Phocoenobacter uteri

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Phocoenobacter

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1947 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A379C943|A0A379C943_9PAST TPR repeat-containing protein NMB0313 OS=Phocoenobacter uteri OX=146806 GN=NCTC12872_00117 PE=3 SV=1
MM1 pKa = 7.33AAVGLFYY8 pKa = 10.98GSDD11 pKa = 3.34TGNTEE16 pKa = 4.11AVSKK20 pKa = 9.56MIQDD24 pKa = 3.38QLGANLIDD32 pKa = 3.36VHH34 pKa = 7.83DD35 pKa = 4.05IAKK38 pKa = 8.42STKK41 pKa = 9.64EE42 pKa = 4.18DD43 pKa = 3.05IEE45 pKa = 5.79AYY47 pKa = 10.25DD48 pKa = 3.89FLLIGIPTWYY58 pKa = 10.13YY59 pKa = 11.37GEE61 pKa = 4.66SQADD65 pKa = 3.34WDD67 pKa = 4.5DD68 pKa = 4.67FMPTLQEE75 pKa = 3.35IDD77 pKa = 3.64FTGKK81 pKa = 10.02IIGIFGCGDD90 pKa = 3.22QEE92 pKa = 5.04DD93 pKa = 4.3YY94 pKa = 11.79AEE96 pKa = 4.32YY97 pKa = 10.5FCDD100 pKa = 4.05AMGTVRR106 pKa = 11.84DD107 pKa = 3.92VVEE110 pKa = 4.02QNGGIIVGHH119 pKa = 7.23WSTEE123 pKa = 3.98GYY125 pKa = 10.18SFEE128 pKa = 4.18VSQALVDD135 pKa = 3.81EE136 pKa = 4.45NTFVGLCIDD145 pKa = 4.19EE146 pKa = 4.82DD147 pKa = 3.9RR148 pKa = 11.84QPEE151 pKa = 4.27LTEE154 pKa = 3.55QRR156 pKa = 11.84VQKK159 pKa = 9.92WCEE162 pKa = 3.62QLKK165 pKa = 11.26DD166 pKa = 4.1EE167 pKa = 4.72MCLSALAA174 pKa = 4.41

Molecular weight: 19.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A379CCX1|A0A379CCX1_9PAST Outer membrane protein P4 OS=Phocoenobacter uteri OX=146806 GN=hel PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LKK11 pKa = 10.41RR12 pKa = 11.84ARR14 pKa = 11.84SHH16 pKa = 6.24GFRR19 pKa = 11.84TRR21 pKa = 11.84MATKK25 pKa = 10.32NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.15GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1947

0

1947

633974

31

4055

325.6

36.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.537 ± 0.057

0.996 ± 0.023

5.276 ± 0.064

6.425 ± 0.063

4.473 ± 0.057

6.616 ± 0.067

1.925 ± 0.025

7.386 ± 0.051

7.123 ± 0.069

10.066 ± 0.094

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.377 ± 0.038

5.281 ± 0.076

3.484 ± 0.035

4.613 ± 0.054

3.945 ± 0.052

6.067 ± 0.042

5.498 ± 0.065

6.609 ± 0.056

1.071 ± 0.022

3.231 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski