Amino acid dipepetide frequency for Mycobacterium phage Rey

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.069AlaAla: 11.069 ± 0.97
1.033AlaCys: 1.033 ± 0.262
6.484AlaAsp: 6.484 ± 0.427
6.154AlaGlu: 6.154 ± 0.813
2.519AlaPhe: 2.519 ± 0.358
7.641AlaGly: 7.641 ± 1.049
1.9AlaHis: 1.9 ± 0.293
4.543AlaIle: 4.543 ± 0.634
4.832AlaLys: 4.832 ± 0.465
9.128AlaLeu: 9.128 ± 0.734
2.726AlaMet: 2.726 ± 0.299
2.932AlaAsn: 2.932 ± 0.457
4.295AlaPro: 4.295 ± 0.644
4.378AlaGln: 4.378 ± 0.519
5.824AlaArg: 5.824 ± 0.512
5.658AlaSer: 5.658 ± 0.593
6.484AlaThr: 6.484 ± 0.714
7.93AlaVal: 7.93 ± 0.659
1.9AlaTrp: 1.9 ± 0.236
2.726AlaTyr: 2.726 ± 0.335
0.0AlaXaa: 0.0 ± 0.0
Cys
0.909CysAla: 0.909 ± 0.225
0.124CysCys: 0.124 ± 0.068
0.661CysAsp: 0.661 ± 0.206
0.661CysGlu: 0.661 ± 0.169
0.083CysPhe: 0.083 ± 0.054
0.867CysGly: 0.867 ± 0.264
0.041CysHis: 0.041 ± 0.043
0.413CysIle: 0.413 ± 0.146
0.372CysLys: 0.372 ± 0.132
0.496CysLeu: 0.496 ± 0.14
0.413CysMet: 0.413 ± 0.138
0.496CysAsn: 0.496 ± 0.14
0.909CysPro: 0.909 ± 0.23
0.454CysGln: 0.454 ± 0.133
0.743CysArg: 0.743 ± 0.173
0.909CysSer: 0.909 ± 0.215
0.454CysThr: 0.454 ± 0.125
0.496CysVal: 0.496 ± 0.139
0.289CysTrp: 0.289 ± 0.107
0.413CysTyr: 0.413 ± 0.152
0.0CysXaa: 0.0 ± 0.0
Asp
6.732AspAla: 6.732 ± 0.607
0.867AspCys: 0.867 ± 0.185
5.287AspAsp: 5.287 ± 0.473
4.667AspGlu: 4.667 ± 0.516
2.23AspPhe: 2.23 ± 0.346
7.104AspGly: 7.104 ± 0.552
1.735AspHis: 1.735 ± 0.292
3.015AspIle: 3.015 ± 0.432
2.478AspLys: 2.478 ± 0.365
6.113AspLeu: 6.113 ± 0.488
1.693AspMet: 1.693 ± 0.281
1.776AspAsn: 1.776 ± 0.272
4.585AspPro: 4.585 ± 0.407
2.354AspGln: 2.354 ± 0.303
3.717AspArg: 3.717 ± 0.411
3.593AspSer: 3.593 ± 0.388
3.345AspThr: 3.345 ± 0.42
4.006AspVal: 4.006 ± 0.384
1.9AspTrp: 1.9 ± 0.382
2.272AspTyr: 2.272 ± 0.341
0.0AspXaa: 0.0 ± 0.0
Glu
6.526GluAla: 6.526 ± 0.505
0.413GluCys: 0.413 ± 0.152
4.213GluAsp: 4.213 ± 0.558
2.974GluGlu: 2.974 ± 0.462
2.891GluPhe: 2.891 ± 0.454
4.254GluGly: 4.254 ± 0.443
1.156GluHis: 1.156 ± 0.227
3.676GluIle: 3.676 ± 0.439
2.148GluLys: 2.148 ± 0.384
5.204GluLeu: 5.204 ± 0.512
2.148GluMet: 2.148 ± 0.352
2.148GluAsn: 2.148 ± 0.271
2.354GluPro: 2.354 ± 0.337
2.561GluGln: 2.561 ± 0.361
4.13GluArg: 4.13 ± 0.359
2.643GluSer: 2.643 ± 0.307
2.809GluThr: 2.809 ± 0.426
4.048GluVal: 4.048 ± 0.419
2.024GluTrp: 2.024 ± 0.337
1.569GluTyr: 1.569 ± 0.259
0.0GluXaa: 0.0 ± 0.0
Phe
2.891PheAla: 2.891 ± 0.361
0.413PheCys: 0.413 ± 0.148
3.304PheAsp: 3.304 ± 0.402
1.487PheGlu: 1.487 ± 0.242
0.95PhePhe: 0.95 ± 0.202
2.809PheGly: 2.809 ± 0.408
0.413PheHis: 0.413 ± 0.145
1.569PheIle: 1.569 ± 0.276
1.735PheLys: 1.735 ± 0.291
2.561PheLeu: 2.561 ± 0.368
0.578PheMet: 0.578 ± 0.174
1.487PheAsn: 1.487 ± 0.211
1.363PhePro: 1.363 ± 0.184
1.239PheGln: 1.239 ± 0.221
1.404PheArg: 1.404 ± 0.261
1.693PheSer: 1.693 ± 0.234
2.396PheThr: 2.396 ± 0.326
2.148PheVal: 2.148 ± 0.306
0.454PheTrp: 0.454 ± 0.145
0.62PheTyr: 0.62 ± 0.144
0.0PheXaa: 0.0 ± 0.0
Gly
7.021GlyAla: 7.021 ± 1.104
0.991GlyCys: 0.991 ± 0.208
5.493GlyAsp: 5.493 ± 0.53
4.337GlyGlu: 4.337 ± 0.395
2.272GlyPhe: 2.272 ± 0.267
9.004GlyGly: 9.004 ± 1.362
1.446GlyHis: 1.446 ± 0.262
4.171GlyIle: 4.171 ± 0.573
4.089GlyLys: 4.089 ± 0.488
6.402GlyLeu: 6.402 ± 0.565
2.685GlyMet: 2.685 ± 0.358
3.924GlyAsn: 3.924 ± 0.381
3.345GlyPro: 3.345 ± 0.567
3.139GlyGln: 3.139 ± 0.408
4.13GlyArg: 4.13 ± 0.499
5.328GlySer: 5.328 ± 0.78
5.617GlyThr: 5.617 ± 0.536
5.452GlyVal: 5.452 ± 0.456
2.148GlyTrp: 2.148 ± 0.361
2.685GlyTyr: 2.685 ± 0.288
0.0GlyXaa: 0.0 ± 0.0
His
1.652HisAla: 1.652 ± 0.327
0.372HisCys: 0.372 ± 0.135
1.693HisAsp: 1.693 ± 0.304
0.991HisGlu: 0.991 ± 0.187
0.661HisPhe: 0.661 ± 0.17
1.776HisGly: 1.776 ± 0.227
0.33HisHis: 0.33 ± 0.127
1.528HisIle: 1.528 ± 0.276
0.62HisLys: 0.62 ± 0.149
1.446HisLeu: 1.446 ± 0.271
0.661HisMet: 0.661 ± 0.186
0.743HisAsn: 0.743 ± 0.185
0.909HisPro: 0.909 ± 0.196
0.743HisGln: 0.743 ± 0.221
1.115HisArg: 1.115 ± 0.278
0.991HisSer: 0.991 ± 0.238
1.9HisThr: 1.9 ± 0.284
0.991HisVal: 0.991 ± 0.234
0.496HisTrp: 0.496 ± 0.16
0.826HisTyr: 0.826 ± 0.205
0.0HisXaa: 0.0 ± 0.0
Ile
4.791IleAla: 4.791 ± 0.547
0.372IleCys: 0.372 ± 0.109
4.419IleAsp: 4.419 ± 0.418
3.18IleGlu: 3.18 ± 0.442
1.446IlePhe: 1.446 ± 0.257
4.543IleGly: 4.543 ± 0.628
0.867IleHis: 0.867 ± 0.23
1.9IleIle: 1.9 ± 0.286
2.354IleLys: 2.354 ± 0.369
3.841IleLeu: 3.841 ± 0.416
1.074IleMet: 1.074 ± 0.234
1.735IleAsn: 1.735 ± 0.288
3.18IlePro: 3.18 ± 0.38
1.528IleGln: 1.528 ± 0.233
3.222IleArg: 3.222 ± 0.337
2.561IleSer: 2.561 ± 0.352
3.387IleThr: 3.387 ± 0.405
3.304IleVal: 3.304 ± 0.475
0.62IleTrp: 0.62 ± 0.181
0.867IleTyr: 0.867 ± 0.184
0.0IleXaa: 0.0 ± 0.0
Lys
4.667LysAla: 4.667 ± 0.489
0.537LysCys: 0.537 ± 0.203
2.478LysAsp: 2.478 ± 0.396
1.982LysGlu: 1.982 ± 0.266
1.446LysPhe: 1.446 ± 0.251
3.387LysGly: 3.387 ± 0.444
0.909LysHis: 0.909 ± 0.207
2.272LysIle: 2.272 ± 0.348
2.726LysLys: 2.726 ± 0.387
3.676LysLeu: 3.676 ± 0.37
0.991LysMet: 0.991 ± 0.249
1.941LysAsn: 1.941 ± 0.295
2.024LysPro: 2.024 ± 0.382
1.652LysGln: 1.652 ± 0.326
2.85LysArg: 2.85 ± 0.376
1.693LysSer: 1.693 ± 0.29
2.478LysThr: 2.478 ± 0.378
3.511LysVal: 3.511 ± 0.392
1.28LysTrp: 1.28 ± 0.186
1.776LysTyr: 1.776 ± 0.296
0.0LysXaa: 0.0 ± 0.0
Leu
9.128LeuAla: 9.128 ± 0.575
0.743LeuCys: 0.743 ± 0.232
5.824LeuAsp: 5.824 ± 0.537
4.791LeuGlu: 4.791 ± 0.51
2.354LeuPhe: 2.354 ± 0.313
6.526LeuGly: 6.526 ± 0.781
1.363LeuHis: 1.363 ± 0.254
3.552LeuIle: 3.552 ± 0.413
3.139LeuLys: 3.139 ± 0.361
5.493LeuLeu: 5.493 ± 0.569
2.148LeuMet: 2.148 ± 0.302
3.098LeuAsn: 3.098 ± 0.538
4.378LeuPro: 4.378 ± 0.479
3.139LeuGln: 3.139 ± 0.345
4.998LeuArg: 4.998 ± 0.501
4.956LeuSer: 4.956 ± 0.53
4.708LeuThr: 4.708 ± 0.424
6.319LeuVal: 6.319 ± 0.67
1.528LeuTrp: 1.528 ± 0.227
2.024LeuTyr: 2.024 ± 0.31
0.0LeuXaa: 0.0 ± 0.0
Met
3.304MetAla: 3.304 ± 0.33
0.289MetCys: 0.289 ± 0.092
1.404MetAsp: 1.404 ± 0.242
1.776MetGlu: 1.776 ± 0.241
0.702MetPhe: 0.702 ± 0.208
1.817MetGly: 1.817 ± 0.281
0.454MetHis: 0.454 ± 0.148
1.156MetIle: 1.156 ± 0.239
1.198MetLys: 1.198 ± 0.23
1.693MetLeu: 1.693 ± 0.319
0.537MetMet: 0.537 ± 0.143
1.074MetAsn: 1.074 ± 0.202
1.693MetPro: 1.693 ± 0.256
0.867MetGln: 0.867 ± 0.2
1.446MetArg: 1.446 ± 0.25
3.345MetSer: 3.345 ± 0.508
2.478MetThr: 2.478 ± 0.315
1.404MetVal: 1.404 ± 0.301
0.578MetTrp: 0.578 ± 0.177
0.702MetTyr: 0.702 ± 0.181
0.0MetXaa: 0.0 ± 0.0
Asn
3.098AsnAla: 3.098 ± 0.74
0.248AsnCys: 0.248 ± 0.099
1.859AsnAsp: 1.859 ± 0.293
1.528AsnGlu: 1.528 ± 0.266
1.074AsnPhe: 1.074 ± 0.233
3.635AsnGly: 3.635 ± 0.407
0.867AsnHis: 0.867 ± 0.217
1.693AsnIle: 1.693 ± 0.265
1.652AsnLys: 1.652 ± 0.241
2.85AsnLeu: 2.85 ± 0.29
1.033AsnMet: 1.033 ± 0.214
1.322AsnAsn: 1.322 ± 0.251
2.437AsnPro: 2.437 ± 0.326
1.528AsnGln: 1.528 ± 0.271
2.437AsnArg: 2.437 ± 0.322
2.272AsnSer: 2.272 ± 0.292
1.693AsnThr: 1.693 ± 0.261
2.437AsnVal: 2.437 ± 0.293
0.578AsnTrp: 0.578 ± 0.155
1.322AsnTyr: 1.322 ± 0.225
0.0AsnXaa: 0.0 ± 0.0
Pro
5.245ProAla: 5.245 ± 0.822
0.33ProCys: 0.33 ± 0.116
3.593ProAsp: 3.593 ± 0.423
3.593ProGlu: 3.593 ± 0.515
1.776ProPhe: 1.776 ± 0.228
4.585ProGly: 4.585 ± 0.547
1.528ProHis: 1.528 ± 0.257
1.817ProIle: 1.817 ± 0.306
1.941ProLys: 1.941 ± 0.343
3.552ProLeu: 3.552 ± 0.382
1.487ProMet: 1.487 ± 0.222
1.652ProAsn: 1.652 ± 0.24
1.941ProPro: 1.941 ± 0.332
1.859ProGln: 1.859 ± 0.308
2.767ProArg: 2.767 ± 0.34
2.189ProSer: 2.189 ± 0.39
2.519ProThr: 2.519 ± 0.361
4.75ProVal: 4.75 ± 0.436
1.074ProTrp: 1.074 ± 0.194
1.404ProTyr: 1.404 ± 0.242
0.0ProXaa: 0.0 ± 0.0
Gln
4.13GlnAla: 4.13 ± 0.563
0.248GlnCys: 0.248 ± 0.111
1.941GlnAsp: 1.941 ± 0.313
1.446GlnGlu: 1.446 ± 0.227
1.363GlnPhe: 1.363 ± 0.199
2.396GlnGly: 2.396 ± 0.304
1.074GlnHis: 1.074 ± 0.21
2.602GlnIle: 2.602 ± 0.257
1.569GlnLys: 1.569 ± 0.228
3.593GlnLeu: 3.593 ± 0.452
1.404GlnMet: 1.404 ± 0.223
1.404GlnAsn: 1.404 ± 0.273
1.611GlnPro: 1.611 ± 0.325
1.735GlnGln: 1.735 ± 0.286
2.891GlnArg: 2.891 ± 0.41
1.693GlnSer: 1.693 ± 0.281
1.941GlnThr: 1.941 ± 0.293
2.437GlnVal: 2.437 ± 0.303
1.074GlnTrp: 1.074 ± 0.209
1.198GlnTyr: 1.198 ± 0.229
0.0GlnXaa: 0.0 ± 0.0
Arg
4.874ArgAla: 4.874 ± 0.375
0.661ArgCys: 0.661 ± 0.191
4.048ArgAsp: 4.048 ± 0.437
4.378ArgGlu: 4.378 ± 0.383
2.189ArgPhe: 2.189 ± 0.321
3.924ArgGly: 3.924 ± 0.368
1.074ArgHis: 1.074 ± 0.239
4.089ArgIle: 4.089 ± 0.36
3.18ArgLys: 3.18 ± 0.422
4.667ArgLeu: 4.667 ± 0.445
1.982ArgMet: 1.982 ± 0.381
1.9ArgAsn: 1.9 ± 0.252
2.767ArgPro: 2.767 ± 0.422
2.809ArgGln: 2.809 ± 0.364
3.882ArgArg: 3.882 ± 0.489
3.387ArgSer: 3.387 ± 0.443
2.932ArgThr: 2.932 ± 0.311
4.461ArgVal: 4.461 ± 0.535
1.198ArgTrp: 1.198 ± 0.201
1.817ArgTyr: 1.817 ± 0.239
0.0ArgXaa: 0.0 ± 0.0
Ser
5.658SerAla: 5.658 ± 0.529
0.578SerCys: 0.578 ± 0.179
4.048SerAsp: 4.048 ± 0.474
2.974SerGlu: 2.974 ± 0.353
1.569SerPhe: 1.569 ± 0.265
4.708SerGly: 4.708 ± 0.665
0.826SerHis: 0.826 ± 0.217
2.437SerIle: 2.437 ± 0.316
2.354SerLys: 2.354 ± 0.265
4.089SerLeu: 4.089 ± 0.426
2.065SerMet: 2.065 ± 0.269
2.313SerAsn: 2.313 ± 0.362
2.396SerPro: 2.396 ± 0.325
1.9SerGln: 1.9 ± 0.272
3.304SerArg: 3.304 ± 0.415
3.263SerSer: 3.263 ± 0.484
3.841SerThr: 3.841 ± 0.389
3.593SerVal: 3.593 ± 0.423
1.652SerTrp: 1.652 ± 0.274
2.065SerTyr: 2.065 ± 0.287
0.0SerXaa: 0.0 ± 0.0
Thr
6.567ThrAla: 6.567 ± 0.724
0.496ThrCys: 0.496 ± 0.152
3.882ThrAsp: 3.882 ± 0.434
4.171ThrGlu: 4.171 ± 0.48
2.024ThrPhe: 2.024 ± 0.348
5.534ThrGly: 5.534 ± 0.543
1.198ThrHis: 1.198 ± 0.202
3.428ThrIle: 3.428 ± 0.439
2.932ThrLys: 2.932 ± 0.367
5.163ThrLeu: 5.163 ± 0.519
1.404ThrMet: 1.404 ± 0.264
1.693ThrAsn: 1.693 ± 0.253
2.932ThrPro: 2.932 ± 0.411
1.363ThrGln: 1.363 ± 0.212
3.304ThrArg: 3.304 ± 0.367
2.891ThrSer: 2.891 ± 0.361
4.667ThrThr: 4.667 ± 0.517
4.585ThrVal: 4.585 ± 0.522
1.569ThrTrp: 1.569 ± 0.291
1.859ThrTyr: 1.859 ± 0.303
0.0ThrXaa: 0.0 ± 0.0
Val
6.897ValAla: 6.897 ± 0.584
0.743ValCys: 0.743 ± 0.192
5.534ValAsp: 5.534 ± 0.524
4.419ValGlu: 4.419 ± 0.488
2.106ValPhe: 2.106 ± 0.266
5.741ValGly: 5.741 ± 0.428
1.735ValHis: 1.735 ± 0.371
3.18ValIle: 3.18 ± 0.46
2.809ValLys: 2.809 ± 0.319
5.741ValLeu: 5.741 ± 0.539
1.446ValMet: 1.446 ± 0.248
2.23ValAsn: 2.23 ± 0.418
3.593ValPro: 3.593 ± 0.299
2.685ValGln: 2.685 ± 0.269
4.13ValArg: 4.13 ± 0.408
4.006ValSer: 4.006 ± 0.403
4.502ValThr: 4.502 ± 0.576
4.915ValVal: 4.915 ± 0.629
1.487ValTrp: 1.487 ± 0.262
1.982ValTyr: 1.982 ± 0.374
0.0ValXaa: 0.0 ± 0.0
Trp
1.982TrpAla: 1.982 ± 0.287
0.33TrpCys: 0.33 ± 0.15
1.404TrpAsp: 1.404 ± 0.226
1.9TrpGlu: 1.9 ± 0.264
0.785TrpPhe: 0.785 ± 0.217
1.033TrpGly: 1.033 ± 0.19
0.661TrpHis: 0.661 ± 0.218
0.95TrpIle: 0.95 ± 0.184
0.743TrpLys: 0.743 ± 0.163
2.437TrpLeu: 2.437 ± 0.283
0.785TrpMet: 0.785 ± 0.174
0.62TrpAsn: 0.62 ± 0.184
1.156TrpPro: 1.156 ± 0.218
0.909TrpGln: 0.909 ± 0.172
1.817TrpArg: 1.817 ± 0.295
1.404TrpSer: 1.404 ± 0.212
1.528TrpThr: 1.528 ± 0.258
1.239TrpVal: 1.239 ± 0.238
0.702TrpTrp: 0.702 ± 0.182
0.62TrpTyr: 0.62 ± 0.169
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.015TyrAla: 3.015 ± 0.362
0.413TyrCys: 0.413 ± 0.149
1.9TyrAsp: 1.9 ± 0.291
2.478TyrGlu: 2.478 ± 0.329
1.074TyrPhe: 1.074 ± 0.203
2.437TyrGly: 2.437 ± 0.318
0.867TyrHis: 0.867 ± 0.175
1.198TyrIle: 1.198 ± 0.185
1.404TyrLys: 1.404 ± 0.247
2.065TyrLeu: 2.065 ± 0.307
0.62TyrMet: 0.62 ± 0.176
1.074TyrAsn: 1.074 ± 0.239
1.735TyrPro: 1.735 ± 0.38
0.909TyrGln: 0.909 ± 0.217
2.106TyrArg: 2.106 ± 0.249
1.156TyrSer: 1.156 ± 0.228
1.982TyrThr: 1.982 ± 0.293
1.859TyrVal: 1.859 ± 0.31
0.454TyrTrp: 0.454 ± 0.134
0.991TyrTyr: 0.991 ± 0.206
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 153 proteins (24213 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski