Amino acid dipepetide frequency for Symbiobacterium thermophilum (strain T / IAM 14863)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.994AlaAla: 17.994 ± 0.232	1.152AlaCys: 1.152 ± 0.041	6.033AlaAsp: 6.033 ± 0.09	8.796AlaGlu: 8.796 ± 0.09	3.565AlaPhe: 3.565 ± 0.07	11.715AlaGly: 11.715 ± 0.111	2.164AlaHis: 2.164 ± 0.054	4.903AlaIle: 4.903 ± 0.076	2.384AlaLys: 2.384 ± 0.06	14.062AlaLeu: 14.062 ± 0.159	2.796AlaMet: 2.796 ± 0.049	1.995AlaAsn: 1.995 ± 0.058	5.414AlaPro: 5.414 ± 0.099	4.002AlaGln: 4.002 ± 0.074	9.853AlaArg: 9.853 ± 0.124	4.303AlaSer: 4.303 ± 0.081	4.951AlaThr: 4.951 ± 0.073	10.777AlaVal: 10.777 ± 0.117	1.589AlaTrp: 1.589 ± 0.037	2.782AlaTyr: 2.782 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	0.899CysAla: 0.899 ± 0.036	0.117CysCys: 0.117 ± 0.014	0.442CysAsp: 0.442 ± 0.023	0.433CysGlu: 0.433 ± 0.023	0.253CysPhe: 0.253 ± 0.016	1.009CysGly: 1.009 ± 0.036	0.254CysHis: 0.254 ± 0.017	0.359CysIle: 0.359 ± 0.019	0.172CysLys: 0.172 ± 0.015	0.873CysLeu: 0.873 ± 0.028	0.179CysMet: 0.179 ± 0.015	0.21CysAsn: 0.21 ± 0.015	0.639CysPro: 0.639 ± 0.032	0.24CysGln: 0.24 ± 0.016	0.823CysArg: 0.823 ± 0.03	0.442CysSer: 0.442 ± 0.021	0.511CysThr: 0.511 ± 0.021	0.534CysVal: 0.534 ± 0.029	0.107CysTrp: 0.107 ± 0.011	0.208CysTyr: 0.208 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	5.032AspAla: 5.032 ± 0.081	0.354AspCys: 0.354 ± 0.019	2.229AspAsp: 2.229 ± 0.06	3.515AspGlu: 3.515 ± 0.058	1.487AspPhe: 1.487 ± 0.04	4.791AspGly: 4.791 ± 0.085	0.953AspHis: 0.953 ± 0.034	1.919AspIle: 1.919 ± 0.05	1.005AspLys: 1.005 ± 0.037	6.149AspLeu: 6.149 ± 0.085	1.009AspMet: 1.009 ± 0.023	0.767AspAsn: 0.767 ± 0.026	4.029AspPro: 4.029 ± 0.081	1.607AspGln: 1.607 ± 0.044	4.518AspArg: 4.518 ± 0.076	1.516AspSer: 1.516 ± 0.029	1.859AspThr: 1.859 ± 0.035	3.859AspVal: 3.859 ± 0.06	0.831AspTrp: 0.831 ± 0.032	1.235AspTyr: 1.235 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	8.837GluAla: 8.837 ± 0.127	0.38GluCys: 0.38 ± 0.018	2.578GluAsp: 2.578 ± 0.045	5.189GluGlu: 5.189 ± 0.081	1.704GluPhe: 1.704 ± 0.043	5.372GluGly: 5.372 ± 0.072	1.211GluHis: 1.211 ± 0.035	3.375GluIle: 3.375 ± 0.063	2.018GluLys: 2.018 ± 0.057	6.633GluLeu: 6.633 ± 0.097	1.695GluMet: 1.695 ± 0.042	1.387GluAsn: 1.387 ± 0.04	3.628GluPro: 3.628 ± 0.078	2.859GluGln: 2.859 ± 0.061	6.007GluArg: 6.007 ± 0.095	2.374GluSer: 2.374 ± 0.047	2.828GluThr: 2.828 ± 0.056	5.744GluVal: 5.744 ± 0.105	0.867GluTrp: 0.867 ± 0.031	1.432GluTyr: 1.432 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	3.446PheAla: 3.446 ± 0.058	0.324PheCys: 0.324 ± 0.019	1.76PheAsp: 1.76 ± 0.047	1.599PheGlu: 1.599 ± 0.046	1.165PhePhe: 1.165 ± 0.038	3.078PheGly: 3.078 ± 0.061	0.691PheHis: 0.691 ± 0.028	1.372PheIle: 1.372 ± 0.035	0.66PheLys: 0.66 ± 0.032	3.388PheLeu: 3.388 ± 0.079	0.62PheMet: 0.62 ± 0.023	0.719PheAsn: 0.719 ± 0.029	1.56PhePro: 1.56 ± 0.041	0.988PheGln: 0.988 ± 0.033	2.476PheArg: 2.476 ± 0.049	1.544PheSer: 1.544 ± 0.038	1.895PheThr: 1.895 ± 0.048	2.427PheVal: 2.427 ± 0.048	0.501PheTrp: 0.501 ± 0.025	0.862PheTyr: 0.862 ± 0.031	0.0PheXaa: 0.0 ± 0.0
Gly	9.283GlyAla: 9.283 ± 0.131	0.99GlyCys: 0.99 ± 0.034	4.223GlyAsp: 4.223 ± 0.075	5.544GlyGlu: 5.544 ± 0.081	3.094GlyPhe: 3.094 ± 0.055	8.099GlyGly: 8.099 ± 0.127	1.783GlyHis: 1.783 ± 0.05	4.154GlyIle: 4.154 ± 0.065	2.634GlyLys: 2.634 ± 0.073	9.883GlyLeu: 9.883 ± 0.114	2.255GlyMet: 2.255 ± 0.046	1.774GlyAsn: 1.774 ± 0.051	4.668GlyPro: 4.668 ± 0.093	3.129GlyGln: 3.129 ± 0.071	7.841GlyArg: 7.841 ± 0.111	4.406GlySer: 4.406 ± 0.077	4.879GlyThr: 4.879 ± 0.083	7.114GlyVal: 7.114 ± 0.09	1.642GlyTrp: 1.642 ± 0.043	2.596GlyTyr: 2.596 ± 0.053	0.0GlyXaa: 0.0 ± 0.0
His	1.83HisAla: 1.83 ± 0.042	0.22HisCys: 0.22 ± 0.012	0.902HisAsp: 0.902 ± 0.028	1.068HisGlu: 1.068 ± 0.031	0.697HisPhe: 0.697 ± 0.026	1.923HisGly: 1.923 ± 0.047	0.542HisHis: 0.542 ± 0.027	0.781HisIle: 0.781 ± 0.038	0.426HisLys: 0.426 ± 0.023	2.497HisLeu: 2.497 ± 0.058	0.442HisMet: 0.442 ± 0.023	0.413HisAsn: 0.413 ± 0.019	1.589HisPro: 1.589 ± 0.05	0.622HisGln: 0.622 ± 0.026	1.748HisArg: 1.748 ± 0.037	0.754HisSer: 0.754 ± 0.028	0.9HisThr: 0.9 ± 0.03	1.529HisVal: 1.529 ± 0.038	0.33HisTrp: 0.33 ± 0.014	0.515HisTyr: 0.515 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	5.453IleAla: 5.453 ± 0.078	0.453IleCys: 0.453 ± 0.022	2.455IleAsp: 2.455 ± 0.053	2.476IleGlu: 2.476 ± 0.052	1.302IlePhe: 1.302 ± 0.034	3.873IleGly: 3.873 ± 0.062	0.896IleHis: 0.896 ± 0.023	1.869IleIle: 1.869 ± 0.05	1.058IleLys: 1.058 ± 0.041	4.586IleLeu: 4.586 ± 0.081	0.889IleMet: 0.889 ± 0.034	1.09IleAsn: 1.09 ± 0.038	2.565IlePro: 2.565 ± 0.058	1.5IleGln: 1.5 ± 0.043	3.743IleArg: 3.743 ± 0.062	1.965IleSer: 1.965 ± 0.048	2.56IleThr: 2.56 ± 0.058	3.401IleVal: 3.401 ± 0.069	0.55IleTrp: 0.55 ± 0.024	1.28IleTyr: 1.28 ± 0.038	0.0IleXaa: 0.0 ± 0.0
Lys	3.154LysAla: 3.154 ± 0.072	0.176LysCys: 0.176 ± 0.014	1.165LysAsp: 1.165 ± 0.045	1.773LysGlu: 1.773 ± 0.052	0.626LysPhe: 0.626 ± 0.023	2.264LysGly: 2.264 ± 0.06	0.471LysHis: 0.471 ± 0.022	1.154LysIle: 1.154 ± 0.039	0.795LysLys: 0.795 ± 0.037	2.368LysLeu: 2.368 ± 0.056	0.587LysMet: 0.587 ± 0.024	0.557LysAsn: 0.557 ± 0.028	1.436LysPro: 1.436 ± 0.041	0.899LysGln: 0.899 ± 0.034	1.897LysArg: 1.897 ± 0.058	1.15LysSer: 1.15 ± 0.043	1.243LysThr: 1.243 ± 0.046	2.242LysVal: 2.242 ± 0.066	0.265LysTrp: 0.265 ± 0.014	0.685LysTyr: 0.685 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	14.41LeuAla: 14.41 ± 0.174	0.884LeuCys: 0.884 ± 0.034	5.275LeuAsp: 5.275 ± 0.072	6.886LeuGlu: 6.886 ± 0.08	3.463LeuPhe: 3.463 ± 0.059	9.187LeuGly: 9.187 ± 0.104	2.029LeuHis: 2.029 ± 0.047	4.626LeuIle: 4.626 ± 0.074	2.898LeuLys: 2.898 ± 0.053	12.005LeuLeu: 12.005 ± 0.154	2.338LeuMet: 2.338 ± 0.046	2.372LeuAsn: 2.372 ± 0.046	6.167LeuPro: 6.167 ± 0.102	3.668LeuGln: 3.668 ± 0.066	8.753LeuArg: 8.753 ± 0.124	5.574LeuSer: 5.574 ± 0.078	6.168LeuThr: 6.168 ± 0.068	9.204LeuVal: 9.204 ± 0.103	1.387LeuTrp: 1.387 ± 0.042	2.449LeuTyr: 2.449 ± 0.062	0.0LeuXaa: 0.0 ± 0.0
Met	3.139MetAla: 3.139 ± 0.055	0.13MetCys: 0.13 ± 0.015	1.099MetAsp: 1.099 ± 0.031	1.505MetGlu: 1.505 ± 0.039	0.496MetPhe: 0.496 ± 0.023	2.044MetGly: 2.044 ± 0.041	0.373MetHis: 0.373 ± 0.019	0.999MetIle: 0.999 ± 0.036	0.788MetLys: 0.788 ± 0.03	2.254MetLeu: 2.254 ± 0.06	0.526MetMet: 0.526 ± 0.029	0.574MetAsn: 0.574 ± 0.026	1.189MetPro: 1.189 ± 0.037	0.79MetGln: 0.79 ± 0.024	1.863MetArg: 1.863 ± 0.046	1.08MetSer: 1.08 ± 0.034	1.291MetThr: 1.291 ± 0.038	1.82MetVal: 1.82 ± 0.043	0.21MetTrp: 0.21 ± 0.013	0.409MetTyr: 0.409 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	2.107AsnAla: 2.107 ± 0.052	0.238AsnCys: 0.238 ± 0.019	0.816AsnAsp: 0.816 ± 0.024	0.951AsnGlu: 0.951 ± 0.035	0.706AsnPhe: 0.706 ± 0.026	1.708AsnGly: 1.708 ± 0.045	0.41AsnHis: 0.41 ± 0.022	0.997AsnIle: 0.997 ± 0.034	0.487AsnLys: 0.487 ± 0.026	2.658AsnLeu: 2.658 ± 0.052	0.452AsnMet: 0.452 ± 0.021	0.491AsnAsn: 0.491 ± 0.024	1.92AsnPro: 1.92 ± 0.052	0.711AsnGln: 0.711 ± 0.025	1.883AsnArg: 1.883 ± 0.036	0.735AsnSer: 0.735 ± 0.027	0.954AsnThr: 0.954 ± 0.03	1.579AsnVal: 1.579 ± 0.038	0.324AsnTrp: 0.324 ± 0.022	0.564AsnTyr: 0.564 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	7.163ProAla: 7.163 ± 0.128	0.436ProCys: 0.436 ± 0.025	3.928ProAsp: 3.928 ± 0.074	5.074ProGlu: 5.074 ± 0.085	1.778ProPhe: 1.778 ± 0.054	6.475ProGly: 6.475 ± 0.116	1.199ProHis: 1.199 ± 0.043	1.985ProIle: 1.985 ± 0.051	1.271ProLys: 1.271 ± 0.038	5.359ProLeu: 5.359 ± 0.087	1.071ProMet: 1.071 ± 0.031	1.124ProAsn: 1.124 ± 0.031	3.405ProPro: 3.405 ± 0.067	1.656ProGln: 1.656 ± 0.042	3.452ProArg: 3.452 ± 0.06	2.438ProSer: 2.438 ± 0.056	2.513ProThr: 2.513 ± 0.048	5.413ProVal: 5.413 ± 0.066	0.893ProTrp: 0.893 ± 0.037	1.426ProTyr: 1.426 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	4.586GlnAla: 4.586 ± 0.064	0.198GlnCys: 0.198 ± 0.013	1.305GlnAsp: 1.305 ± 0.032	2.385GlnGlu: 2.385 ± 0.065	0.943GlnPhe: 0.943 ± 0.032	2.644GlnGly: 2.644 ± 0.056	0.679GlnHis: 0.679 ± 0.032	1.669GlnIle: 1.669 ± 0.042	1.006GlnLys: 1.006 ± 0.042	3.21GlnLeu: 3.21 ± 0.064	0.892GlnMet: 0.892 ± 0.032	0.752GlnAsn: 0.752 ± 0.025	1.98GlnPro: 1.98 ± 0.046	1.489GlnGln: 1.489 ± 0.043	2.79GlnArg: 2.79 ± 0.059	1.262GlnSer: 1.262 ± 0.034	1.48GlnThr: 1.48 ± 0.039	3.536GlnVal: 3.536 ± 0.067	0.471GlnTrp: 0.471 ± 0.021	0.837GlnTyr: 0.837 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	8.76ArgAla: 8.76 ± 0.12	0.725ArgCys: 0.725 ± 0.028	3.398ArgAsp: 3.398 ± 0.064	5.989ArgGlu: 5.989 ± 0.095	2.925ArgPhe: 2.925 ± 0.053	5.655ArgGly: 5.655 ± 0.074	1.699ArgHis: 1.699 ± 0.041	4.09ArgIle: 4.09 ± 0.075	2.198ArgLys: 2.198 ± 0.049	9.791ArgLeu: 9.791 ± 0.142	2.224ArgMet: 2.224 ± 0.043	1.654ArgAsn: 1.654 ± 0.044	4.852ArgPro: 4.852 ± 0.077	3.101ArgGln: 3.101 ± 0.064	8.004ArgArg: 8.004 ± 0.125	3.763ArgSer: 3.763 ± 0.07	4.029ArgThr: 4.029 ± 0.079	6.571ArgVal: 6.571 ± 0.09	1.468ArgTrp: 1.468 ± 0.044	2.318ArgTyr: 2.318 ± 0.064	0.0ArgXaa: 0.0 ± 0.0
Ser	4.883SerAla: 4.883 ± 0.087	0.391SerCys: 0.391 ± 0.02	2.005SerAsp: 2.005 ± 0.046	2.188SerGlu: 2.188 ± 0.047	1.506SerPhe: 1.506 ± 0.033	4.759SerGly: 4.759 ± 0.065	0.899SerHis: 0.899 ± 0.024	1.732SerIle: 1.732 ± 0.044	0.918SerLys: 0.918 ± 0.031	4.926SerLeu: 4.926 ± 0.081	0.974SerMet: 0.974 ± 0.026	0.835SerAsn: 0.835 ± 0.033	2.899SerPro: 2.899 ± 0.052	1.39SerGln: 1.39 ± 0.046	3.521SerArg: 3.521 ± 0.063	2.027SerSer: 2.027 ± 0.043	2.117SerThr: 2.117 ± 0.05	3.349SerVal: 3.349 ± 0.049	0.624SerTrp: 0.624 ± 0.025	1.07SerTyr: 1.07 ± 0.031	0.0SerXaa: 0.0 ± 0.0
Thr	5.898ThrAla: 5.898 ± 0.084	0.437ThrCys: 0.437 ± 0.022	2.461ThrAsp: 2.461 ± 0.05	2.714ThrGlu: 2.714 ± 0.05	1.762ThrPhe: 1.762 ± 0.043	5.395ThrGly: 5.395 ± 0.085	0.94ThrHis: 0.94 ± 0.028	2.261ThrIle: 2.261 ± 0.053	0.969ThrLys: 0.969 ± 0.038	5.27ThrLeu: 5.27 ± 0.066	1.009ThrMet: 1.009 ± 0.037	1.087ThrAsn: 1.087 ± 0.032	3.27ThrPro: 3.27 ± 0.058	1.301ThrGln: 1.301 ± 0.034	3.381ThrArg: 3.381 ± 0.064	2.026ThrSer: 2.026 ± 0.046	2.433ThrThr: 2.433 ± 0.061	4.976ThrVal: 4.976 ± 0.067	0.733ThrTrp: 0.733 ± 0.029	1.345ThrTyr: 1.345 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	10.273ValAla: 10.273 ± 0.098	0.713ValCys: 0.713 ± 0.021	4.57ValAsp: 4.57 ± 0.066	5.723ValGlu: 5.723 ± 0.087	2.261ValPhe: 2.261 ± 0.046	6.558ValGly: 6.558 ± 0.092	1.654ValHis: 1.654 ± 0.057	4.073ValIle: 4.073 ± 0.086	2.273ValLys: 2.273 ± 0.058	9.127ValLeu: 9.127 ± 0.108	1.783ValMet: 1.783 ± 0.043	1.943ValAsn: 1.943 ± 0.046	4.647ValPro: 4.647 ± 0.069	2.677ValGln: 2.677 ± 0.052	7.098ValArg: 7.098 ± 0.099	3.916ValSer: 3.916 ± 0.066	5.009ValThr: 5.009 ± 0.071	7.667ValVal: 7.667 ± 0.114	0.998ValTrp: 0.998 ± 0.031	1.97ValTyr: 1.97 ± 0.044	0.0ValXaa: 0.0 ± 0.0
Trp	1.522TrpAla: 1.522 ± 0.042	0.132TrpCys: 0.132 ± 0.011	0.678TrpAsp: 0.678 ± 0.023	0.831TrpGlu: 0.831 ± 0.03	0.504TrpPhe: 0.504 ± 0.024	1.096TrpGly: 1.096 ± 0.034	0.283TrpHis: 0.283 ± 0.017	0.603TrpIle: 0.603 ± 0.027	0.369TrpLys: 0.369 ± 0.019	1.816TrpLeu: 1.816 ± 0.04	0.358TrpMet: 0.358 ± 0.019	0.36TrpAsn: 0.36 ± 0.018	0.807TrpPro: 0.807 ± 0.024	0.574TrpGln: 0.574 ± 0.022	1.214TrpArg: 1.214 ± 0.038	0.706TrpSer: 0.706 ± 0.033	0.751TrpThr: 0.751 ± 0.029	1.238TrpVal: 1.238 ± 0.045	0.278TrpTrp: 0.278 ± 0.016	0.374TrpTyr: 0.374 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.562TyrAla: 2.562 ± 0.048	0.25TyrCys: 0.25 ± 0.016	1.342TyrAsp: 1.342 ± 0.04	1.48TyrGlu: 1.48 ± 0.034	0.838TyrPhe: 0.838 ± 0.027	2.347TyrGly: 2.347 ± 0.053	0.598TyrHis: 0.598 ± 0.026	1.047TyrIle: 1.047 ± 0.033	0.58TyrLys: 0.58 ± 0.021	2.887TyrLeu: 2.887 ± 0.054	0.48TyrMet: 0.48 ± 0.022	0.59TyrAsn: 0.59 ± 0.026	1.393TyrPro: 1.393 ± 0.039	0.912TyrGln: 0.912 ± 0.036	2.347TyrArg: 2.347 ± 0.055	1.033TyrSer: 1.033 ± 0.036	1.297TyrThr: 1.297 ± 0.031	1.952TyrVal: 1.952 ± 0.051	0.424TyrTrp: 0.424 ± 0.017	0.762TyrTyr: 0.762 ± 0.033	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3313 proteins (1023222 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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