Mycobacterium phage Rey

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Mclasvirinae; Reyvirus; Mycobacterium virus Rey

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 153 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G1D5D1|G1D5D1_9CAUD Uncharacterized protein OS=Mycobacterium phage Rey OX=1034115 GN=69 PE=4 SV=1
MM1 pKa = 7.56SNLDD5 pKa = 3.81EE6 pKa = 5.33LYY8 pKa = 10.13PIPFVLAEE16 pKa = 4.08QPRR19 pKa = 11.84MFLNFLALHH28 pKa = 5.88SNIPEE33 pKa = 4.61AYY35 pKa = 9.39RR36 pKa = 11.84DD37 pKa = 4.05MIAAWLLEE45 pKa = 4.09YY46 pKa = 9.6NTALGYY52 pKa = 10.42FIGTNYY58 pKa = 10.68GMDD61 pKa = 3.84ALQEE65 pKa = 4.0ADD67 pKa = 4.95LIAGALAQQFLEE79 pKa = 4.73VVAEE83 pKa = 4.03EE84 pKa = 4.67RR85 pKa = 11.84EE86 pKa = 4.23AADD89 pKa = 3.44GQMFDD94 pKa = 3.92SLEE97 pKa = 4.08KK98 pKa = 10.68EE99 pKa = 4.08IFGDD103 pKa = 4.63GLPQQ107 pKa = 3.99

Molecular weight: 12.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G1D5J6|G1D5J6_9CAUD Uncharacterized protein OS=Mycobacterium phage Rey OX=1034115 GN=156 PE=4 SV=1
MM1 pKa = 5.95NTKK4 pKa = 10.19RR5 pKa = 11.84RR6 pKa = 11.84VKK8 pKa = 10.11PKK10 pKa = 8.25QDD12 pKa = 2.86EE13 pKa = 4.02RR14 pKa = 11.84RR15 pKa = 11.84VYY17 pKa = 10.04EE18 pKa = 3.55FHH20 pKa = 7.07IPGRR24 pKa = 11.84SHH26 pKa = 7.28LVPGTEE32 pKa = 3.79VSLEE36 pKa = 4.16GKK38 pKa = 9.35RR39 pKa = 11.84GRR41 pKa = 11.84YY42 pKa = 8.48RR43 pKa = 11.84FQYY46 pKa = 8.78AQKK49 pKa = 8.88PLHH52 pKa = 6.44EE53 pKa = 4.87GRR55 pKa = 11.84PTVLTFVGGRR65 pKa = 11.84LDD67 pKa = 3.64GQGEE71 pKa = 4.49KK72 pKa = 10.43FVAVYY77 pKa = 7.99PWRR80 pKa = 11.84VKK82 pKa = 9.07TVHH85 pKa = 5.94RR86 pKa = 11.84TSKK89 pKa = 9.58TLVNITKK96 pKa = 10.32EE97 pKa = 4.2KK98 pKa = 10.29GSKK101 pKa = 9.5

Molecular weight: 11.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

153

0

153

24213

20

2267

158.3

17.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.015 ± 0.457

1.02 ± 0.149

6.947 ± 0.195

5.989 ± 0.323

3.151 ± 0.126

8.372 ± 0.389

2.044 ± 0.17

4.919 ± 0.178

4.427 ± 0.21

8.02 ± 0.207

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.792 ± 0.161

3.556 ± 0.163

4.96 ± 0.209

3.775 ± 0.194

6.022 ± 0.213

5.563 ± 0.242

6.166 ± 0.214

6.988 ± 0.309

2.3 ± 0.129

2.974 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski