Bdellovibrio phage phiMH2K (Bacteriophage phiMH2K)

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; Gokushovirinae; Bdellomicrovirus; Bdellovibrio virus MH2K

Average proteome isoelectric point is 8.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9G053|B_BPPHM Internal scaffolding protein VP3 OS=Bdellovibrio phage phiMH2K OX=145579 GN=ORF3 PE=3 SV=1
MM1 pKa = 6.62QLKK4 pKa = 9.78VFSIRR9 pKa = 11.84DD10 pKa = 3.73SKK12 pKa = 9.73TGVYY16 pKa = 7.53GTPFYY21 pKa = 11.08QHH23 pKa = 5.16THH25 pKa = 4.46GQAEE29 pKa = 4.17RR30 pKa = 11.84SFQQLAKK37 pKa = 10.49DD38 pKa = 3.89PQSTVANHH46 pKa = 7.03PEE48 pKa = 4.14DD49 pKa = 4.19FDD51 pKa = 4.74LFHH54 pKa = 8.04LGEE57 pKa = 4.66YY58 pKa = 10.41DD59 pKa = 3.74DD60 pKa = 4.14QTGKK64 pKa = 8.61LTPLDD69 pKa = 4.0TPEE72 pKa = 4.56HH73 pKa = 5.76CVKK76 pKa = 10.8AIDD79 pKa = 5.75LIKK82 pKa = 10.54QQQ84 pKa = 3.53

Molecular weight: 9.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q9G052|C_BPPHM Protein VP5 OS=Bdellovibrio phage phiMH2K OX=145579 GN=ORF5 PE=3 SV=1
MM1 pKa = 7.22KK2 pKa = 9.95RR3 pKa = 11.84KK4 pKa = 9.45PMSRR8 pKa = 11.84KK9 pKa = 9.55ASQKK13 pKa = 7.46TFKK16 pKa = 10.85KK17 pKa = 8.3NTGVQRR23 pKa = 11.84MNHH26 pKa = 5.89LNPRR30 pKa = 11.84AMRR33 pKa = 11.84GGIRR37 pKa = 11.84LL38 pKa = 3.49

Molecular weight: 4.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

1677

38

533

152.5

17.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.619 ± 1.034

0.596 ± 0.228

4.77 ± 0.899

4.711 ± 0.604

4.711 ± 0.924

5.784 ± 0.748

2.862 ± 0.488

4.89 ± 0.565

7.215 ± 1.203

8.408 ± 0.758

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.504 ± 0.358

6.023 ± 0.691

5.546 ± 0.668

6.142 ± 0.621

5.665 ± 1.084

7.275 ± 0.964

8.229 ± 1.646

3.518 ± 0.498

1.431 ± 0.284

3.101 ± 0.81
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski