Ornatilinea apprima

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; Ornatilinea

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3344 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0P6XD38|A0A0P6XD38_9CHLR Glycerol-3-phosphate acyltransferase OS=Ornatilinea apprima OX=1134406 GN=plsY PE=3 SV=1
MM1 pKa = 7.22HH2 pKa = 7.73RR3 pKa = 11.84SFHH6 pKa = 6.56CLLTACLLIGLFSPFAAAPVLAQTPSADD34 pKa = 2.92VSVAVEE40 pKa = 4.69FSPSTIALKK49 pKa = 10.59SVSRR53 pKa = 11.84LTFTVTNANATHH65 pKa = 7.47DD66 pKa = 3.95LTGLAFSNTFPAGLSLADD84 pKa = 4.24PVNLAQTGCGGASITANPGEE104 pKa = 4.32SSISLSSGVVASGGSCAVTVSVQPSTSGNKK134 pKa = 8.33TVTVAAEE141 pKa = 4.15DD142 pKa = 3.5VTGYY146 pKa = 10.45IDD148 pKa = 4.01GSNVSNEE155 pKa = 3.64AAASDD160 pKa = 4.18EE161 pKa = 4.34LSVATFDD168 pKa = 4.35YY169 pKa = 10.77NAEE172 pKa = 3.99INKK175 pKa = 8.83QFNPISISPGDD186 pKa = 3.56ISRR189 pKa = 11.84LSVSIYY195 pKa = 9.92NANSFDD201 pKa = 3.94LTTASWTDD209 pKa = 3.26NLEE212 pKa = 4.08GVQPGLYY219 pKa = 9.35IANPPNVSTSNCGTAPEE236 pKa = 4.23VTAVAGSSTLTLANATVPAKK256 pKa = 10.46VGEE259 pKa = 4.25INGVCTVEE267 pKa = 4.11VDD269 pKa = 3.64VSSITPGNLINTIAGGNLSATGNLGTVSNTDD300 pKa = 3.54PASATINVDD309 pKa = 4.03TIQAPSVTKK318 pKa = 10.66AFAPNTIWVGQTSRR332 pKa = 11.84LTITIRR338 pKa = 11.84NNDD341 pKa = 3.16SSHH344 pKa = 6.26NLTEE348 pKa = 3.94VSLADD353 pKa = 3.6VLNDD357 pKa = 3.9PEE359 pKa = 4.12PHH361 pKa = 5.75NVVLASTVSPSLSNCGGSAVLTAVAGTDD389 pKa = 3.22NLSIANAQISPNTTCSIAVNVTSFTQGEE417 pKa = 4.51YY418 pKa = 10.69TNTIPASAVQTRR430 pKa = 11.84QGVTNATPAQANLNVQAVGLVKK452 pKa = 10.54SFSPTTILAGEE463 pKa = 4.46ISTLTITLRR472 pKa = 11.84NPTGTPYY479 pKa = 10.51TGVALDD485 pKa = 4.3DD486 pKa = 4.17VMPGAALYY494 pKa = 8.33VTGTPTTTCVGGSVSIATTSRR515 pKa = 11.84TDD517 pKa = 3.09DD518 pKa = 3.55TVRR521 pKa = 11.84LTGATVPSGTVSSPGTCTITAQITTPDD548 pKa = 3.77TILTSSHH555 pKa = 6.45LNEE558 pKa = 4.52IPAGSLSSDD567 pKa = 3.31VPGFTNVLPASATLNVQPLSIGVVKK592 pKa = 9.81TFSPTSFEE600 pKa = 4.05QGGQSTLIVTLQNSTSSPLHH620 pKa = 4.54VTQLVDD626 pKa = 3.6NLPSGLVPDD635 pKa = 4.92PATVATTCANGQAVAVVGSPSTLTLSGLDD664 pKa = 3.37ASGAAIPSGTVSVPGTCTFSVKK686 pKa = 8.04VTAPANASYY695 pKa = 11.11TNTINANSITTVEE708 pKa = 4.12GLTNLSSASRR718 pKa = 11.84SVTVYY723 pKa = 8.38PTGQGMTGSSKK734 pKa = 9.62TFSPDD739 pKa = 2.87IISAGGNSRR748 pKa = 11.84LRR750 pKa = 11.84IYY752 pKa = 9.61LTAPADD758 pKa = 3.67EE759 pKa = 5.9AITGLTVTDD768 pKa = 3.8VLPTNITISNSTAASNSCGGTLTANTGEE796 pKa = 4.28GTITLTGGSIAMGGNCTIDD815 pKa = 3.44VYY817 pKa = 10.37VTSSVSGLYY826 pKa = 10.86SNTINAATQISNDD839 pKa = 3.25QGQRR843 pKa = 11.84PPNNLIDD850 pKa = 5.05DD851 pKa = 4.3LTVSNMTISKK861 pKa = 10.32AFYY864 pKa = 8.87PSQVAPGGRR873 pKa = 11.84STLTITLRR881 pKa = 11.84NTNTAPLINVAITQDD896 pKa = 3.44NLNTMGGTSFTVANPSNKK914 pKa = 8.82STTCGGTVTADD925 pKa = 3.09SGSQIISLSGGSVPAQVGGIAGICTVSVDD954 pKa = 4.01VVSSPSTTPGDD965 pKa = 3.7KK966 pKa = 10.79TNTLYY971 pKa = 9.44RR972 pKa = 11.84TNVSGTIQGAGTVIRR987 pKa = 11.84PVANATAQLTVSSLTLGVVKK1007 pKa = 10.57GFDD1010 pKa = 3.64PLTVFGGSSSTMSVQLINPNNTPLTGIAFTDD1041 pKa = 3.61SMPTGMYY1048 pKa = 9.06VANPADD1054 pKa = 3.89VSTGTCGGTLTAVPGAGSFSFSGGSLAANKK1084 pKa = 10.19RR1085 pKa = 11.84CTLTLSVTMNVNGNRR1100 pKa = 11.84TNTIPAEE1107 pKa = 4.24GVTSFNGAKK1116 pKa = 10.16NSQPAQASLTNLPGVSISKK1135 pKa = 10.03YY1136 pKa = 9.43FAPTSMILGEE1146 pKa = 4.26TSLLTIQIKK1155 pKa = 8.38NTGNIALSNLGMVDD1169 pKa = 3.68TLPAGLTIASSPSPTNACNGLLTAAPGTNTISLGAGSIAPGPNTTCNITVPVTASAAGTYY1229 pKa = 10.91NNTIGKK1235 pKa = 8.07NTVTTAEE1242 pKa = 4.48GATNTEE1248 pKa = 4.29PATDD1252 pKa = 3.61TLTVNAAPTMQMVKK1266 pKa = 9.21TFDD1269 pKa = 3.4VAGSSEE1275 pKa = 4.0APYY1278 pKa = 10.66TDD1280 pKa = 4.18GDD1282 pKa = 3.95TLAYY1286 pKa = 9.42TLTATNTGDD1295 pKa = 3.39VPLTGVEE1302 pKa = 3.8ISDD1305 pKa = 3.79PGVVLGTCTPPQPASLAPGEE1325 pKa = 4.78SLTCPATHH1333 pKa = 6.91IVTADD1338 pKa = 3.23EE1339 pKa = 4.41AAAGSYY1345 pKa = 10.35TNTASADD1352 pKa = 3.49SDD1354 pKa = 3.86QIGPVTDD1361 pKa = 3.62SVTVPTDD1368 pKa = 2.97QTNILGISKK1377 pKa = 10.52VITTAGPYY1385 pKa = 10.18EE1386 pKa = 4.62LGDD1389 pKa = 3.61TLNYY1393 pKa = 9.14TITVTNIGSGTLNNVGVADD1412 pKa = 4.03PGMVLGACTPPQPATLTSGQSMSCPASHH1440 pKa = 6.82VVTQADD1446 pKa = 3.21VDD1448 pKa = 3.84AGTFTNTATGDD1459 pKa = 3.51SSEE1462 pKa = 4.34TDD1464 pKa = 3.3PVTDD1468 pKa = 3.45SVTVEE1473 pKa = 3.78IKK1475 pKa = 10.83DD1476 pKa = 3.57NARR1479 pKa = 11.84LEE1481 pKa = 4.4VYY1483 pKa = 10.27KK1484 pKa = 10.54QVTSNGPYY1492 pKa = 8.11NTVGKK1497 pKa = 8.64TITYY1501 pKa = 9.41DD1502 pKa = 3.1ISAINTGDD1510 pKa = 3.32QTLTGVTITDD1520 pKa = 3.89PGTGVTLGACTPAQPATLTTGQILSCAASHH1550 pKa = 7.35DD1551 pKa = 3.84VTADD1555 pKa = 4.09DD1556 pKa = 3.45ITAGGFSNTAYY1567 pKa = 10.44ADD1569 pKa = 3.62SDD1571 pKa = 3.53QTSPVSSTAEE1581 pKa = 4.21VITQTPKK1588 pKa = 10.19IDD1590 pKa = 3.57LAKK1593 pKa = 10.27TGTLNLGANGRR1604 pKa = 11.84ADD1606 pKa = 3.77AGDD1609 pKa = 4.0TITYY1613 pKa = 10.22AFTVTNTGEE1622 pKa = 4.07VTLNNITLIDD1632 pKa = 3.52IVGGVSISGGPIASLAPLASDD1653 pKa = 3.09STTFTGSYY1661 pKa = 9.36TLTQADD1667 pKa = 3.77VNAGNFTNTASVTGAPPVGNIVSDD1691 pKa = 3.93TDD1693 pKa = 3.62NDD1695 pKa = 4.29TQTLTPAASIDD1706 pKa = 3.81LQKK1709 pKa = 10.1TGTLNLNASAPNGIANPGDD1728 pKa = 3.79TISYY1732 pKa = 10.34AFTVTNTGNVTLTNITLADD1751 pKa = 3.77TVGGISISGGPIASLAPGASNSSTFTGSYY1780 pKa = 9.33TLNQTNVDD1788 pKa = 3.61AGTFTNTATVTGTPPSGPNVTDD1810 pKa = 4.87ADD1812 pKa = 5.11DD1813 pKa = 4.06DD1814 pKa = 4.15TQTIIPVPSIEE1825 pKa = 4.08LVKK1828 pKa = 10.33TGTLNLGANSRR1839 pKa = 11.84ADD1841 pKa = 3.43AGDD1844 pKa = 3.84TITYY1848 pKa = 10.22AFTVTNTGNVTLSGVTLSDD1867 pKa = 3.49PKK1869 pKa = 9.79ITEE1872 pKa = 3.82SGGPITLAPGASNSTAFTGTYY1893 pKa = 9.49TLTQADD1899 pKa = 4.07VNAGTFTNTATVTGTTPGGPTVNDD1923 pKa = 3.56SDD1925 pKa = 5.88GDD1927 pKa = 4.14TQTLDD1932 pKa = 3.7PAPSVALQKK1941 pKa = 9.88TGTLNDD1947 pKa = 4.32GPDD1950 pKa = 3.69GEE1952 pKa = 4.57PDD1954 pKa = 2.98EE1955 pKa = 5.77GEE1957 pKa = 3.89IITYY1961 pKa = 9.4VFAVTNTGNVTLTNLTVTEE1980 pKa = 4.32TVGGITLTGSPIASLEE1996 pKa = 4.06PGASNNTNYY2005 pKa = 9.63TGSYY2009 pKa = 9.37TLTQEE2014 pKa = 4.63VIDD2017 pKa = 4.84SGTFTNTAQVSGTPPSGSPVTDD2039 pKa = 3.58SDD2041 pKa = 6.15DD2042 pKa = 3.7DD2043 pKa = 4.14TQLLTDD2049 pKa = 4.04VPAITLEE2056 pKa = 4.16KK2057 pKa = 10.21TGTLNMDD2064 pKa = 3.68VVAPDD2069 pKa = 3.64DD2070 pKa = 3.86RR2071 pKa = 11.84ADD2073 pKa = 3.4VGDD2076 pKa = 4.24TIRR2079 pKa = 11.84YY2080 pKa = 8.71AFTVSNTGNVTLTDD2094 pKa = 3.07ITLADD2099 pKa = 3.75TVGGITLAGGPITSLDD2115 pKa = 4.0PDD2117 pKa = 4.82DD2118 pKa = 5.61SDD2120 pKa = 5.07SDD2122 pKa = 4.14TFTGVYY2128 pKa = 9.46TLTQADD2134 pKa = 3.42IDD2136 pKa = 4.2AGTFTNTAEE2145 pKa = 4.44VTATPPEE2152 pKa = 4.65GDD2154 pKa = 4.42DD2155 pKa = 3.57VTGSDD2160 pKa = 5.98DD2161 pKa = 3.75DD2162 pKa = 4.24TQTLAEE2168 pKa = 4.06TARR2171 pKa = 11.84IGVAKK2176 pKa = 10.15RR2177 pKa = 11.84VTGSPAEE2184 pKa = 4.02ITPGVWDD2191 pKa = 3.62VTFEE2195 pKa = 4.0ILVRR2199 pKa = 11.84NYY2201 pKa = 11.32GNVTLSSIQVTDD2213 pKa = 3.85DD2214 pKa = 3.75LSAVFPPEE2222 pKa = 3.75SPFAVQSLTSDD2233 pKa = 3.41DD2234 pKa = 3.86FTVNSAYY2241 pKa = 10.36DD2242 pKa = 3.37GRR2244 pKa = 11.84TDD2246 pKa = 4.72ANLLDD2251 pKa = 4.55GTDD2254 pKa = 3.26SLAFGEE2260 pKa = 4.39QGSITLVVRR2269 pKa = 11.84VTPADD2274 pKa = 3.6GGPFNNTAAASAISPSEE2291 pKa = 4.2SPVSDD2296 pKa = 3.46TSHH2299 pKa = 6.76NGANPDD2305 pKa = 3.78PDD2307 pKa = 4.13YY2308 pKa = 11.74DD2309 pKa = 5.5GDD2311 pKa = 3.93PTDD2314 pKa = 5.04NNDD2317 pKa = 3.32PTPVDD2322 pKa = 4.57FGADD2326 pKa = 3.52LFDD2329 pKa = 4.27PPYY2332 pKa = 10.66GIKK2335 pKa = 9.72TYY2337 pKa = 11.02DD2338 pKa = 3.48SFGMPLLQWTMVWINNSNIVNVGARR2363 pKa = 11.84VSDD2366 pKa = 5.18PIPSGAVYY2374 pKa = 9.34EE2375 pKa = 4.32ASGLPSGTGIPDD2387 pKa = 4.25GAPAGSTDD2395 pKa = 3.18VGVVCNPLGIIDD2407 pKa = 4.48PKK2409 pKa = 10.47EE2410 pKa = 3.65ISPTISLSATFTTWCYY2426 pKa = 11.25YY2427 pKa = 10.08EE2428 pKa = 5.75GPTLTYY2434 pKa = 9.54PRR2436 pKa = 11.84GRR2438 pKa = 11.84IVWEE2442 pKa = 4.34GALGPDD2448 pKa = 5.26LGITDD2453 pKa = 3.9PALAEE2458 pKa = 4.12NEE2460 pKa = 3.91LTITFNLRR2468 pKa = 11.84VLEE2471 pKa = 4.41GEE2473 pKa = 4.13HH2474 pKa = 6.7EE2475 pKa = 4.3INNRR2479 pKa = 11.84ATIDD2483 pKa = 3.1VDD2485 pKa = 4.02RR2486 pKa = 11.84NGNGLIEE2493 pKa = 4.72GEE2495 pKa = 4.6TEE2497 pKa = 3.94VEE2499 pKa = 3.88AASATEE2505 pKa = 4.2TWSNWPEE2512 pKa = 3.91ALPLTGFAPGKK2523 pKa = 9.0ITDD2526 pKa = 3.7LSGRR2530 pKa = 11.84PWVEE2534 pKa = 3.26YY2535 pKa = 10.54DD2536 pKa = 3.46QSQDD2540 pKa = 3.49LLLEE2544 pKa = 4.32IPALNVKK2551 pKa = 9.74VPILGVPIVHH2561 pKa = 6.74GEE2563 pKa = 3.95WNLDD2567 pKa = 3.31WLGGNAGYY2575 pKa = 10.71LEE2577 pKa = 4.13NTAFPTHH2584 pKa = 6.87AGNTGITAHH2593 pKa = 6.48VYY2595 pKa = 10.38DD2596 pKa = 4.9AFGKK2600 pKa = 9.72PGPFLNLTSLKK2611 pKa = 9.85WGDD2614 pKa = 4.3EE2615 pKa = 4.23IIIHH2619 pKa = 5.72YY2620 pKa = 10.31GGLRR2624 pKa = 11.84YY2625 pKa = 9.83VYY2627 pKa = 9.55EE2628 pKa = 3.95VRR2630 pKa = 11.84EE2631 pKa = 4.08VQRR2634 pKa = 11.84FLSADD2639 pKa = 3.45NFSVLQSEE2647 pKa = 4.4DD2648 pKa = 4.22FPWVTLITCQGYY2660 pKa = 9.3DD2661 pKa = 3.42EE2662 pKa = 4.82KK2663 pKa = 10.65TDD2665 pKa = 3.51TYY2667 pKa = 10.07HH2668 pKa = 5.68WRR2670 pKa = 11.84SAVRR2674 pKa = 11.84AVQTRR2679 pKa = 11.84IEE2681 pKa = 4.12EE2682 pKa = 4.11

Molecular weight:
272.35 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0N8GNE9|A0A0N8GNE9_9CHLR Cyclic pyranopterin monophosphate synthase OS=Ornatilinea apprima OX=1134406 GN=moaC PE=3 SV=1
MM1 pKa = 7.64PALLAHH7 pKa = 7.2RR8 pKa = 11.84LHH10 pKa = 6.88HH11 pKa = 6.63LLTRR15 pKa = 11.84KK16 pKa = 8.26PAPRR20 pKa = 11.84PSPEE24 pKa = 4.3PPPSRR29 pKa = 11.84LPKK32 pKa = 9.85RR33 pKa = 11.84RR34 pKa = 11.84PIPPPIRR41 pKa = 11.84LPTRR45 pKa = 11.84LPTRR49 pKa = 11.84PPTRR53 pKa = 11.84PPTPLRR59 pKa = 11.84RR60 pKa = 11.84RR61 pKa = 11.84IPSHH65 pKa = 5.37QRR67 pKa = 11.84PPIPPPIRR75 pKa = 11.84LPPLPPLRR83 pKa = 11.84RR84 pKa = 11.84CHH86 pKa = 5.29WRR88 pKa = 11.84AKK90 pKa = 8.56RR91 pKa = 11.84HH92 pKa = 5.91HH93 pKa = 7.31PSTHH97 pKa = 6.69PLRR100 pKa = 11.84TCPVRR105 pKa = 11.84SLIVNWKK112 pKa = 9.46

Molecular weight:
13.08 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3344

0

3344

1098332

37

2682

328.4

36.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.769 ± 0.06

0.877 ± 0.014

4.956 ± 0.041

6.341 ± 0.048

4.022 ± 0.029

7.432 ± 0.04

1.871 ± 0.02

5.997 ± 0.039

3.767 ± 0.033

11.086 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.534 ± 0.021

3.454 ± 0.032

5.069 ± 0.031

4.416 ± 0.032

5.729 ± 0.04

5.879 ± 0.034

5.14 ± 0.049

7.205 ± 0.037

1.53 ± 0.02

2.927 ± 0.024

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski