Streptococcus phage Javan384
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6B3R1|A0A4D6B3R1_9CAUD Uncharacterized protein OS=Streptococcus phage Javan384 OX=2548140 GN=Javan384_0051 PE=4 SV=1
MM1 pKa = 7.81 IINIVDD7 pKa = 3.73 YY8 pKa = 10.67 KK9 pKa = 11.02 DD10 pKa = 4.03 DD11 pKa = 5.43 RR12 pKa = 11.84 IPVYY16 pKa = 10.41 IGDD19 pKa = 4.24 IEE21 pKa = 4.58 NIYY24 pKa = 9.37 YY25 pKa = 9.81 IQVEE29 pKa = 4.51 VLSGDD34 pKa = 3.54 EE35 pKa = 5.4 VITVWYY41 pKa = 10.38 KK42 pKa = 11.0 DD43 pKa = 3.32 GTYY46 pKa = 10.62 KK47 pKa = 10.91 DD48 pKa = 5.25 DD49 pKa = 5.35 LDD51 pKa = 4.48 SSQCRR56 pKa = 11.84 LADD59 pKa = 3.89 YY60 pKa = 11.2 YY61 pKa = 11.41 DD62 pKa = 3.87 GDD64 pKa = 4.18 YY65 pKa = 10.71 EE66 pKa = 4.54 VPSEE70 pKa = 5.17 QIEE73 pKa = 4.35 KK74 pKa = 8.66 WSNMPGSSYY83 pKa = 11.36 DD84 pKa = 3.2 QMEE87 pKa = 4.05 RR88 pKa = 11.84 FIEE91 pKa = 4.39 SVEE94 pKa = 4.06 EE95 pKa = 3.98
Molecular weight: 11.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.784
IPC2_protein 3.757
IPC_protein 3.732
Toseland 3.516
ProMoST 3.859
Dawson 3.719
Bjellqvist 3.923
Wikipedia 3.656
Rodwell 3.554
Grimsley 3.427
Solomon 3.706
Lehninger 3.656
Nozaki 3.846
DTASelect 4.05
Thurlkill 3.579
EMBOSS 3.668
Sillero 3.846
Patrickios 0.426
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.8
Protein with the highest isoelectric point:
>tr|A0A4D6B2K1|A0A4D6B2K1_9CAUD Uncharacterized protein OS=Streptococcus phage Javan384 OX=2548140 GN=Javan384_0024 PE=4 SV=1
MM1 pKa = 7.59 KK2 pKa = 9.34 FTVKK6 pKa = 9.38 GTEE9 pKa = 3.8 KK10 pKa = 10.65 LEE12 pKa = 3.89 EE13 pKa = 4.06 RR14 pKa = 11.84 FRR16 pKa = 11.84 AMNSIRR22 pKa = 11.84 WDD24 pKa = 3.55 GVVNKK29 pKa = 10.52 SLTQMFNRR37 pKa = 11.84 GARR40 pKa = 11.84 PPGTPVGKK48 pKa = 7.82 QTKK51 pKa = 8.21 NHH53 pKa = 6.19 SAGEE57 pKa = 4.07 LRR59 pKa = 11.84 RR60 pKa = 11.84 SRR62 pKa = 11.84 RR63 pKa = 11.84 VKK65 pKa = 10.46 KK66 pKa = 10.46 ISSKK70 pKa = 10.54 DD71 pKa = 3.34 GSYY74 pKa = 9.97 TGSFYY79 pKa = 9.74 YY80 pKa = 10.33 TKK82 pKa = 10.39 DD83 pKa = 3.48 YY84 pKa = 10.82 APHH87 pKa = 6.47 VEE89 pKa = 4.05 YY90 pKa = 10.64 GHH92 pKa = 6.97 RR93 pKa = 11.84 IVRR96 pKa = 11.84 KK97 pKa = 8.15 GKK99 pKa = 9.36 QVGYY103 pKa = 11.11 VNGKK107 pKa = 9.81 KK108 pKa = 10.37 YY109 pKa = 10.92 LFNNVQKK116 pKa = 10.47 QRR118 pKa = 11.84 QIYY121 pKa = 8.74 FKK123 pKa = 11.51 DD124 pKa = 3.12 MLAEE128 pKa = 3.99 LRR130 pKa = 11.84 KK131 pKa = 10.03 KK132 pKa = 10.47 RR133 pKa = 3.7
Molecular weight: 15.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.287
IPC2_protein 10.014
IPC_protein 10.584
Toseland 10.818
ProMoST 10.526
Dawson 10.921
Bjellqvist 10.57
Wikipedia 11.082
Rodwell 11.359
Grimsley 10.965
Solomon 10.979
Lehninger 10.965
Nozaki 10.774
DTASelect 10.57
Thurlkill 10.804
EMBOSS 11.199
Sillero 10.833
Patrickios 11.067
IPC_peptide 10.994
IPC2_peptide 9.238
IPC2.peptide.svr19 8.446
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
64
0
64
11161
37
983
174.4
19.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.994 ± 0.37
0.672 ± 0.099
6.102 ± 0.345
7.544 ± 0.496
4.22 ± 0.2
6.344 ± 0.51
1.416 ± 0.116
7.956 ± 0.347
8.897 ± 0.4
8.162 ± 0.291
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.428 ± 0.192
6.397 ± 0.253
2.643 ± 0.14
3.351 ± 0.186
3.665 ± 0.261
6.272 ± 0.379
6.29 ± 0.358
5.976 ± 0.206
1.201 ± 0.122
4.471 ± 0.262
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here