Providencia phage vB_PreS_PR1
Average proteome isoelectric point is 5.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 157 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1S6KV52|A0A1S6KV52_9CAUD Uncharacterized protein OS=Providencia phage vB_PreS_PR1 OX=1931407 GN=PR1_106 PE=4 SV=1
MM1 pKa = 7.35 SGKK4 pKa = 9.68 EE5 pKa = 4.09 AIPEE9 pKa = 3.96 YY10 pKa = 10.48 FDD12 pKa = 5.16 IVIQEE17 pKa = 4.19 LATEE21 pKa = 4.3 YY22 pKa = 11.06 GWDD25 pKa = 3.6 EE26 pKa = 4.45 QEE28 pKa = 3.93 TTVYY32 pKa = 11.05 DD33 pKa = 4.05 FVEE36 pKa = 4.23 SLDD39 pKa = 3.55 NVVVYY44 pKa = 10.36 KK45 pKa = 10.74 LRR47 pKa = 11.84 EE48 pKa = 3.93 PLAVSMLMPSAVEE61 pKa = 3.55 IARR64 pKa = 11.84 NNNYY68 pKa = 8.78 TPEE71 pKa = 4.07 AVTFFLQLYY80 pKa = 7.87 TLLKK84 pKa = 9.48 VHH86 pKa = 6.62 AVSDD90 pKa = 4.02 NEE92 pKa = 4.45 VVFSSSYY99 pKa = 10.96 KK100 pKa = 9.12 DD101 pKa = 3.24 TSVSLTAYY109 pKa = 8.69 MVYY112 pKa = 10.91 DD113 pKa = 4.07 LVDD116 pKa = 3.28 SGFNNN121 pKa = 3.55
Molecular weight: 13.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.844
IPC2_protein 4.024
IPC_protein 3.935
Toseland 3.757
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.062
Wikipedia 3.808
Rodwell 3.77
Grimsley 3.668
Solomon 3.884
Lehninger 3.846
Nozaki 4.024
DTASelect 4.177
Thurlkill 3.795
EMBOSS 3.821
Sillero 4.05
Patrickios 0.604
IPC_peptide 3.897
IPC2_peptide 4.037
IPC2.peptide.svr19 3.944
Protein with the highest isoelectric point:
>tr|A0A1S6KV61|A0A1S6KV61_9CAUD Uncharacterized protein OS=Providencia phage vB_PreS_PR1 OX=1931407 GN=PR1_72 PE=4 SV=1
MM1 pKa = 7.72 LLLIIAIIWCVTGFYY16 pKa = 10.65 LAFTAIRR23 pKa = 11.84 SMKK26 pKa = 9.89 AISVNPHH33 pKa = 4.93 LRR35 pKa = 11.84 KK36 pKa = 10.18 LGVKK40 pKa = 9.69 SKK42 pKa = 10.59 SHH44 pKa = 6.89 LLPTPVTFVIVSALLSPFGVWADD67 pKa = 3.55 SVARR71 pKa = 11.84 DD72 pKa = 3.66 IVRR75 pKa = 11.84 SLNNGNN81 pKa = 3.5
Molecular weight: 8.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.312
IPC2_protein 9.736
IPC_protein 10.248
Toseland 10.818
ProMoST 10.394
Dawson 10.891
Bjellqvist 10.54
Wikipedia 11.052
Rodwell 11.286
Grimsley 10.921
Solomon 10.994
Lehninger 10.979
Nozaki 10.804
DTASelect 10.54
Thurlkill 10.804
EMBOSS 11.213
Sillero 10.818
Patrickios 11.096
IPC_peptide 11.008
IPC2_peptide 9.472
IPC2.peptide.svr19 8.572
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
157
0
157
31244
30
1237
199.0
22.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.521 ± 0.3
0.928 ± 0.089
6.289 ± 0.16
7.125 ± 0.235
4.135 ± 0.125
6.248 ± 0.215
1.892 ± 0.117
6.526 ± 0.187
6.686 ± 0.175
8.853 ± 0.234
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.769 ± 0.121
4.894 ± 0.17
3.626 ± 0.167
3.489 ± 0.142
4.522 ± 0.154
6.763 ± 0.206
6.04 ± 0.252
6.744 ± 0.188
1.245 ± 0.079
3.706 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here