Ornatilinea apprima
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3344 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0P6XD38|A0A0P6XD38_9CHLR Glycerol-3-phosphate acyltransferase OS=Ornatilinea apprima OX=1134406 GN=plsY PE=3 SV=1
MM1 pKa = 7.22 HH2 pKa = 7.73 RR3 pKa = 11.84 SFHH6 pKa = 6.56 CLLTACLLIGLFSPFAAAPVLAQTPSADD34 pKa = 2.92 VSVAVEE40 pKa = 4.69 FSPSTIALKK49 pKa = 10.59 SVSRR53 pKa = 11.84 LTFTVTNANATHH65 pKa = 7.47 DD66 pKa = 3.95 LTGLAFSNTFPAGLSLADD84 pKa = 4.24 PVNLAQTGCGGASITANPGEE104 pKa = 4.32 SSISLSSGVVASGGSCAVTVSVQPSTSGNKK134 pKa = 8.33 TVTVAAEE141 pKa = 4.15 DD142 pKa = 3.5 VTGYY146 pKa = 10.45 IDD148 pKa = 4.01 GSNVSNEE155 pKa = 3.64 AAASDD160 pKa = 4.18 EE161 pKa = 4.34 LSVATFDD168 pKa = 4.35 YY169 pKa = 10.77 NAEE172 pKa = 3.99 INKK175 pKa = 8.83 QFNPISISPGDD186 pKa = 3.56 ISRR189 pKa = 11.84 LSVSIYY195 pKa = 9.92 NANSFDD201 pKa = 3.94 LTTASWTDD209 pKa = 3.26 NLEE212 pKa = 4.08 GVQPGLYY219 pKa = 9.35 IANPPNVSTSNCGTAPEE236 pKa = 4.23 VTAVAGSSTLTLANATVPAKK256 pKa = 10.46 VGEE259 pKa = 4.25 INGVCTVEE267 pKa = 4.11 VDD269 pKa = 3.64 VSSITPGNLINTIAGGNLSATGNLGTVSNTDD300 pKa = 3.54 PASATINVDD309 pKa = 4.03 TIQAPSVTKK318 pKa = 10.66 AFAPNTIWVGQTSRR332 pKa = 11.84 LTITIRR338 pKa = 11.84 NNDD341 pKa = 3.16 SSHH344 pKa = 6.26 NLTEE348 pKa = 3.94 VSLADD353 pKa = 3.6 VLNDD357 pKa = 3.9 PEE359 pKa = 4.12 PHH361 pKa = 5.75 NVVLASTVSPSLSNCGGSAVLTAVAGTDD389 pKa = 3.22 NLSIANAQISPNTTCSIAVNVTSFTQGEE417 pKa = 4.51 YY418 pKa = 10.69 TNTIPASAVQTRR430 pKa = 11.84 QGVTNATPAQANLNVQAVGLVKK452 pKa = 10.54 SFSPTTILAGEE463 pKa = 4.46 ISTLTITLRR472 pKa = 11.84 NPTGTPYY479 pKa = 10.51 TGVALDD485 pKa = 4.3 DD486 pKa = 4.17 VMPGAALYY494 pKa = 8.33 VTGTPTTTCVGGSVSIATTSRR515 pKa = 11.84 TDD517 pKa = 3.09 DD518 pKa = 3.55 TVRR521 pKa = 11.84 LTGATVPSGTVSSPGTCTITAQITTPDD548 pKa = 3.77 TILTSSHH555 pKa = 6.45 LNEE558 pKa = 4.52 IPAGSLSSDD567 pKa = 3.31 VPGFTNVLPASATLNVQPLSIGVVKK592 pKa = 9.81 TFSPTSFEE600 pKa = 4.05 QGGQSTLIVTLQNSTSSPLHH620 pKa = 4.54 VTQLVDD626 pKa = 3.6 NLPSGLVPDD635 pKa = 4.92 PATVATTCANGQAVAVVGSPSTLTLSGLDD664 pKa = 3.37 ASGAAIPSGTVSVPGTCTFSVKK686 pKa = 8.04 VTAPANASYY695 pKa = 11.11 TNTINANSITTVEE708 pKa = 4.12 GLTNLSSASRR718 pKa = 11.84 SVTVYY723 pKa = 8.38 PTGQGMTGSSKK734 pKa = 9.62 TFSPDD739 pKa = 2.87 IISAGGNSRR748 pKa = 11.84 LRR750 pKa = 11.84 IYY752 pKa = 9.61 LTAPADD758 pKa = 3.67 EE759 pKa = 5.9 AITGLTVTDD768 pKa = 3.8 VLPTNITISNSTAASNSCGGTLTANTGEE796 pKa = 4.28 GTITLTGGSIAMGGNCTIDD815 pKa = 3.44 VYY817 pKa = 10.37 VTSSVSGLYY826 pKa = 10.86 SNTINAATQISNDD839 pKa = 3.25 QGQRR843 pKa = 11.84 PPNNLIDD850 pKa = 5.05 DD851 pKa = 4.3 LTVSNMTISKK861 pKa = 10.32 AFYY864 pKa = 8.87 PSQVAPGGRR873 pKa = 11.84 STLTITLRR881 pKa = 11.84 NTNTAPLINVAITQDD896 pKa = 3.44 NLNTMGGTSFTVANPSNKK914 pKa = 8.82 STTCGGTVTADD925 pKa = 3.09 SGSQIISLSGGSVPAQVGGIAGICTVSVDD954 pKa = 4.01 VVSSPSTTPGDD965 pKa = 3.7 KK966 pKa = 10.79 TNTLYY971 pKa = 9.44 RR972 pKa = 11.84 TNVSGTIQGAGTVIRR987 pKa = 11.84 PVANATAQLTVSSLTLGVVKK1007 pKa = 10.57 GFDD1010 pKa = 3.64 PLTVFGGSSSTMSVQLINPNNTPLTGIAFTDD1041 pKa = 3.61 SMPTGMYY1048 pKa = 9.06 VANPADD1054 pKa = 3.89 VSTGTCGGTLTAVPGAGSFSFSGGSLAANKK1084 pKa = 10.19 RR1085 pKa = 11.84 CTLTLSVTMNVNGNRR1100 pKa = 11.84 TNTIPAEE1107 pKa = 4.24 GVTSFNGAKK1116 pKa = 10.16 NSQPAQASLTNLPGVSISKK1135 pKa = 10.03 YY1136 pKa = 9.43 FAPTSMILGEE1146 pKa = 4.26 TSLLTIQIKK1155 pKa = 8.38 NTGNIALSNLGMVDD1169 pKa = 3.68 TLPAGLTIASSPSPTNACNGLLTAAPGTNTISLGAGSIAPGPNTTCNITVPVTASAAGTYY1229 pKa = 10.91 NNTIGKK1235 pKa = 8.07 NTVTTAEE1242 pKa = 4.48 GATNTEE1248 pKa = 4.29 PATDD1252 pKa = 3.61 TLTVNAAPTMQMVKK1266 pKa = 9.21 TFDD1269 pKa = 3.4 VAGSSEE1275 pKa = 4.0 APYY1278 pKa = 10.66 TDD1280 pKa = 4.18 GDD1282 pKa = 3.95 TLAYY1286 pKa = 9.42 TLTATNTGDD1295 pKa = 3.39 VPLTGVEE1302 pKa = 3.8 ISDD1305 pKa = 3.79 PGVVLGTCTPPQPASLAPGEE1325 pKa = 4.78 SLTCPATHH1333 pKa = 6.91 IVTADD1338 pKa = 3.23 EE1339 pKa = 4.41 AAAGSYY1345 pKa = 10.35 TNTASADD1352 pKa = 3.49 SDD1354 pKa = 3.86 QIGPVTDD1361 pKa = 3.62 SVTVPTDD1368 pKa = 2.97 QTNILGISKK1377 pKa = 10.52 VITTAGPYY1385 pKa = 10.18 EE1386 pKa = 4.62 LGDD1389 pKa = 3.61 TLNYY1393 pKa = 9.14 TITVTNIGSGTLNNVGVADD1412 pKa = 4.03 PGMVLGACTPPQPATLTSGQSMSCPASHH1440 pKa = 6.82 VVTQADD1446 pKa = 3.21 VDD1448 pKa = 3.84 AGTFTNTATGDD1459 pKa = 3.51 SSEE1462 pKa = 4.34 TDD1464 pKa = 3.3 PVTDD1468 pKa = 3.45 SVTVEE1473 pKa = 3.78 IKK1475 pKa = 10.83 DD1476 pKa = 3.57 NARR1479 pKa = 11.84 LEE1481 pKa = 4.4 VYY1483 pKa = 10.27 KK1484 pKa = 10.54 QVTSNGPYY1492 pKa = 8.11 NTVGKK1497 pKa = 8.64 TITYY1501 pKa = 9.41 DD1502 pKa = 3.1 ISAINTGDD1510 pKa = 3.32 QTLTGVTITDD1520 pKa = 3.89 PGTGVTLGACTPAQPATLTTGQILSCAASHH1550 pKa = 7.35 DD1551 pKa = 3.84 VTADD1555 pKa = 4.09 DD1556 pKa = 3.45 ITAGGFSNTAYY1567 pKa = 10.44 ADD1569 pKa = 3.62 SDD1571 pKa = 3.53 QTSPVSSTAEE1581 pKa = 4.21 VITQTPKK1588 pKa = 10.19 IDD1590 pKa = 3.57 LAKK1593 pKa = 10.27 TGTLNLGANGRR1604 pKa = 11.84 ADD1606 pKa = 3.77 AGDD1609 pKa = 4.0 TITYY1613 pKa = 10.22 AFTVTNTGEE1622 pKa = 4.07 VTLNNITLIDD1632 pKa = 3.52 IVGGVSISGGPIASLAPLASDD1653 pKa = 3.09 STTFTGSYY1661 pKa = 9.36 TLTQADD1667 pKa = 3.77 VNAGNFTNTASVTGAPPVGNIVSDD1691 pKa = 3.93 TDD1693 pKa = 3.62 NDD1695 pKa = 4.29 TQTLTPAASIDD1706 pKa = 3.81 LQKK1709 pKa = 10.1 TGTLNLNASAPNGIANPGDD1728 pKa = 3.79 TISYY1732 pKa = 10.34 AFTVTNTGNVTLTNITLADD1751 pKa = 3.77 TVGGISISGGPIASLAPGASNSSTFTGSYY1780 pKa = 9.33 TLNQTNVDD1788 pKa = 3.61 AGTFTNTATVTGTPPSGPNVTDD1810 pKa = 4.87 ADD1812 pKa = 5.11 DD1813 pKa = 4.06 DD1814 pKa = 4.15 TQTIIPVPSIEE1825 pKa = 4.08 LVKK1828 pKa = 10.33 TGTLNLGANSRR1839 pKa = 11.84 ADD1841 pKa = 3.43 AGDD1844 pKa = 3.84 TITYY1848 pKa = 10.22 AFTVTNTGNVTLSGVTLSDD1867 pKa = 3.49 PKK1869 pKa = 9.79 ITEE1872 pKa = 3.82 SGGPITLAPGASNSTAFTGTYY1893 pKa = 9.49 TLTQADD1899 pKa = 4.07 VNAGTFTNTATVTGTTPGGPTVNDD1923 pKa = 3.56 SDD1925 pKa = 5.88 GDD1927 pKa = 4.14 TQTLDD1932 pKa = 3.7 PAPSVALQKK1941 pKa = 9.88 TGTLNDD1947 pKa = 4.32 GPDD1950 pKa = 3.69 GEE1952 pKa = 4.57 PDD1954 pKa = 2.98 EE1955 pKa = 5.77 GEE1957 pKa = 3.89 IITYY1961 pKa = 9.4 VFAVTNTGNVTLTNLTVTEE1980 pKa = 4.32 TVGGITLTGSPIASLEE1996 pKa = 4.06 PGASNNTNYY2005 pKa = 9.63 TGSYY2009 pKa = 9.37 TLTQEE2014 pKa = 4.63 VIDD2017 pKa = 4.84 SGTFTNTAQVSGTPPSGSPVTDD2039 pKa = 3.58 SDD2041 pKa = 6.15 DD2042 pKa = 3.7 DD2043 pKa = 4.14 TQLLTDD2049 pKa = 4.04 VPAITLEE2056 pKa = 4.16 KK2057 pKa = 10.21 TGTLNMDD2064 pKa = 3.68 VVAPDD2069 pKa = 3.64 DD2070 pKa = 3.86 RR2071 pKa = 11.84 ADD2073 pKa = 3.4 VGDD2076 pKa = 4.24 TIRR2079 pKa = 11.84 YY2080 pKa = 8.71 AFTVSNTGNVTLTDD2094 pKa = 3.07 ITLADD2099 pKa = 3.75 TVGGITLAGGPITSLDD2115 pKa = 4.0 PDD2117 pKa = 4.82 DD2118 pKa = 5.61 SDD2120 pKa = 5.07 SDD2122 pKa = 4.14 TFTGVYY2128 pKa = 9.46 TLTQADD2134 pKa = 3.42 IDD2136 pKa = 4.2 AGTFTNTAEE2145 pKa = 4.44 VTATPPEE2152 pKa = 4.65 GDD2154 pKa = 4.42 DD2155 pKa = 3.57 VTGSDD2160 pKa = 5.98 DD2161 pKa = 3.75 DD2162 pKa = 4.24 TQTLAEE2168 pKa = 4.06 TARR2171 pKa = 11.84 IGVAKK2176 pKa = 10.15 RR2177 pKa = 11.84 VTGSPAEE2184 pKa = 4.02 ITPGVWDD2191 pKa = 3.62 VTFEE2195 pKa = 4.0 ILVRR2199 pKa = 11.84 NYY2201 pKa = 11.32 GNVTLSSIQVTDD2213 pKa = 3.85 DD2214 pKa = 3.75 LSAVFPPEE2222 pKa = 3.75 SPFAVQSLTSDD2233 pKa = 3.41 DD2234 pKa = 3.86 FTVNSAYY2241 pKa = 10.36 DD2242 pKa = 3.37 GRR2244 pKa = 11.84 TDD2246 pKa = 4.72 ANLLDD2251 pKa = 4.55 GTDD2254 pKa = 3.26 SLAFGEE2260 pKa = 4.39 QGSITLVVRR2269 pKa = 11.84 VTPADD2274 pKa = 3.6 GGPFNNTAAASAISPSEE2291 pKa = 4.2 SPVSDD2296 pKa = 3.46 TSHH2299 pKa = 6.76 NGANPDD2305 pKa = 3.78 PDD2307 pKa = 4.13 YY2308 pKa = 11.74 DD2309 pKa = 5.5 GDD2311 pKa = 3.93 PTDD2314 pKa = 5.04 NNDD2317 pKa = 3.32 PTPVDD2322 pKa = 4.57 FGADD2326 pKa = 3.52 LFDD2329 pKa = 4.27 PPYY2332 pKa = 10.66 GIKK2335 pKa = 9.72 TYY2337 pKa = 11.02 DD2338 pKa = 3.48 SFGMPLLQWTMVWINNSNIVNVGARR2363 pKa = 11.84 VSDD2366 pKa = 5.18 PIPSGAVYY2374 pKa = 9.34 EE2375 pKa = 4.32 ASGLPSGTGIPDD2387 pKa = 4.25 GAPAGSTDD2395 pKa = 3.18 VGVVCNPLGIIDD2407 pKa = 4.48 PKK2409 pKa = 10.47 EE2410 pKa = 3.65 ISPTISLSATFTTWCYY2426 pKa = 11.25 YY2427 pKa = 10.08 EE2428 pKa = 5.75 GPTLTYY2434 pKa = 9.54 PRR2436 pKa = 11.84 GRR2438 pKa = 11.84 IVWEE2442 pKa = 4.34 GALGPDD2448 pKa = 5.26 LGITDD2453 pKa = 3.9 PALAEE2458 pKa = 4.12 NEE2460 pKa = 3.91 LTITFNLRR2468 pKa = 11.84 VLEE2471 pKa = 4.41 GEE2473 pKa = 4.13 HH2474 pKa = 6.7 EE2475 pKa = 4.3 INNRR2479 pKa = 11.84 ATIDD2483 pKa = 3.1 VDD2485 pKa = 4.02 RR2486 pKa = 11.84 NGNGLIEE2493 pKa = 4.72 GEE2495 pKa = 4.6 TEE2497 pKa = 3.94 VEE2499 pKa = 3.88 AASATEE2505 pKa = 4.2 TWSNWPEE2512 pKa = 3.91 ALPLTGFAPGKK2523 pKa = 9.0 ITDD2526 pKa = 3.7 LSGRR2530 pKa = 11.84 PWVEE2534 pKa = 3.26 YY2535 pKa = 10.54 DD2536 pKa = 3.46 QSQDD2540 pKa = 3.49 LLLEE2544 pKa = 4.32 IPALNVKK2551 pKa = 9.74 VPILGVPIVHH2561 pKa = 6.74 GEE2563 pKa = 3.95 WNLDD2567 pKa = 3.31 WLGGNAGYY2575 pKa = 10.71 LEE2577 pKa = 4.13 NTAFPTHH2584 pKa = 6.87 AGNTGITAHH2593 pKa = 6.48 VYY2595 pKa = 10.38 DD2596 pKa = 4.9 AFGKK2600 pKa = 9.72 PGPFLNLTSLKK2611 pKa = 9.85 WGDD2614 pKa = 4.3 EE2615 pKa = 4.23 IIIHH2619 pKa = 5.72 YY2620 pKa = 10.31 GGLRR2624 pKa = 11.84 YY2625 pKa = 9.83 VYY2627 pKa = 9.55 EE2628 pKa = 3.95 VRR2630 pKa = 11.84 EE2631 pKa = 4.08 VQRR2634 pKa = 11.84 FLSADD2639 pKa = 3.45 NFSVLQSEE2647 pKa = 4.4 DD2648 pKa = 4.22 FPWVTLITCQGYY2660 pKa = 9.3 DD2661 pKa = 3.42 EE2662 pKa = 4.82 KK2663 pKa = 10.65 TDD2665 pKa = 3.51 TYY2667 pKa = 10.07 HH2668 pKa = 5.68 WRR2670 pKa = 11.84 SAVRR2674 pKa = 11.84 AVQTRR2679 pKa = 11.84 IEE2681 pKa = 4.12 EE2682 pKa = 4.11
Molecular weight: 272.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.696
IPC2_protein 3.821
IPC_protein 3.872
Toseland 3.643
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.694
Grimsley 3.541
Solomon 3.859
Lehninger 3.821
Nozaki 3.961
DTASelect 4.24
Thurlkill 3.681
EMBOSS 3.808
Sillero 3.986
Patrickios 1.583
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.883
Protein with the highest isoelectric point:
>tr|A0A0N8GNE9|A0A0N8GNE9_9CHLR Cyclic pyranopterin monophosphate synthase OS=Ornatilinea apprima OX=1134406 GN=moaC PE=3 SV=1
MM1 pKa = 7.64 PALLAHH7 pKa = 7.2 RR8 pKa = 11.84 LHH10 pKa = 6.88 HH11 pKa = 6.63 LLTRR15 pKa = 11.84 KK16 pKa = 8.26 PAPRR20 pKa = 11.84 PSPEE24 pKa = 4.3 PPPSRR29 pKa = 11.84 LPKK32 pKa = 9.85 RR33 pKa = 11.84 RR34 pKa = 11.84 PIPPPIRR41 pKa = 11.84 LPTRR45 pKa = 11.84 LPTRR49 pKa = 11.84 PPTRR53 pKa = 11.84 PPTPLRR59 pKa = 11.84 RR60 pKa = 11.84 RR61 pKa = 11.84 IPSHH65 pKa = 5.37 QRR67 pKa = 11.84 PPIPPPIRR75 pKa = 11.84 LPPLPPLRR83 pKa = 11.84 RR84 pKa = 11.84 CHH86 pKa = 5.29 WRR88 pKa = 11.84 AKK90 pKa = 8.56 RR91 pKa = 11.84 HH92 pKa = 5.91 HH93 pKa = 7.31 PSTHH97 pKa = 6.69 PLRR100 pKa = 11.84 TCPVRR105 pKa = 11.84 SLIVNWKK112 pKa = 9.46
Molecular weight: 13.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.404
IPC2_protein 10.862
IPC_protein 12.471
Toseland 12.632
ProMoST 13.13
Dawson 12.632
Bjellqvist 12.632
Wikipedia 13.115
Rodwell 12.223
Grimsley 12.676
Solomon 13.13
Lehninger 13.027
Nozaki 12.632
DTASelect 12.632
Thurlkill 12.632
EMBOSS 13.13
Sillero 12.632
Patrickios 11.945
IPC_peptide 13.13
IPC2_peptide 12.12
IPC2.peptide.svr19 9.135
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3344
0
3344
1098332
37
2682
328.4
36.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.769 ± 0.06
0.877 ± 0.014
4.956 ± 0.041
6.341 ± 0.048
4.022 ± 0.029
7.432 ± 0.04
1.871 ± 0.02
5.997 ± 0.039
3.767 ± 0.033
11.086 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.534 ± 0.021
3.454 ± 0.032
5.069 ± 0.031
4.416 ± 0.032
5.729 ± 0.04
5.879 ± 0.034
5.14 ± 0.049
7.205 ± 0.037
1.53 ± 0.02
2.927 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here