Amino acid dipepetide frequency for Paenibacillus phage Fern

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.747AlaAla: 4.747 ± 1.083
0.593AlaCys: 0.593 ± 0.171
4.577AlaAsp: 4.577 ± 0.655
6.527AlaGlu: 6.527 ± 0.732
1.695AlaPhe: 1.695 ± 0.42
4.408AlaGly: 4.408 ± 0.679
0.848AlaHis: 0.848 ± 0.252
4.408AlaIle: 4.408 ± 0.851
5.34AlaLys: 5.34 ± 0.716
5.509AlaLeu: 5.509 ± 0.664
1.356AlaMet: 1.356 ± 0.343
3.221AlaAsn: 3.221 ± 0.536
1.526AlaPro: 1.526 ± 0.404
1.949AlaGln: 1.949 ± 0.39
1.695AlaArg: 1.695 ± 0.331
3.729AlaSer: 3.729 ± 0.63
4.238AlaThr: 4.238 ± 0.796
4.238AlaVal: 4.238 ± 0.51
1.356AlaTrp: 1.356 ± 0.363
2.543AlaTyr: 2.543 ± 0.437
0.0AlaXaa: 0.0 ± 0.0
Cys
0.339CysAla: 0.339 ± 0.148
0.339CysCys: 0.339 ± 0.222
0.509CysAsp: 0.509 ± 0.196
0.678CysGlu: 0.678 ± 0.283
0.339CysPhe: 0.339 ± 0.178
0.593CysGly: 0.593 ± 0.213
0.339CysHis: 0.339 ± 0.162
0.339CysIle: 0.339 ± 0.161
1.271CysLys: 1.271 ± 0.441
1.102CysLeu: 1.102 ± 0.281
0.424CysMet: 0.424 ± 0.176
0.339CysAsn: 0.339 ± 0.156
0.424CysPro: 0.424 ± 0.229
0.339CysGln: 0.339 ± 0.154
0.339CysArg: 0.339 ± 0.139
0.593CysSer: 0.593 ± 0.217
0.339CysThr: 0.339 ± 0.165
0.509CysVal: 0.509 ± 0.194
0.254CysTrp: 0.254 ± 0.212
0.424CysTyr: 0.424 ± 0.179
0.0CysXaa: 0.0 ± 0.0
Asp
3.645AspAla: 3.645 ± 0.786
0.339AspCys: 0.339 ± 0.163
3.645AspAsp: 3.645 ± 0.731
4.153AspGlu: 4.153 ± 0.731
3.814AspPhe: 3.814 ± 0.509
4.323AspGly: 4.323 ± 0.586
0.848AspHis: 0.848 ± 0.235
3.729AspIle: 3.729 ± 0.453
3.814AspLys: 3.814 ± 0.638
5.001AspLeu: 5.001 ± 0.612
2.458AspMet: 2.458 ± 0.499
1.949AspAsn: 1.949 ± 0.398
2.119AspPro: 2.119 ± 0.416
1.949AspGln: 1.949 ± 0.453
2.628AspArg: 2.628 ± 0.433
2.712AspSer: 2.712 ± 0.477
3.306AspThr: 3.306 ± 0.429
4.238AspVal: 4.238 ± 0.509
0.848AspTrp: 0.848 ± 0.253
2.204AspTyr: 2.204 ± 0.406
0.0AspXaa: 0.0 ± 0.0
Glu
5.848GluAla: 5.848 ± 0.664
0.424GluCys: 0.424 ± 0.229
5.17GluAsp: 5.17 ± 0.806
6.527GluGlu: 6.527 ± 0.82
2.628GluPhe: 2.628 ± 0.403
3.729GluGly: 3.729 ± 0.429
1.271GluHis: 1.271 ± 0.349
6.357GluIle: 6.357 ± 0.804
8.052GluLys: 8.052 ± 0.834
7.035GluLeu: 7.035 ± 0.727
4.577GluMet: 4.577 ± 0.587
3.899GluAsn: 3.899 ± 0.682
2.458GluPro: 2.458 ± 0.45
4.068GluGln: 4.068 ± 0.782
4.238GluArg: 4.238 ± 0.724
4.153GluSer: 4.153 ± 0.569
4.831GluThr: 4.831 ± 0.569
5.425GluVal: 5.425 ± 0.685
1.017GluTrp: 1.017 ± 0.271
3.39GluTyr: 3.39 ± 0.5
0.0GluXaa: 0.0 ± 0.0
Phe
1.949PheAla: 1.949 ± 0.327
1.017PheCys: 1.017 ± 0.262
2.543PheAsp: 2.543 ± 0.462
3.56PheGlu: 3.56 ± 0.583
1.102PhePhe: 1.102 ± 0.324
2.204PheGly: 2.204 ± 0.351
1.187PheHis: 1.187 ± 0.378
3.221PheIle: 3.221 ± 0.496
2.967PheLys: 2.967 ± 0.615
2.543PheLeu: 2.543 ± 0.415
1.356PheMet: 1.356 ± 0.411
2.119PheAsn: 2.119 ± 0.398
0.848PhePro: 0.848 ± 0.251
1.78PheGln: 1.78 ± 0.402
1.102PheArg: 1.102 ± 0.324
2.458PheSer: 2.458 ± 0.44
1.441PheThr: 1.441 ± 0.336
2.712PheVal: 2.712 ± 0.543
0.339PheTrp: 0.339 ± 0.138
1.441PheTyr: 1.441 ± 0.337
0.0PheXaa: 0.0 ± 0.0
Gly
2.967GlyAla: 2.967 ± 0.533
1.017GlyCys: 1.017 ± 0.361
3.306GlyAsp: 3.306 ± 0.5
5.848GlyGlu: 5.848 ± 0.803
2.712GlyPhe: 2.712 ± 0.41
2.712GlyGly: 2.712 ± 0.495
0.848GlyHis: 0.848 ± 0.269
5.848GlyIle: 5.848 ± 0.997
6.696GlyLys: 6.696 ± 0.752
4.831GlyLeu: 4.831 ± 0.905
1.865GlyMet: 1.865 ± 0.608
3.221GlyAsn: 3.221 ± 0.482
0.932GlyPro: 0.932 ± 0.395
2.458GlyGln: 2.458 ± 0.455
3.051GlyArg: 3.051 ± 0.501
3.39GlySer: 3.39 ± 0.587
2.289GlyThr: 2.289 ± 0.457
3.729GlyVal: 3.729 ± 0.56
1.102GlyTrp: 1.102 ± 0.225
2.204GlyTyr: 2.204 ± 0.389
0.0GlyXaa: 0.0 ± 0.0
His
1.271HisAla: 1.271 ± 0.381
0.254HisCys: 0.254 ± 0.159
0.509HisAsp: 0.509 ± 0.212
1.271HisGlu: 1.271 ± 0.324
0.848HisPhe: 0.848 ± 0.274
1.017HisGly: 1.017 ± 0.293
0.339HisHis: 0.339 ± 0.16
2.119HisIle: 2.119 ± 0.408
1.017HisLys: 1.017 ± 0.307
2.204HisLeu: 2.204 ± 0.49
0.424HisMet: 0.424 ± 0.192
1.356HisAsn: 1.356 ± 0.285
0.763HisPro: 0.763 ± 0.259
0.593HisGln: 0.593 ± 0.211
1.102HisArg: 1.102 ± 0.3
0.932HisSer: 0.932 ± 0.314
0.509HisThr: 0.509 ± 0.24
1.187HisVal: 1.187 ± 0.294
0.17HisTrp: 0.17 ± 0.108
0.339HisTyr: 0.339 ± 0.143
0.0HisXaa: 0.0 ± 0.0
Ile
3.899IleAla: 3.899 ± 0.634
1.017IleCys: 1.017 ± 0.313
4.747IleAsp: 4.747 ± 0.683
7.12IleGlu: 7.12 ± 0.79
3.051IlePhe: 3.051 ± 0.398
3.899IleGly: 3.899 ± 0.564
1.78IleHis: 1.78 ± 0.359
3.051IleIle: 3.051 ± 0.689
7.205IleLys: 7.205 ± 1.081
5.34IleLeu: 5.34 ± 0.543
1.187IleMet: 1.187 ± 0.33
3.814IleAsn: 3.814 ± 0.63
2.712IlePro: 2.712 ± 0.468
2.967IleGln: 2.967 ± 0.499
3.899IleArg: 3.899 ± 0.586
3.56IleSer: 3.56 ± 0.587
4.153IleThr: 4.153 ± 0.416
4.323IleVal: 4.323 ± 0.655
0.678IleTrp: 0.678 ± 0.277
2.628IleTyr: 2.628 ± 0.397
0.0IleXaa: 0.0 ± 0.0
Lys
6.611LysAla: 6.611 ± 0.601
0.339LysCys: 0.339 ± 0.17
4.068LysAsp: 4.068 ± 0.417
8.73LysGlu: 8.73 ± 0.991
2.119LysPhe: 2.119 ± 0.346
5.594LysGly: 5.594 ± 0.552
1.61LysHis: 1.61 ± 0.281
6.357LysIle: 6.357 ± 0.681
7.289LysLys: 7.289 ± 1.055
6.866LysLeu: 6.866 ± 0.857
3.221LysMet: 3.221 ± 0.386
4.068LysAsn: 4.068 ± 0.678
2.628LysPro: 2.628 ± 0.481
4.492LysGln: 4.492 ± 0.591
4.662LysArg: 4.662 ± 0.647
5.425LysSer: 5.425 ± 0.662
5.679LysThr: 5.679 ± 0.707
4.492LysVal: 4.492 ± 0.547
1.356LysTrp: 1.356 ± 0.382
3.306LysTyr: 3.306 ± 0.597
0.0LysXaa: 0.0 ± 0.0
Leu
6.272LeuAla: 6.272 ± 0.68
0.678LeuCys: 0.678 ± 0.251
4.916LeuAsp: 4.916 ± 0.645
7.798LeuGlu: 7.798 ± 0.861
2.373LeuPhe: 2.373 ± 0.434
4.662LeuGly: 4.662 ± 0.504
1.865LeuHis: 1.865 ± 0.334
5.425LeuIle: 5.425 ± 0.548
6.95LeuLys: 6.95 ± 0.746
5.255LeuLeu: 5.255 ± 0.652
1.695LeuMet: 1.695 ± 0.421
3.814LeuAsn: 3.814 ± 0.443
3.221LeuPro: 3.221 ± 0.457
3.814LeuGln: 3.814 ± 0.499
3.729LeuArg: 3.729 ± 0.55
5.34LeuSer: 5.34 ± 0.653
5.425LeuThr: 5.425 ± 0.595
3.56LeuVal: 3.56 ± 0.585
0.848LeuTrp: 0.848 ± 0.356
3.051LeuTyr: 3.051 ± 0.502
0.0LeuXaa: 0.0 ± 0.0
Met
2.204MetAla: 2.204 ± 0.427
0.0MetCys: 0.0 ± 0.0
1.695MetAsp: 1.695 ± 0.34
2.289MetGlu: 2.289 ± 0.444
1.271MetPhe: 1.271 ± 0.279
1.102MetGly: 1.102 ± 0.329
0.0MetHis: 0.0 ± 0.0
2.204MetIle: 2.204 ± 0.42
3.475MetLys: 3.475 ± 0.452
2.289MetLeu: 2.289 ± 0.514
0.763MetMet: 0.763 ± 0.22
2.628MetAsn: 2.628 ± 0.444
1.017MetPro: 1.017 ± 0.283
1.526MetGln: 1.526 ± 0.288
1.441MetArg: 1.441 ± 0.328
2.289MetSer: 2.289 ± 0.331
1.271MetThr: 1.271 ± 0.332
1.017MetVal: 1.017 ± 0.303
0.339MetTrp: 0.339 ± 0.167
1.102MetTyr: 1.102 ± 0.283
0.0MetXaa: 0.0 ± 0.0
Asn
2.543AsnAla: 2.543 ± 0.416
0.509AsnCys: 0.509 ± 0.203
2.034AsnAsp: 2.034 ± 0.428
4.153AsnGlu: 4.153 ± 0.618
1.526AsnPhe: 1.526 ± 0.431
4.068AsnGly: 4.068 ± 0.64
0.763AsnHis: 0.763 ± 0.193
3.306AsnIle: 3.306 ± 0.503
3.645AsnLys: 3.645 ± 0.538
3.984AsnLeu: 3.984 ± 0.533
1.017AsnMet: 1.017 ± 0.45
1.865AsnAsn: 1.865 ± 0.55
2.543AsnPro: 2.543 ± 0.502
1.695AsnGln: 1.695 ± 0.39
3.39AsnArg: 3.39 ± 0.57
2.797AsnSer: 2.797 ± 0.39
2.628AsnThr: 2.628 ± 0.398
2.543AsnVal: 2.543 ± 0.413
0.593AsnTrp: 0.593 ± 0.227
1.526AsnTyr: 1.526 ± 0.349
0.0AsnXaa: 0.0 ± 0.0
Pro
2.034ProAla: 2.034 ± 0.322
0.085ProCys: 0.085 ± 0.081
1.949ProAsp: 1.949 ± 0.505
3.136ProGlu: 3.136 ± 0.774
1.441ProPhe: 1.441 ± 0.324
2.289ProGly: 2.289 ± 0.373
0.932ProHis: 0.932 ± 0.295
2.712ProIle: 2.712 ± 0.442
3.136ProLys: 3.136 ± 0.584
2.458ProLeu: 2.458 ± 0.434
0.848ProMet: 0.848 ± 0.296
1.356ProAsn: 1.356 ± 0.322
1.017ProPro: 1.017 ± 0.274
0.763ProGln: 0.763 ± 0.307
1.017ProArg: 1.017 ± 0.255
2.882ProSer: 2.882 ± 0.518
1.78ProThr: 1.78 ± 0.313
2.034ProVal: 2.034 ± 0.597
0.678ProTrp: 0.678 ± 0.228
1.356ProTyr: 1.356 ± 0.3
0.0ProXaa: 0.0 ± 0.0
Gln
3.56GlnAla: 3.56 ± 0.558
0.17GlnCys: 0.17 ± 0.11
1.949GlnAsp: 1.949 ± 0.339
3.475GlnGlu: 3.475 ± 0.519
2.119GlnPhe: 2.119 ± 0.328
2.543GlnGly: 2.543 ± 0.6
1.017GlnHis: 1.017 ± 0.269
2.119GlnIle: 2.119 ± 0.471
3.221GlnLys: 3.221 ± 0.466
3.475GlnLeu: 3.475 ± 0.702
1.695GlnMet: 1.695 ± 0.457
1.695GlnAsn: 1.695 ± 0.336
1.949GlnPro: 1.949 ± 0.494
1.526GlnGln: 1.526 ± 0.431
2.034GlnArg: 2.034 ± 0.452
2.204GlnSer: 2.204 ± 0.376
2.458GlnThr: 2.458 ± 0.407
1.949GlnVal: 1.949 ± 0.418
0.339GlnTrp: 0.339 ± 0.182
0.932GlnTyr: 0.932 ± 0.248
0.0GlnXaa: 0.0 ± 0.0
Arg
2.882ArgAla: 2.882 ± 0.414
0.593ArgCys: 0.593 ± 0.222
2.967ArgAsp: 2.967 ± 0.438
3.729ArgGlu: 3.729 ± 0.622
1.356ArgPhe: 1.356 ± 0.437
2.628ArgGly: 2.628 ± 0.526
1.017ArgHis: 1.017 ± 0.301
2.882ArgIle: 2.882 ± 0.364
5.764ArgLys: 5.764 ± 0.98
4.408ArgLeu: 4.408 ± 0.605
1.187ArgMet: 1.187 ± 0.292
1.949ArgAsn: 1.949 ± 0.427
1.78ArgPro: 1.78 ± 0.36
2.373ArgGln: 2.373 ± 0.422
2.373ArgArg: 2.373 ± 0.46
2.034ArgSer: 2.034 ± 0.372
1.695ArgThr: 1.695 ± 0.31
2.967ArgVal: 2.967 ± 0.591
0.848ArgTrp: 0.848 ± 0.264
1.78ArgTyr: 1.78 ± 0.344
0.0ArgXaa: 0.0 ± 0.0
Ser
4.831SerAla: 4.831 ± 1.127
0.339SerCys: 0.339 ± 0.158
3.56SerAsp: 3.56 ± 0.585
4.662SerGlu: 4.662 ± 0.579
2.543SerPhe: 2.543 ± 0.457
4.238SerGly: 4.238 ± 0.636
0.932SerHis: 0.932 ± 0.297
5.848SerIle: 5.848 ± 0.78
4.408SerLys: 4.408 ± 0.671
4.577SerLeu: 4.577 ± 0.844
1.695SerMet: 1.695 ± 0.314
2.034SerAsn: 2.034 ± 0.362
2.119SerPro: 2.119 ± 0.437
1.949SerGln: 1.949 ± 0.455
2.628SerArg: 2.628 ± 0.474
3.814SerSer: 3.814 ± 0.838
3.306SerThr: 3.306 ± 0.55
3.645SerVal: 3.645 ± 0.829
0.424SerTrp: 0.424 ± 0.212
1.949SerTyr: 1.949 ± 0.354
0.0SerXaa: 0.0 ± 0.0
Thr
3.899ThrAla: 3.899 ± 0.606
0.424ThrCys: 0.424 ± 0.173
2.373ThrAsp: 2.373 ± 0.369
3.306ThrGlu: 3.306 ± 0.502
1.526ThrPhe: 1.526 ± 0.346
4.238ThrGly: 4.238 ± 0.511
1.187ThrHis: 1.187 ± 0.331
3.814ThrIle: 3.814 ± 0.59
4.831ThrLys: 4.831 ± 0.657
4.831ThrLeu: 4.831 ± 0.666
1.61ThrMet: 1.61 ± 0.447
1.949ThrAsn: 1.949 ± 0.376
3.051ThrPro: 3.051 ± 0.476
2.628ThrGln: 2.628 ± 0.43
2.119ThrArg: 2.119 ± 0.456
3.475ThrSer: 3.475 ± 0.524
2.204ThrThr: 2.204 ± 0.463
4.577ThrVal: 4.577 ± 0.533
0.509ThrTrp: 0.509 ± 0.283
2.034ThrTyr: 2.034 ± 0.395
0.0ThrXaa: 0.0 ± 0.0
Val
2.967ValAla: 2.967 ± 0.568
0.932ValCys: 0.932 ± 0.257
3.39ValAsp: 3.39 ± 0.557
4.323ValGlu: 4.323 ± 0.646
3.051ValPhe: 3.051 ± 0.538
3.39ValGly: 3.39 ± 0.666
0.593ValHis: 0.593 ± 0.276
3.475ValIle: 3.475 ± 0.457
5.594ValLys: 5.594 ± 0.621
4.238ValLeu: 4.238 ± 0.54
1.695ValMet: 1.695 ± 0.447
2.967ValAsn: 2.967 ± 0.575
1.61ValPro: 1.61 ± 0.357
1.61ValGln: 1.61 ± 0.398
2.967ValArg: 2.967 ± 0.448
5.17ValSer: 5.17 ± 0.712
4.747ValThr: 4.747 ± 0.553
3.56ValVal: 3.56 ± 0.498
2.119ValTrp: 2.119 ± 1.08
2.373ValTyr: 2.373 ± 0.543
0.0ValXaa: 0.0 ± 0.0
Trp
0.509TrpAla: 0.509 ± 0.197
0.17TrpCys: 0.17 ± 0.133
0.763TrpAsp: 0.763 ± 0.346
1.102TrpGlu: 1.102 ± 0.218
0.848TrpPhe: 0.848 ± 0.282
1.102TrpGly: 1.102 ± 0.276
0.424TrpHis: 0.424 ± 0.187
1.017TrpIle: 1.017 ± 0.446
1.017TrpLys: 1.017 ± 0.274
1.78TrpLeu: 1.78 ± 0.341
0.085TrpMet: 0.085 ± 0.087
1.78TrpAsn: 1.78 ± 1.134
0.0TrpPro: 0.0 ± 0.0
0.678TrpGln: 0.678 ± 0.241
0.339TrpArg: 0.339 ± 0.152
0.763TrpSer: 0.763 ± 0.231
0.424TrpThr: 0.424 ± 0.175
0.932TrpVal: 0.932 ± 0.249
0.17TrpTrp: 0.17 ± 0.112
0.678TrpTyr: 0.678 ± 0.23
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.526TyrAla: 1.526 ± 0.36
0.593TyrCys: 0.593 ± 0.256
3.051TyrAsp: 3.051 ± 0.707
2.712TyrGlu: 2.712 ± 0.426
1.526TyrPhe: 1.526 ± 0.288
2.289TyrGly: 2.289 ± 0.433
0.424TyrHis: 0.424 ± 0.2
2.797TyrIle: 2.797 ± 0.449
3.221TyrLys: 3.221 ± 0.416
2.967TyrLeu: 2.967 ± 0.504
0.678TyrMet: 0.678 ± 0.227
1.102TyrAsn: 1.102 ± 0.317
1.187TyrPro: 1.187 ± 0.319
1.271TyrGln: 1.271 ± 0.274
2.458TyrArg: 2.458 ± 0.447
1.78TyrSer: 1.78 ± 0.365
1.865TyrThr: 1.865 ± 0.362
3.221TyrVal: 3.221 ± 0.481
0.593TyrTrp: 0.593 ± 0.218
1.78TyrTyr: 1.78 ± 0.364
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 68 proteins (11799 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski