Puniceicoccales bacterium CK1056

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Opitutae; Puniceicoccales; unclassified Puniceicoccales

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2818 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B2LZZ1|A0A6B2LZZ1_9BACT Uncharacterized protein OS=Puniceicoccales bacterium CK1056 OX=2706888 GN=G0Q06_04600 PE=4 SV=1
MM1 pKa = 7.63KK2 pKa = 10.06PRR4 pKa = 11.84TKK6 pKa = 10.39RR7 pKa = 11.84SLVPLMAAACLFTIVVMVPRR27 pKa = 11.84AEE29 pKa = 4.51AIIAGSNWDD38 pKa = 2.77IHH40 pKa = 5.65MDD42 pKa = 3.29IEE44 pKa = 4.53GVVANDD50 pKa = 2.98YY51 pKa = 10.62HH52 pKa = 8.08IEE54 pKa = 3.97GVIYY58 pKa = 10.56SGIPGEE64 pKa = 4.16NWSYY68 pKa = 11.18PPTLVDD74 pKa = 5.05HH75 pKa = 7.46IDD77 pKa = 3.54GNFPNFSYY85 pKa = 11.62SMVPDD90 pKa = 4.8LSDD93 pKa = 4.3PDD95 pKa = 3.24QFAYY99 pKa = 9.71IFQADD104 pKa = 3.57WTGADD109 pKa = 3.67YY110 pKa = 10.4EE111 pKa = 4.59FCQDD115 pKa = 3.09LHH117 pKa = 7.97LGLFFDD123 pKa = 5.09LEE125 pKa = 4.49CHH127 pKa = 6.53NLMIDD132 pKa = 4.91LKK134 pKa = 10.97GWWTKK139 pKa = 10.9DD140 pKa = 3.28GEE142 pKa = 4.56PVGMTLLPGFEE153 pKa = 4.83AEE155 pKa = 5.19DD156 pKa = 3.75EE157 pKa = 4.56FGPTTPHH164 pKa = 5.89LRR166 pKa = 11.84LRR168 pKa = 11.84NDD170 pKa = 3.13SQIPGEE176 pKa = 4.16IVALRR181 pKa = 11.84LTGLSPEE188 pKa = 4.14EE189 pKa = 3.99VEE191 pKa = 4.98GLFGTAQDD199 pKa = 4.55MFPEE203 pKa = 4.73LAPGGLLDD211 pKa = 4.6PEE213 pKa = 4.85NPQSPVPWSEE223 pKa = 3.83GKK225 pKa = 10.78EE226 pKa = 3.88MDD228 pKa = 3.94PTGQLIPMGPKK239 pKa = 10.22DD240 pKa = 4.24FPAEE244 pKa = 3.98SFFDD248 pKa = 3.32VFYY251 pKa = 10.49EE252 pKa = 4.18IEE254 pKa = 4.38IDD256 pKa = 3.84TEE258 pKa = 4.34PGDD261 pKa = 3.92IVADD265 pKa = 3.75PPLAMEE271 pKa = 5.28AGNILVAAEE280 pKa = 3.84LVRR283 pKa = 11.84YY284 pKa = 9.48INEE287 pKa = 4.15SGVEE291 pKa = 3.99EE292 pKa = 5.28FNWTWEE298 pKa = 3.94LHH300 pKa = 5.51EE301 pKa = 4.35SHH303 pKa = 6.98GIDD306 pKa = 5.05FGDD309 pKa = 5.28APDD312 pKa = 3.74QSYY315 pKa = 7.78QTLLASDD322 pKa = 4.78GARR325 pKa = 11.84HH326 pKa = 6.19IAQGVYY332 pKa = 9.96LGQVRR337 pKa = 11.84DD338 pKa = 4.08TEE340 pKa = 5.2LDD342 pKa = 3.67GQPTALADD350 pKa = 4.05GDD352 pKa = 4.57DD353 pKa = 4.2NNPAGGPDD361 pKa = 4.04DD362 pKa = 4.21EE363 pKa = 7.32DD364 pKa = 4.06GVTFTGSFVPGSAVGLNVQVNGTGFLNIWADD395 pKa = 3.51WNRR398 pKa = 11.84DD399 pKa = 3.56GMWDD403 pKa = 3.41PSEE406 pKa = 4.32QVLTPDD412 pKa = 3.69IPVSTGTFVGAIVVPPAPPTGFGPTYY438 pKa = 9.26MRR440 pKa = 11.84FRR442 pKa = 11.84VSSQPGLQWFGLAVDD457 pKa = 5.16GEE459 pKa = 4.6VEE461 pKa = 4.55DD462 pKa = 4.05YY463 pKa = 10.93RR464 pKa = 11.84IDD466 pKa = 3.78ILDD469 pKa = 4.2SNDD472 pKa = 4.7DD473 pKa = 3.75VYY475 pKa = 11.88DD476 pKa = 3.53WGDD479 pKa = 3.67APDD482 pKa = 3.92AAAGPAYY489 pKa = 7.17PTWASSNGARR499 pKa = 11.84HH500 pKa = 6.01LLSSQLWLGGGVDD513 pKa = 4.81PEE515 pKa = 4.73PDD517 pKa = 3.69GQPTIPADD525 pKa = 3.77GDD527 pKa = 4.31DD528 pKa = 3.63ISGFPDD534 pKa = 4.42DD535 pKa = 4.69EE536 pKa = 6.76DD537 pKa = 4.8GVALPISLMQGITATVNVFASGSGLLNAWVDD568 pKa = 4.24FDD570 pKa = 5.58QNSSWLDD577 pKa = 3.31AFEE580 pKa = 4.44HH581 pKa = 6.39VIVDD585 pKa = 3.78APVVGGPNSLSFLVPAVALPGNTYY609 pKa = 9.27MRR611 pKa = 11.84FRR613 pKa = 11.84LSTQAGLTPSGPAPDD628 pKa = 5.07GEE630 pKa = 4.48VEE632 pKa = 4.62DD633 pKa = 4.03YY634 pKa = 11.16LVLIEE639 pKa = 4.58EE640 pKa = 4.58SAAQGFDD647 pKa = 2.83WGDD650 pKa = 3.4APDD653 pKa = 4.55FPQVPGYY660 pKa = 7.03PTLSIHH666 pKa = 6.64NGAHH670 pKa = 6.15HH671 pKa = 6.7FLGGVYY677 pKa = 10.13LGNVVDD683 pKa = 5.22PEE685 pKa = 4.45PDD687 pKa = 3.48GQPTIPADD695 pKa = 4.11GDD697 pKa = 3.78DD698 pKa = 4.49LAALPDD704 pKa = 3.76EE705 pKa = 5.4DD706 pKa = 4.81GVTFLMHH713 pKa = 6.5LVKK716 pKa = 10.85GKK718 pKa = 8.89TSQIQVWASAVGFLNAWMDD737 pKa = 3.92YY738 pKa = 10.1NQNTSWADD746 pKa = 3.29PGEE749 pKa = 4.21HH750 pKa = 6.07VFIDD754 pKa = 3.95TPLVAGPNNLTLTLPASAVFGNTYY778 pKa = 9.8FRR780 pKa = 11.84FRR782 pKa = 11.84FSSAPGLSFDD792 pKa = 4.62GFASDD797 pKa = 6.08GEE799 pKa = 4.45VEE801 pKa = 4.26DD802 pKa = 4.43YY803 pKa = 10.69RR804 pKa = 11.84VEE806 pKa = 4.17VCEE809 pKa = 5.02LADD812 pKa = 3.76TVITTTASNDD822 pKa = 3.15VVLNWTPVTGATSYY836 pKa = 11.29AIYY839 pKa = 10.18SANHH843 pKa = 6.33LGSFYY848 pKa = 10.39LWNYY852 pKa = 6.8MGPAAGPPWTHH863 pKa = 6.59TAPGWMPLSFYY874 pKa = 11.11YY875 pKa = 10.65VVAEE879 pKa = 4.12PP880 pKa = 4.2

Molecular weight: 94.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B2M3E2|A0A6B2M3E2_9BACT Uncharacterized protein OS=Puniceicoccales bacterium CK1056 OX=2706888 GN=G0Q06_13515 PE=4 SV=1
MM1 pKa = 7.6GNLRR5 pKa = 11.84KK6 pKa = 9.62KK7 pKa = 10.1RR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.37ISQHH15 pKa = 4.33KK16 pKa = 8.47RR17 pKa = 11.84KK18 pKa = 9.69KK19 pKa = 8.65RR20 pKa = 11.84SRR22 pKa = 11.84SNRR25 pKa = 11.84HH26 pKa = 5.79KK27 pKa = 10.72KK28 pKa = 8.31RR29 pKa = 11.84TWGG32 pKa = 3.39

Molecular weight: 4.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2818

0

2818

1091845

24

14125

387.5

42.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.418 ± 0.038

0.921 ± 0.022

5.906 ± 0.075

6.733 ± 0.057

4.488 ± 0.033

8.225 ± 0.064

2.005 ± 0.028

5.895 ± 0.037

4.247 ± 0.056

10.06 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.166 ± 0.03

3.726 ± 0.045

4.967 ± 0.039

3.299 ± 0.03

5.555 ± 0.064

6.681 ± 0.045

5.465 ± 0.067

6.762 ± 0.035

1.578 ± 0.026

2.902 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski