Acinetobacter phage AbTZA1
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 253 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3Q9R7C6|A0A3Q9R7C6_9CAUD Uncharacterized protein OS=Acinetobacter phage AbTZA1 OX=2500827 PE=4 SV=1
MM1 pKa = 7.13 FAAIHH6 pKa = 6.22 LVTEE10 pKa = 4.54 SSDD13 pKa = 3.09 HH14 pKa = 5.5 YY15 pKa = 11.24 NYY17 pKa = 10.41 LIEE20 pKa = 4.27 YY21 pKa = 6.47 TTVEE25 pKa = 4.21 DD26 pKa = 5.23 VINEE30 pKa = 4.03 LKK32 pKa = 11.11 GLDD35 pKa = 3.72 TEE37 pKa = 4.83 LGCVSDD43 pKa = 4.97 YY44 pKa = 11.49 YY45 pKa = 11.48 VDD47 pKa = 3.78 CQEE50 pKa = 4.46 SFKK53 pKa = 11.22 DD54 pKa = 3.59 DD55 pKa = 3.21 EE56 pKa = 4.58 RR57 pKa = 11.84 KK58 pKa = 9.69 IEE60 pKa = 4.04 EE61 pKa = 4.6 LLRR64 pKa = 11.84 DD65 pKa = 4.43 AIDD68 pKa = 3.63 SQSAANEE75 pKa = 3.63 AAYY78 pKa = 10.54 FEE80 pKa = 5.49 AYY82 pKa = 9.98 AA83 pKa = 4.63
Molecular weight: 9.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.845
IPC2_protein 3.961
IPC_protein 3.884
Toseland 3.694
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.757
Rodwell 3.719
Grimsley 3.605
Solomon 3.846
Lehninger 3.795
Nozaki 3.973
DTASelect 4.139
Thurlkill 3.745
EMBOSS 3.77
Sillero 3.999
Patrickios 0.426
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.894
Protein with the highest isoelectric point:
>tr|A0A3Q9R781|A0A3Q9R781_9CAUD Uncharacterized protein OS=Acinetobacter phage AbTZA1 OX=2500827 PE=4 SV=1
MM1 pKa = 7.78 IGIVILLIVLLGALFRR17 pKa = 11.84 RR18 pKa = 11.84 NRR20 pKa = 11.84 QQARR24 pKa = 11.84 AISKK28 pKa = 9.87 LKK30 pKa = 10.75 YY31 pKa = 10.26 DD32 pKa = 3.84 VFCGKK37 pKa = 10.42 LKK39 pKa = 10.46 FYY41 pKa = 10.45 RR42 pKa = 11.84 AKK44 pKa = 11.03 NEE46 pKa = 3.73 LNRR49 pKa = 11.84 IKK51 pKa = 10.94 NPLPDD56 pKa = 3.85 LSSLKK61 pKa = 10.38 KK62 pKa = 10.75 LEE64 pKa = 4.73 FIGFDD69 pKa = 3.49 KK70 pKa = 10.87 IALNFFNRR78 pKa = 3.61
Molecular weight: 9.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.292
IPC2_protein 9.809
IPC_protein 10.262
Toseland 10.891
ProMoST 10.701
Dawson 10.95
Bjellqvist 10.599
Wikipedia 11.111
Rodwell 11.403
Grimsley 10.979
Solomon 11.038
Lehninger 11.023
Nozaki 10.862
DTASelect 10.584
Thurlkill 10.862
EMBOSS 11.272
Sillero 10.877
Patrickios 11.155
IPC_peptide 11.052
IPC2_peptide 9.311
IPC2.peptide.svr19 8.694
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
253
0
253
52344
38
1380
206.9
23.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.088 ± 0.174
1.07 ± 0.066
6.184 ± 0.125
6.73 ± 0.189
4.516 ± 0.103
5.796 ± 0.176
1.928 ± 0.083
7.506 ± 0.137
7.151 ± 0.157
7.917 ± 0.146
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.552 ± 0.079
5.716 ± 0.116
3.605 ± 0.107
3.452 ± 0.086
4.234 ± 0.092
6.4 ± 0.136
6.197 ± 0.25
6.604 ± 0.117
1.305 ± 0.056
4.048 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here