Sphingomonas phage Lucius

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3TC43|A0A6M3TC43_9CAUD Uncharacterized protein OS=Sphingomonas phage Lucius OX=2686313 PE=4 SV=1
MM1 pKa = 7.7AYY3 pKa = 9.85EE4 pKa = 4.73HH5 pKa = 7.21DD6 pKa = 3.98TTVLGGLPVTIEE18 pKa = 3.96YY19 pKa = 10.14SVQGAEE25 pKa = 3.9PDD27 pKa = 3.62VGIMSAYY34 pKa = 8.83VDD36 pKa = 3.22EE37 pKa = 4.8WYY39 pKa = 10.0IVAINGRR46 pKa = 11.84AVKK49 pKa = 10.46KK50 pKa = 10.65CDD52 pKa = 3.22WLDD55 pKa = 3.29KK56 pKa = 10.74RR57 pKa = 11.84IAEE60 pKa = 4.6TKK62 pKa = 10.72GEE64 pKa = 3.87TDD66 pKa = 4.31RR67 pKa = 11.84IVEE70 pKa = 4.35EE71 pKa = 4.17LNEE74 pKa = 4.22AASEE78 pKa = 3.98ADD80 pKa = 3.43YY81 pKa = 10.86DD82 pKa = 4.2YY83 pKa = 11.42PDD85 pKa = 3.51YY86 pKa = 11.65

Molecular weight: 9.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3T9W9|A0A6M3T9W9_9CAUD Uncharacterized protein OS=Sphingomonas phage Lucius OX=2686313 PE=4 SV=1
MM1 pKa = 7.52KK2 pKa = 10.22GHH4 pKa = 6.2VMQLVVDD11 pKa = 4.5NAAPLPRR18 pKa = 11.84RR19 pKa = 11.84TASYY23 pKa = 11.1SEE25 pKa = 3.68AAYY28 pKa = 10.44FDD30 pKa = 5.2GEE32 pKa = 4.51HH33 pKa = 6.4CQHH36 pKa = 6.28IADD39 pKa = 3.89WEE41 pKa = 4.26EE42 pKa = 3.46QRR44 pKa = 11.84RR45 pKa = 11.84LQRR48 pKa = 11.84IRR50 pKa = 11.84TSAIKK55 pKa = 10.53ALDD58 pKa = 3.45RR59 pKa = 11.84RR60 pKa = 11.84SVRR63 pKa = 11.84RR64 pKa = 3.47

Molecular weight: 7.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

13997

35

961

233.3

25.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.817 ± 0.57

1.072 ± 0.162

6.451 ± 0.245

5.916 ± 0.265

4.222 ± 0.199

8.48 ± 0.44

1.657 ± 0.157

4.858 ± 0.163

5.151 ± 0.329

7.059 ± 0.193

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.579 ± 0.156

4.73 ± 0.217

4.844 ± 0.326

3.386 ± 0.326

5.501 ± 0.277

5.344 ± 0.229

6.073 ± 0.314

7.244 ± 0.286

1.643 ± 0.158

2.972 ± 0.191
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski