Amino acid dipepetide frequency for Sphingomonas phage Lucius

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.289AlaAla: 12.289 ± 1.7
0.714AlaCys: 0.714 ± 0.255
6.43AlaAsp: 6.43 ± 0.68
5.716AlaGlu: 5.716 ± 0.777
4.215AlaPhe: 4.215 ± 0.522
9.36AlaGly: 9.36 ± 0.819
2.215AlaHis: 2.215 ± 0.423
6.145AlaIle: 6.145 ± 0.912
6.931AlaLys: 6.931 ± 0.863
8.217AlaLeu: 8.217 ± 0.811
3.43AlaMet: 3.43 ± 0.459
5.359AlaAsn: 5.359 ± 0.683
5.001AlaPro: 5.001 ± 0.667
4.43AlaGln: 4.43 ± 0.885
3.93AlaArg: 3.93 ± 0.653
6.216AlaSer: 6.216 ± 0.64
7.002AlaThr: 7.002 ± 0.844
6.502AlaVal: 6.502 ± 0.721
1.358AlaTrp: 1.358 ± 0.267
2.715AlaTyr: 2.715 ± 0.358
0.0AlaXaa: 0.0 ± 0.0
Cys
0.643CysAla: 0.643 ± 0.23
0.143CysCys: 0.143 ± 0.104
0.786CysAsp: 0.786 ± 0.279
0.357CysGlu: 0.357 ± 0.134
0.786CysPhe: 0.786 ± 0.245
0.929CysGly: 0.929 ± 0.301
0.643CysHis: 0.643 ± 0.234
0.357CysIle: 0.357 ± 0.177
0.5CysLys: 0.5 ± 0.187
0.5CysLeu: 0.5 ± 0.192
0.143CysMet: 0.143 ± 0.1
0.857CysAsn: 0.857 ± 0.265
0.786CysPro: 0.786 ± 0.214
0.429CysGln: 0.429 ± 0.179
0.857CysArg: 0.857 ± 0.329
0.429CysSer: 0.429 ± 0.178
0.357CysThr: 0.357 ± 0.226
0.643CysVal: 0.643 ± 0.265
0.143CysTrp: 0.143 ± 0.105
0.429CysTyr: 0.429 ± 0.177
0.0CysXaa: 0.0 ± 0.0
Asp
7.073AspAla: 7.073 ± 0.72
0.5AspCys: 0.5 ± 0.17
4.716AspAsp: 4.716 ± 0.666
5.502AspGlu: 5.502 ± 0.474
2.858AspPhe: 2.858 ± 0.5
5.644AspGly: 5.644 ± 0.693
0.786AspHis: 0.786 ± 0.244
2.429AspIle: 2.429 ± 0.331
3.715AspLys: 3.715 ± 0.583
4.358AspLeu: 4.358 ± 0.651
1.858AspMet: 1.858 ± 0.421
2.787AspAsn: 2.787 ± 0.402
3.787AspPro: 3.787 ± 0.494
1.072AspGln: 1.072 ± 0.293
3.43AspArg: 3.43 ± 0.443
1.858AspSer: 1.858 ± 0.346
3.715AspThr: 3.715 ± 0.479
4.716AspVal: 4.716 ± 0.613
1.072AspTrp: 1.072 ± 0.318
2.572AspTyr: 2.572 ± 0.456
0.0AspXaa: 0.0 ± 0.0
Glu
7.145GluAla: 7.145 ± 0.818
1.072GluCys: 1.072 ± 0.34
3.572GluAsp: 3.572 ± 0.468
2.787GluGlu: 2.787 ± 0.514
1.929GluPhe: 1.929 ± 0.341
4.644GluGly: 4.644 ± 0.668
1.429GluHis: 1.429 ± 0.39
2.715GluIle: 2.715 ± 0.422
2.429GluLys: 2.429 ± 0.346
5.001GluLeu: 5.001 ± 0.567
1.429GluMet: 1.429 ± 0.327
1.643GluAsn: 1.643 ± 0.328
2.501GluPro: 2.501 ± 0.316
2.715GluGln: 2.715 ± 0.415
4.287GluArg: 4.287 ± 0.67
1.358GluSer: 1.358 ± 0.249
4.001GluThr: 4.001 ± 0.605
4.716GluVal: 4.716 ± 0.419
1.429GluTrp: 1.429 ± 0.337
2.358GluTyr: 2.358 ± 0.492
0.0GluXaa: 0.0 ± 0.0
Phe
4.573PheAla: 4.573 ± 0.621
0.214PheCys: 0.214 ± 0.11
4.144PheAsp: 4.144 ± 0.526
2.644PheGlu: 2.644 ± 0.504
1.786PhePhe: 1.786 ± 0.371
3.858PheGly: 3.858 ± 0.648
0.714PheHis: 0.714 ± 0.201
2.143PheIle: 2.143 ± 0.352
1.715PheLys: 1.715 ± 0.341
1.858PheLeu: 1.858 ± 0.337
0.929PheMet: 0.929 ± 0.267
2.572PheAsn: 2.572 ± 0.318
1.429PhePro: 1.429 ± 0.393
0.929PheGln: 0.929 ± 0.245
2.358PheArg: 2.358 ± 0.508
2.215PheSer: 2.215 ± 0.348
2.858PheThr: 2.858 ± 0.528
3.43PheVal: 3.43 ± 0.562
0.857PheTrp: 0.857 ± 0.258
1.0PheTyr: 1.0 ± 0.265
0.0PheXaa: 0.0 ± 0.0
Gly
6.788GlyAla: 6.788 ± 0.632
0.643GlyCys: 0.643 ± 0.22
5.93GlyAsp: 5.93 ± 0.577
6.002GlyGlu: 6.002 ± 0.845
3.715GlyPhe: 3.715 ± 0.526
9.003GlyGly: 9.003 ± 2.479
1.358GlyHis: 1.358 ± 0.35
3.358GlyIle: 3.358 ± 0.475
4.501GlyLys: 4.501 ± 0.594
6.859GlyLeu: 6.859 ± 0.694
1.572GlyMet: 1.572 ± 0.309
3.43GlyAsn: 3.43 ± 0.796
2.787GlyPro: 2.787 ± 0.491
4.287GlyGln: 4.287 ± 0.789
4.287GlyArg: 4.287 ± 0.597
4.573GlySer: 4.573 ± 0.684
4.573GlyThr: 4.573 ± 0.725
7.359GlyVal: 7.359 ± 0.896
1.643GlyTrp: 1.643 ± 0.423
2.143GlyTyr: 2.143 ± 0.424
0.0GlyXaa: 0.0 ± 0.0
His
0.857HisAla: 0.857 ± 0.241
0.357HisCys: 0.357 ± 0.145
1.215HisAsp: 1.215 ± 0.368
0.572HisGlu: 0.572 ± 0.223
0.857HisPhe: 0.857 ± 0.27
1.643HisGly: 1.643 ± 0.362
0.143HisHis: 0.143 ± 0.092
1.143HisIle: 1.143 ± 0.235
0.429HisLys: 0.429 ± 0.18
1.215HisLeu: 1.215 ± 0.331
0.429HisMet: 0.429 ± 0.194
1.072HisAsn: 1.072 ± 0.285
1.5HisPro: 1.5 ± 0.368
0.143HisGln: 0.143 ± 0.097
0.929HisArg: 0.929 ± 0.335
0.929HisSer: 0.929 ± 0.205
0.786HisThr: 0.786 ± 0.195
1.572HisVal: 1.572 ± 0.337
0.143HisTrp: 0.143 ± 0.124
0.643HisTyr: 0.643 ± 0.296
0.0HisXaa: 0.0 ± 0.0
Ile
7.073IleAla: 7.073 ± 0.742
0.429IleCys: 0.429 ± 0.177
4.144IleAsp: 4.144 ± 0.559
3.787IleGlu: 3.787 ± 0.494
1.715IlePhe: 1.715 ± 0.373
3.858IleGly: 3.858 ± 0.502
0.643IleHis: 0.643 ± 0.194
2.429IleIle: 2.429 ± 0.393
2.429IleLys: 2.429 ± 0.395
1.643IleLeu: 1.643 ± 0.288
1.215IleMet: 1.215 ± 0.402
3.43IleAsn: 3.43 ± 0.519
1.715IlePro: 1.715 ± 0.396
1.286IleGln: 1.286 ± 0.311
2.072IleArg: 2.072 ± 0.379
2.429IleSer: 2.429 ± 0.384
2.787IleThr: 2.787 ± 0.465
4.001IleVal: 4.001 ± 0.623
0.143IleTrp: 0.143 ± 0.093
1.358IleTyr: 1.358 ± 0.251
0.0IleXaa: 0.0 ± 0.0
Lys
6.002LysAla: 6.002 ± 0.747
0.5LysCys: 0.5 ± 0.204
2.072LysAsp: 2.072 ± 0.394
3.072LysGlu: 3.072 ± 0.652
2.358LysPhe: 2.358 ± 0.318
3.644LysGly: 3.644 ± 0.603
0.857LysHis: 0.857 ± 0.239
2.715LysIle: 2.715 ± 0.436
2.501LysLys: 2.501 ± 0.449
4.001LysLeu: 4.001 ± 0.729
1.643LysMet: 1.643 ± 0.298
2.001LysAsn: 2.001 ± 0.403
3.072LysPro: 3.072 ± 0.522
1.358LysGln: 1.358 ± 0.333
3.715LysArg: 3.715 ± 0.549
3.358LysSer: 3.358 ± 0.492
3.572LysThr: 3.572 ± 0.556
2.501LysVal: 2.501 ± 0.405
1.0LysTrp: 1.0 ± 0.332
1.572LysTyr: 1.572 ± 0.304
0.0LysXaa: 0.0 ± 0.0
Leu
7.574LeuAla: 7.574 ± 0.855
0.429LeuCys: 0.429 ± 0.167
4.144LeuAsp: 4.144 ± 0.531
3.287LeuGlu: 3.287 ± 0.538
3.001LeuPhe: 3.001 ± 0.634
5.287LeuGly: 5.287 ± 0.525
1.286LeuHis: 1.286 ± 0.307
3.072LeuIle: 3.072 ± 0.529
4.001LeuLys: 4.001 ± 0.606
4.43LeuLeu: 4.43 ± 0.511
1.286LeuMet: 1.286 ± 0.245
3.572LeuAsn: 3.572 ± 0.421
4.43LeuPro: 4.43 ± 0.692
2.358LeuGln: 2.358 ± 0.472
4.787LeuArg: 4.787 ± 0.55
4.287LeuSer: 4.287 ± 0.556
4.644LeuThr: 4.644 ± 0.593
5.644LeuVal: 5.644 ± 0.717
1.286LeuTrp: 1.286 ± 0.349
1.786LeuTyr: 1.786 ± 0.331
0.0LeuXaa: 0.0 ± 0.0
Met
2.929MetAla: 2.929 ± 0.449
0.286MetCys: 0.286 ± 0.141
1.358MetAsp: 1.358 ± 0.285
1.358MetGlu: 1.358 ± 0.316
0.929MetPhe: 0.929 ± 0.273
1.786MetGly: 1.786 ± 0.338
0.143MetHis: 0.143 ± 0.099
0.786MetIle: 0.786 ± 0.295
1.5MetLys: 1.5 ± 0.322
2.286MetLeu: 2.286 ± 0.428
0.857MetMet: 0.857 ± 0.273
1.429MetAsn: 1.429 ± 0.333
1.643MetPro: 1.643 ± 0.373
0.857MetGln: 0.857 ± 0.266
1.215MetArg: 1.215 ± 0.314
1.572MetSer: 1.572 ± 0.344
2.501MetThr: 2.501 ± 0.479
1.643MetVal: 1.643 ± 0.329
0.357MetTrp: 0.357 ± 0.144
0.357MetTyr: 0.357 ± 0.148
0.0MetXaa: 0.0 ± 0.0
Asn
5.93AsnAla: 5.93 ± 0.823
0.357AsnCys: 0.357 ± 0.156
3.572AsnAsp: 3.572 ± 0.574
2.787AsnGlu: 2.787 ± 0.362
1.072AsnPhe: 1.072 ± 0.226
5.43AsnGly: 5.43 ± 0.825
1.0AsnHis: 1.0 ± 0.244
1.429AsnIle: 1.429 ± 0.352
2.215AsnLys: 2.215 ± 0.418
2.286AsnLeu: 2.286 ± 0.333
1.5AsnMet: 1.5 ± 0.39
2.215AsnAsn: 2.215 ± 0.462
2.787AsnPro: 2.787 ± 0.404
1.215AsnGln: 1.215 ± 0.226
1.858AsnArg: 1.858 ± 0.387
3.144AsnSer: 3.144 ± 0.378
2.215AsnThr: 2.215 ± 0.408
4.716AsnVal: 4.716 ± 0.56
0.572AsnTrp: 0.572 ± 0.213
1.0AsnTyr: 1.0 ± 0.287
0.0AsnXaa: 0.0 ± 0.0
Pro
5.359ProAla: 5.359 ± 0.727
0.572ProCys: 0.572 ± 0.206
3.072ProAsp: 3.072 ± 0.532
3.43ProGlu: 3.43 ± 0.414
2.072ProPhe: 2.072 ± 0.473
3.858ProGly: 3.858 ± 0.663
0.857ProHis: 0.857 ± 0.258
2.286ProIle: 2.286 ± 0.451
3.072ProLys: 3.072 ± 0.45
3.287ProLeu: 3.287 ± 0.499
1.072ProMet: 1.072 ± 0.36
2.644ProAsn: 2.644 ± 0.484
3.072ProPro: 3.072 ± 0.837
1.0ProGln: 1.0 ± 0.264
2.215ProArg: 2.215 ± 0.566
2.501ProSer: 2.501 ± 0.34
3.001ProThr: 3.001 ± 0.483
3.215ProVal: 3.215 ± 0.534
0.5ProTrp: 0.5 ± 0.216
1.358ProTyr: 1.358 ± 0.377
0.0ProXaa: 0.0 ± 0.0
Gln
4.215GlnAla: 4.215 ± 0.789
0.5GlnCys: 0.5 ± 0.162
0.786GlnAsp: 0.786 ± 0.206
1.072GlnGlu: 1.072 ± 0.27
1.358GlnPhe: 1.358 ± 0.28
2.501GlnGly: 2.501 ± 0.749
0.857GlnHis: 0.857 ± 0.258
1.5GlnIle: 1.5 ± 0.457
1.143GlnLys: 1.143 ± 0.273
2.572GlnLeu: 2.572 ± 0.323
1.358GlnMet: 1.358 ± 0.306
1.0GlnAsn: 1.0 ± 0.318
1.215GlnPro: 1.215 ± 0.332
2.429GlnGln: 2.429 ± 0.602
3.715GlnArg: 3.715 ± 0.649
2.072GlnSer: 2.072 ± 0.421
1.429GlnThr: 1.429 ± 0.372
2.358GlnVal: 2.358 ± 0.451
0.786GlnTrp: 0.786 ± 0.258
1.0GlnTyr: 1.0 ± 0.259
0.0GlnXaa: 0.0 ± 0.0
Arg
5.787ArgAla: 5.787 ± 0.559
0.857ArgCys: 0.857 ± 0.241
4.144ArgAsp: 4.144 ± 0.588
2.787ArgGlu: 2.787 ± 0.404
2.572ArgPhe: 2.572 ± 0.489
3.572ArgGly: 3.572 ± 0.587
1.143ArgHis: 1.143 ± 0.346
3.572ArgIle: 3.572 ± 0.477
2.858ArgLys: 2.858 ± 0.468
4.644ArgLeu: 4.644 ± 0.525
1.5ArgMet: 1.5 ± 0.265
2.572ArgAsn: 2.572 ± 0.328
2.143ArgPro: 2.143 ± 0.386
2.072ArgGln: 2.072 ± 0.479
3.215ArgArg: 3.215 ± 0.703
2.929ArgSer: 2.929 ± 0.511
3.001ArgThr: 3.001 ± 0.528
2.858ArgVal: 2.858 ± 0.527
1.072ArgTrp: 1.072 ± 0.26
1.715ArgTyr: 1.715 ± 0.462
0.0ArgXaa: 0.0 ± 0.0
Ser
5.502SerAla: 5.502 ± 0.56
0.929SerCys: 0.929 ± 0.274
3.215SerAsp: 3.215 ± 0.524
2.429SerGlu: 2.429 ± 0.418
2.929SerPhe: 2.929 ± 0.479
4.144SerGly: 4.144 ± 0.766
0.786SerHis: 0.786 ± 0.271
2.429SerIle: 2.429 ± 0.476
3.072SerLys: 3.072 ± 0.49
3.572SerLeu: 3.572 ± 0.514
1.572SerMet: 1.572 ± 0.316
2.429SerAsn: 2.429 ± 0.405
2.429SerPro: 2.429 ± 0.384
1.643SerGln: 1.643 ± 0.369
2.715SerArg: 2.715 ± 0.447
3.43SerSer: 3.43 ± 0.602
2.858SerThr: 2.858 ± 0.476
4.001SerVal: 4.001 ± 0.472
0.929SerTrp: 0.929 ± 0.28
2.429SerTyr: 2.429 ± 0.379
0.0SerXaa: 0.0 ± 0.0
Thr
6.645ThrAla: 6.645 ± 0.902
0.786ThrCys: 0.786 ± 0.257
4.001ThrAsp: 4.001 ± 0.648
3.715ThrGlu: 3.715 ± 0.508
2.929ThrPhe: 2.929 ± 0.455
6.073ThrGly: 6.073 ± 0.842
0.572ThrHis: 0.572 ± 0.221
4.215ThrIle: 4.215 ± 0.569
2.429ThrLys: 2.429 ± 0.407
4.573ThrLeu: 4.573 ± 0.393
1.5ThrMet: 1.5 ± 0.312
2.143ThrAsn: 2.143 ± 0.479
3.287ThrPro: 3.287 ± 0.451
1.643ThrGln: 1.643 ± 0.267
2.358ThrArg: 2.358 ± 0.399
3.287ThrSer: 3.287 ± 0.527
3.572ThrThr: 3.572 ± 0.656
4.644ThrVal: 4.644 ± 0.685
0.572ThrTrp: 0.572 ± 0.183
1.786ThrTyr: 1.786 ± 0.327
0.0ThrXaa: 0.0 ± 0.0
Val
7.645ValAla: 7.645 ± 0.806
0.572ValCys: 0.572 ± 0.231
4.358ValAsp: 4.358 ± 0.526
5.216ValGlu: 5.216 ± 0.595
3.501ValPhe: 3.501 ± 0.466
6.002ValGly: 6.002 ± 0.706
0.572ValHis: 0.572 ± 0.193
3.572ValIle: 3.572 ± 0.386
4.573ValLys: 4.573 ± 0.729
5.644ValLeu: 5.644 ± 0.653
1.5ValMet: 1.5 ± 0.261
3.715ValAsn: 3.715 ± 0.633
2.929ValPro: 2.929 ± 0.453
1.715ValGln: 1.715 ± 0.434
4.073ValArg: 4.073 ± 0.619
4.43ValSer: 4.43 ± 0.799
5.073ValThr: 5.073 ± 0.8
4.358ValVal: 4.358 ± 0.572
1.215ValTrp: 1.215 ± 0.364
1.786ValTyr: 1.786 ± 0.407
0.0ValXaa: 0.0 ± 0.0
Trp
1.286TrpAla: 1.286 ± 0.286
0.214TrpCys: 0.214 ± 0.126
0.786TrpAsp: 0.786 ± 0.255
0.572TrpGlu: 0.572 ± 0.203
0.857TrpPhe: 0.857 ± 0.202
1.072TrpGly: 1.072 ± 0.352
0.214TrpHis: 0.214 ± 0.139
0.357TrpIle: 0.357 ± 0.175
0.429TrpLys: 0.429 ± 0.19
1.715TrpLeu: 1.715 ± 0.357
0.214TrpMet: 0.214 ± 0.116
1.5TrpAsn: 1.5 ± 0.309
0.429TrpPro: 0.429 ± 0.172
1.143TrpGln: 1.143 ± 0.36
0.929TrpArg: 0.929 ± 0.292
1.0TrpSer: 1.0 ± 0.304
1.572TrpThr: 1.572 ± 0.347
0.857TrpVal: 0.857 ± 0.252
0.214TrpTrp: 0.214 ± 0.127
1.072TrpTyr: 1.072 ± 0.327
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.858TyrAla: 2.858 ± 0.517
0.643TyrCys: 0.643 ± 0.215
2.072TyrAsp: 2.072 ± 0.413
2.072TyrGlu: 2.072 ± 0.423
0.786TyrPhe: 0.786 ± 0.237
2.501TyrGly: 2.501 ± 0.36
0.357TyrHis: 0.357 ± 0.191
1.929TyrIle: 1.929 ± 0.316
1.072TyrLys: 1.072 ± 0.278
1.929TyrLeu: 1.929 ± 0.419
0.714TyrMet: 0.714 ± 0.22
0.929TyrAsn: 0.929 ± 0.286
1.5TyrPro: 1.5 ± 0.281
1.143TyrGln: 1.143 ± 0.382
2.072TyrArg: 2.072 ± 0.379
1.429TyrSer: 1.429 ± 0.346
1.215TyrThr: 1.215 ± 0.371
2.715TyrVal: 2.715 ± 0.485
1.143TyrTrp: 1.143 ± 0.236
0.643TyrTyr: 0.643 ± 0.235
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 60 proteins (13997 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski