlyTyr: 2.688 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	1.992HisAla: 1.992 ± 0.032	0.334HisCys: 0.334 ± 0.016	1.178HisAsp: 1.178 ± 0.031	1.028HisGlu: 1.028 ± 0.028	1.011HisPhe: 1.011 ± 0.029	1.703HisGly: 1.703 ± 0.038	0.697HisHis: 0.697 ± 0.023	1.321HisIle: 1.321 ± 0.033	0.633HisLys: 0.633 ± 0.022	2.285HisLeu: 2.285 ± 0.044	0.454HisMet: 0.454 ± 0.02	0.797HisAsn: 0.797 ± 0.022	1.346HisPro: 1.346 ± 0.033	1.244HisGln: 1.244 ± 0.028	1.371HisArg: 1.371 ± 0.032	1.267HisSer: 1.267 ± 0.034	0.987HisThr: 0.987 ± 0.026	1.157HisVal: 1.157 ± 0.029	0.4HisTrp: 0.4 ± 0.018	0.873HisTyr: 0.873 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	6.686IleAla: 6.686 ± 0.075	0.692IleCys: 0.692 ± 0.022	3.667IleAsp: 3.667 ± 0.051	3.261IleGlu: 3.261 ± 0.054	1.99IlePhe: 1.99 ± 0.035	4.855IleGly: 4.855 ± 0.063	1.102IleHis: 1.102 ± 0.028	3.369IleIle: 3.369 ± 0.063	2.069IleLys: 2.069 ± 0.042	5.074IleLeu: 5.074 ± 0.062	1.267IleMet: 1.267 ± 0.028	2.471IleAsn: 2.471 ± 0.053	2.641IlePro: 2.641 ± 0.04	1.842IleGln: 1.842 ± 0.034	3.239IleArg: 3.239 ± 0.042	3.833IleSer: 3.833 ± 0.061	3.133IleThr: 3.133 ± 0.053	3.865IleVal: 3.865 ± 0.061	0.684IleTrp: 0.684 ± 0.024	1.611IleTyr: 1.611 ± 0.036	0.0IleXaa: 0.0 ± 0.0
Lys	3.331LysAla: 3.331 ± 0.061	0.221LysCys: 0.221 ± 0.013	1.64LysAsp: 1.64 ± 0.04	1.825LysGlu: 1.825 ± 0.045	0.936LysPhe: 0.936 ± 0.025	2.354LysGly: 2.354 ± 0.043	0.656LysHis: 0.656 ± 0.022	2.179LysIle: 2.179 ± 0.048	1.952LysLys: 1.952 ± 0.044	3.494LysLeu: 3.494 ± 0.056	1.005LysMet: 1.005 ± 0.025	1.584LysAsn: 1.584 ± 0.037	1.721LysPro: 1.721 ± 0.041	1.722LysGln: 1.722 ± 0.04	2.251LysArg: 2.251 ± 0.043	2.087LysSer: 2.087 ± 0.037	2.248LysThr: 2.248 ± 0.047	2.401LysVal: 2.401 ± 0.046	0.355LysTrp: 0.355 ± 0.014	1.009LysTyr: 1.009 ± 0.031	0.0LysXaa: 0.0 ± 0.0
Leu	13.05LeuAla: 13.05 ± 0.121	1.403LeuCys: 1.403 ± 0.031	5.811LeuAsp: 5.811 ± 0.067	5.144LeuGlu: 5.144 ± 0.067	4.347LeuPhe: 4.347 ± 0.069	7.404LeuGly: 7.404 ± 0.086	2.382LeuHis: 2.382 ± 0.04	6.058LeuIle: 6.058 ± 0.066	3.862LeuLys: 3.862 ± 0.057	13.327LeuLeu: 13.327 ± 0.167	2.722LeuMet: 2.722 ± 0.044	4.07LeuAsn: 4.07 ± 0.056	6.107LeuPro: 6.107 ± 0.071	4.641LeuGln: 4.641 ± 0.068	7.194LeuArg: 7.194 ± 0.081	8.182LeuSer: 8.182 ± 0.075	6.643LeuThr: 6.643 ± 0.08	7.02LeuVal: 7.02 ± 0.08	1.464LeuTrp: 1.464 ± 0.035	2.808LeuTyr: 2.808 ± 0.042	0.0LeuXaa: 0.0 ± 0.0
Met	2.772MetAla: 2.772 ± 0.041	0.177MetCys: 0.177 ± 0.01	1.117MetAsp: 1.117 ± 0.027	1.11MetGlu: 1.11 ± 0.033	0.762MetPhe: 0.762 ± 0.024	1.645MetGly: 1.645 ± 0.036	0.453MetHis: 0.453 ± 0.018	1.32MetIle: 1.32 ± 0.033	1.201MetLys: 1.201 ± 0.029	3.037MetLeu: 3.037 ± 0.05	0.761MetMet: 0.761 ± 0.026	1.003MetAsn: 1.003 ± 0.028	1.271MetPro: 1.271 ± 0.028	1.072MetGln: 1.072 ± 0.025	1.523MetArg: 1.523 ± 0.033	1.725MetSer: 1.725 ± 0.04	1.649MetThr: 1.649 ± 0.035	1.74MetVal: 1.74 ± 0.041	0.225MetTrp: 0.225 ± 0.012	0.465MetTyr: 0.465 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	3.306AsnAla: 3.306 ± 0.047	0.301AsnCys: 0.301 ± 0.015	1.911AsnAsp: 1.911 ± 0.037	1.681AsnGlu: 1.681 ± 0.035	1.15AsnPhe: 1.15 ± 0.027	2.799AsnGly: 2.799 ± 0.05	0.773AsnHis: 0.773 ± 0.022	2.219AsnIle: 2.219 ± 0.045	1.393AsnLys: 1.393 ± 0.036	3.292AsnLeu: 3.292 ± 0.049	0.808AsnMet: 0.808 ± 0.023	1.396AsnAsn: 1.396 ± 0.036	1.979AsnPro: 1.979 ± 0.041	1.589AsnGln: 1.589 ± 0.037	1.993AsnArg: 1.993 ± 0.032	1.909AsnSer: 1.909 ± 0.042	1.683AsnThr: 1.683 ± 0.036	2.267AsnVal: 2.267 ± 0.043	0.526AsnTrp: 0.526 ± 0.022	1.054AsnTyr: 1.054 ± 0.031	0.0AsnXaa: 0.0 ± 0.0
Pro	5.663ProAla: 5.663 ± 0.077	0.427ProCys: 0.427 ± 0.018	3.026ProAsp: 3.026 ± 0.052	2.994ProGlu: 2.994 ± 0.047	1.865ProPhe: 1.865 ± 0.036	3.723ProGly: 3.723 ± 0.049	1.076ProHis: 1.076 ± 0.026	1.918ProIle: 1.918 ± 0.04	1.18ProLys: 1.18 ± 0.03	5.657ProLeu: 5.657 ± 0.078	0.974ProMet: 0.974 ± 0.023	1.144ProAsn: 1.144 ± 0.025	2.002ProPro: 2.002 ± 0.043	2.464ProGln: 2.464 ± 0.042	2.331ProArg: 2.331 ± 0.042	2.281ProSer: 2.281 ± 0.041	1.988ProThr: 1.988 ± 0.045	3.712ProVal: 3.712 ± 0.055	0.792ProTrp: 0.792 ± 0.027	1.239ProTyr: 1.239 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	5.482GlnAla: 5.482 ± 0.067	0.419GlnCys: 0.419 ± 0.015	2.137GlnAsp: 2.137 ± 0.04	2.17GlnGlu: 2.17 ± 0.044	1.403GlnPhe: 1.403 ± 0.031	3.342GlnGly: 3.342 ± 0.044	1.262GlnHis: 1.262 ± 0.03	2.456GlnIle: 2.456 ± 0.039	1.517GlnLys: 1.517 ± 0.031	5.145GlnLeu: 5.145 ± 0.07	1.153GlnMet: 1.153 ± 0.028	1.367GlnAsn: 1.367 ± 0.032	2.599GlnPro: 2.599 ± 0.056	3.764GlnGln: 3.764 ± 0.069	3.945GlnArg: 3.945 ± 0.073	2.495GlnSer: 2.495 ± 0.045	2.313GlnThr: 2.313 ± 0.038	2.989GlnVal: 2.989 ± 0.045	0.659GlnTrp: 0.659 ± 0.022	1.174GlnTyr: 1.174 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	5.844ArgAla: 5.844 ± 0.07	0.678ArgCys: 0.678 ± 0.026	3.341ArgAsp: 3.341 ± 0.053	3.765ArgGlu: 3.765 ± 0.056	2.747ArgPhe: 2.747 ± 0.041	3.791ArgGly: 3.791 ± 0.05	1.962ArgHis: 1.962 ± 0.04	3.784ArgIle: 3.784 ± 0.048	2.142ArgLys: 2.142 ± 0.044	8.125ArgLeu: 8.125 ± 0.096	1.634ArgMet: 1.634 ± 0.034	1.967ArgAsn: 1.967 ± 0.038	2.845ArgPro: 2.845 ± 0.053	4.389ArgGln: 4.389 ± 0.076	5.138ArgArg: 5.138 ± 0.078	2.971ArgSer: 2.971 ± 0.048	2.597ArgThr: 2.597 ± 0.041	4.095ArgVal: 4.095 ± 0.063	1.145ArgTrp: 1.145 ± 0.036	2.584ArgTyr: 2.584 ± 0.046	0.0ArgXaa: 0.0 ± 0.0
Ser	6.531SerAla: 6.531 ± 0.077	0.621SerCys: 0.621 ± 0.021	3.132SerAsp: 3.132 ± 0.051	3.078SerGlu: 3.078 ± 0.052	2.325SerPhe: 2.325 ± 0.04	5.663SerGly: 5.663 ± 0.061	1.373SerHis: 1.373 ± 0.032	3.032SerIle: 3.032 ± 0.048	1.721SerLys: 1.721 ± 0.037	7.109SerLeu: 7.109 ± 0.091	1.399SerMet: 1.399 ± 0.029	1.683SerAsn: 1.683 ± 0.042	2.747SerPro: 2.747 ± 0.044	2.631SerGln: 2.631 ± 0.045	3.761SerArg: 3.761 ± 0.053	3.676SerSer: 3.676 ± 0.067	2.768SerThr: 2.768 ± 0.047	4.278SerVal: 4.278 ± 0.063	0.971SerTrp: 0.971 ± 0.027	1.733SerTyr: 1.733 ± 0.033	0.0SerXaa: 0.0 ± 0.0
Thr	4.72ThrAla: 4.72 ± 0.063	0.456ThrCys: 0.456 ± 0.019	2.476ThrAsp: 2.476 ± 0.038	2.347ThrGlu: 2.347 ± 0.045	1.84ThrPhe: 1.84 ± 0.037	4.075ThrGly: 4.075 ± 0.054	1.112ThrHis: 1.112 ± 0.027	2.635ThrIle: 2.635 ± 0.044	1.102ThrLys: 1.102 ± 0.031	7.525ThrLeu: 7.525 ± 0.087	0.98ThrMet: 0.98 ± 0.027	1.288ThrAsn: 1.288 ± 0.029	3.236ThrPro: 3.236 ± 0.043	2.008ThrGln: 2.008 ± 0.038	3.193ThrArg: 3.193 ± 0.048	2.798ThrSer: 2.798 ± 0.049	2.459ThrThr: 2.459 ± 0.04	3.592ThrVal: 3.592 ± 0.05	0.648ThrTrp: 0.648 ± 0.024	1.079ThrTyr: 1.079 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	7.15ValAla: 7.15 ± 0.08	0.824ValCys: 0.824 ± 0.026	3.584ValAsp: 3.584 ± 0.049	3.495ValGlu: 3.495 ± 0.062	2.567ValPhe: 2.567 ± 0.049	4.586ValGly: 4.586 ± 0.063	1.172ValHis: 1.172 ± 0.032	4.275ValIle: 4.275 ± 0.068	2.489ValLys: 2.489 ± 0.053	7.152ValLeu: 7.152 ± 0.081	1.98ValMet: 1.98 ± 0.038	2.595ValAsn: 2.595 ± 0.046	2.817ValPro: 2.817 ± 0.042	2.34ValGln: 2.34 ± 0.042	4.039ValArg: 4.039 ± 0.047	4.625ValSer: 4.625 ± 0.058	3.847ValThr: 3.847 ± 0.056	5.118ValVal: 5.118 ± 0.076	0.914ValTrp: 0.914 ± 0.029	1.687ValTyr: 1.687 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.033TrpAla: 1.033 ± 0.029	0.184TrpCys: 0.184 ± 0.012	0.595TrpAsp: 0.595 ± 0.02	0.491TrpGlu: 0.491 ± 0.019	0.65TrpPhe: 0.65 ± 0.023	0.881TrpGly: 0.881 ± 0.025	0.448TrpHis: 0.448 ± 0.018	0.679TrpIle: 0.679 ± 0.022	0.413TrpLys: 0.413 ± 0.015	2.506TrpLeu: 2.506 ± 0.054	0.367TrpMet: 0.367 ± 0.018	0.433TrpAsn: 0.433 ± 0.017	0.741TrpPro: 0.741 ± 0.022	1.12TrpGln: 1.12 ± 0.029	1.445TrpArg: 1.445 ± 0.037	0.868TrpSer: 0.868 ± 0.024	0.536TrpThr: 0.536 ± 0.02	0.864TrpVal: 0.864 ± 0.022	0.256TrpTrp: 0.256 ± 0.015	0.391TrpTyr: 0.391 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.535TyrAla: 2.535 ± 0.041	0.379TyrCys: 0.379 ± 0.015	1.619TyrAsp: 1.619 ± 0.038	1.127TyrGlu: 1.127 ± 0.028	1.212TyrPhe: 1.212 ± 0.027	2.194TyrGly: 2.194 ± 0.044	0.752TyrHis: 0.752 ± 0.023	1.411TyrIle: 1.411 ± 0.031	0.811TyrLys: 0.811 ± 0.026	3.25TyrLeu: 3.25 ± 0.06	0.551TyrMet: 0.551 ± 0.019	0.902TyrAsn: 0.902 ± 0.027	1.509TyrPro: 1.509 ± 0.035	1.679TyrGln: 1.679 ± 0.03	2.219TyrArg: 2.219 ± 0.041	1.78TyrSer: 1.78 ± 0.036	1.288TyrThr: 1.288 ± 0.027	1.622TyrVal: 1.622 ± 0.033	0.459TyrTrp: 0.459 ± 0.019	0.968TyrTyr: 0.968 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 4605 proteins (1471549 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---