Propionimicrobium lymphophilum ACS-093-V-SCH5
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2076 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S2VYT4|S2VYT4_9ACTN Uncharacterized protein OS=Propionimicrobium lymphophilum ACS-093-V-SCH5 OX=883161 GN=HMPREF9306_01377 PE=4 SV=1
MM1 pKa = 6.39 ITNYY5 pKa = 10.07 KK6 pKa = 7.62 ITGMTCGHH14 pKa = 6.76 CVNAVTSEE22 pKa = 3.81 VSEE25 pKa = 4.1 IDD27 pKa = 3.26 GVTDD31 pKa = 3.4 VDD33 pKa = 3.93 VQLEE37 pKa = 4.27 GGKK40 pKa = 8.58 MAVTSEE46 pKa = 4.2 SEE48 pKa = 3.72 IDD50 pKa = 3.56 FEE52 pKa = 5.09 AIKK55 pKa = 10.42 EE56 pKa = 4.27 AVDD59 pKa = 3.27 EE60 pKa = 4.83 AGDD63 pKa = 3.89 YY64 pKa = 9.83 TVEE67 pKa = 4.07 LAA69 pKa = 4.92
Molecular weight: 7.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.779
IPC2_protein 3.846
IPC_protein 3.732
Toseland 3.567
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.63
Rodwell 3.579
Grimsley 3.478
Solomon 3.681
Lehninger 3.643
Nozaki 3.846
DTASelect 3.973
Thurlkill 3.617
EMBOSS 3.643
Sillero 3.859
Patrickios 1.85
IPC_peptide 3.694
IPC2_peptide 3.834
IPC2.peptide.svr19 3.769
Protein with the highest isoelectric point:
>tr|S2W4D3|S2W4D3_9ACTN DNA gyrase subunit B OS=Propionimicrobium lymphophilum ACS-093-V-SCH5 OX=883161 GN=gyrB PE=3 SV=1
MM1 pKa = 7.05 NALVAGLGAFLFLFGAFVVVVGARR25 pKa = 11.84 SSSSRR30 pKa = 11.84 IVSKK34 pKa = 10.33 PLNLPKK40 pKa = 10.26 ISVRR44 pKa = 11.84 TGGVLALSALFALVSGWLPALIVGPGVGLVLPDD77 pKa = 4.06 LLRR80 pKa = 11.84 PKK82 pKa = 10.23 PNRR85 pKa = 11.84 EE86 pKa = 4.02 VQLLAALDD94 pKa = 3.37 RR95 pKa = 11.84 WVRR98 pKa = 11.84 LLTASVATGKK108 pKa = 10.46 SIPDD112 pKa = 3.45 AMRR115 pKa = 11.84 TTYY118 pKa = 10.32 AQCPKK123 pKa = 8.93 MLKK126 pKa = 10.16 SGVSTALARR135 pKa = 11.84 LRR137 pKa = 11.84 GRR139 pKa = 11.84 WSVRR143 pKa = 11.84 EE144 pKa = 3.97 SLLALADD151 pKa = 3.68 EE152 pKa = 4.79 WDD154 pKa = 3.86 STDD157 pKa = 3.59 ADD159 pKa = 3.79 AVLAACILCSEE170 pKa = 4.3 RR171 pKa = 11.84 GGLGAHH177 pKa = 6.14 RR178 pKa = 11.84 TLDD181 pKa = 3.56 ALADD185 pKa = 3.99 SVSDD189 pKa = 3.29 RR190 pKa = 11.84 LAALRR195 pKa = 11.84 EE196 pKa = 3.87 IDD198 pKa = 4.07 AEE200 pKa = 4.12 RR201 pKa = 11.84 AKK203 pKa = 10.66 PRR205 pKa = 11.84 VVVRR209 pKa = 11.84 QVTMITVVVLGAGVVINPSYY229 pKa = 10.27 FAPFTTPIGQVLAIGFFVCYY249 pKa = 9.29 MLSLRR254 pKa = 11.84 MLQRR258 pKa = 11.84 MSRR261 pKa = 11.84 PQKK264 pKa = 10.14 RR265 pKa = 11.84 EE266 pKa = 3.48 RR267 pKa = 11.84 ILQVNQQ273 pKa = 3.01
Molecular weight: 29.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.516
IPC_protein 10.335
Toseland 10.701
ProMoST 10.423
Dawson 10.774
Bjellqvist 10.511
Wikipedia 10.994
Rodwell 10.891
Grimsley 10.804
Solomon 10.921
Lehninger 10.891
Nozaki 10.701
DTASelect 10.496
Thurlkill 10.687
EMBOSS 11.111
Sillero 10.716
Patrickios 10.628
IPC_peptide 10.935
IPC2_peptide 9.692
IPC2.peptide.svr19 8.676
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2076
0
2076
682374
31
2653
328.7
35.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.571 ± 0.067
0.942 ± 0.015
6.086 ± 0.041
6.39 ± 0.045
3.397 ± 0.037
8.034 ± 0.049
1.773 ± 0.022
5.437 ± 0.042
4.699 ± 0.044
9.552 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.315 ± 0.026
3.382 ± 0.029
4.735 ± 0.03
3.466 ± 0.03
5.726 ± 0.053
6.368 ± 0.042
5.455 ± 0.042
7.886 ± 0.05
1.417 ± 0.019
2.369 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here