Streptococcus phage P9

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A7J280|A7J280_9CAUD DNA-directed DNA polymerase OS=Streptococcus phage P9 OX=403905 PE=3 SV=1
MM1 pKa = 7.53DD2 pKa = 4.02TEE4 pKa = 4.24EE5 pKa = 4.34HH6 pKa = 6.65PEE8 pKa = 4.08YY9 pKa = 10.13AGHH12 pKa = 6.37MFMSISSKK20 pKa = 9.66TKK22 pKa = 8.84PQIIDD27 pKa = 3.36KK28 pKa = 9.99YY29 pKa = 10.08KK30 pKa = 10.64NSVDD34 pKa = 3.66SAEE37 pKa = 4.01EE38 pKa = 4.46VYY40 pKa = 10.81SGVYY44 pKa = 10.49ARR46 pKa = 11.84VSLNAYY52 pKa = 9.77AYY54 pKa = 7.52NTAGNKK60 pKa = 10.05GISCGLNNVQIVAKK74 pKa = 10.15GDD76 pKa = 3.71YY77 pKa = 10.79LGGRR81 pKa = 11.84SSADD85 pKa = 2.97ADD87 pKa = 3.21FDD89 pKa = 3.64EE90 pKa = 4.81WNEE93 pKa = 4.14EE94 pKa = 3.8EE95 pKa = 6.04DD96 pKa = 4.48EE97 pKa = 5.5DD98 pKa = 6.83DD99 pKa = 4.4ILL101 pKa = 6.16

Molecular weight: 11.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A7J2A1|A7J2A1_9CAUD Uncharacterized protein OS=Streptococcus phage P9 OX=403905 PE=4 SV=1
MM1 pKa = 7.32SLIYY5 pKa = 10.37RR6 pKa = 11.84MRR8 pKa = 11.84GLDD11 pKa = 3.02RR12 pKa = 11.84FLRR15 pKa = 11.84SVEE18 pKa = 4.05RR19 pKa = 11.84KK20 pKa = 8.65QKK22 pKa = 9.38SVRR25 pKa = 11.84IAVDD29 pKa = 3.31KK30 pKa = 10.73EE31 pKa = 4.09LSKK34 pKa = 10.9SAARR38 pKa = 11.84IEE40 pKa = 4.15RR41 pKa = 11.84QAKK44 pKa = 9.19ILAPVDD50 pKa = 3.94TGWLRR55 pKa = 11.84AQIYY59 pKa = 10.15SEE61 pKa = 4.1QQRR64 pKa = 11.84LLHH67 pKa = 5.57YY68 pKa = 9.97RR69 pKa = 11.84VVSPALYY76 pKa = 10.27SIYY79 pKa = 10.79LEE81 pKa = 4.28LGTRR85 pKa = 11.84KK86 pKa = 8.87MEE88 pKa = 4.32AQPFLDD94 pKa = 3.7PALRR98 pKa = 11.84KK99 pKa = 8.48EE100 pKa = 4.46WPVLMANLKK109 pKa = 10.77KK110 pKa = 9.96MFKK113 pKa = 10.29RR114 pKa = 3.74

Molecular weight: 13.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

12344

36

1385

232.9

26.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.036 ± 0.639

0.502 ± 0.096

6.197 ± 0.463

7.259 ± 0.595

3.686 ± 0.208

6.367 ± 0.379

1.28 ± 0.135

7.064 ± 0.336

8.433 ± 0.429

8.765 ± 0.282

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.406 ± 0.143

5.274 ± 0.288

2.754 ± 0.197

3.832 ± 0.264

4.358 ± 0.414

6.578 ± 0.703

6.173 ± 0.313

6.124 ± 0.185

0.972 ± 0.139

3.937 ± 0.399
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski