Rhodococcus tukisamuensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4987 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G7BGF2|A0A1G7BGF2_9NOCA Uncharacterized protein OS=Rhodococcus tukisamuensis OX=168276 GN=SAMN05444580_11321 PE=4 SV=1
MM1 pKa = 7.62TEE3 pKa = 5.51LDD5 pKa = 3.93SQDD8 pKa = 3.12QLLRR12 pKa = 11.84IDD14 pKa = 4.34GEE16 pKa = 4.19LAIATEE22 pKa = 4.58GHH24 pKa = 6.43DD25 pKa = 3.94QLHH28 pKa = 6.2ALCLRR33 pKa = 11.84TLAEE37 pKa = 4.44LLGSEE42 pKa = 4.71PDD44 pKa = 3.25VDD46 pKa = 3.97EE47 pKa = 6.24DD48 pKa = 4.12GDD50 pKa = 3.85IAIPVHH56 pKa = 6.02GFVVYY61 pKa = 8.04VTVADD66 pKa = 5.23DD67 pKa = 4.55GPQLHH72 pKa = 6.26VWSSILTGLGDD83 pKa = 3.43RR84 pKa = 11.84ARR86 pKa = 11.84AAEE89 pKa = 4.29ALTDD93 pKa = 5.57LALEE97 pKa = 4.29WPRR100 pKa = 11.84LRR102 pKa = 11.84FVFSDD107 pKa = 3.24DD108 pKa = 3.58HH109 pKa = 8.24LLVSTILDD117 pKa = 3.81AEE119 pKa = 4.34PFAPQHH125 pKa = 6.42LINLVDD131 pKa = 3.89EE132 pKa = 4.44MHH134 pKa = 6.87EE135 pKa = 4.22LTHH138 pKa = 7.62DD139 pKa = 4.62LDD141 pKa = 5.67DD142 pKa = 5.03DD143 pKa = 3.94LAARR147 pKa = 11.84FGGTLDD153 pKa = 4.98CDD155 pKa = 4.17TEE157 pKa = 5.0DD158 pKa = 3.66DD159 pKa = 4.71SYY161 pKa = 12.19AGGCGGCGDD170 pKa = 5.71DD171 pKa = 5.44CDD173 pKa = 4.99CDD175 pKa = 5.29CGDD178 pKa = 3.94TDD180 pKa = 5.06EE181 pKa = 4.57GHH183 pKa = 7.52LDD185 pKa = 3.13ITAVNPSKK193 pKa = 11.14

Molecular weight: 20.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G7B447|A0A1G7B447_9NOCA L-lactate dehydrogenase complex protein LldG OS=Rhodococcus tukisamuensis OX=168276 GN=SAMN05444580_11263 PE=4 SV=1
MM1 pKa = 8.07DD2 pKa = 4.41PTASTDD8 pKa = 3.29RR9 pKa = 11.84RR10 pKa = 11.84DD11 pKa = 3.39AAVATVTDD19 pKa = 4.67FFAALDD25 pKa = 3.93ALDD28 pKa = 3.75VPRR31 pKa = 11.84AGTFLTPDD39 pKa = 3.56VVWRR43 pKa = 11.84NTTLPTLRR51 pKa = 11.84GLPTVTRR58 pKa = 11.84ALRR61 pKa = 11.84AAARR65 pKa = 11.84PRR67 pKa = 11.84CRR69 pKa = 11.84FEE71 pKa = 4.86AVVHH75 pKa = 6.45HH76 pKa = 6.71IASDD80 pKa = 3.5GDD82 pKa = 3.7AVLVEE87 pKa = 4.33RR88 pKa = 11.84TDD90 pKa = 3.55TLTLGPLRR98 pKa = 11.84STFWVCGTFEE108 pKa = 4.18IRR110 pKa = 11.84DD111 pKa = 3.51GKK113 pKa = 9.33IAVWRR118 pKa = 11.84DD119 pKa = 3.09YY120 pKa = 11.03FSWRR124 pKa = 11.84NVLLGAVTGTLRR136 pKa = 11.84SVRR139 pKa = 11.84GRR141 pKa = 11.84QRR143 pKa = 11.84HH144 pKa = 5.33LPAAPGHH151 pKa = 5.7GRR153 pKa = 11.84STGTIPGAAPATVPPDD169 pKa = 3.71YY170 pKa = 10.3PRR172 pKa = 11.84KK173 pKa = 9.88DD174 pKa = 3.34QVKK177 pKa = 9.87

Molecular weight: 19.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4987

0

4987

1634117

29

9173

327.7

34.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.428 ± 0.047

0.747 ± 0.011

6.2 ± 0.03

5.432 ± 0.035

2.964 ± 0.021

9.336 ± 0.037

2.055 ± 0.018

3.686 ± 0.026

1.983 ± 0.029

10.098 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.941 ± 0.015

2.005 ± 0.02

5.773 ± 0.031

2.736 ± 0.016

7.272 ± 0.04

5.466 ± 0.025

6.214 ± 0.032

9.215 ± 0.041

1.46 ± 0.014

1.99 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski