Paenibacillus phage Fern

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fernvirus; Paenibacillus virus Fern

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K2CXV9|A0A0K2CXV9_9CAUD Uncharacterized protein OS=Paenibacillus phage Fern OX=1636255 GN=FERN_63 PE=4 SV=1
MM1 pKa = 7.16QNYY4 pKa = 8.86GFLGEE9 pKa = 4.03MVANSFCQDD18 pKa = 3.31LCNVFIDD25 pKa = 4.16NFMNNQFIPTNYY37 pKa = 9.04TQFLL41 pKa = 3.8

Molecular weight: 4.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K2CXN8|A0A0K2CXN8_9CAUD Terminase small subunit OS=Paenibacillus phage Fern OX=1636255 GN=FERN_1 PE=4 SV=1
MM1 pKa = 8.08RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84DD5 pKa = 3.66KK6 pKa = 11.3NAMINSYY13 pKa = 10.06FLSFLEE19 pKa = 5.07QVTCSKK25 pKa = 10.85KK26 pKa = 8.9ITLATLLRR34 pKa = 11.84LRR36 pKa = 11.84GFFYY40 pKa = 10.62KK41 pKa = 10.4QLTRR45 pKa = 11.84FCHH48 pKa = 5.24VFF50 pKa = 3.09

Molecular weight: 6.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

11799

41

878

173.5

19.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.45 ± 0.525

0.983 ± 0.173

5.772 ± 0.214

8.509 ± 0.419

3.788 ± 0.181

6.102 ± 0.354

1.822 ± 0.204

7.102 ± 0.264

8.891 ± 0.361

8.068 ± 0.267

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.992 ± 0.18

4.712 ± 0.232

3.39 ± 0.206

4.043 ± 0.219

4.721 ± 0.37

5.95 ± 0.333

5.458 ± 0.299

6.085 ± 0.235

1.458 ± 0.183

3.704 ± 0.268
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski