Amino acid dipepetide frequency for Gordonia Phage Barsten

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.279AlaAla: 17.279 ± 1.173
0.683AlaCys: 0.683 ± 0.211
8.088AlaAsp: 8.088 ± 0.791
9.139AlaGlu: 9.139 ± 0.948
2.941AlaPhe: 2.941 ± 0.5
11.922AlaGly: 11.922 ± 0.876
2.101AlaHis: 2.101 ± 0.306
6.25AlaIle: 6.25 ± 1.019
4.149AlaLys: 4.149 ± 0.563
9.244AlaLeu: 9.244 ± 0.854
2.258AlaMet: 2.258 ± 0.358
2.994AlaAsn: 2.994 ± 0.54
6.723AlaPro: 6.723 ± 0.772
4.622AlaGln: 4.622 ± 0.446
8.246AlaArg: 8.246 ± 0.803
5.882AlaSer: 5.882 ± 0.698
8.088AlaThr: 8.088 ± 0.974
8.351AlaVal: 8.351 ± 0.658
2.521AlaTrp: 2.521 ± 0.383
2.048AlaTyr: 2.048 ± 0.334
0.0AlaXaa: 0.0 ± 0.0
Cys
1.366CysAla: 1.366 ± 0.349
0.0CysCys: 0.0 ± 0.0
0.473CysAsp: 0.473 ± 0.187
0.42CysGlu: 0.42 ± 0.137
0.105CysPhe: 0.105 ± 0.066
1.103CysGly: 1.103 ± 0.301
0.42CysHis: 0.42 ± 0.149
0.053CysIle: 0.053 ± 0.048
0.263CysLys: 0.263 ± 0.108
0.263CysLeu: 0.263 ± 0.103
0.158CysMet: 0.158 ± 0.089
0.053CysAsn: 0.053 ± 0.041
0.63CysPro: 0.63 ± 0.179
0.263CysGln: 0.263 ± 0.106
0.525CysArg: 0.525 ± 0.226
0.578CysSer: 0.578 ± 0.185
0.525CysThr: 0.525 ± 0.179
0.578CysVal: 0.578 ± 0.22
0.105CysTrp: 0.105 ± 0.082
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
8.403AspAla: 8.403 ± 0.831
0.21AspCys: 0.21 ± 0.102
7.721AspAsp: 7.721 ± 1.006
5.672AspGlu: 5.672 ± 0.898
1.261AspPhe: 1.261 ± 0.285
6.67AspGly: 6.67 ± 0.658
1.576AspHis: 1.576 ± 0.35
1.733AspIle: 1.733 ± 0.259
1.681AspLys: 1.681 ± 0.293
4.937AspLeu: 4.937 ± 0.558
1.681AspMet: 1.681 ± 0.27
2.311AspAsn: 2.311 ± 0.349
7.405AspPro: 7.405 ± 0.744
2.468AspGln: 2.468 ± 0.54
5.252AspArg: 5.252 ± 0.69
2.206AspSer: 2.206 ± 0.292
3.624AspThr: 3.624 ± 0.453
6.46AspVal: 6.46 ± 0.647
1.786AspTrp: 1.786 ± 0.324
1.681AspTyr: 1.681 ± 0.292
0.0AspXaa: 0.0 ± 0.0
Glu
5.41GluAla: 5.41 ± 0.496
0.63GluCys: 0.63 ± 0.184
3.204GluAsp: 3.204 ± 0.473
1.471GluGlu: 1.471 ± 0.286
2.206GluPhe: 2.206 ± 0.343
3.887GluGly: 3.887 ± 0.573
2.574GluHis: 2.574 ± 0.428
2.311GluIle: 2.311 ± 0.318
1.103GluLys: 1.103 ± 0.328
4.727GluLeu: 4.727 ± 0.742
1.05GluMet: 1.05 ± 0.239
1.366GluAsn: 1.366 ± 0.226
2.889GluPro: 2.889 ± 0.467
4.097GluGln: 4.097 ± 0.521
4.202GluArg: 4.202 ± 0.724
2.889GluSer: 2.889 ± 0.407
2.784GluThr: 2.784 ± 0.407
5.2GluVal: 5.2 ± 0.605
1.891GluTrp: 1.891 ± 0.332
1.523GluTyr: 1.523 ± 0.309
0.0GluXaa: 0.0 ± 0.0
Phe
3.204PheAla: 3.204 ± 0.43
0.368PheCys: 0.368 ± 0.137
3.046PheAsp: 3.046 ± 0.448
1.523PheGlu: 1.523 ± 0.338
0.525PhePhe: 0.525 ± 0.199
2.784PheGly: 2.784 ± 0.354
0.42PheHis: 0.42 ± 0.148
1.366PheIle: 1.366 ± 0.283
0.473PheLys: 0.473 ± 0.167
1.838PheLeu: 1.838 ± 0.315
0.473PheMet: 0.473 ± 0.172
0.683PheAsn: 0.683 ± 0.187
0.998PhePro: 0.998 ± 0.209
0.525PheGln: 0.525 ± 0.192
1.05PheArg: 1.05 ± 0.259
0.945PheSer: 0.945 ± 0.305
1.838PheThr: 1.838 ± 0.309
2.206PheVal: 2.206 ± 0.292
0.315PheTrp: 0.315 ± 0.117
0.158PheTyr: 0.158 ± 0.087
0.0PheXaa: 0.0 ± 0.0
Gly
9.296GlyAla: 9.296 ± 1.053
0.735GlyCys: 0.735 ± 0.253
6.408GlyAsp: 6.408 ± 0.601
4.727GlyGlu: 4.727 ± 0.587
2.101GlyPhe: 2.101 ± 0.349
7.563GlyGly: 7.563 ± 1.024
2.101GlyHis: 2.101 ± 0.329
4.202GlyIle: 4.202 ± 0.478
3.151GlyLys: 3.151 ± 0.445
5.987GlyLeu: 5.987 ± 0.821
2.101GlyMet: 2.101 ± 0.262
2.521GlyAsn: 2.521 ± 0.377
3.834GlyPro: 3.834 ± 0.51
3.571GlyGln: 3.571 ± 0.409
5.987GlyArg: 5.987 ± 0.616
5.357GlySer: 5.357 ± 0.65
5.357GlyThr: 5.357 ± 0.706
7.195GlyVal: 7.195 ± 0.662
1.366GlyTrp: 1.366 ± 0.232
1.891GlyTyr: 1.891 ± 0.344
0.0GlyXaa: 0.0 ± 0.0
His
2.363HisAla: 2.363 ± 0.347
0.053HisCys: 0.053 ± 0.044
1.996HisAsp: 1.996 ± 0.362
1.471HisGlu: 1.471 ± 0.302
0.368HisPhe: 0.368 ± 0.145
1.366HisGly: 1.366 ± 0.223
0.21HisHis: 0.21 ± 0.097
1.05HisIle: 1.05 ± 0.266
0.368HisLys: 0.368 ± 0.144
1.891HisLeu: 1.891 ± 0.328
0.473HisMet: 0.473 ± 0.187
0.473HisAsn: 0.473 ± 0.174
1.208HisPro: 1.208 ± 0.266
0.893HisGln: 0.893 ± 0.179
1.891HisArg: 1.891 ± 0.339
0.735HisSer: 0.735 ± 0.195
1.996HisThr: 1.996 ± 0.355
1.786HisVal: 1.786 ± 0.33
0.42HisTrp: 0.42 ± 0.144
0.42HisTyr: 0.42 ± 0.139
0.0HisXaa: 0.0 ± 0.0
Ile
6.197IleAla: 6.197 ± 0.57
0.315IleCys: 0.315 ± 0.143
4.359IleAsp: 4.359 ± 0.494
4.097IleGlu: 4.097 ± 0.403
0.42IlePhe: 0.42 ± 0.212
4.727IleGly: 4.727 ± 0.913
0.735IleHis: 0.735 ± 0.182
2.101IleIle: 2.101 ± 0.412
0.788IleLys: 0.788 ± 0.278
2.311IleLeu: 2.311 ± 0.378
0.84IleMet: 0.84 ± 0.191
0.84IleAsn: 0.84 ± 0.238
3.099IlePro: 3.099 ± 0.372
1.313IleGln: 1.313 ± 0.299
2.626IleArg: 2.626 ± 0.317
1.681IleSer: 1.681 ± 0.311
3.624IleThr: 3.624 ± 0.527
3.782IleVal: 3.782 ± 0.351
0.788IleTrp: 0.788 ± 0.216
0.945IleTyr: 0.945 ± 0.223
0.0IleXaa: 0.0 ± 0.0
Lys
3.151LysAla: 3.151 ± 0.536
0.105LysCys: 0.105 ± 0.072
0.945LysAsp: 0.945 ± 0.263
0.473LysGlu: 0.473 ± 0.146
0.893LysPhe: 0.893 ± 0.266
1.471LysGly: 1.471 ± 0.38
0.63LysHis: 0.63 ± 0.148
1.313LysIle: 1.313 ± 0.265
0.578LysLys: 0.578 ± 0.177
2.416LysLeu: 2.416 ± 0.339
0.525LysMet: 0.525 ± 0.188
0.788LysAsn: 0.788 ± 0.225
1.838LysPro: 1.838 ± 0.322
0.998LysGln: 0.998 ± 0.226
2.416LysArg: 2.416 ± 0.424
1.681LysSer: 1.681 ± 0.355
1.628LysThr: 1.628 ± 0.283
2.101LysVal: 2.101 ± 0.331
0.42LysTrp: 0.42 ± 0.15
0.788LysTyr: 0.788 ± 0.198
0.0LysXaa: 0.0 ± 0.0
Leu
9.139LeuAla: 9.139 ± 0.762
0.578LeuCys: 0.578 ± 0.183
5.357LeuAsp: 5.357 ± 0.598
2.468LeuGlu: 2.468 ± 0.362
2.101LeuPhe: 2.101 ± 0.368
6.88LeuGly: 6.88 ± 1.111
1.681LeuHis: 1.681 ± 0.312
3.204LeuIle: 3.204 ± 0.386
1.155LeuLys: 1.155 ± 0.231
5.935LeuLeu: 5.935 ± 0.544
1.261LeuMet: 1.261 ± 0.207
2.206LeuAsn: 2.206 ± 0.32
4.779LeuPro: 4.779 ± 0.444
3.046LeuGln: 3.046 ± 0.424
6.092LeuArg: 6.092 ± 0.483
3.676LeuSer: 3.676 ± 0.411
6.092LeuThr: 6.092 ± 0.547
6.303LeuVal: 6.303 ± 0.545
1.891LeuTrp: 1.891 ± 0.306
1.891LeuTyr: 1.891 ± 0.349
0.0LeuXaa: 0.0 ± 0.0
Met
2.153MetAla: 2.153 ± 0.294
0.105MetCys: 0.105 ± 0.078
0.893MetAsp: 0.893 ± 0.173
0.735MetGlu: 0.735 ± 0.236
0.473MetPhe: 0.473 ± 0.165
1.103MetGly: 1.103 ± 0.254
0.315MetHis: 0.315 ± 0.107
0.788MetIle: 0.788 ± 0.168
0.683MetLys: 0.683 ± 0.183
1.891MetLeu: 1.891 ± 0.328
0.263MetMet: 0.263 ± 0.113
0.42MetAsn: 0.42 ± 0.136
1.733MetPro: 1.733 ± 0.272
0.893MetGln: 0.893 ± 0.368
1.576MetArg: 1.576 ± 0.316
1.208MetSer: 1.208 ± 0.218
3.624MetThr: 3.624 ± 0.424
1.313MetVal: 1.313 ± 0.307
0.473MetTrp: 0.473 ± 0.137
0.263MetTyr: 0.263 ± 0.13
0.0MetXaa: 0.0 ± 0.0
Asn
3.992AsnAla: 3.992 ± 0.572
0.473AsnCys: 0.473 ± 0.16
2.048AsnAsp: 2.048 ± 0.327
1.471AsnGlu: 1.471 ± 0.294
0.578AsnPhe: 0.578 ± 0.164
2.731AsnGly: 2.731 ± 0.367
0.578AsnHis: 0.578 ± 0.17
0.893AsnIle: 0.893 ± 0.212
0.42AsnLys: 0.42 ± 0.189
1.996AsnLeu: 1.996 ± 0.396
0.63AsnMet: 0.63 ± 0.185
0.788AsnAsn: 0.788 ± 0.188
2.153AsnPro: 2.153 ± 0.367
0.735AsnGln: 0.735 ± 0.221
1.523AsnArg: 1.523 ± 0.225
0.84AsnSer: 0.84 ± 0.225
1.418AsnThr: 1.418 ± 0.349
1.628AsnVal: 1.628 ± 0.242
0.683AsnTrp: 0.683 ± 0.223
0.578AsnTyr: 0.578 ± 0.149
0.0AsnXaa: 0.0 ± 0.0
Pro
8.508ProAla: 8.508 ± 0.831
0.21ProCys: 0.21 ± 0.121
5.41ProAsp: 5.41 ± 0.709
3.204ProGlu: 3.204 ± 0.555
1.628ProPhe: 1.628 ± 0.281
5.2ProGly: 5.2 ± 0.44
1.261ProHis: 1.261 ± 0.303
3.729ProIle: 3.729 ± 0.424
1.628ProLys: 1.628 ± 0.318
4.044ProLeu: 4.044 ± 0.434
1.366ProMet: 1.366 ± 0.237
1.786ProAsn: 1.786 ± 0.297
4.884ProPro: 4.884 ± 0.739
2.048ProGln: 2.048 ± 0.387
3.414ProArg: 3.414 ± 0.471
3.309ProSer: 3.309 ± 0.426
4.044ProThr: 4.044 ± 0.488
4.727ProVal: 4.727 ± 0.525
1.05ProTrp: 1.05 ± 0.226
1.208ProTyr: 1.208 ± 0.232
0.0ProXaa: 0.0 ± 0.0
Gln
5.147GlnAla: 5.147 ± 0.512
0.263GlnCys: 0.263 ± 0.119
1.208GlnAsp: 1.208 ± 0.3
0.893GlnGlu: 0.893 ± 0.223
1.366GlnPhe: 1.366 ± 0.241
1.733GlnGly: 1.733 ± 0.258
0.735GlnHis: 0.735 ± 0.19
1.891GlnIle: 1.891 ± 0.372
0.998GlnLys: 0.998 ± 0.181
3.519GlnLeu: 3.519 ± 0.385
0.945GlnMet: 0.945 ± 0.218
0.945GlnAsn: 0.945 ± 0.228
2.731GlnPro: 2.731 ± 0.381
2.731GlnGln: 2.731 ± 0.42
4.044GlnArg: 4.044 ± 0.526
1.471GlnSer: 1.471 ± 0.337
2.679GlnThr: 2.679 ± 0.352
2.784GlnVal: 2.784 ± 0.373
0.893GlnTrp: 0.893 ± 0.204
0.788GlnTyr: 0.788 ± 0.168
0.0GlnXaa: 0.0 ± 0.0
Arg
9.191ArgAla: 9.191 ± 0.739
0.63ArgCys: 0.63 ± 0.209
4.517ArgAsp: 4.517 ± 0.494
4.097ArgGlu: 4.097 ± 0.497
1.943ArgPhe: 1.943 ± 0.371
5.095ArgGly: 5.095 ± 0.578
1.523ArgHis: 1.523 ± 0.344
4.097ArgIle: 4.097 ± 0.497
1.891ArgLys: 1.891 ± 0.318
5.147ArgLeu: 5.147 ± 0.482
2.048ArgMet: 2.048 ± 0.245
1.943ArgAsn: 1.943 ± 0.325
3.676ArgPro: 3.676 ± 0.511
2.206ArgGln: 2.206 ± 0.297
8.613ArgArg: 8.613 ± 1.157
3.519ArgSer: 3.519 ± 0.42
4.097ArgThr: 4.097 ± 0.52
5.83ArgVal: 5.83 ± 0.822
1.628ArgTrp: 1.628 ± 0.363
1.996ArgTyr: 1.996 ± 0.338
0.0ArgXaa: 0.0 ± 0.0
Ser
5.305SerAla: 5.305 ± 0.814
0.473SerCys: 0.473 ± 0.19
3.729SerAsp: 3.729 ± 0.483
2.416SerGlu: 2.416 ± 0.448
1.103SerPhe: 1.103 ± 0.25
5.41SerGly: 5.41 ± 0.79
0.788SerHis: 0.788 ± 0.21
2.048SerIle: 2.048 ± 0.448
1.261SerLys: 1.261 ± 0.223
3.361SerLeu: 3.361 ± 0.424
1.103SerMet: 1.103 ± 0.235
1.103SerAsn: 1.103 ± 0.258
2.941SerPro: 2.941 ± 0.343
0.998SerGln: 0.998 ± 0.282
3.466SerArg: 3.466 ± 0.532
2.784SerSer: 2.784 ± 0.557
4.044SerThr: 4.044 ± 0.489
3.782SerVal: 3.782 ± 0.389
1.05SerTrp: 1.05 ± 0.227
1.261SerTyr: 1.261 ± 0.263
0.0SerXaa: 0.0 ± 0.0
Thr
10.242ThrAla: 10.242 ± 1.027
0.945ThrCys: 0.945 ± 0.275
5.41ThrAsp: 5.41 ± 0.526
2.941ThrGlu: 2.941 ± 0.437
1.366ThrPhe: 1.366 ± 0.291
6.04ThrGly: 6.04 ± 0.585
1.261ThrHis: 1.261 ± 0.297
2.941ThrIle: 2.941 ± 0.476
1.786ThrLys: 1.786 ± 0.297
6.355ThrLeu: 6.355 ± 0.481
1.418ThrMet: 1.418 ± 0.238
1.523ThrAsn: 1.523 ± 0.294
4.832ThrPro: 4.832 ± 0.391
1.418ThrGln: 1.418 ± 0.231
3.887ThrArg: 3.887 ± 0.457
3.939ThrSer: 3.939 ± 0.537
4.989ThrThr: 4.989 ± 0.671
4.832ThrVal: 4.832 ± 0.557
1.05ThrTrp: 1.05 ± 0.212
1.418ThrTyr: 1.418 ± 0.277
0.0ThrXaa: 0.0 ± 0.0
Val
8.876ValAla: 8.876 ± 0.818
0.63ValCys: 0.63 ± 0.221
6.88ValAsp: 6.88 ± 0.579
5.462ValGlu: 5.462 ± 0.674
1.838ValPhe: 1.838 ± 0.287
6.513ValGly: 6.513 ± 0.658
1.733ValHis: 1.733 ± 0.289
3.729ValIle: 3.729 ± 0.375
1.681ValLys: 1.681 ± 0.274
5.882ValLeu: 5.882 ± 0.537
1.103ValMet: 1.103 ± 0.206
2.416ValAsn: 2.416 ± 0.378
3.834ValPro: 3.834 ± 0.521
3.309ValGln: 3.309 ± 0.35
5.042ValArg: 5.042 ± 0.506
3.782ValSer: 3.782 ± 0.545
5.882ValThr: 5.882 ± 0.609
6.197ValVal: 6.197 ± 0.623
2.048ValTrp: 2.048 ± 0.265
2.048ValTyr: 2.048 ± 0.34
0.0ValXaa: 0.0 ± 0.0
Trp
1.996TrpAla: 1.996 ± 0.364
0.263TrpCys: 0.263 ± 0.108
1.208TrpAsp: 1.208 ± 0.253
1.05TrpGlu: 1.05 ± 0.232
0.788TrpPhe: 0.788 ± 0.193
1.208TrpGly: 1.208 ± 0.202
0.42TrpHis: 0.42 ± 0.154
1.103TrpIle: 1.103 ± 0.253
0.735TrpLys: 0.735 ± 0.171
2.153TrpLeu: 2.153 ± 0.318
0.735TrpMet: 0.735 ± 0.208
0.63TrpAsn: 0.63 ± 0.184
1.313TrpPro: 1.313 ± 0.228
1.155TrpGln: 1.155 ± 0.228
1.786TrpArg: 1.786 ± 0.35
1.155TrpSer: 1.155 ± 0.222
1.05TrpThr: 1.05 ± 0.254
1.576TrpVal: 1.576 ± 0.236
0.525TrpTrp: 0.525 ± 0.224
0.525TrpTyr: 0.525 ± 0.162
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.311TyrAla: 2.311 ± 0.376
0.21TyrCys: 0.21 ± 0.093
1.838TyrAsp: 1.838 ± 0.336
1.681TyrGlu: 1.681 ± 0.317
0.735TyrPhe: 0.735 ± 0.172
2.048TyrGly: 2.048 ± 0.316
0.368TyrHis: 0.368 ± 0.132
0.788TyrIle: 0.788 ± 0.165
0.368TyrLys: 0.368 ± 0.138
1.838TyrLeu: 1.838 ± 0.325
0.42TyrMet: 0.42 ± 0.126
0.368TyrAsn: 0.368 ± 0.13
1.208TyrPro: 1.208 ± 0.309
0.473TyrGln: 0.473 ± 0.128
2.048TyrArg: 2.048 ± 0.365
0.788TyrSer: 0.788 ± 0.189
1.208TyrThr: 1.208 ± 0.257
2.258TyrVal: 2.258 ± 0.328
0.473TyrTrp: 0.473 ± 0.15
0.473TyrTyr: 0.473 ± 0.142
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 88 proteins (19041 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski