Scopulibacillus darangshiensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Sporolactobacillaceae; Scopulibacillus

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4593 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V2SMX5|A0A4V2SMX5_9BACL Methylisocitrate lyase OS=Scopulibacillus darangshiensis OX=442528 GN=EV207_11340 PE=3 SV=1
MM1 pKa = 7.39LNVAFGAVNSKK12 pKa = 10.76NMTTTCSIVFGEE24 pKa = 4.11NNQVGWSAHH33 pKa = 4.74GKK35 pKa = 9.54FNYY38 pKa = 10.03ANGWLYY44 pKa = 10.85GVSLNTGVFNNMIDD58 pKa = 3.64NDD60 pKa = 4.24VIDD63 pKa = 4.81TPIQDD68 pKa = 4.12DD69 pKa = 4.11DD70 pKa = 4.73GVPSSQTQGLL80 pKa = 3.76

Molecular weight: 8.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R2NG26|A0A4R2NG26_9BACL Pyridine nucleotide-disulfide oxidoreductase OS=Scopulibacillus darangshiensis OX=442528 GN=EV207_1542 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.36QPNTRR10 pKa = 11.84KK11 pKa = 9.75RR12 pKa = 11.84KK13 pKa = 8.32KK14 pKa = 8.57VHH16 pKa = 5.6GFRR19 pKa = 11.84SRR21 pKa = 11.84MQTKK25 pKa = 10.07NGRR28 pKa = 11.84KK29 pKa = 8.31VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4593

0

4593

1339328

25

1910

291.6

32.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.327 ± 0.035

0.81 ± 0.011

5.511 ± 0.035

6.581 ± 0.045

4.458 ± 0.035

7.289 ± 0.035

2.27 ± 0.018

7.735 ± 0.038

7.122 ± 0.041

9.613 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.807 ± 0.017

4.344 ± 0.026

3.804 ± 0.019

3.522 ± 0.024

4.176 ± 0.024

5.925 ± 0.025

5.292 ± 0.021

6.881 ± 0.028

1.096 ± 0.015

3.439 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski