Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Xanthobacteraceae; Xanthobacter; Xanthobacter autotrophicus

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4971 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A7IDR0|A7IDR0_XANP2 Phenylacetic acid degradation protein PaaD OS=Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) OX=78245 GN=Xaut_0902 PE=4 SV=1
MM1 pKa = 7.28ATRR4 pKa = 11.84TITWAWGAQEE14 pKa = 4.68VVSFDD19 pKa = 3.63PATDD23 pKa = 3.38VLDD26 pKa = 4.49FGWLNASDD34 pKa = 3.82FTITEE39 pKa = 4.2VNGSVVIALPANAHH53 pKa = 6.18SYY55 pKa = 8.3TLEE58 pKa = 4.24GVSLSDD64 pKa = 4.49LSLANISAFDD74 pKa = 3.75ASVFAQWSAAIAADD88 pKa = 3.94GATTPVAGTLFALSAARR105 pKa = 11.84DD106 pKa = 3.66TDD108 pKa = 3.89TVLSFNPAADD118 pKa = 3.68KK119 pKa = 11.34LDD121 pKa = 3.74FSGLSASDD129 pKa = 3.47FSIAEE134 pKa = 4.11VNGSVVIYY142 pKa = 9.8LSAEE146 pKa = 3.78RR147 pKa = 11.84QTYY150 pKa = 9.99ILDD153 pKa = 3.92GVTLSQLSLSSIASQDD169 pKa = 3.46AAVLAEE175 pKa = 4.55WSQALASPAGSSAAPTSGGGEE196 pKa = 3.9AVTTTLGWNWNTQTVLAFDD215 pKa = 4.25PARR218 pKa = 11.84DD219 pKa = 3.82KK220 pKa = 11.22IDD222 pKa = 4.18FGWLTPQDD230 pKa = 3.84FSVVEE235 pKa = 4.11LNGSVVILMPGAQQSYY251 pKa = 7.31TLEE254 pKa = 4.28GVTLAEE260 pKa = 4.38LSAANITARR269 pKa = 11.84SAVVLEE275 pKa = 4.57EE276 pKa = 3.85WATLLSGASGSGGGSGGGSGSTLPVIEE303 pKa = 4.1ASAWIDD309 pKa = 3.09AKK311 pKa = 11.18AYY313 pKa = 9.84VAGDD317 pKa = 3.88LVSVGHH323 pKa = 7.11LVYY326 pKa = 10.31QANWWTLGTDD336 pKa = 3.37PTSDD340 pKa = 3.12HH341 pKa = 6.31GAVGTGHH348 pKa = 5.59VWSVVGYY355 pKa = 11.29ADD357 pKa = 4.37LTPVAPDD364 pKa = 3.75APDD367 pKa = 3.88GLHH370 pKa = 7.13LLTTTEE376 pKa = 4.17TSATLTWDD384 pKa = 3.17AAEE387 pKa = 4.07VSGVGTVTGYY397 pKa = 11.16AIYY400 pKa = 10.22RR401 pKa = 11.84DD402 pKa = 3.98GEE404 pKa = 4.49LVGTTTDD411 pKa = 3.3LSFKK415 pKa = 10.76VSGLVADD422 pKa = 4.25TTYY425 pKa = 10.56HH426 pKa = 6.1FSVVAVDD433 pKa = 3.75EE434 pKa = 5.22AGTSPAATPIAVTTDD449 pKa = 3.07APGTDD454 pKa = 4.02AADD457 pKa = 3.53QQHH460 pKa = 6.18FSPYY464 pKa = 10.04FEE466 pKa = 4.05MWLPSSQNLVQTVEE480 pKa = 4.4DD481 pKa = 3.67VGLTAVTLAFVLGTGPDD498 pKa = 3.45QIGWGGLGSIDD509 pKa = 5.26NDD511 pKa = 3.64TLANGTTISSMVAALQQNGVEE532 pKa = 4.33VTISFGGGYY541 pKa = 8.99GQEE544 pKa = 4.11PALSFTNVAQLTAAYY559 pKa = 9.58QSVMDD564 pKa = 4.61KK565 pKa = 11.47YY566 pKa = 11.44NVTSLDD572 pKa = 3.38FDD574 pKa = 3.92IEE576 pKa = 4.41ADD578 pKa = 3.48ALTNTAASHH587 pKa = 6.1LRR589 pKa = 11.84NEE591 pKa = 4.29ALVALEE597 pKa = 4.1QANPDD602 pKa = 3.51LTVSFTLPALPTGLNQAGLDD622 pKa = 3.72LLAQAKK628 pKa = 10.26ADD630 pKa = 3.49GVEE633 pKa = 3.87IDD635 pKa = 3.95TVNIMVMNYY644 pKa = 9.07GAYY647 pKa = 9.93YY648 pKa = 10.69DD649 pKa = 4.12SGDD652 pKa = 3.68MGKK655 pKa = 10.07DD656 pKa = 3.15AIDD659 pKa = 3.58AAEE662 pKa = 3.8ATIAQLHH669 pKa = 5.07QLGLDD674 pKa = 3.35AKK676 pKa = 10.46VAITPMIGQNDD687 pKa = 3.57VPGEE691 pKa = 4.22VFTLDD696 pKa = 4.66DD697 pKa = 3.85ARR699 pKa = 11.84QLLDD703 pKa = 3.41YY704 pKa = 11.56AEE706 pKa = 4.92GNDD709 pKa = 4.05HH710 pKa = 6.81IAYY713 pKa = 9.35IAMWSLGRR721 pKa = 11.84DD722 pKa = 3.07HH723 pKa = 7.29GDD725 pKa = 3.38DD726 pKa = 3.55VGHH729 pKa = 6.37LTEE732 pKa = 4.57TSSGVAQHH740 pKa = 7.29DD741 pKa = 3.83YY742 pKa = 11.47DD743 pKa = 3.73FAKK746 pKa = 10.12IFSMVV751 pKa = 2.75

Molecular weight: 77.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|A7IGN8|IF3_XANP2 Translation initiation factor IF-3 OS=Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) OX=78245 GN=infC PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 9.26IIAARR34 pKa = 11.84RR35 pKa = 11.84NHH37 pKa = 4.53GRR39 pKa = 11.84QRR41 pKa = 11.84LSAA44 pKa = 4.08

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4971

0

4971

1618488

35

3208

325.6

35.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.793 ± 0.059

0.803 ± 0.009

5.556 ± 0.028

5.548 ± 0.036

3.671 ± 0.022

8.761 ± 0.037

1.995 ± 0.016

4.583 ± 0.027

3.069 ± 0.029

10.319 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.264 ± 0.015

2.201 ± 0.019

5.688 ± 0.033

2.881 ± 0.021

7.427 ± 0.04

5.055 ± 0.021

5.226 ± 0.026

7.847 ± 0.026

1.246 ± 0.012

2.069 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski