Capybara microvirus Cap1_SP_95
Average proteome isoelectric point is 5.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVN6|A0A4V1FVN6_9VIRU Nonstructural protein OS=Capybara microvirus Cap1_SP_95 OX=2584801 PE=4 SV=1
MM1 pKa = 7.01 KK2 pKa = 9.87 TYY4 pKa = 9.82 MDD6 pKa = 5.26 SIDD9 pKa = 4.75 SGSQYY14 pKa = 11.13 QFGDD18 pKa = 3.31 KK19 pKa = 10.38 LLQAEE24 pKa = 4.41 NPKK27 pKa = 10.6 SAFDD31 pKa = 4.06 LSHH34 pKa = 6.93 LNSLTINNCGSLIPIALWEE53 pKa = 4.37 TVPSDD58 pKa = 3.27 TFEE61 pKa = 4.58 ISVTSLVRR69 pKa = 11.84 VLPQVVPLYY78 pKa = 10.43 SRR80 pKa = 11.84 QRR82 pKa = 11.84 LYY84 pKa = 11.15 VYY86 pKa = 10.69 AFYY89 pKa = 11.29 SRR91 pKa = 11.84 ACDD94 pKa = 3.16 LWKK97 pKa = 10.58 NAHH100 pKa = 6.58 AYY102 pKa = 8.58 YY103 pKa = 10.71 KK104 pKa = 10.33 KK105 pKa = 10.65 GYY107 pKa = 9.61 DD108 pKa = 3.29 GNYY111 pKa = 9.94 VINKK115 pKa = 8.75 PVLTEE120 pKa = 4.18 NNMSPGLWDD129 pKa = 3.64 SKK131 pKa = 9.84 ITADD135 pKa = 5.05 SLGDD139 pKa = 3.8 YY140 pKa = 10.73 LGLPQGFTYY149 pKa = 10.65 KK150 pKa = 10.16 EE151 pKa = 3.98 LSEE154 pKa = 4.14 TAPINALPFFMYY166 pKa = 10.38 EE167 pKa = 4.28 KK168 pKa = 10.06 IFQAFFIEE176 pKa = 4.75 KK177 pKa = 9.64 NLPYY181 pKa = 10.66 YY182 pKa = 10.21 RR183 pKa = 11.84 DD184 pKa = 3.28 NRR186 pKa = 11.84 AWLPHH191 pKa = 6.97 DD192 pKa = 3.74 EE193 pKa = 4.35 ADD195 pKa = 4.18 LRR197 pKa = 11.84 FGATDD202 pKa = 3.46 NEE204 pKa = 4.74 IISNSYY210 pKa = 9.39 LAEE213 pKa = 4.28 GQTGITLGSLEE224 pKa = 3.99 YY225 pKa = 10.48 RR226 pKa = 11.84 NYY228 pKa = 9.99 PLDD231 pKa = 3.88 YY232 pKa = 8.81 FTSALPSPTRR242 pKa = 11.84 GTAPTLPFTASLSEE256 pKa = 4.09 NPLVRR261 pKa = 11.84 FDD263 pKa = 3.94 TSPTPSSVYY272 pKa = 10.47 DD273 pKa = 3.95 FNSGPSASGGIRR285 pKa = 11.84 RR286 pKa = 11.84 AGVGYY291 pKa = 9.36 FYY293 pKa = 11.39 ANFPIEE299 pKa = 4.46 SNSNIPLGGNGSDD312 pKa = 3.67 IIIPAGSGSSIAYY325 pKa = 8.01 RR326 pKa = 11.84 HH327 pKa = 6.45 AYY329 pKa = 10.03 VDD331 pKa = 3.41 MSMSEE336 pKa = 4.1 FLSNITLDD344 pKa = 3.34 KK345 pKa = 10.73 FRR347 pKa = 11.84 EE348 pKa = 4.01 LSITTEE354 pKa = 3.9 EE355 pKa = 4.37 VEE357 pKa = 4.61 RR358 pKa = 11.84 MARR361 pKa = 11.84 TDD363 pKa = 2.98 GSYY366 pKa = 11.45 VDD368 pKa = 5.06 FGTTFFGIVCKK379 pKa = 10.52 NAFDD383 pKa = 5.01 FKK385 pKa = 10.02 PTLIGATYY393 pKa = 10.5 QSMNFSEE400 pKa = 4.75 VLQTTPTSTSPLGAYY415 pKa = 9.17 AGHH418 pKa = 7.12 GISVPTNNGYY428 pKa = 9.98 LGKK431 pKa = 9.85 IVCDD435 pKa = 3.96 DD436 pKa = 3.58 YY437 pKa = 11.67 GYY439 pKa = 10.95 IMIVATIMPDD449 pKa = 3.07 VYY451 pKa = 11.02 YY452 pKa = 10.64 HH453 pKa = 6.57 QGLSKK458 pKa = 10.78 LWTKK462 pKa = 10.55 SLQSEE467 pKa = 4.24 EE468 pKa = 4.45 FLPKK472 pKa = 9.09 RR473 pKa = 11.84 AKK475 pKa = 9.64 MGLIPVLNRR484 pKa = 11.84 EE485 pKa = 4.45 LFVSGDD491 pKa = 3.49 VVTDD495 pKa = 3.58 RR496 pKa = 11.84 DD497 pKa = 3.47 LFAYY501 pKa = 9.07 QNPFDD506 pKa = 4.22 EE507 pKa = 4.58 MRR509 pKa = 11.84 YY510 pKa = 8.1 MPNEE514 pKa = 3.65 IHH516 pKa = 7.05 GKK518 pKa = 9.38 IADD521 pKa = 3.92 SEE523 pKa = 4.32 NLSFFPYY530 pKa = 8.31 TQARR534 pKa = 11.84 HH535 pKa = 4.56 FTSTPTYY542 pKa = 9.44 SKK544 pKa = 11.04 EE545 pKa = 3.71 FFEE548 pKa = 5.2 ARR550 pKa = 11.84 DD551 pKa = 3.46 VRR553 pKa = 11.84 KK554 pKa = 10.28 DD555 pKa = 3.51 YY556 pKa = 11.19 LAGGTAEE563 pKa = 4.66 DD564 pKa = 4.8 AYY566 pKa = 11.17 SAQFKK571 pKa = 10.57 FDD573 pKa = 3.18 IRR575 pKa = 11.84 AVRR578 pKa = 11.84 PLSYY582 pKa = 10.25 IPVPAVVVV590 pKa = 3.66
Molecular weight: 65.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.812
IPC2_protein 4.991
IPC_protein 4.94
Toseland 4.838
ProMoST 5.08
Dawson 4.927
Bjellqvist 5.054
Wikipedia 4.8
Rodwell 4.825
Grimsley 4.762
Solomon 4.927
Lehninger 4.876
Nozaki 5.041
DTASelect 5.219
Thurlkill 4.838
EMBOSS 4.838
Sillero 5.105
Patrickios 3.21
IPC_peptide 4.927
IPC2_peptide 5.092
IPC2.peptide.svr19 5.062
Protein with the highest isoelectric point:
>tr|A0A4P8W7P3|A0A4P8W7P3_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_95 OX=2584801 PE=3 SV=1
MM1 pKa = 7.66 VFEE4 pKa = 4.53 IAFMACVYY12 pKa = 9.87 PLRR15 pKa = 11.84 LRR17 pKa = 11.84 NVRR20 pKa = 11.84 SGLPLDD26 pKa = 4.23 VACGQCMNCRR36 pKa = 11.84 VQKK39 pKa = 9.81 TMSLSWLANMEE50 pKa = 4.16 LYY52 pKa = 9.57 TRR54 pKa = 11.84 YY55 pKa = 10.1 RR56 pKa = 11.84 KK57 pKa = 9.73 GQSASFVTLTYY68 pKa = 10.88 DD69 pKa = 3.8 DD70 pKa = 4.41 SFLPRR75 pKa = 11.84 VVCSDD80 pKa = 3.28 GAVRR84 pKa = 11.84 PTLRR88 pKa = 11.84 KK89 pKa = 9.63 VDD91 pKa = 3.62 LQRR94 pKa = 11.84 FFKK97 pKa = 10.48 RR98 pKa = 11.84 VRR100 pKa = 11.84 KK101 pKa = 9.38 NLSDD105 pKa = 4.89 SGIKK109 pKa = 10.49 DD110 pKa = 2.98 NWSYY114 pKa = 10.68 IACGEE119 pKa = 4.4 HH120 pKa = 6.22 GDD122 pKa = 3.86 KK123 pKa = 10.77 FNRR126 pKa = 11.84 PHH128 pKa = 5.36 YY129 pKa = 10.39 HH130 pKa = 6.0 FVALGLSDD138 pKa = 5.12 SICDD142 pKa = 3.61 YY143 pKa = 10.48 SVRR146 pKa = 11.84 RR147 pKa = 11.84 AWRR150 pKa = 11.84 FGLTDD155 pKa = 3.25 TGVLRR160 pKa = 11.84 QGGLNYY166 pKa = 9.64 VCKK169 pKa = 10.68 YY170 pKa = 5.33 MTKK173 pKa = 10.68 APTGRR178 pKa = 11.84 LAKK181 pKa = 10.32 EE182 pKa = 3.98 LFTDD186 pKa = 4.69 LGLEE190 pKa = 4.16 KK191 pKa = 10.54 PFVQHH196 pKa = 5.68 SQNLGMQWILDD207 pKa = 3.79 NYY209 pKa = 10.38 RR210 pKa = 11.84 SLVEE214 pKa = 4.75 NNYY217 pKa = 10.38 CYY219 pKa = 10.88 YY220 pKa = 11.09 DD221 pKa = 3.67 NGKK224 pKa = 10.28 LIPLPSALRR233 pKa = 11.84 RR234 pKa = 11.84 RR235 pKa = 11.84 LDD237 pKa = 2.82 IYY239 pKa = 10.63 KK240 pKa = 10.36 QYY242 pKa = 11.2 DD243 pKa = 3.27 DD244 pKa = 4.78 SIQIKK249 pKa = 9.78 RR250 pKa = 11.84 LKK252 pKa = 10.8 DD253 pKa = 3.2 SARR256 pKa = 11.84 QNGFGDD262 pKa = 3.11 SWQDD266 pKa = 3.24 YY267 pKa = 10.6 ARR269 pKa = 11.84 IHH271 pKa = 6.22 AFNVEE276 pKa = 3.86 KK277 pKa = 10.39 MVIDD281 pKa = 3.96 SSRR284 pKa = 11.84 SSGVPQFDD292 pKa = 4.31 CDD294 pKa = 3.66 YY295 pKa = 11.06 SSADD299 pKa = 4.6 KK300 pKa = 10.91 IPIYY304 pKa = 10.6 NNHH307 pKa = 5.79 NVNVANVDD315 pKa = 3.85 YY316 pKa = 9.56 LTTEE320 pKa = 4.2 CLDD323 pKa = 3.61 PVPFF327 pKa = 4.88
Molecular weight: 37.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.987
IPC2_protein 8.083
IPC_protein 7.936
Toseland 7.556
ProMoST 8.448
Dawson 8.624
Bjellqvist 8.946
Wikipedia 8.58
Rodwell 8.653
Grimsley 7.512
Solomon 8.712
Lehninger 8.697
Nozaki 9.063
DTASelect 8.668
Thurlkill 8.712
EMBOSS 8.799
Sillero 9.019
Patrickios 4.024
IPC_peptide 8.697
IPC2_peptide 8.009
IPC2.peptide.svr19 8.153
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1524
87
590
254.0
28.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.283 ± 0.947
1.378 ± 0.63
6.759 ± 0.576
5.249 ± 0.822
5.446 ± 0.671
5.709 ± 0.51
1.575 ± 0.315
4.724 ± 0.652
5.249 ± 0.795
8.202 ± 0.47
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.297 ± 0.17
5.709 ± 0.484
4.921 ± 0.722
3.215 ± 0.681
4.987 ± 0.872
9.777 ± 0.819
4.856 ± 0.888
5.906 ± 0.751
1.05 ± 0.241
5.709 ± 0.893
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here