Symbiobacterium thermophilum (strain T / IAM 14863)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Symbiobacteriaceae; Symbiobacterium; Symbiobacterium thermophilum

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3313 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q67PL3|Q67PL3_SYMTH Uncharacterized protein OS=Symbiobacterium thermophilum (strain T / IAM 14863) OX=292459 GN=STH1395 PE=4 SV=1
MM1 pKa = 7.46FGFAPVWPAAAPLFAAPVINVWWYY25 pKa = 7.99EE26 pKa = 3.85LKK28 pKa = 10.62NVSSGGVVSTGVLDD42 pKa = 5.26LGFLTSNSKK51 pKa = 10.89GNSLTDD57 pKa = 3.14QGGDD61 pKa = 3.12ATYY64 pKa = 10.84SATWYY69 pKa = 10.69GFLSDD74 pKa = 4.49TDD76 pKa = 3.92WVDD79 pKa = 3.26TPYY82 pKa = 11.8ANMAGFQVGGG92 pKa = 3.62

Molecular weight: 9.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q67J80|Q67J80_SYMTH Histidine kinase OS=Symbiobacterium thermophilum (strain T / IAM 14863) OX=292459 GN=STH3289 PE=4 SV=1
MM1 pKa = 7.39FLLKK5 pKa = 9.89RR6 pKa = 11.84TFQPHH11 pKa = 4.28NRR13 pKa = 11.84SRR15 pKa = 11.84KK16 pKa = 7.02RR17 pKa = 11.84THH19 pKa = 6.3GFLSRR24 pKa = 11.84MSTRR28 pKa = 11.84GGRR31 pKa = 11.84RR32 pKa = 11.84VLKK35 pKa = 10.45ARR37 pKa = 11.84RR38 pKa = 11.84LKK40 pKa = 10.07GRR42 pKa = 11.84KK43 pKa = 9.08RR44 pKa = 11.84LTVV47 pKa = 3.11

Molecular weight: 5.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3313

0

3313

1023222

30

2244

308.9

33.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.123 ± 0.067

0.87 ± 0.015

4.866 ± 0.03

6.612 ± 0.044

3.108 ± 0.028

8.999 ± 0.04

1.973 ± 0.017

4.397 ± 0.033

2.491 ± 0.033

10.974 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.258 ± 0.019

2.069 ± 0.022

5.806 ± 0.039

3.411 ± 0.026

8.321 ± 0.046

4.301 ± 0.026

4.967 ± 0.029

8.493 ± 0.04

1.449 ± 0.02

2.512 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski