Streptococcus satellite phage Javan434

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZMV7|A0A4D5ZMV7_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan434 OX=2558702 GN=JavanS434_0012 PE=4 SV=1
MM1 pKa = 6.88TLKK4 pKa = 10.04TFSDD8 pKa = 3.49KK9 pKa = 11.46AKK11 pKa = 9.43TFTFTYY17 pKa = 8.54YY18 pKa = 10.75FCDD21 pKa = 3.18QATAQVAGHH30 pKa = 6.95ALLGYY35 pKa = 7.54MTGTYY40 pKa = 8.19CQPVISLTYY49 pKa = 9.28KK50 pKa = 10.96DD51 pKa = 4.19KK52 pKa = 9.97GTLVAEE58 pKa = 4.26YY59 pKa = 11.19VEE61 pKa = 4.12DD62 pKa = 3.62HH63 pKa = 6.67KK64 pKa = 11.65LNKK67 pKa = 8.14TFKK70 pKa = 10.29RR71 pKa = 11.84ICDD74 pKa = 3.6SFKK77 pKa = 10.84DD78 pKa = 3.63YY79 pKa = 11.11HH80 pKa = 6.33KK81 pKa = 10.87QPEE84 pKa = 3.9EE85 pKa = 4.19AEE87 pKa = 4.19AFEE90 pKa = 4.21EE91 pKa = 4.79RR92 pKa = 11.84YY93 pKa = 9.82KK94 pKa = 10.7RR95 pKa = 11.84EE96 pKa = 3.48RR97 pKa = 11.84VLQLKK102 pKa = 9.9EE103 pKa = 4.29SEE105 pKa = 4.47DD106 pKa = 3.97FEE108 pKa = 4.97SLLEE112 pKa = 4.08KK113 pKa = 9.84VTDD116 pKa = 3.93YY117 pKa = 11.2EE118 pKa = 5.77LEE120 pKa = 4.13LLDD123 pKa = 3.9YY124 pKa = 11.08ADD126 pKa = 5.36RR127 pKa = 11.84LLSDD131 pKa = 3.85TPIPMDD137 pKa = 3.36SMTAFGTLEE146 pKa = 3.95KK147 pKa = 10.88LGDD150 pKa = 3.75EE151 pKa = 5.13SISLLQKK158 pKa = 10.52LDD160 pKa = 3.32VEE162 pKa = 4.81GEE164 pKa = 4.33YY165 pKa = 10.49KK166 pKa = 10.72GLAGYY171 pKa = 7.7TEE173 pKa = 4.17HH174 pKa = 7.28LKK176 pKa = 10.96

Molecular weight: 20.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZSZ4|A0A4D5ZSZ4_9VIRU Glycerate kinase OS=Streptococcus satellite phage Javan434 OX=2558702 GN=JavanS434_0011 PE=4 SV=1
MM1 pKa = 7.64FDD3 pKa = 4.35GFPEE7 pKa = 4.13RR8 pKa = 11.84PRR10 pKa = 11.84YY11 pKa = 8.12MKK13 pKa = 9.81RR14 pKa = 11.84DD15 pKa = 3.77RR16 pKa = 11.84YY17 pKa = 9.2HH18 pKa = 5.94KK19 pKa = 10.09HH20 pKa = 4.58YY21 pKa = 10.97KK22 pKa = 10.24KK23 pKa = 10.13FLKK26 pKa = 10.6YY27 pKa = 9.68IEE29 pKa = 4.58KK30 pKa = 10.6GDD32 pKa = 3.91RR33 pKa = 11.84FWLNGSRR40 pKa = 11.84LL41 pKa = 3.57

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18

0

18

3102

41

484

172.3

19.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.028 ± 0.319

0.322 ± 0.119

5.642 ± 0.309

8.285 ± 0.573

4.417 ± 0.311

5.029 ± 0.481

1.902 ± 0.242

6.705 ± 0.6

9.768 ± 0.514

9.381 ± 0.542

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.676 ± 0.324

4.642 ± 0.629

3.127 ± 0.516

4.739 ± 0.321

5.448 ± 0.414

5.609 ± 0.317

6.286 ± 0.451

4.932 ± 0.482

0.709 ± 0.125

4.352 ± 0.293
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski