Amino acid dipepetide frequency for Streptococcus phage P9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.023AlaAla: 5.023 ± 1.052
0.486AlaCys: 0.486 ± 0.155
5.266AlaAsp: 5.266 ± 0.648
6.562AlaGlu: 6.562 ± 0.618
2.836AlaPhe: 2.836 ± 0.735
3.403AlaGly: 3.403 ± 0.746
0.405AlaHis: 0.405 ± 0.205
7.049AlaIle: 7.049 ± 0.832
7.373AlaLys: 7.373 ± 0.875
7.778AlaLeu: 7.778 ± 0.901
2.106AlaMet: 2.106 ± 0.627
5.266AlaAsn: 5.266 ± 1.047
1.62AlaPro: 1.62 ± 0.333
3.727AlaGln: 3.727 ± 0.82
2.998AlaArg: 2.998 ± 0.506
4.78AlaSer: 4.78 ± 1.039
5.509AlaThr: 5.509 ± 1.326
4.78AlaVal: 4.78 ± 1.144
0.324AlaTrp: 0.324 ± 0.176
3.079AlaTyr: 3.079 ± 0.597
0.0AlaXaa: 0.0 ± 0.0
Cys
0.162CysAla: 0.162 ± 0.098
0.081CysCys: 0.081 ± 0.082
0.162CysAsp: 0.162 ± 0.114
0.486CysGlu: 0.486 ± 0.26
0.243CysPhe: 0.243 ± 0.138
0.243CysGly: 0.243 ± 0.139
0.243CysHis: 0.243 ± 0.163
0.324CysIle: 0.324 ± 0.123
0.405CysLys: 0.405 ± 0.163
0.648CysLeu: 0.648 ± 0.288
0.243CysMet: 0.243 ± 0.149
0.162CysAsn: 0.162 ± 0.12
0.081CysPro: 0.081 ± 0.079
0.405CysGln: 0.405 ± 0.17
0.243CysArg: 0.243 ± 0.202
0.324CysSer: 0.324 ± 0.153
0.162CysThr: 0.162 ± 0.141
0.324CysVal: 0.324 ± 0.158
0.081CysTrp: 0.081 ± 0.09
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
3.889AspAla: 3.889 ± 0.509
0.162AspCys: 0.162 ± 0.12
3.322AspAsp: 3.322 ± 0.597
4.942AspGlu: 4.942 ± 0.872
3.241AspPhe: 3.241 ± 0.579
5.023AspGly: 5.023 ± 0.844
0.648AspHis: 0.648 ± 0.25
4.942AspIle: 4.942 ± 0.725
5.59AspLys: 5.59 ± 0.744
5.752AspLeu: 5.752 ± 0.919
1.458AspMet: 1.458 ± 0.334
3.241AspAsn: 3.241 ± 0.583
1.62AspPro: 1.62 ± 0.383
1.215AspGln: 1.215 ± 0.318
2.998AspArg: 2.998 ± 0.649
3.727AspSer: 3.727 ± 0.497
2.998AspThr: 2.998 ± 0.451
3.484AspVal: 3.484 ± 0.602
0.729AspTrp: 0.729 ± 0.262
2.998AspTyr: 2.998 ± 0.511
0.0AspXaa: 0.0 ± 0.0
Glu
5.347GluAla: 5.347 ± 0.813
0.243GluCys: 0.243 ± 0.138
3.322GluAsp: 3.322 ± 0.703
6.481GluGlu: 6.481 ± 0.959
2.187GluPhe: 2.187 ± 0.36
3.322GluGly: 3.322 ± 0.498
1.377GluHis: 1.377 ± 0.426
5.104GluIle: 5.104 ± 0.78
6.481GluLys: 6.481 ± 0.894
7.94GluLeu: 7.94 ± 0.979
1.701GluMet: 1.701 ± 0.364
4.051GluAsn: 4.051 ± 0.722
2.025GluPro: 2.025 ± 0.365
2.593GluGln: 2.593 ± 0.453
3.727GluArg: 3.727 ± 0.856
3.889GluSer: 3.889 ± 0.484
4.051GluThr: 4.051 ± 0.72
3.808GluVal: 3.808 ± 0.619
1.134GluTrp: 1.134 ± 0.258
3.808GluTyr: 3.808 ± 0.706
0.0GluXaa: 0.0 ± 0.0
Phe
3.079PheAla: 3.079 ± 0.894
0.162PheCys: 0.162 ± 0.118
2.593PheAsp: 2.593 ± 0.502
2.025PheGlu: 2.025 ± 0.487
1.296PhePhe: 1.296 ± 0.431
2.998PheGly: 2.998 ± 0.53
0.162PheHis: 0.162 ± 0.11
2.431PheIle: 2.431 ± 0.549
3.16PheLys: 3.16 ± 0.573
2.593PheLeu: 2.593 ± 0.584
0.648PheMet: 0.648 ± 0.232
3.079PheAsn: 3.079 ± 0.426
0.486PhePro: 0.486 ± 0.179
0.486PheGln: 0.486 ± 0.19
1.296PheArg: 1.296 ± 0.294
2.755PheSer: 2.755 ± 0.44
2.674PheThr: 2.674 ± 0.416
2.593PheVal: 2.593 ± 0.401
0.648PheTrp: 0.648 ± 0.287
1.701PheTyr: 1.701 ± 0.464
0.0PheXaa: 0.0 ± 0.0
Gly
5.347GlyAla: 5.347 ± 0.622
0.162GlyCys: 0.162 ± 0.098
3.079GlyAsp: 3.079 ± 0.572
3.889GlyGlu: 3.889 ± 0.535
2.674GlyPhe: 2.674 ± 0.585
4.699GlyGly: 4.699 ± 0.526
1.134GlyHis: 1.134 ± 0.369
4.456GlyIle: 4.456 ± 1.197
5.671GlyLys: 5.671 ± 0.742
6.481GlyLeu: 6.481 ± 0.89
2.106GlyMet: 2.106 ± 0.395
2.106GlyAsn: 2.106 ± 0.475
1.215GlyPro: 1.215 ± 0.431
2.025GlyGln: 2.025 ± 0.389
2.917GlyArg: 2.917 ± 0.476
2.917GlySer: 2.917 ± 0.438
4.699GlyThr: 4.699 ± 0.633
4.78GlyVal: 4.78 ± 0.579
0.729GlyTrp: 0.729 ± 0.327
2.593GlyTyr: 2.593 ± 0.474
0.0GlyXaa: 0.0 ± 0.0
His
1.134HisAla: 1.134 ± 0.299
0.081HisCys: 0.081 ± 0.069
0.486HisAsp: 0.486 ± 0.227
1.053HisGlu: 1.053 ± 0.278
0.486HisPhe: 0.486 ± 0.207
0.567HisGly: 0.567 ± 0.22
0.405HisHis: 0.405 ± 0.188
0.972HisIle: 0.972 ± 0.333
0.729HisLys: 0.729 ± 0.256
0.972HisLeu: 0.972 ± 0.304
0.243HisMet: 0.243 ± 0.138
0.405HisAsn: 0.405 ± 0.167
0.891HisPro: 0.891 ± 0.304
0.324HisGln: 0.324 ± 0.194
0.891HisArg: 0.891 ± 0.237
1.053HisSer: 1.053 ± 0.324
1.053HisThr: 1.053 ± 0.309
0.324HisVal: 0.324 ± 0.14
0.0HisTrp: 0.0 ± 0.0
0.729HisTyr: 0.729 ± 0.314
0.0HisXaa: 0.0 ± 0.0
Ile
5.023IleAla: 5.023 ± 0.884
0.324IleCys: 0.324 ± 0.163
7.292IleAsp: 7.292 ± 0.74
5.266IleGlu: 5.266 ± 1.006
2.106IlePhe: 2.106 ± 0.418
3.646IleGly: 3.646 ± 0.504
0.648IleHis: 0.648 ± 0.251
4.456IleIle: 4.456 ± 0.826
7.616IleLys: 7.616 ± 0.869
4.78IleLeu: 4.78 ± 0.718
1.377IleMet: 1.377 ± 0.33
3.97IleAsn: 3.97 ± 0.579
1.863IlePro: 1.863 ± 0.414
2.187IleGln: 2.187 ± 0.707
2.106IleArg: 2.106 ± 0.414
4.132IleSer: 4.132 ± 0.588
4.78IleThr: 4.78 ± 0.749
4.942IleVal: 4.942 ± 0.681
0.324IleTrp: 0.324 ± 0.163
3.808IleTyr: 3.808 ± 0.607
0.0IleXaa: 0.0 ± 0.0
Lys
6.4LysAla: 6.4 ± 0.749
0.324LysCys: 0.324 ± 0.151
5.671LysAsp: 5.671 ± 0.667
5.509LysGlu: 5.509 ± 0.634
1.701LysPhe: 1.701 ± 0.476
5.59LysGly: 5.59 ± 0.632
1.215LysHis: 1.215 ± 0.387
6.238LysIle: 6.238 ± 0.776
7.211LysLys: 7.211 ± 1.122
7.13LysLeu: 7.13 ± 0.779
2.917LysMet: 2.917 ± 0.524
4.78LysAsn: 4.78 ± 0.759
2.593LysPro: 2.593 ± 0.489
3.646LysGln: 3.646 ± 0.723
4.537LysArg: 4.537 ± 0.742
3.889LysSer: 3.889 ± 0.453
5.752LysThr: 5.752 ± 0.625
5.023LysVal: 5.023 ± 0.732
1.134LysTrp: 1.134 ± 0.353
3.079LysTyr: 3.079 ± 0.576
0.0LysXaa: 0.0 ± 0.0
Leu
6.968LeuAla: 6.968 ± 0.923
0.324LeuCys: 0.324 ± 0.169
6.481LeuAsp: 6.481 ± 0.674
6.805LeuGlu: 6.805 ± 1.203
2.431LeuPhe: 2.431 ± 0.456
5.347LeuGly: 5.347 ± 0.84
0.567LeuHis: 0.567 ± 0.208
4.699LeuIle: 4.699 ± 0.664
8.345LeuLys: 8.345 ± 0.962
6.4LeuLeu: 6.4 ± 0.61
1.782LeuMet: 1.782 ± 0.378
5.023LeuAsn: 5.023 ± 0.575
3.727LeuPro: 3.727 ± 0.746
3.727LeuGln: 3.727 ± 0.577
3.97LeuArg: 3.97 ± 0.636
7.211LeuSer: 7.211 ± 0.893
4.942LeuThr: 4.942 ± 0.597
5.914LeuVal: 5.914 ± 0.58
0.81LeuTrp: 0.81 ± 0.271
2.187LeuTyr: 2.187 ± 0.494
0.0LeuXaa: 0.0 ± 0.0
Met
3.079MetAla: 3.079 ± 0.797
0.081MetCys: 0.081 ± 0.071
0.972MetAsp: 0.972 ± 0.259
1.944MetGlu: 1.944 ± 0.445
1.053MetPhe: 1.053 ± 0.326
1.296MetGly: 1.296 ± 0.31
0.243MetHis: 0.243 ± 0.141
1.863MetIle: 1.863 ± 0.326
0.81MetLys: 0.81 ± 0.253
2.106MetLeu: 2.106 ± 0.42
0.648MetMet: 0.648 ± 0.257
1.296MetAsn: 1.296 ± 0.381
0.891MetPro: 0.891 ± 0.23
0.648MetGln: 0.648 ± 0.215
1.701MetArg: 1.701 ± 0.406
2.674MetSer: 2.674 ± 0.81
1.539MetThr: 1.539 ± 0.374
0.729MetVal: 0.729 ± 0.209
0.081MetTrp: 0.081 ± 0.074
0.405MetTyr: 0.405 ± 0.169
0.0MetXaa: 0.0 ± 0.0
Asn
4.942AsnAla: 4.942 ± 0.646
0.243AsnCys: 0.243 ± 0.141
2.836AsnAsp: 2.836 ± 0.481
3.16AsnGlu: 3.16 ± 0.702
1.944AsnPhe: 1.944 ± 0.495
3.403AsnGly: 3.403 ± 0.596
0.81AsnHis: 0.81 ± 0.274
4.051AsnIle: 4.051 ± 0.484
4.213AsnLys: 4.213 ± 0.608
5.266AsnLeu: 5.266 ± 0.569
0.972AsnMet: 0.972 ± 0.235
2.431AsnAsn: 2.431 ± 0.433
1.782AsnPro: 1.782 ± 0.419
1.701AsnGln: 1.701 ± 0.398
2.025AsnArg: 2.025 ± 0.327
4.456AsnSer: 4.456 ± 0.969
2.836AsnThr: 2.836 ± 0.444
2.512AsnVal: 2.512 ± 0.459
0.648AsnTrp: 0.648 ± 0.193
2.431AsnTyr: 2.431 ± 0.477
0.0AsnXaa: 0.0 ± 0.0
Pro
1.701ProAla: 1.701 ± 0.377
0.405ProCys: 0.405 ± 0.176
1.215ProAsp: 1.215 ± 0.351
2.836ProGlu: 2.836 ± 0.584
0.972ProPhe: 0.972 ± 0.407
2.106ProGly: 2.106 ± 0.47
0.405ProHis: 0.405 ± 0.174
1.701ProIle: 1.701 ± 0.408
1.944ProLys: 1.944 ± 0.451
2.187ProLeu: 2.187 ± 0.36
0.405ProMet: 0.405 ± 0.204
1.377ProAsn: 1.377 ± 0.367
0.648ProPro: 0.648 ± 0.295
1.782ProGln: 1.782 ± 0.447
1.215ProArg: 1.215 ± 0.416
2.187ProSer: 2.187 ± 0.33
1.458ProThr: 1.458 ± 0.298
2.187ProVal: 2.187 ± 0.513
0.243ProTrp: 0.243 ± 0.144
0.567ProTyr: 0.567 ± 0.244
0.0ProXaa: 0.0 ± 0.0
Gln
3.484GlnAla: 3.484 ± 0.56
0.243GlnCys: 0.243 ± 0.14
2.025GlnAsp: 2.025 ± 0.342
2.836GlnGlu: 2.836 ± 0.527
1.377GlnPhe: 1.377 ± 0.348
3.322GlnGly: 3.322 ± 0.575
0.162GlnHis: 0.162 ± 0.128
3.241GlnIle: 3.241 ± 0.652
2.998GlnLys: 2.998 ± 0.553
3.241GlnLeu: 3.241 ± 0.743
1.296GlnMet: 1.296 ± 0.352
2.755GlnAsn: 2.755 ± 0.567
0.486GlnPro: 0.486 ± 0.226
1.215GlnGln: 1.215 ± 0.332
1.215GlnArg: 1.215 ± 0.205
2.836GlnSer: 2.836 ± 0.69
2.35GlnThr: 2.35 ± 0.346
1.458GlnVal: 1.458 ± 0.323
0.405GlnTrp: 0.405 ± 0.186
1.377GlnTyr: 1.377 ± 0.353
0.0GlnXaa: 0.0 ± 0.0
Arg
3.16ArgAla: 3.16 ± 0.503
0.324ArgCys: 0.324 ± 0.154
2.593ArgAsp: 2.593 ± 0.621
2.674ArgGlu: 2.674 ± 0.446
2.755ArgPhe: 2.755 ± 0.439
2.106ArgGly: 2.106 ± 0.331
0.648ArgHis: 0.648 ± 0.226
3.808ArgIle: 3.808 ± 0.431
3.565ArgLys: 3.565 ± 0.565
5.023ArgLeu: 5.023 ± 1.048
1.458ArgMet: 1.458 ± 0.348
2.35ArgAsn: 2.35 ± 0.475
0.891ArgPro: 0.891 ± 0.239
1.458ArgGln: 1.458 ± 0.32
1.539ArgArg: 1.539 ± 0.371
2.025ArgSer: 2.025 ± 0.439
2.674ArgThr: 2.674 ± 0.609
2.512ArgVal: 2.512 ± 0.557
0.486ArgTrp: 0.486 ± 0.204
1.539ArgTyr: 1.539 ± 0.376
0.0ArgXaa: 0.0 ± 0.0
Ser
7.373SerAla: 7.373 ± 2.165
0.405SerCys: 0.405 ± 0.199
3.241SerAsp: 3.241 ± 0.617
4.132SerGlu: 4.132 ± 0.539
2.025SerPhe: 2.025 ± 0.562
5.428SerGly: 5.428 ± 1.076
1.134SerHis: 1.134 ± 0.381
4.861SerIle: 4.861 ± 0.736
5.023SerLys: 5.023 ± 0.497
5.266SerLeu: 5.266 ± 1.551
1.377SerMet: 1.377 ± 0.467
3.16SerAsn: 3.16 ± 0.648
1.458SerPro: 1.458 ± 0.329
2.431SerGln: 2.431 ± 0.552
2.674SerArg: 2.674 ± 0.52
5.185SerSer: 5.185 ± 1.477
2.998SerThr: 2.998 ± 0.711
4.78SerVal: 4.78 ± 0.718
0.567SerTrp: 0.567 ± 0.224
2.268SerTyr: 2.268 ± 0.404
0.0SerXaa: 0.0 ± 0.0
Thr
5.266ThrAla: 5.266 ± 0.904
0.243ThrCys: 0.243 ± 0.182
3.97ThrAsp: 3.97 ± 0.667
3.322ThrGlu: 3.322 ± 0.544
2.917ThrPhe: 2.917 ± 0.439
5.347ThrGly: 5.347 ± 0.645
0.648ThrHis: 0.648 ± 0.216
3.646ThrIle: 3.646 ± 0.416
4.618ThrLys: 4.618 ± 0.564
5.104ThrLeu: 5.104 ± 0.728
1.215ThrMet: 1.215 ± 0.294
1.863ThrAsn: 1.863 ± 0.325
1.701ThrPro: 1.701 ± 0.398
2.917ThrGln: 2.917 ± 0.457
2.268ThrArg: 2.268 ± 0.407
4.294ThrSer: 4.294 ± 1.82
4.78ThrThr: 4.78 ± 0.782
4.213ThrVal: 4.213 ± 0.588
0.81ThrTrp: 0.81 ± 0.406
2.593ThrTyr: 2.593 ± 0.567
0.0ThrXaa: 0.0 ± 0.0
Val
5.185ValAla: 5.185 ± 0.561
0.243ValCys: 0.243 ± 0.142
4.051ValAsp: 4.051 ± 0.574
4.537ValGlu: 4.537 ± 0.721
2.35ValPhe: 2.35 ± 0.402
3.484ValGly: 3.484 ± 0.6
1.053ValHis: 1.053 ± 0.247
4.294ValIle: 4.294 ± 0.713
4.375ValLys: 4.375 ± 0.531
4.78ValLeu: 4.78 ± 0.618
1.62ValMet: 1.62 ± 0.373
3.079ValAsn: 3.079 ± 0.431
1.701ValPro: 1.701 ± 0.483
2.755ValGln: 2.755 ± 0.875
2.431ValArg: 2.431 ± 0.437
4.375ValSer: 4.375 ± 0.854
3.97ValThr: 3.97 ± 0.579
4.132ValVal: 4.132 ± 0.49
0.162ValTrp: 0.162 ± 0.128
2.674ValTyr: 2.674 ± 0.52
0.0ValXaa: 0.0 ± 0.0
Trp
0.567TrpAla: 0.567 ± 0.203
0.0TrpCys: 0.0 ± 0.0
0.972TrpAsp: 0.972 ± 0.241
1.053TrpGlu: 1.053 ± 0.211
0.567TrpPhe: 0.567 ± 0.225
0.567TrpGly: 0.567 ± 0.234
0.162TrpHis: 0.162 ± 0.12
0.729TrpIle: 0.729 ± 0.192
0.81TrpLys: 0.81 ± 0.236
0.891TrpLeu: 0.891 ± 0.274
0.0TrpMet: 0.0 ± 0.0
0.324TrpAsn: 0.324 ± 0.159
0.648TrpPro: 0.648 ± 0.342
0.567TrpGln: 0.567 ± 0.226
0.486TrpArg: 0.486 ± 0.266
0.486TrpSer: 0.486 ± 0.204
0.243TrpThr: 0.243 ± 0.153
0.486TrpVal: 0.486 ± 0.276
0.0TrpTrp: 0.0 ± 0.0
0.162TrpTyr: 0.162 ± 0.115
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.241TyrAla: 3.241 ± 0.488
0.486TyrCys: 0.486 ± 0.216
2.431TyrAsp: 2.431 ± 0.541
3.079TyrGlu: 3.079 ± 0.556
1.701TyrPhe: 1.701 ± 0.377
1.782TyrGly: 1.782 ± 0.432
0.729TyrHis: 0.729 ± 0.234
1.782TyrIle: 1.782 ± 0.406
3.403TyrLys: 3.403 ± 0.697
3.322TyrLeu: 3.322 ± 0.676
0.405TyrMet: 0.405 ± 0.157
2.025TyrAsn: 2.025 ± 0.43
1.215TyrPro: 1.215 ± 0.353
2.512TyrGln: 2.512 ± 0.544
2.35TyrArg: 2.35 ± 0.61
2.593TyrSer: 2.593 ± 0.416
2.268TyrThr: 2.268 ± 0.465
2.268TyrVal: 2.268 ± 0.534
0.405TyrTrp: 0.405 ± 0.161
1.377TyrTyr: 1.377 ± 0.335
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 53 proteins (12344 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski