Lysinibacillus manganicus DSM 26584

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Planococcaceae; Ureibacillus; Ureibacillus manganicus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3553 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A3I1K7|A0A0A3I1K7_9BACI Membrane protein OS=Lysinibacillus manganicus DSM 26584 OX=1384049 GN=CD29_10090 PE=4 SV=1
MM1 pKa = 7.45VHH3 pKa = 6.02EE4 pKa = 5.32HH5 pKa = 6.7DD6 pKa = 4.24HH7 pKa = 5.95DD8 pKa = 4.09HH9 pKa = 6.12RR10 pKa = 11.84HH11 pKa = 4.67ITIVDD16 pKa = 3.37EE17 pKa = 5.17DD18 pKa = 4.39GNEE21 pKa = 3.91QLCEE25 pKa = 3.94VIYY28 pKa = 10.19TFDD31 pKa = 3.42SEE33 pKa = 4.92EE34 pKa = 3.91FGKK37 pKa = 10.83SYY39 pKa = 11.29VLYY42 pKa = 9.94SLVGAEE48 pKa = 4.69EE49 pKa = 4.12DD50 pKa = 3.63AEE52 pKa = 4.28GQIEE56 pKa = 4.21IFASAFTPSEE66 pKa = 3.92NGEE69 pKa = 4.23DD70 pKa = 4.31GEE72 pKa = 4.56LEE74 pKa = 4.52PIEE77 pKa = 5.16TEE79 pKa = 4.37AEE81 pKa = 3.43WDD83 pKa = 4.14LIEE86 pKa = 4.94EE87 pKa = 4.34VLNTLEE93 pKa = 6.05DD94 pKa = 3.81EE95 pKa = 5.2LDD97 pKa = 4.45DD98 pKa = 4.56EE99 pKa = 5.66DD100 pKa = 5.33EE101 pKa = 4.27EE102 pKa = 4.62

Molecular weight: 11.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A3I8V5|A0A0A3I8V5_9BACI Endoribonuclease L-PSP OS=Lysinibacillus manganicus DSM 26584 OX=1384049 GN=CD29_07600 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.54QPKK8 pKa = 9.44KK9 pKa = 7.96RR10 pKa = 11.84KK11 pKa = 8.69HH12 pKa = 5.94SKK14 pKa = 8.54VHH16 pKa = 5.68GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.2NGRR28 pKa = 11.84KK29 pKa = 9.39VIAARR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.04KK37 pKa = 9.84GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3553

0

3553

1035309

37

1504

291.4

32.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.615 ± 0.042

0.695 ± 0.012

5.024 ± 0.033

7.581 ± 0.043

4.756 ± 0.037

6.614 ± 0.037

2.004 ± 0.021

8.55 ± 0.049

6.774 ± 0.036

9.725 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.617 ± 0.02

4.901 ± 0.034

3.438 ± 0.025

3.656 ± 0.027

3.768 ± 0.031

6.048 ± 0.035

5.575 ± 0.025

7.138 ± 0.031

0.941 ± 0.013

3.58 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski