Gordonia Phage Barsten

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Vividuovirus; unclassified Vividuovirus

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 88 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G3V9I8|A0A7G3V9I8_9CAUD Uncharacterized protein OS=Gordonia Phage Barsten OX=2743907 GN=5 PE=4 SV=1
MM1 pKa = 7.29SRR3 pKa = 11.84QARR6 pKa = 11.84VRR8 pKa = 11.84YY9 pKa = 8.77LAGASLVIDD18 pKa = 3.9VPDD21 pKa = 4.44DD22 pKa = 3.96VDD24 pKa = 3.52VSTPAEE30 pKa = 3.79LLAWAEE36 pKa = 4.35EE37 pKa = 4.16NADD40 pKa = 3.81TYY42 pKa = 10.88VTLCHH47 pKa = 5.64QCAHH51 pKa = 7.07EE52 pKa = 4.23ISLGDD57 pKa = 3.9FEE59 pKa = 5.36AHH61 pKa = 5.53TVVDD65 pKa = 4.21DD66 pKa = 4.36AGEE69 pKa = 3.98EE70 pKa = 3.98HH71 pKa = 6.93AVADD75 pKa = 4.31IEE77 pKa = 4.72APQQ80 pKa = 3.38

Molecular weight: 8.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G3VBG4|A0A7G3VBG4_9CAUD Uncharacterized protein OS=Gordonia Phage Barsten OX=2743907 GN=79 PE=4 SV=1
MM1 pKa = 7.52RR2 pKa = 11.84RR3 pKa = 11.84LRR5 pKa = 11.84PRR7 pKa = 11.84PRR9 pKa = 11.84SAPHH13 pKa = 5.67RR14 pKa = 11.84RR15 pKa = 11.84AVRR18 pKa = 11.84RR19 pKa = 11.84PTLPMPPPPPRR30 pKa = 11.84NGDD33 pKa = 3.27PHH35 pKa = 5.39VTVILGIDD43 pKa = 3.57PSLTSTGLARR53 pKa = 11.84ITITPTAVSTTAPGVDD69 pKa = 3.75DD70 pKa = 3.59VTIQTTCVGEE80 pKa = 4.04PGGKK84 pKa = 9.76GATVEE89 pKa = 3.88QRR91 pKa = 11.84RR92 pKa = 11.84ARR94 pKa = 11.84IQRR97 pKa = 11.84ARR99 pKa = 11.84RR100 pKa = 11.84SILRR104 pKa = 11.84AAQGVDD110 pKa = 3.23LAVIEE115 pKa = 4.39VPFYY119 pKa = 11.08NRR121 pKa = 11.84QTTQVGLMDD130 pKa = 5.76RR131 pKa = 11.84SWLWGTIVDD140 pKa = 4.42GLHH143 pKa = 6.43AADD146 pKa = 3.62IPVVHH151 pKa = 6.39VAAKK155 pKa = 9.94QRR157 pKa = 11.84AKK159 pKa = 10.61FATDD163 pKa = 2.96NGNADD168 pKa = 3.55KK169 pKa = 11.15AQVAEE174 pKa = 4.63AIGRR178 pKa = 11.84LWSAVLVEE186 pKa = 4.49NGRR189 pKa = 11.84HH190 pKa = 4.41RR191 pKa = 11.84QLRR194 pKa = 11.84NDD196 pKa = 4.01DD197 pKa = 4.07EE198 pKa = 5.64YY199 pKa = 11.82DD200 pKa = 3.61ALVCATIGAVKK211 pKa = 8.02THH213 pKa = 6.25PRR215 pKa = 11.84SRR217 pKa = 11.84LPIRR221 pKa = 11.84VLEE224 pKa = 4.13HH225 pKa = 6.54HH226 pKa = 6.5LHH228 pKa = 5.51VVAGLDD234 pKa = 3.28WQAWDD239 pKa = 3.78TSEE242 pKa = 5.89QGITWW247 pKa = 3.73

Molecular weight: 27.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

88

0

88

19041

37

1607

216.4

23.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.352 ± 0.366

0.788 ± 0.108

7.473 ± 0.446

5.541 ± 0.379

2.474 ± 0.168

8.545 ± 0.444

2.143 ± 0.191

4.559 ± 0.255

2.516 ± 0.197

7.573 ± 0.26

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.09 ± 0.114

2.615 ± 0.158

6.271 ± 0.267

3.776 ± 0.199

7.368 ± 0.45

4.842 ± 0.252

6.859 ± 0.38

7.888 ± 0.267

2.137 ± 0.12

2.19 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski